Method Of Co-culturing Mammalian Muscle Cells And Motoneurons

Abstract

The invention provides a method of co-culturing mammalian muscle cells and mammalian motoneurons. The method comprises preparing one or more carriers coated with a covalently bonded monolayer of trimethoxysilylpropyl diethylenetriamine (DETA); suspending isolated fetal mammalian skeletal muscle cells in serum-free medium according to medium composition 1; suspending isolated fetal mammalian spinal motoneurons in serum-free medium according to medium composition 1; plating the suspended muscle cells onto the one or more carriers at a predetermined density and allowing the muscle cells to attach; plating the suspended motoneurons at a predetermined density onto the one or more carriers and allowing the motoneurons to attach; covering the one or more carriers with a mixture of medium composition 1 and medium composition 2; and incubating the carriers covered in the media mixture.


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Document History
  • Publication: May 16, 2017
  • Application: Jun 9, 2014
    US US 201414299802 A
  • Priority: Jun 9, 2014
    US US 201414299802 A
  • Priority: Apr 23, 2010
    US US 76599610 A
  • Priority: Apr 23, 2009
    US US 17195809 P

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