Methods For Phenotyping Of Intact Whole Tissues

  • Published: May 31, 2017
  • Earliest Priority: Sep 20 2013
  • Family: 11
  • Cited Works: 4
  • Cited by: 0
  • Cites: 1
  • Additional Info: Cited Works Published
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Abstract

In various embodiments, the present application teaches methods and compositions for tissue clearing in which whole organs and bodies are rendered macromolecule-permeable and optically-transparent, thereby exposing their cellular structure with intact connectivity. In some embodiments, the present application teaches PACT, a protocol for passive tissue clearing and immunostaining of intact organs. In other embodiments, the present application teaches RIMS, a refractive index matching media for imaging thick tissue. In yet other embodiments, the application teaches PARS, a method for whole-body clearing and immunolabeling.


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Document History
  • Publication: May 31, 2017
  • Application: Jul 30, 2014
    EP EP 14845995 A
  • Priority: Jul 30, 2014
    US US 2014/0048985 W
  • Priority: May 12, 2014
    US US 201461992103 P
  • Priority: Sep 20, 2013
    US US 201361880401 P

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