{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"US_2009_0217420_A1","frontPageModel":{"patentViewModel":{"ref":{"entityRefType":"PATENT","entityRefId":"161-991-156-914-545"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":11648,"type":"PATENT","title":"Tohoku University - Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":12827,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8314,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
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transforming a host cell with a recombination vector containing a gene of interest and a gene coding for a mutant acetolactate synthase having mutation of glycine corresponds to position 95 of the amino acid sequence of a wild-type acetolactate synthase derived from rice to alanine;\n
culturing the transformed cell obtained in the former step in the presence of a pyrimidinyl carboxy herbicide, and\n
wherein the gene coding for the mutant acetolactate synthase is used as a selection marker."],"number":1,"annotation":false,"title":false,"claim":true},{"lines":["The transformation method according to claim 1, wherein the gene coding for the mutant acetolactate synthase is a gene coding for the following protein (a) or (b):\n
(a) a protein comprising the amino acid sequence of SEQ ID NO: 2; or\n
(b) a protein comprising an amino acid sequence derived from the amino acid sequence of SEQ ID NO: 2 by substitution, deletion, or addition of at least one amino acid other than alanine of position 95, having resistance to a pyrimidinyl carboxy herbicide, and having acetolactate synthase activity."],"number":2,"annotation":false,"title":false,"claim":true},{"lines":["The transformation method according to claim 1, wherein the host cell is a plant cell."],"number":3,"annotation":false,"title":false,"claim":true},{"lines":["A method for cultivating a plant, comprising the steps of:\n
transforming a plant cell with a recombination vector containing a gene of interest and a gene coding for a mutant acetolactate synthase having mutation of glycine corresponds to position 95 of the amino acid sequence of a wild-type acetolactate synthase derived from rice to alanine;\n
cultivating the transformed plant obtained in the former step in the presence of a pyrimidinyl carboxy herbicide, and\n
wherein the gene coding for the mutant acetolactate synthase is used as a selection marker."],"number":4,"annotation":false,"title":false,"claim":true},{"lines":["The method for cultivating a plant according to claim 4, wherein the gene coding for the mutant acetolactate synthase is a gene coding for the following protein (a) or (b):\n
(a) a protein comprising the amino acid sequence of SEQ ID NO: 2; or\n
(b) a protein comprising an amino acid sequence derived from the amino acid sequence of SEQ ID NO: 2 by substitution, deletion, or addition of at least one amino acid other than alanine of position 95, having resistance to a pyrimidinyl carboxy herbicide, and having acetolactate synthase activity."],"number":5,"annotation":false,"title":false,"claim":true}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}