Method For Producing Temperature Sensitive Influenza A Viruses

Abstract

Vectors and methods for the production of influenza viruses suitable as recombinant influenza vaccines in cell culture are provided. Bi-directional expression vectors for use in a multi-plasmid influenza virus expression system are provided. Additionally, the invention provides methods of producing influenza viruses with enhanced ability to replicate in embryonated chicken eggs and/or cells (e.g., Vero and/or MDCK) and further provides influenza viruses with enhanced replication characteristics. A method of producing a cold adapted (ca) influenza virus that replicates efficiently at, e.g., 25° C. (and immunogenic compositions comprising the same) is also provided.


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Document History
  • Publication: Feb 14, 2012
  • Application: Oct 20, 2008
    US US 25413108 A
  • Priority: Oct 20, 2008
    US US 25413108 A
  • Priority: May 20, 2005
    US US 13334505 A
  • Priority: Mar 2, 2005
    US US 65737205 P
  • Priority: Jan 13, 2005
    US US 64327805 P
  • Priority: Dec 22, 2004
    US US 1862404 A
  • Priority: Dec 1, 2004
    US US 63189204 P
  • Priority: Jun 12, 2004
    US US 57896204 P
  • Priority: May 24, 2004
    US US 57411704 P
  • Priority: Dec 23, 2003
    US US 53216403 P
  • Priority: Apr 25, 2003
    US US 42382803 A
  • Priority: Apr 10, 2003
    US US 46236103 P
  • Priority: Mar 24, 2003
    US US 45769903 P
  • Priority: Oct 23, 2002
    US US 42070802 P
  • Priority: Oct 18, 2002
    US US 41980202 P
  • Priority: Sep 12, 2002
    US US 41057602 P
  • Priority: Jul 9, 2002
    US US 39498302 P
  • Priority: Apr 26, 2002
    US US 37567502 P

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