{"search_session":{},"preferences":{"l":"ru","queryLanguage":"ru"},"patentId":"US_2012_0070827_A1","frontPageModel":{"patentViewModel":{"ref":{"entityRefId":"114-050-210-560-436","entityRefType":"PATENT"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":10800,"type":"PATENT","title":"Aarhus University - Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":1140,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8265,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately: \"Aarhus Univ*\"
Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 786
Search Applicants and Owners separately: \"Aarhus Univ*\"
Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 786
a. identifying the target antigenic material,\n
b. contacting said target antigenic material with a binding member generating system and\n
c. isolating binding member(s) recognising the target antigenic material."],"number":1,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 1 including a step of;\n
d. minimizing the number binding members isolated recognizing non-target antigenic material."],"number":2,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 1, wherein the binding member generating system allows linkage between the genotype and the phenotype of binding members generated by the system."],"number":3,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 1, wherein the binding member generating system allowing linkage between the genotype and the phenotype of the binding member is selected from the group of; ribosome display, DNA display (including cis display), yeast display, bacterial display, retroviral display and phage display."],"number":4,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 1, wherein the binding member generating system is phage display."],"number":5,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 1, wherein the ratio of target antigenic material\nRTA=targetantigenmaterialnon-targetantigenmaterial\nis less than 1/1000."],"number":6,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 1, wherein the target antigenic material may be limited to one cell."],"number":7,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 1, wherein the target antigenic material is a cell."],"number":8,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 1, wherein the target antigenic material is a cell in suspension."],"number":9,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 1, wherein the target antigenic material is a cell present on a slide."],"number":10,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 1, wherein the target antigenic material is a fetal cell."],"number":11,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 1, wherein the minimizing step comprises UV irradiation."],"number":12,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 12, wherein the antigenic material and its associated binding members are protected from UV irradiation by shielding."],"number":13,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 13, wherein shielding is obtained by use of an item selected from the group of; a puck, a shadow-stick."],"number":14,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 14, wherein a cylinder is used for minimizing reflection of UV light."],"number":15,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 1, wherein the binding member is selected from the group of antibodies, antibody fragments and non-immune binding members."],"number":16,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 16, wherein the binding member is an antibody."],"number":17,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 16, wherein the binding member is an antibody fragment."],"number":18,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 16, wherein the binding member is a non-immune binding member."],"number":19,"annotation":false,"claim":true,"title":false},{"lines":["A fetal cell antigen recognised by a binding member isolated using the method of claim 1."],"number":20,"annotation":false,"claim":true,"title":false},{"lines":["Binding members recognising a fetal cell antigen according to claim 20."],"number":21,"annotation":false,"claim":true,"title":false},{"lines":["An assay method comprising the steps of;\n
a. providing a maternal blood sample,\n
b. selectively labelling at least one fetal cell by labelling of a fetal cell antigen according to claim 20\n"],"number":22,"annotation":false,"claim":true,"title":false},{"lines":["An assay method comprising the steps of:\nin said blood sample.\n
a. providing a maternal blood sample,\n
b. selectively labelling at least one fetal cell by use of a binding member according to claim 21\n"],"number":23,"annotation":false,"claim":true,"title":false},{"lines":["The assay according to claim 22, wherein said selectively labelled fetal cell is labelled by an immunodetection technique."],"number":24,"annotation":false,"claim":true,"title":false},{"lines":["The assay according to claim 23, wherein said selectively labelled fetal cell is labelled by an immunodetection technique."],"number":25,"annotation":false,"claim":true,"title":false},{"lines":["The assay according to claim 24, wherein the immunodetection technique comprises use of a binding member according to claim 21."],"number":26,"annotation":false,"claim":true,"title":false},{"lines":["The assay according to claim 25, wherein the immunodetection technique comprises use of a binding member according to claim 21."],"number":27,"annotation":false,"claim":true,"title":false},{"lines":["The assay according to claim 22, comprising one or more of the characteristics of the method of detecting at least on fetal cell, said method comprising the following steps of:\nin said blood sample.\n
a. providing a maternal blood sample,\n
b. fixing and permealizing nucleated cells present in said blood sample while;\n\ni. maintaining cell morphology,\nii. maintaining protein content of the cells and\niii. ensuring accessibility for a hybridisation probe,\n
c. adding a hybridisation probe and\n
d. detecting at least one fetal cell"],"number":28,"annotation":false,"claim":true,"title":false},{"lines":["The assay according to claim 22, wherein said selectively labelled fetal cell is isolated from the sample."],"number":29,"annotation":false,"claim":true,"title":false},{"lines":["The assay according to claim 27, wherein said selectively labelled fetal cell is isolated from the sample by FACS analysis."],"number":30,"annotation":false,"claim":true,"title":false},{"lines":["The assay according to claim 27, wherein said selectively labelled fetal cell is isolated from the sample by MACS analysis."],"number":31,"annotation":false,"claim":true,"title":false},{"lines":["The assay according to claim 27, wherein said selectively labelled fetal cell is subject to in situ chromosomal DNA analysis."],"number":32,"annotation":false,"claim":true,"title":false},{"lines":["The assay according to claim 22 for determination of gender."],"number":33,"annotation":false,"claim":true,"title":false},{"lines":["The assay according to claim 22 for determination of chromosomal abnormality."],"number":34,"annotation":false,"claim":true,"title":false},{"lines":["The assay according to claim 33, wherein gender or chromosomal abnormality is determined by a DNA hybridisation technique."],"number":35,"annotation":false,"claim":true,"title":false},{"lines":["The assay according to claim 35, wherein the DNA hybridisation technique is selected from the group of; fluorescent in situ hybridization (FISH), primed in situ labeling (PRINS), quantitative FISH (Q-FISH) and multicolor-banding (MCB)."],"number":36,"annotation":false,"claim":true,"title":false},{"lines":["The assay according to claim 36, wherein the DNA hybridisation technique is FISH."],"number":37,"annotation":false,"claim":true,"title":false},{"lines":["The assay according to claim 33, wherein gender or chromosomal abnormality is determined by PCR."],"number":38,"annotation":false,"claim":true,"title":false},{"lines":["The assay according to claim 34, wherein gender or chromosomal abnormality is determined by PCR."],"number":39,"annotation":false,"claim":true,"title":false},{"lines":["The assay according to claim 22, wherein the maternal blood sample is from a pregnant woman at 6-15 weeks of gestation."],"number":40,"annotation":false,"claim":true,"title":false}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}