{"search_session":{},"preferences":{"l":"ru","queryLanguage":"ru"},"patentId":"175-798-163-981-811","frontPageModel":{"patentViewModel":{"ref":{"entityRefType":"PATENT","entityRefId":"175-798-163-981-811"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":8892,"type":"PATENT","title":"University of Wisconsin Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":15082,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8214,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners = \"Wisconsin University\" , \"University of Wisconsin\", \"Wisconsin Univ\", \" Univ Wisconsin\" , \"Wisco* Alum* Res* Found*\".
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Search Applicants and Owners = \"Wisconsin University\" , \"University of Wisconsin\", \"Wisconsin Univ\", \" Univ Wisconsin\" , \"Wisco* Alum* Res* Found*\".
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(a) providing a cell culture substrate by: (i) preparing an interface between an aqueous phase and a liquid crystalline phase; and (ii) depositing a protein or peptide layer at said interface, wherein the protein or peptide layer functions as an extracellular matrix component;\n
(b) seeding living cells onto the protein or peptide layer of said cell culture substrate;\n
(c) maintaining said living cells under culture conditions; and\n
(d) detecting reorganization of the liquid crystalline phase, said reorganization indicating cell-induced changes between the living cells and the cell culture substrate."],"number":1,"annotation":false,"title":false,"claim":true},{"lines":["The method according to claim 1 wherein said protein or peptide layer comprises at least one protein or peptide that is an extracellular matrix component."],"number":2,"annotation":false,"title":false,"claim":true},{"lines":["The method according to claim 1 wherein said protein or peptide layer comprises gelatin, laminin collagen, elastin, or mixtures thereof."],"number":3,"annotation":false,"title":false,"claim":true},{"lines":["The method according to claim 1 wherein said protein or peptide layer comprises a synthetic polypeptide."],"number":4,"annotation":false,"title":false,"claim":true},{"lines":["The method according to claim 1 wherein the liquid crystalline phase is a liquid crystal elastomer."],"number":5,"annotation":false,"title":false,"claim":true},{"lines":["The method according to claim 1 further comprising the step of forming a polyelectrolyte multilayer (PEM) film at the interface between said aqueous phase and liquid crystalline phase and wherein the step of depositing a protein or peptide layer at said interface further comprises incorporating said protein or peptide layer into the PEM film."],"number":6,"annotation":false,"title":false,"claim":true},{"lines":["The method according to claim 1 wherein said living cells are human embryonic stem cells."],"number":7,"annotation":false,"title":false,"claim":true}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}