Novel Method For Screening Bacterial Transcription Modulators

Abstract

A method for detecting a compound modulating complexing between RNA polymerase and a protein intervening during transcription, which consists in: incubating a mixture comprising RNA polymerase intervening during transcription and the compound to be detected; detecting, by a complexing test, the possible significant variation of the amount of complex formed between RNA polymerase and the protein relative to a control value corresponding to the amount of complex formed between RNA polymerase and the protein in the absence of any modulator; and deducing therefrom, when there is a significant variation as defined above, that there has been formation of a bond between the compound and RNA polymerase and/or the protein intervening during transcription, which results in a modulation of the complexing between RNA polymerase and the protein intervening during transcription.


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Document History
  • Publication: Mar 11, 2004
  • Application: Sep 10, 2003
    US US 43298703 A
  • Priority: Nov 27, 2001
    FR FR 0103749 W
  • Priority: Nov 28, 2000
    FR FR 0015332 A

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