{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"EP_1939294_A1","frontPageModel":{"patentViewModel":{"ref":{"entityRefType":"PATENT","entityRefId":"085-039-735-024-188"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":8877,"type":"PATENT","title":"New York Univ Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":15466,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8211,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners seperately = \"New York Univ\", \"New York University NOT \"state\" NOT \"city\" \", \"Univ New York\", \"NYU\".
Search Applicants and Owners seperately = \"New York Univ\", \"New York University NOT \"state\" NOT \"city\" \", \"Univ New York\", \"NYU\".
(a) the OspA and OspC polypeptides are from the same strain of Borrelia burgdorferi; and/or
(b) the OspA and OspC polypeptides are from the same genospecies of Borrelia burgdorferi; and/or
(c) the OspA and OspC polypeptides are from different strains of Borrelia burgdorferi; and/or
(d) the OspA and OspC polypeptides are from different genospecies of Borrelia burgdorferi; and/or
(e) the chimeric OspA polypeptide comprises OspA polypeptides from different genospecies of Borrelia burgdorferi."],"number":3,"annotation":false,"title":false,"claim":true},{"lines":["The chimeric protein of any one of Claims 1-3, wherein the OspA polypeptide comprises
(a) a Borrelia burgdorferi OspA polypeptide from amino acid residue 18 to amino acid residue 216 of a first strain of Borrelia burgdorferi, and from amino acid residue 217 to amino acid residue 273 of a second strain of Borrelia burgdorferi; or
(b) a Borrelia burgdorferi OspA polypeptide from amino acid residue 132 to amino acid residue 217 of a first strain of Borrelia burgdorferi, and a Borrelia burgdorferi OspA polypeptide from amino acid residue 218 to amino acid residue 273 of a second strain of Borrelia burgdorferi,wherein the amino acid numbering is based on the OspA polypeptide sequence of SEQ ID NO: 7."],"number":4,"annotation":false,"title":false,"claim":true},{"lines":["The chimeric protein of any one of Claims 1-4, wherein the OspA polypeptide comprises at least four separate OspA polypeptide fragments; and further optionally wherein the first OspA polypeptide fragment comprises a Borrelia burgdorferi OspA polypeptide from amino acid residue 30 to amino acid residue 150, wherein the second OspA polypeptide fragment comprises a Borrelia burgdorferi OspA polypeptide from amino acid residue 151 to amino acid residue 179, wherein the third OspA polypeptide fragment comprises a Borrelia burgdorferi OspA polypeptide from amino acid residue 180 to amino acid residue 216, and wherein the fourth OspA polypeptide fragment comprises a Borrelia burgdorferi OspA polypeptide from amino acid residue 217 to amino acid residue 273, wherein the amino acid numbering is based on the OspA polypeptide sequence of SEQ ID NO: 7."],"number":5,"annotation":false,"title":false,"claim":true},{"lines":["A chimeric protein comprising an amino acid sequence selected from the group consisting of: SEQ ID NOs: 146, 148, 150, 152, 154, 156, 158, 160, 162, 164, 166, 168, 170, 172, 174, 176, 178 and 180."],"number":6,"annotation":false,"title":false,"claim":true},{"lines":["A nucleic acid encoding an unlipidated chimeric protein comprising:
at least a first and a second polypeptide, wherein the first polypeptide comprises Borrelia burgdorferi OspC and wherein the second polypeptide comprises Borrelia burgdorferi OspA, such that OspC comprises the N-terminus of the encoded protein, wherein the OspC polypeptide is selected from the group consisting of: amino acid residue 19 to amino acid residue 211, amino acid residue 19 to 204, and amino acid residue 19 to 213, wherein the amino acid numbering is based on the OspC polypeptide of SEQ ID NO: 30, or
the OspA polypeptide is selected from the group consisting of: amino acid residue 18 to 273, and amino acid residue 132 to 273, wherein the amino acid numbering is based on the OspA polypeptide of SEQ ID NO: 7, or
the OspA polypeptide is a chimeric polypeptide comprising OspA polypeptides from at least two different strains of Borrelia burgdorferi."],"number":7,"annotation":false,"title":false,"claim":true},{"lines":["A nucleic acid encoding an unlipidated chimeric protein consisting of an OspC polypeptide and an OspA polypeptide from Borrelia burgdorferi, wherein the OspC polypeptide is linked to the N-terminus of the OspA polypeptide."],"number":8,"annotation":false,"title":false,"claim":true},{"lines":["The nucleic acid of Claim 8, wherein the OspA polypeptide comprises amino acid residue 18 to amino acid residue 216 of a first strain of Borrelia burgdorferi and from amino acid residue 217 to amino acid residue 273 of a second strain of Borrelia burgdorferi, or from amino acid residue 132 to amino acid residue 216 of a first strain of Borrelia burgdorferi and amino acid residue 217 to amino acid residue 273 of a second strain of Borrelia burgdorferi, wherein the amino acid numbering is based on the OspA polypeptide sequence of SEQ ID NO: 7."],"number":9,"annotation":false,"title":false,"claim":true},{"lines":["The nucleic acid of Claim 8 or 9, wherein the OspA polypeptide comprises at least four separate OspA polypeptide fragments, wherein the first OspA polypeptide fragment comprises a Borrelia burgdorferi OspA polypeptide from amino acid residue 30 to amino acid residue 150, wherein the second OspA polypeptide comprises a Borrelia burgdorferi OspA polypeptide from amino acid residue 151 to amino acid residue 179, wherein the third OspA polypeptide comprises a Borrelia burgdorferi OspA polypeptide from amino acid residue 181 to amino acid residue 216, and wherein the fourth OspA polypeptide comprises a Borrelia burgdorferi OspA polypeptide from amino acid residue 216 to amino acid residue 273, wherein the amino acid numbering is based on the OspA polypeptide sequence of SEQ ID NO: 7."],"number":10,"annotation":false,"title":false,"claim":true},{"lines":["A nucleic acid of any one of Claims 8-10, wherein:
(a) the encoded OspC and OspA polypeptides are from different strains of Borrelia burgdorferi and/or the encoded OspC and OspA polypeptides are from different genospecies of Borrelia burgdorferi; and/or
(b) the encoded OspC and OspA polypeptides are from the same strains of Borrelia burgdorferi; and/or
(c) the encoded OspC and OspA polypeptides are from the same genospecies of Borrelia burgdorferi."],"number":11,"annotation":false,"title":false,"claim":true},{"lines":["A nucleic acid selected from the group consisting of: SEQ ID NOs: 145, 147, 149, 151, 153, 155, 157, 159, 161, 163, 165, 167, 169, 171, 173, 175, 177 and 179."],"number":12,"annotation":false,"title":false,"claim":true},{"lines":["An expression vector comprising either:
(a) an isolated DNA having a nucleotide sequence selected from the group consisting of: SEQ ID NOs: 145, 147, 149, 151, 153, 155, 157, 159, 161, 163, 165, 167, 169, 171, 173, 175, 177 and 179; or
(b) an isolated DNA encoding a polypeptide having an amino acid sequence selected from the group consisting of: SEQ ID NOs: 146, 148, 150, 152, 154, 156, 158, 160, 162, 164, 166, 168, 170, 172, 174, 176, 178 and 180."],"number":13,"annotation":false,"title":false,"claim":true},{"lines":["A host cell comprising a recombinant nucleic acid either:
(a) having a nucleotide sequence selected from the group consisting of: SEQ ID NOs: 145, 147, 149, 151, 153, 155, 157, 159, 161, 163, 165, 167, 169, 171, 173, 175, 177 and 179; or
(b) encoding a protein having an amino acid sequence selected from the group consisting of: SEQ ID NOs: 146, 148, 150, 152, 154, 156, 158, 160, 162, 164, 166, 168, 170, 172, 174, 176, 178 and 180."],"number":14,"annotation":false,"title":false,"claim":true},{"lines":["A method of producing an unlipidated chimeric Borrelia burgdorferi protein comprising;
(a) selecting a polynucleotide encoding OspC or an antigenic portion thereof;
(b) selecting a polynucleotide encoding OspA or an antigenic portion thereof; and
(c) ligating the polynucleotide of a) to the polynucleotide of b), to form a chimeric polynucleotide sequence encoding a chimeric OspC-OspA protein such that OspC is upstream of OspA, wherein the OspC polypeptide is selected from the group consisting of: amino acid residue 19 to amino acid residue 211, amino acid residue 19 to 204, and amino acid residue 19 to 213, wherein the amino acid numbering is based on the OspC polypeptide of SEQ ID NO: 30, or the OspA polypeptide is selected from the group consisting of: amino acid residue 18 to 273, and amino acid residue 132 to 273, wherein the amino acid numbering is based on the OspA polypeptide of SEQ ID NO: 7, or the OspA polypeptide is a chimeric polypeptide comprising OspA polypeptides from at least two different strains of Borrelia burgdorferi."],"number":15,"annotation":false,"title":false,"claim":true},{"lines":["The method of Claim 15, wherein:
(a) the chimeric OspC-OspA protein has an amino acid sequence selected from the group consisting of: SEQ ID NOs: 146, 148, 150, 152, 154, 156, 158, 160, 162, 164, 166, 168, 170, 172, 174, 176, 178 and 180; and/or
(b) the method for producing a chimeric protein further comprising maintaining a host cell comprising the chimeric polynucleotide of Claim 17 under conditions suitable for expression of the polynucleotide."],"number":16,"annotation":false,"title":false,"claim":true},{"lines":["An unlipidated chimeric protein for use in immunizing an animal against Lyme's Disease, comprising at least a first and a second polypeptide, the first polypeptide comprises Borrelia burgdorferi OspC and wherein the second polypeptide comprises Borrelia burgdorferi OspA, such that OspC comprises the N-terminus of the protein, comprising administering the chimeric protein to an animal, wherein the OspC polypeptide is selected from the group consisting of: amino acid residue 19 to amino acid residue 211, amino acid residue 19 to 204, and amino acid residue 19 to 213, wherein the amino acid numbering is based on the OspC polypeptide of SEQ ID NO: 30, or the OspA polypeptide is selected from the group consisting of: amino acid residue 18 to 273, and amino acid residue 132 to 273, wherein the amino acid numbering is based on the OspA polypeptide of SEQ ID NO: 7, or the OspA polypeptide is a chimeric polypeptide comprising OspA polypeptides from at least two different strains of Borrelia burgdorferi."],"number":17,"annotation":false,"title":false,"claim":true},{"lines":["The chimeric protein of Claim 17, wherein:
(a) the chimeric protein is administered with a physiologically-acceptable vehicle or carrier;
(b) the chimeric protein is encoded by a nucleic acid selected from the group consisting of: SEQ ID NOs: 145, 147, 149, 151, 153, 155, 157, 159, 161, 163, 165, 167, 169, 171, 173, 175, 177 and 179; or
(c) the chimeric protein comprises an amino acid sequence selected from the group consisting of: SEQ ID NOs: 146, 148, 150, 152, 154, 156, 158, 160, 162, 164, 166, 168, 170, 172, 174, 176, 178 and 180."],"number":18,"annotation":false,"title":false,"claim":true},{"lines":["A method of detecting an unlipidated chimeric protein comprising at least a first and a second polypeptide, wherein the first polypeptide comprises Borrelia burgdorferi OspC and wherein the second polypeptide comprises Borrelia burgdorferi OspA, such that OspC comprises the N-terminus of the protein, comprising;
(a) contacting a host sample with the chimeric protein, under conditions wherein antibodies, if present, in the host sample bind to the chimeric protein forming antigen antibody complexes; and
(b) detecting the antigen antibody complexes, wherein the OspC polypeptide is selected from the group consisting of:"],"number":19,"annotation":false,"title":false,"claim":true},{"lines":["The method of Claim 19, wherein:amino acid residue 19 to amino acid residue 211, amino acid residue 19 to 204, and amino acid residue 19 to 213, wherein the amino acid numbering is based on the OspC polypeptide of SEQ ID NO: 30, orthe OspA polypeptide is selected from the group consisting of: amino acid residue 18 to 273, and amino acid residue 132 to 273, wherein the amino acid numbering is based on the OspA polypeptide of SEQ ID NO: 7, orthe OspA polypeptide is a chimeric polypeptide comprising OspA polypeptides from at least two different strains of Borrelia burgdorferi.
(a) the chimeric protein is encoded by a nucleic acid selected from the group consisting of: SEQ ID NOs: 145, 147, 149, 151, 153, 155, 157, 159, 161, 163, 165, 167, 169, 171, 173, 175, 177 and 179; or
(b) the chimeric protein comprises an amino acid sequence selected from the group consisting of: SEQ ID NOs: 146, 148, 150, 152, 154, 156, 158, 160, 162, 164, 166, 168, 170, 172, 174, 176, 178 and 180."],"number":20,"annotation":false,"title":false,"claim":true},{"lines":["A nucleic acid that encodes an unlipidated chimeric protein comprising a first and a second polypeptide, wherein the first polypeptide comprises Borrelia burgdorferi OspC and wherein the second polypeptide comprises Borrelia burgdorferi OspA, such that OspC comprises the N-terminus of the protein, for use in immunizing an animal against Lyme Disease, wherein the OspC polypeptide is selected from the group consisting of:
amino acid residue 19 to amino acid residue 211, amino acid residue 19 to 204, and amino acid residue 19 to 213, wherein the amino acid numbering is based on the OspC polypeptide of SEQ ID NO: 30, or
the OspA polypeptide is selected from the group consisting of: amino acid residue 18 to 273, and amino acid residue 132 to 273, wherein the amino acid numbering is based on the OspA polypeptide of SEQ ID NO: 7, or
the OspA polypeptide is a chimeric polypeptide comprising OspA polypeptides from at least two different strains of Borrelia burgdorferi and optionally
wherein the nucleic acid encoding the chimeric protein is selected from the group consisting of: SEQ ID NOs: 145, 147, 149, 151, 153, 155, 157, 159, 161, 163, 165, 167, 169, 171, 173, 175, 177 and 179."],"number":21,"annotation":false,"title":false,"claim":true},{"lines":["A physiological composition to vaccinate against and treat Borrelia infection in animals or humans, the composition comprising;
(a) either (i) an unlipidated chimeric protein comprising at least a first and a second polypeptide, wherein the first polypeptide comprises Borrelia burgdorferi OspC and wherein the second polypeptide comprises Borrelia burgdorferi OspA, such that OspC comprises the N-terminus of the protein, wherein the OspC polypeptide is selected from the group consisting of: amino acid residue 19 to amino acid residue 211, amino acid residue 19 to 204, and amino acid residue 19 to 213, wherein the amino acid numbering is based on the OspC polypeptide of SEQ ID NO: 30, or the OspA polypeptide is selected from the group consisting of: amino acid residue 18 to 273, and amino acid residue 132 to 273, wherein the amino acid numbering is based on the OspA polypeptide of SEQ ID NO: 7, or the OspA polypeptide is a chimeric polypeptide comprising OspA polypeptides from at least two different strains of Borrelia burgdorferi, the amount being effective to elicit an immun Type your question here and then click Searche response to Borrelia burgdorferi; or (ii) a nucleic acid encoding said chimeric protein;
(b) a physiologically-acceptable carrier or vehicle; and optionally
(c) an adjuvant."],"number":22,"annotation":false,"title":false,"claim":true},{"lines":["The composition of Claim 24, wherein either
(a) the chimeric protein is selected from the group consisting of: SEQ ID NOs: 146, 148, 150, 152, 154, 156, 158, 160, 162, 164, 166, 168, 170, 172, 174, 176, 178 and 180; and/or
(b) the nucleic acid is selected from the group consisting of: SEQ ID NOs: 145, 147, 149, 151, 153, 155, 157, 159, 161, 163, 165, 167, 169, 171, 173, 175, 177 and 179."],"number":23,"annotation":false,"title":false,"claim":true},{"lines":["A kit comprising;
(a) an unlipidated chimeric protein comprising at least a first and a second polypeptide, wherein the first polypeptide comprises Borrelia burgdorferi OspC and wherein the second polypeptide comprises Borrelia burgdorferi OspA, such that OspC comprises the N-terminus of the protein, wherein the OspC polypeptide is selected from the group consisting of: amino acid residue 19 to amino acid residue 211, amino acid residue 19 to 204, and amino acid residue 19 to 213, wherein the amino acid numbering is based on the OspC polypeptide of SEQ ID NO: 30, or the OspA polypeptide is selected from the group consisting of: amino acid residue 18 to 273, and amino acid residue 132 to 273, wherein the amino acid numbering is based on the OspA polypeptide of SEQ ID NO: 7, or the OspA polypeptide is a chimeric polypeptide comprising OspA polypeptides from at least two different strains of Borrelia burgdorferi, and
(b) reagents for detecting antibody antigen complexes formed between the chimeric protein and antibodies, if present, in a user-supplied host sample."],"number":24,"annotation":false,"title":false,"claim":true},{"lines":["The kit of Claim 24, wherein the chimeric protein is selected from the group consisting of: SEQ ID NOs: 146, 148, 150, 152, 154, 156, 158, 160, 162, 164, 166, 168, 170, 172, 174, 176, 178 and 180."],"number":25,"annotation":false,"title":false,"claim":true}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}