{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"EP_1218530_B1","frontPageModel":{"patentViewModel":{"ref":{"entityRefType":"PATENT","entityRefId":"152-771-707-288-878"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":11706,"type":"PATENT","title":"The University of Georgia - Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":3798,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8360,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
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the organism is the organism Caloramator viterbiensis deposited as ATCC designation PTA-584, or the progeny thereof; and
wherein the organism is capable of fermenting glycerol to 1,3-propanediol."],"number":1,"annotation":false,"title":false,"claim":true},{"lines":["A method of converting glycerol to 1,3-propanediol in a thermophilic organism, the method comprising:
providing a thermophilic organism that ferments glycerol to 1,3-propanediol, wherein the thermophilic organism is the organism of claim 1; and
culturing the thermophilic organism under conditions such that 1,3-propanediol is produced."],"number":2,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 2, wherein the organism is cultured at a pH between 5.0 to 7.6."],"number":3,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 2, wherein the organism is cultured at a pH between 6.0 to 6.5."],"number":4,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 2, wherein the organism is cultured at a temperature between 33°C and 64 °C."],"number":5,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 2, wherein the organism is cultured at a pH of 6.0 and a temperature of 58°C."],"number":6,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 2, wherein the organism is cultured under conditions selected from the group consisting of: anaerobic conditions, under nitrogen, under argon, under a mixture of nitrogen and carbon dioxide in a ratio of nitrogen to carbon dioxide of about 80 to about 20, in the presence of an oxygen scavenger, in an anaerobic chamber, under microaerobic conditions, adsorbed on a solid support, and under aerobic conditions."],"number":7,"annotation":false,"title":false,"claim":true},{"lines":["An isolated enzyme preparation comprising a thermostable glycerol fermentation enzyme activity obtainable from the organism of claim 1, wherein the thermostable glycerol fermentation enzyme activity is capable of converting glycerol to 3-hydroxypropionaldehyde."],"number":8,"annotation":false,"title":false,"claim":true},{"lines":["An isolated enzyme preparation comprising a thermostable glycerol fermentation enzyme activity obtainable from the organism of claim 1, wherein the thermostable glycerol fermentation enzyme activity is capable of converting 3-hydroxypropionaldehyde to 1,3-propanediol oxidoreductase."],"number":9,"annotation":false,"title":false,"claim":true},{"lines":["A method of producing 1,3-propanediol from glycerol, the method comprising:
incubating glycerol with the enzyme preparation of claim 8, thereby converting the glycerol to 3-hydroxypropionaldehyde; and
adding a reducing agent capable of reducing 3-hydroxypropionaldehyde to 1,3-propanediol."],"number":10,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 11, wherein the reduction of 3-hydroxypropionaldehyde to 1,3-propanediol is catalysed by the enzyme preparation of claim 9."],"number":11,"annotation":false,"title":false,"claim":true},{"lines":["The method of any one of claims 2 to 7, or claims 10 and 11, further comprising the step of collecting 1,3-propanediol produced by the thermophilic organism."],"number":12,"annotation":false,"title":false,"claim":true},{"lines":["A method comprising the steps of claims 2 and 12, or the steps of claims 10 and 12, further comprising the step of polymerizing the 1,3-propanediol into a polymer."],"number":13,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 13, wherein the polymer is poly(1,3-propylene terephthalate) (PPT)."],"number":14,"annotation":false,"title":false,"claim":true},{"lines":["A method of cloning a polynucleotide sequence that encodes a thermostable glycerol fermentation enzyme, the method comprising; isolating polynucleotide coding sequences from the organism of claim 1; hybridizing a polynucleotide probe encoding at least a portion of a known glycerol fermentation enzyme to said polynucleotide coding sequences and isolating a polynucleotide sequence that binds to the polynucleotide probe."],"number":15,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 15, wherein a polymerase chain reaction using a second polynucleotide probe is used to amplify the polynucleotide sequence that binds to the polynucleotide probes."],"number":16,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 15 or claim 16, wherein the polynucleotide probe has the sequence of at least a portion of a known dhaB gene."],"number":17,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 17, wherein the dhaB gene is from Klebsiella."],"number":18,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 18, wherein at least one polynucleotide probe is selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 5 and SEQ ID NO: 6."],"number":19,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 19, wherein the polynucleotide probe and the second polynucleotide probe are SEQ ID NO: 1 and SEQ ID NO: 2."],"number":20,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 19, wherein the polynucleotide probe and the second polynucleotide probe are SEQ ID NO: 5 and SEQ ID NO: 6."],"number":21,"annotation":false,"title":false,"claim":true},{"lines":["A method of cloning a polynucleotide sequence that encodes a thermostable glycerol fermentation enzyme, the method comprising:
isolating DNA from a thermophilic organism of claim 1;
transforming a target organism that cannot grow anaerobically on glycerol with said DNA; and
identifying those transformed target organisms that contain the polynucleotide sequence that encodes an enzyme that ferments glycerol to 1,3-propanediol by their anaerobic growth on glycerol."],"number":22,"annotation":false,"title":false,"claim":true},{"lines":["A method of isolating a thermophilic organism that catalyzes the fermentation of glycerol to 1,3-propanediol, the method comprising:
incubating a sample containing thermophilic organisms in media containing glycerol as the primary carbon source; and
isolating at least one thermophilic organism that ferments glycerol into 1,3-propanediol by detecting production of 1,3-propanediol by the thermophilic organism."],"number":23,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 23, wherein the sample is incubated at a temperature in the range of 40°C to 100°C."],"number":24,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 23, wherein the sample is incubated under anaerobic conditions."],"number":25,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 23, wherein the sample is obtained from a natural source having a temperature of between 50°C to 100°C."],"number":26,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 23, further comprising the step of determining the production of acetate by the thermophilic organism."],"number":27,"annotation":false,"title":false,"claim":true},{"lines":["The method of any one of claims 23 to 27, wherein the organism is in the genus Caloramator or Thermobrechium."],"number":28,"annotation":false,"title":false,"claim":true},{"lines":["The method of any one of claims 23 to 28, wherein the organism is that of claim 1."],"number":29,"annotation":false,"title":false,"claim":true}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}