{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"EP_2415872_A1","frontPageModel":{"patentViewModel":{"ref":{"entityRefType":"PATENT","entityRefId":"127-125-300-490-262"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":11813,"type":"PATENT","title":"University of Aberdeen Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":27926,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8443,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately: univ* aberdeen. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 1658
Search Applicants and Owners separately: univ* aberdeen. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 1658
(i) the zinc finger protein is engineered to bind to a target sequence;
(ii) (XD)1-10 comprises an amino acid sequence selected from the group consisting of QKP, QLV, and a G residue immediately C-terminal to the C-terminal Cys residue; and
(iii) when (XD)1-10 comprises the amino acid sequence QKP and (XC)3-5 consists of 3 amino acids, the residues of (XC)3 are selected from the group consisting of IRT, IRR, TKI and AQR."],"number":1,"annotation":false,"title":false,"claim":true},{"lines":["The zinc finger protein of claim 1, comprising any of the sequences shown in any of Tables 1, 2, 3 or 4."],"number":2,"annotation":false,"title":false,"claim":true},{"lines":["The zinc finger protein of claim 1 or claim 2, wherein XD comprises the sequence QLV, QKP, G, GG or GGG."],"number":3,"annotation":false,"title":false,"claim":true},{"lines":["A zinc finger protein comprising a plurality of zinc fingers, wherein at least one of the zinc fingers comprises a CCHC zinc finger according to any of claims 1 to 3."],"number":4,"annotation":false,"title":false,"claim":true},{"lines":["The zinc finger protein of any of claims 1 to 4, wherein the zinc finger protein comprises any of the sequences shown in Table 8 and is engineered to bind to a target sequence in an IPP2-K gene."],"number":5,"annotation":false,"title":false,"claim":true},{"lines":["A fusion protein comprising a zinc finger protein of any of claims 1 to 5 and one or more functional domains."],"number":6,"annotation":false,"title":false,"claim":true},{"lines":["The fusion protein of claim 6, wherein the functional domain comprises a cleavage half-domain."],"number":7,"annotation":false,"title":false,"claim":true},{"lines":["The fusion protein of claim 7, further comprising a ZC linker between the cleavage half-domain and the zinc finger protein."],"number":8,"annotation":false,"title":false,"claim":true},{"lines":["A polynucleotide encoding at least one zinc finger protein or fusion protein according to any of claims 1 to 8."],"number":9,"annotation":false,"title":false,"claim":true},{"lines":["A plant cell comprising at least one zinc finger protein, at least one fusion protein or at least one polynucleotide according to any of claims 1 to 9."],"number":10,"annotation":false,"title":false,"claim":true},{"lines":["The plant cell of claim 10, wherein the cell is a seed."],"number":11,"annotation":false,"title":false,"claim":true},{"lines":["The plant cell of claim 10 to 11, wherein IPP2-K is partially or fully inactivated and levels of phytic acid in the seed are reduced."],"number":12,"annotation":false,"title":false,"claim":true},{"lines":["A method for targeted cleavage of cellular chromatin in a plant cell, the method comprising expressing, in the cell, a pair of fusion proteins according to claim 7 or at least one polynucleotide encoding the pair of fusion proteins; wherein:
(a) the target sequences of the fusion proteins are within ten nucleotides of each other; and
(b) the fusion proteins dimerize and cleave DNA located between the target sequences."],"number":13,"annotation":false,"title":false,"claim":true},{"lines":["A method of targeted genetic recombination in a plant cell, the method comprising:
(a) expressing, in the host cell, a pair of fusion proteins according to claim 7 or at least one polynucleotide encoding the pair of fusion proteins, wherein the target sequences of the fusion proteins are present in a chosen host target locus; and
(b) identifying a recombinant host cell which exhibits a sequence alteration in the host target locus and
(c) optionally introducing an exogenous polynucleotide into the host cell, wherein, when present, the exogenous polynucleotide is integrated into the genome of the host plant cell."],"number":14,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 14, wherein the sequence alteration is a mutation selected from the group consisting of a deletion of genetic material, an insertion of genetic material, a substitution of genetic material and any combination thereof."],"number":15,"annotation":false,"title":false,"claim":true},{"lines":["A method for reducing the level of phytic acid in a plant seed or making phosphorous more metabolically available in a plant seed, the method comprising inactivating or altering an IPP2-K gene according to claim 14."],"number":16,"annotation":false,"title":false,"claim":true}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}