Isolated Human Cell, Method For Obtaining The Same And Their Use

  • Published: Jul 30, 2008
  • Family: 9
  • Cited scholarly works: 0
  • Cited by: 1
  • Cites: 0
  • Additional Info: Published
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Abstract

A novel cell suited for mass production of Hemagglutinating Virus of Japan (HVJ), a method for obtaining the cell and use of the cell are disclosed. The human cell is originated from a transformed human kidney cell line, the doubling time thereof in logarithmic growth phase in suspension culture in a serum-free medium is not more than 40 hours, the cell has a freeze-recovery property, the maximum density of viable cells in suspension culture is not less than 10 6 cells/mL, and HVJ can grow in the cell. The method for obtaining the human cell comprises the steps of suspension-culturing a human transformed kidney cell line in a serum-free medium, and cloning the grown cells; and selecting, from the cloned cells, a cell whose doubling time in logarithmic growth phase in suspension culture in a serum-free medium is not more than 40 hours, which has a freeze-recovery property, whose maximum density of viable cells in suspension culture is not less than 10 6 cells/mL, in which HVJ can grow.


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Document History
  • Publication: Jul 30, 2008
  • Application: Oct 4, 2006
    EP EP 06811189 A
  • Priority: Oct 4, 2006
    JP 2006319849 W
  • Priority: Oct 5, 2005
    JP 2005292045 A

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