{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"EP_2412812_A1","frontPageModel":{"patentViewModel":{"ref":{"entityRefId":"163-084-029-537-779","entityRefType":"PATENT"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":11813,"type":"PATENT","title":"University of Aberdeen Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":27926,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8443,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately: univ* aberdeen. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 1658
Search Applicants and Owners separately: univ* aberdeen. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 1658
(a) a cleavage half-domain,
(b) the zinc finger protein of any of claims 1 to 13, and
(c) a ZC linker interposed between the cleavage half-domain and the zinc finger protein."],"number":15,"annotation":false,"claim":true,"title":false},{"lines":["The fusion protein of claim 15, wherein the length of the ZC linker is 5 amino acids."],"number":16,"annotation":false,"claim":true,"title":false},{"lines":["The fusion protein of claim 16, wherein the amino acid sequence of the ZC linker is GLRGS (SEQ ID NO:4)."],"number":17,"annotation":false,"claim":true,"title":false},{"lines":["The fusion protein of claim 15, wherein the length of the ZC linker is 6 amino acids."],"number":18,"annotation":false,"claim":true,"title":false},{"lines":["The fusion protein of claim 18, wherein the amino acid sequence of the ZC linker is GGLRGS (SEQ ID NO:5)."],"number":19,"annotation":false,"claim":true,"title":false},{"lines":["A polynucleotide encoding a zinc finger protein according to any of claims 1 to 13 or a fusion protein according to any of claims 14 to 19."],"number":20,"annotation":false,"claim":true,"title":false},{"lines":["A method for targeted cleavage of cellular chromatin in a plant cell, the method comprising expressing, in the cell, a pair of fusion proteins according to any of claims 15 to 19; wherein:
(a) the target sequences of the fusion proteins are within ten nucleotides of each other; and
(b) the fusion proteins dimerize and cleave DNA located between the target sequences."],"number":21,"annotation":false,"claim":true,"title":false},{"lines":["A method of targeted genetic recombination in a host plant cell, the method comprising:
(a) expressing, in the host cell, a pair of fusion proteins according to any of claims 15 to 19, wherein the target sequences of the fusion proteins are present in a chosen host target locus; and
(b) identifying a recombinant host cell which exhibits a sequence alteration in the host target locus."],"number":22,"annotation":false,"claim":true,"title":false},{"lines":["The method of either of claim 21 or claim 22, wherein the sequence alteration is a mutation selected from the group consisting of a deletion of genetic material, an insertion of genetic material, a substitution of genetic material and any combination thereof."],"number":23,"annotation":false,"claim":true,"title":false},{"lines":["The method of any of claims 21 to 23, further comprising introducing an exogenous polynucleotide into the host cell."],"number":24,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 24, wherein the exogenous polynucleotide comprises sequences homologous to the host target locus."],"number":25,"annotation":false,"claim":true,"title":false},{"lines":["The method of any of claims 21 to 25 wherein the plant is selected from the group consisting of a monocotyledon, a dicotyledon, gymnosperms and eukaryotic algae."],"number":26,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 26, wherein the plant is selected from the group consisting of maize, rice, wheat, potato, soybean, tomato, tobacco, members of the Brassica family, and Arabidopsis."],"number":27,"annotation":false,"claim":true,"title":false},{"lines":["The method of any of claims 21 to 26 wherein the plant is a tree."],"number":28,"annotation":false,"claim":true,"title":false},{"lines":["The method of any of claims 21 to 28, wherein the target sequences are in an IPP2K gene."],"number":29,"annotation":false,"claim":true,"title":false},{"lines":["A method for reducing the level of phytic acid in seeds, the method comprising inactivating or altering an IPP2-K gene according to claim 29."],"number":30,"annotation":false,"claim":true,"title":false},{"lines":["A method for making phosphorous more metabolically available in seed, the method comprising inactivating or altering an IPP2-K gene according to claim 29."],"number":31,"annotation":false,"claim":true,"title":false},{"lines":["A plant cell comprising a zinc finger protein according to any of claims 1 to 13, a fusion protein according to any of claims 14 to 19 or a polynucleotide according to claim 20."],"number":32,"annotation":false,"claim":true,"title":false},{"lines":["The plant cell of claim 32, wherein the cell is a seed."],"number":33,"annotation":false,"claim":true,"title":false},{"lines":["The plant cell of claim 33, wherein seed is a corn seed."],"number":34,"annotation":false,"claim":true,"title":false},{"lines":["The plant cell of any of claims 32 to 34, wherein IPP2-K is partially or fully inactivated."],"number":35,"annotation":false,"claim":true,"title":false}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}