Proximity Assays Using Chemical Ligation And Hapten Transfer

  • Published: Jul 25, 2019
  • Earliest Priority: Nov 25 2014
  • Family: 11
  • Cited Works: 2
  • Cited by: 0
  • Cites: 0
  • Additional Info: Cited Works Full text
Abstract

Methods for in situ detecting proximity of two targets of interest featuring an antibody conjugated with a cleavable bridge component having a detectable moiety and an antibody conjugated with a non-cleavable bridge component. The bridge components each have a chemical ligation group adapted to form a covalent bond under particular conditions and when the targets are in close proximity. Following covalent bond formation, the cleavable bridge component can be cleaved from the antibody, effectively transferring the detectable moiety to the non-cleavable bridge component. Detection of the detectable moiety is indicative of the targets being in close proximity. The methods are compatible with both chromogenic and fluorogenic detection systems. The methods may be used to perform assays wherein one or more than one proximity event is detected on the same slide.


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Document History
  • Publication: Jul 25, 2019
  • Application: Nov 24, 2015
    AU AU 2015/352625 A
  • Priority: Nov 24, 2015
    EP EP 2015077484 W
  • Priority: Feb 17, 2015
    US US 201562116962 P
  • Priority: Nov 25, 2014
    US US 201462084452 P

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