Methods For Purifying Highly Anionic Proteins

  • Published: Oct 25, 2006
  • Earliest Priority: Jun 05 2001
  • Family: 25
  • Cited Works: 1
  • Cited by: 0
  • Cites: 4
  • Sequences: 1
  • Additional Info: Published Sequence
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Abstract

The present invention is directed to a method for dissociating Fc containing molecules from complexes of Protein A/Fc containing molecules in a mixture comprising contacting the mixture with a metal chelate chromatographic column (IMAC) under pH conditions between 5.0 and 5.7, wherein the pH conditions are established by the addition of ethylene glycol to said mixture to dissociate Fc containing molecules from complexes of Protein A/Fc containing molecules.


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Document History
  • Publication: Oct 25, 2006
  • Application: Jun 5, 2002
    EP EP 06010184 A
  • Priority: Jun 5, 2002
    EP EP 02749564 A
  • Priority: Jun 5, 2001
    US US 29640201 P

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