Methods For Producing Members Of Specific Binding Pairs

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Abstract

A member of a specific binding pair (sbp) is identified by expressing DNA encoding a genetically diverse population of such sbp members in recombinant host cells in which the sbp members are displayed in functional form at the surface of a secreted recombinant genetic display package (rgdp) containing DNA encoding the sbp member or a polypeptide component thereof, by virtue of the sbp member or a polypeptide component thereof being expressed as a fusion with a capsid component of the rgdp. The displayed sbps may be selected by affinity with a complementary sbp member, and the DNA recovered from selected rgdps for expression of the selected sbp members. Antibody sbp members may be thus obtained, with the different chains thereof expressed, one fused to the capsid component and the other in free form for association with the fusion partner polypeptide. A phagemid may be used as an expression vector, with said capsid fusion helping to package the phagemid DNA. Using this method libraries of DNA encoding respective chains of such multimeric sbp members may be combined, thereby obtaining a much greater genetic diversity in the sbp members than could easily be obtained by conventional methods.


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Document History
  • Publication: May 6, 2009
  • Application: Jul 10, 1991
    EP EP 09001901 A
  • Priority: Jul 10, 1991
    EP EP 06018386 A
  • Priority: Jul 10, 1991
    EP EP 97120149 A
  • Priority: Jul 10, 1991
    EP EP 96112510 A
  • Priority: Jul 10, 1991
    EP EP 91913039 A
  • Priority: May 15, 1991
    GB GB 9110549 A
  • Priority: Mar 6, 1991
    GB GB 9104744 A
  • Priority: Nov 12, 1990
    GB GB 9024503 A
  • Priority: Oct 19, 1990
    GB GB 9022845 A
  • Priority: Jul 10, 1990
    GB GB 9015198 A

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