In Situ Cloning From Pathological Tissue Specimens


The present invention pertains to methods related to cloning nucleic acids from biological samples, particularly pathological tissue samples. This method includes hybridizing a population of oligonucleotide sequence probes comprising degenerate sequence tags to a fixed tissue, isolating the hybridized oligonucleotide sequence probes and amplifying the sequence tags in the hybridized oligonucleotide sequence probes. This method can be utilized to identify genes associated with disease and to quantitate the expression of disease-related transcripts. The method can also be used to identify truncated mRNAs.

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Document History
  • Publication: Jan 22, 2009
  • Application: Apr 9, 2007
    US US 78467707 A
  • Priority: Apr 9, 2007
    US US 78467707 A
  • Priority: Apr 7, 2006
    US US 79038206 P

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