{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"US_2010_0261196_A1","frontPageModel":{"patentViewModel":{"ref":{"entityRefId":"163-908-157-172-646","entityRefType":"PATENT"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":8906,"type":"PATENT","title":"Univ Pennsylvania Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":24238,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8221,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search applicants and owners= \"Univ Pennsylvania\", \"Pennsylvania Univ\", \"Univ Penns*\", \"Penn* Univ\", \"University of Pennsylvania\", \"Pennsylvania University\".
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Search applicants and owners= \"Univ Pennsylvania\", \"Pennsylvania Univ\", \"Univ Penns*\", \"Penn* Univ\", \"University of Pennsylvania\", \"Pennsylvania University\".
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(a) labeling a cell fraction isolated from a transgenic non-human animal having a tamoxifen-dependent Cre-recombinase in the isl1 locus (isl1-mER-Cre-mER) and an Cre reporter (R16R) with a fluorescent label, wherein the cell fraction contains detectably labeled cardiac progenitor cells;\n
(b) identifying a test molecule from the cell fraction having increased fluorescence as compared to a control molecule;\n
(c) contacting the test molecule with the detectably labeled cardiac progenitor cells; and\n
(d) determining the expression of the isl1+ transcription factor,\nthereby identifying a small molecule affecting cardiogenesis."],"number":1,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein the non-human animal is a rat or mouse."],"number":2,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, further comprising detecting expression of one or more growth factors selected from the group consisting of BMP4, BMP7, and FGF10."],"number":3,"annotation":false,"claim":true,"title":false},{"lines":["A method of identifying a small molecule which regulates cardiogenesis comprising:\n
(a) labeling a cell fraction isolated from a transgenic non-human animal having a tamoxifen-dependent Cre-recombinase in the isl1 locus (isl1-mER-Cre-mER) and an Cre reporter with a fluorescent label, wherein the cell fraction contains detectably labeled cardiac progenitor cells;\n
(b) identifying a test molecule from the cell fraction having increased fluorescence as compared to a control molecule;\n
(c) contacting the test molecule with the detectably labeled cardiac progenitor cells;\n
(d) determining the expression of the isl1+ transcription factor; and\n
(e) identifying a factor which is modulates isl1+ expression,\nthereby identifying a small molecule which regulates cardiogenesis."],"number":4,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 4, wherein the non-human animal is a rat or mouse."],"number":5,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 4, further comprising detecting expression of one or more growth factors selected from the group consisting of BMP4, BMP7, and FGF10."],"number":6,"annotation":false,"claim":true,"title":false},{"lines":["A method for stimulating maturation of cardiac progenitor cells comprising contacting the cells with an effective amount of a GSK-3β inhibitor, thereby stimulating maturation of cardiac progenitor cells."],"number":7,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 7, wherein the inhibitor is 6-bromoindirubin-3′-oxime (BIO)."],"number":8,"annotation":false,"claim":true,"title":false},{"lines":["A method for generating an Isl1 lineage-traced cell comprising contacting an undifferentiated progenitor cell that expresses Isl1 with a GSK-3β inhibitor that activates or enhances expression of Isl1 in the cell, thereby generating a Isl1 lineage-traced cell."],"number":9,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 9, wherein the inhibitor is 6-bromoindirubin-3′-oxime (B10)."],"number":10,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 9, wherein the activation or enhancement of Isl1 expression causes the cell to differentiate into a cell selected from the group consisting of cardiomyocyte, endothelial cell, and smooth muscle cell."],"number":11,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 9, wherein the undifferentiated progenitor cell is selected from the group consisting of embryonic heart muscle cell, post-natal heart muscle cells, embryonic stem cells, progenitors from cord blood, and adult progenitor cells."],"number":12,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 12, wherein the adult progenitor cells are bone marrow cells or adipose tissue cells."],"number":13,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 12, wherein the cardiac cell is derived from a rat, mouse or human."],"number":14,"annotation":false,"claim":true,"title":false}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}