Novel Method For Rapid And Efficient Generation Of Neuroectoderm Cells And Peripheral Neurons From Pluripotent Stem Cells

  • Published: Sep 12, 2012
  • Earliest Priority: Mar 07 2011
  • Family: 1
  • Cited Works: 89
  • Cited by: 2
  • Cites: 10
  • Additional Info: Cited Works Published
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Abstract

The present invention relates to a method for generating neuroectoderm cells, the method comprising: culturing pluripotent cells for about 2 to about 6 days, wherein the expression and/or activity of one or more proteins selected from the group consisting of fibroblast growth factor receptor (FGFR), son of sevenless (SOS), growth factor receptor-bound protein 2 (GRB2), rat sarcoma protein (RAS), rat fibrosarcoma (RAF), mitogen-activated protein kinase kinase (MEK) and mitogen-activated protein kinase (MAPK) is inhibited. The present invention further relate to a method for generating neurons, the method comprising the steps for generating neuroectoderm cells in accordance with the invention and culturing the neuroectoderm cells for at least about 2 further days. Furthermore, the present invention also relates to a kit comprising means for inhibiting the expression and/or activity of one or more of the proteins selected from the group consisting of FGFR, SOS, GRB2, RAS, RAF, MEK and MAPK, and/or means inhibiting the nodal/activin/TGFbeta/SMAD2/3 signalling pathway, and/or the BMP/GDF/SMAD1/5/8 signalling pathway and, optionally, pluripotent cells.


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Document History
  • Publication: Sep 12, 2012
  • Application: Mar 7, 2011
    EP EP 11001870 A
  • Priority: Mar 7, 2011
    EP EP 11001870 A

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