Linkers And Co-coupling Agents For Optimization Of Oligonucleotide Synthesis And Purification On Solid Support

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Abstract

A method of modulation of synthesis capacity on and cleavage properties of synthetic oligomers from solid support is described. The method utilizes linker molecules attached to a solid surface and co-coupling agents that have similar reactivities to the coupling compounds with the surface functional groups. The preferred linker molecules provide an increased density of polymers and more resistance to cleavage from the support surface. The method is particularly useful for synthesis of oligonucleotides, oligonucleotides microarrays, peptides, and peptide microarrays. The stable linkers are also coupled to anchor molecules for synthesis of DNA oligonucleotides using on support purification, eliminating time-consuming chromatography and metal cation presence. Oligonucleotides thus obtainded can be directly used for mass analysis, DNA amplification and ligation, hybridization, and many other applications.


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Document History
  • Publication: Apr 15, 2009
  • Application: Mar 14, 2002
    EP EP 08019497 A
  • Priority: Mar 14, 2002
    EP EP 02733860 A
  • Priority: Mar 13, 2002
    US US 9938202 A
  • Priority: Mar 14, 2001
    US US 27566601 P

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