{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"US_2011_0020865_A1","frontPageModel":{"patentViewModel":{"ref":{"entityRefId":"136-878-745-813-995","entityRefType":"PATENT"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":11665,"type":"PATENT","title":"University of Nottingham - Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":2682,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8329,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
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(a) a DnaJ-like protein and an Hsp70 family protein; or\n
(b) a DnaJ-like protein and SIL1; or\n
(c) an Hsp70 family protein and SIL1."],"number":33,"annotation":false,"claim":true,"title":false},{"lines":["A host cell suitable for enhanced production of a protein product of choice characterised in that the host cell is genetically modified to cause over-expression of three or more helper proteins, wherein the three or more helper proteins comprise a DnaJ-like protein, an Hsp70 family protein and SIL1, and wherein the DnaJ-like protein is not SCJ1."],"number":34,"annotation":false,"claim":true,"title":false},{"lines":["The host cell of claim 32 wherein the Hsp70 family protein is a protein that localises to the lumen of the ER."],"number":35,"annotation":false,"claim":true,"title":false},{"lines":["The host cell of claim 2 wherein the Hsp70 family protein is not a prokaryotic Hsp70 family protein."],"number":36,"annotation":false,"claim":true,"title":false},{"lines":["The host cell of claim 32 according to any one of the preceding claims wherein the Hsp70 family protein is LHS1, KAR2, SSA1, SSA2, SSA3, SSA4, SSE1, SSE2, SSB1, SSB2 or ECM10."],"number":37,"annotation":false,"claim":true,"title":false},{"lines":["The host cell of claim 32 wherein the DnaJ-like protein is a protein that localises to the ER membrane."],"number":38,"annotation":false,"claim":true,"title":false},{"lines":["The host cell of claim 32 wherein the DnaJ-like protein is selected from JEM1, MDJ2, SEC63, YDJ1, XDJ1, APJ1, SIS1, DJP1, ZUO1, SWA2, JJJ1, JJJ2, JJJ3, CAJ1, CWC23, PAM18, JAC1, JID1, SCJ1, HLJ1 and ERJ5."],"number":39,"annotation":false,"claim":true,"title":false},{"lines":["The host cell of claim 32 wherein the host cell is further genetically modified to cause over-expression of at least one, two, three, four, five, six or seven proteins involved in the formation of disulphide bonds in other proteins selected from the group consisting of ERO1, ERV2, EUG1, MPD1, MPD2, EPS1 and PDI1."],"number":40,"annotation":false,"claim":true,"title":false},{"lines":["The host cell that is suitable for enhanced production of a protein product of choice characterised in that the host cell comprises a first gene encoding a first helper protein selected from JEM1, LHS1 or SIL1 or a variant thereof, and a second gene encoding a desired protein product of choice, wherein the host cell is genetically modified to cause over-expression of the first helper protein, and\n
(a) wherein the first and second genes are not both present within the host cell on the same 2 μm-family plasmid; and\n
(b) wherein the host cell is not genetically modified to cause over-expression of a further helper protein that is different from the first helper protein and is selected from the group consisting of AHA1, CCT2, CCT3, CCT4, CCT5, CCT6, CCT7, CCT8, CNS1, CPR3, CPR6, ERO1, EUG1, FMO1, HCH1, HSP10, HSP12, HSP104, HSP26, HSP30, HSP42, HSP60, HSP78, HSP82, JEM1, MDJ1, MDJ2, MPD1, MPD2, PDI1, PFD1, ABC1, APJ1, ATP11, ATP12, BTT1, CDC37, CPR7, HSC82, KAR2, LHS1, MGE1, MRS11, NOB1, ECM10, SSA1, SSA2, SSA3, SSA4, SSC1, SSE2, SIL1, SLS1, ORM1, ORM2, PER1, PTC2, PSE1, UBI4 and HAC1 or a truncated intronless HAC1."],"number":41,"annotation":false,"claim":true,"title":false},{"lines":["The host cell of claim 41, wherein the first helper protein is JEM1, LHS1 or SIL1."],"number":42,"annotation":false,"claim":true,"title":false},{"lines":["The host cell of claim 42 wherein the first helper protein is the only helper protein that is over-expressed by the host cell."],"number":43,"annotation":false,"claim":true,"title":false},{"lines":["The host cell of claim 32 wherein the protein product of choice is a heterologous protein and/or comprises a leader sequence effective to cause secretion."],"number":44,"annotation":false,"claim":true,"title":false},{"lines":["The host cell of claim 32 wherein the protein product of choice is a eukaryotic protein, or a fragment or variant thereof."],"number":45,"annotation":false,"claim":true,"title":false},{"lines":["The host cell of claim 32 wherein the protein product of choice comprises albumin, a monoclonal antibody, an etoposide, a serum protein, antistasin, a tick anticoagulant peptide, transferrin, lactoferrin, endostatin, angiostatin, collagens, immunoglobulins, or immunoglobulin-based molecules or fragment of either, a Kunitz domain protein, interferons, interleukins, leptin, CNTF and fragments thereof, IL1-receptor antagonist, erythropoietin (EPO) and EPO mimics, thrombopoietin (TPO) and TPO mimics, prosaptide, cyanovirin-N, 5-helix, T20 peptide, T1249 peptide, HIV gp41, HIV gp120, urokinase, prourokinase, tPA, hirudin, platelet derived growth factor, parathyroid hormone, proinsulin, insulin, glucagon, glucagon-like peptides, insulin-like growth factor, calcitonin, growth hormone, transforming growth factor beta, tumour necrosis factor, G-CSF, GM-CSF, M-CSF, FGF, coagulation factors in both pre and active forms, including but not limited to plasminogen, fibrinogen, thrombin, pre-thrombin, pro-thrombin, von Willebrand's factor, alpha,-antitrypsin, plasminogen activators, Factor VII, Factor VIII, Factor IX, Factor X and Factor XIII, nerve growth factor, LACI, platelet-derived endothelial cell growth factor (PD-ECGF), glucose oxidase, serum cholinesterase, inter-alpha trypsin inhibitor, antithrombin III, apo-lipoprotein species, Protein C, Protein S, or a variant or fragment of any of the above, or a fusion of albumin and any of the above."],"number":46,"annotation":false,"claim":true,"title":false},{"lines":["The host cell of claim 32 wherein the protein product of choice comprises the sequence of albumin or a variant or fragment thereof."],"number":47,"annotation":false,"claim":true,"title":false},{"lines":["The host cell of claim 32 wherein the protein product of choice comprises the sequence of transferrin family member, or a variant or fragment thereof."],"number":48,"annotation":false,"claim":true,"title":false},{"lines":["The host cell of claim 32 wherein the protein product of choice comprises a fusion protein."],"number":49,"annotation":false,"claim":true,"title":false},{"lines":["The host cell of claim 32 comprising an exogenous polynucleotide sequence that encodes the protein product of choice."],"number":50,"annotation":false,"claim":true,"title":false},{"lines":["The host cell of claim 50 wherein the exogenous polynucleotide is integrated into the chromosome of the host cell."],"number":51,"annotation":false,"claim":true,"title":false},{"lines":["The host cell of claim 50 wherein the exogenous polynucleotide is present in the host cell as part of a replicable vector."],"number":52,"annotation":false,"claim":true,"title":false},{"lines":["A method for producing a protein product of choice, the method comprising:\n
(a) providing the host cell of claim 50; and\n
(b) growing the host cell;\nthereby to produce a cell culture or recombinant organism comprising an increased level of the protein product of choice compared to the level of production of the protein product of choice achieved by growing, under the same conditions, the same host cell that has not been genetically modified to cause over-expression of one or more helper proteins."],"number":53,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 53 wherein the step of growing the host cell involves culturing the host cell in a culture medium."],"number":54,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 53 further comprising the step of purifying the thus expressed protein product of choice from the cultured host cell, recombinant organism or culture medium."],"number":55,"annotation":false,"claim":true,"title":false},{"lines":["Method of preparing a the host cell of claim 32, by transformation of a host cell with a polynucleotide, wherein the polynucleotide comprises a sequence encoding a helper protein selected from the list comprising\n
(a) a chaperone selected from a DnaJ-like protein an Hsp70 family protein, and SIL1, and wherein the DnaJ-like protein is not SCJ1; and\n
(b) a protein involved in the formation of disulphide bonds in other proteins selected from ERO1, ERV2, EUG1, MPD1, MPD2, EPS1 and PDI1."],"number":56,"annotation":false,"claim":true,"title":false}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}