Method To Identify And Predict Disease Progression Of Human Papilloma Virus-infected Lesions

Abstract

The present invention provides a method for distinguishing benign human papilloma virus (HPV)-infected tissue from HPV-related lesions that have undergone malignant transformation. In one embodiment, the invention comprises a simple histochemical staining method and details a novel process for examining HPV-infected cells by determining susceptibility to enzymatic DNA digestion. Residual virion-associated DNA is seen only in benign HPV-infected lesions, while absence of residual DNA is seen with malignant transformation. In another embodiment, the invention comprises immunohistochemical assay methods for examining HPV-infected cells, utilizing antibodies to HPV L1 proteins. These methods can be used to predict biologic behavior of HPV-infected lesions. The invention can improve current cervical cancer screening programs, and improve clinical management of patients by defining malignant potential of HPV-infected tissue more accurately.


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Document History
  • Publication: Apr 21, 2011
  • Application: Dec 20, 2010
    US US 97277510 A
  • Priority: Dec 20, 2010
    US US 97277510 A
  • Priority: Jul 2, 2009
    US US 2009/0049539 W
  • Priority: Jul 3, 2008
    US US 7805008 P

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