Method Of Preparing Plant-derived Vlps


Methods of preparing plant-derived virus like particles (VLPs) are provided. The method may comprise obtaining a plant, or plant matter comprising apoplast-localized VLPs, producing a protoplast/spheroplast fraction and apoplast fraction from the plant or plant matter, and recovering the apoplast fraction. The apoplast fraction comprises plant-derived VLPs. Alternatively, VLPs may be obtained from plant or plant matter comprising plant-derived VLPs by digesting the plant matter using a cell wall degrading enzyme composition to produced a digested fraction. The digested fraction is filtered to produced a filtered fraction, and the plant- derived VLPs are recovered from the filtered fraction.

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Document History
  • Publication: Mar 31, 2011
  • Application: Sep 21, 2010
    WO CA 2010001488 W
  • Priority: Sep 22, 2009
    US US 24478609 P

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