{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"US_2012_0082978_A1","frontPageModel":{"patentViewModel":{"ref":{"entityRefType":"PATENT","entityRefId":"127-667-334-288-990"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":11650,"type":"PATENT","title":"University of Alberta - Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":3172,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8316,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately:uni* alberta
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Add to collection. Total patents: 3178
Search Applicants and Owners separately:uni* alberta
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Select all patents in the collection and expand by simple families.
Add to collection. Total patents: 3178
a) Said optical imaging device transmits said digital representation to said computer readable memory;\n
b) Said computer scans said digital representation at low-resolution for cell-like objects;\n
c) Said computer creates a listing, capable of being referenced by the computer at some later time, of each cell-like object in the digital representation thereby generating a list of salient areas;\n
d) The computer chooses a salient area from the first element in said list of salient areas;\n
e) The computer retrieves the portion of said digital representation which contains at least said salient area for analysis and performs digital processing on said portion of the digital representation so as to identify, locate and store within said computer readable memory the location of at least one probe present in said portion of the digital representation;\n
f) The computer then chooses the next element in said list of salient areas, performing step e) above;\n
g) Step f) is repeated until a sufficient number of salient areas have been analysed, said sufficient number determined at the option of the computer system or by intervention of a human operator;\n
h) The system uses the location or at least one probe in each of the analyzed salient areas to determine the relationship of probes within each salient area; and\n
i) Said relationship of probes are used to generate a set of population statistics that may be used for analyzing the condition of said cells of interest."],"number":1,"annotation":false,"title":false,"claim":true},{"lines":["A method of immobilizing cells in a microfluidic channel and preparing said cells for use in cellular analysis comprising\n
Taking a population of cells of interest, suspended in a fluid;\n
Filling a microfluidic channel with said population of cells of interest suspended in a fluid; and\n
Raising the temperature of said microfluidic channel to 55-95° C. for a period of time sufficient to allow immobilization of a portion of said population of cells of interest to said microfluidic channel;\n
Wherein time sufficient to allow immobilization of a portion of said population of cells of interest is determined by intervention of a human operator as the immobilization of a certain portion of cells of interest, either in terms of net number of cells immobilized, or alternatively as a percentage of total cells present in said population of cells of interest."],"number":2,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 2 wherein the cellular analysis is FISH."],"number":3,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 2 wherein the fluid is a buffer suitable for maintaining the size and shape of the individual cells making up the population of cells of interest."],"number":4,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 4 wherein the buffer is 1× Phosphate Buffered Saline (PBS)."],"number":5,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 4 wherein the temperature is raised to 75-85° C."],"number":6,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 6 wherein the temperature is raised to 75-85° C. for a period of 10 minutes."],"number":7,"annotation":false,"title":false,"claim":true},{"lines":["A method of increasing the portion of cells of interest immobilized within a microfluidic channel comprising\n
Having at least one region within said microfluidic channel with a course surface;\n
Taking a population of cells of interest, suspended in a fluid;\n
Filling a microfluidic channel with said population of cells of interest suspended in a fluid so as to allow a portion of said population of cells of interest to come into fluid contact with said course surface of said microfluidic channel;\n
Raising the temperature of said microfluidic channel to 55-95° C. for a period of time sufficient to allow immobilization of a portion of said population of cells of interest to said microfluidic channel;\n
Wherein time sufficient to allow immobilization of a portion of said population of cells of interest is determined by intervention of a human operator as the immobilization of a certain portion of cells of interest, either in terms of net number of cells immobilized, or alternatively as a percentage of total cells present in said population of cells of interest; and"],"number":8,"annotation":false,"title":false,"claim":true},{"lines":["An apparatus for performing cellular analysis comprising\n
A first access port/well;\n
A second access port/well; and\n
At least one microfluidic channel;\n
Wherein said first access port/well is in fluid communication with said second access port/well by means of said at least one microfluidic channel;\n
And wherein said at least one microfluidic channel is of dimensions no greater than 110 μm×620 μm×100 mm."],"number":9,"annotation":false,"title":false,"claim":true},{"lines":["The apparatus of claim 9 wherein the dimensions of the microfluidic channels are 55 μm×310 μm×50 mm."],"number":10,"annotation":false,"title":false,"claim":true},{"lines":["The apparatus of claim 10 wherein the first access port/well and second access port/well each have a volume of 1.5 μL."],"number":11,"annotation":false,"title":false,"claim":true},{"lines":["The apparatus of claim 10 wherein said microfluidic channels and said first and second access ports/wells are formed by etching a planar glass surface."],"number":12,"annotation":false,"title":false,"claim":true},{"lines":["The apparatus of claim 10 wherein said microfluidic channels and said first and second access ports/wells are formed by the interface between a planar glass surface and a moulded flexible, plastic."],"number":13,"annotation":false,"title":false,"claim":true},{"lines":["The apparatus of claim 13, wherein said moulded, flexible plastic is PDMS."],"number":14,"annotation":false,"title":false,"claim":true}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}