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Search Applicants and Owners separately: louisiana state. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 2862
Search Applicants and Owners separately: louisiana state. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 2862
a modified transposase gene operably linked to a first promoter, wherein the nucleotide sequence 3′ to the first promoter comprises a modified Kozak sequence, and wherein a plurality of the first twenty codons of the transposase gene are modified from the wild-type sequence by changing the nucleotide at the third base position of the codon to an adenine or thymine without modifying the amino acid encoded by the codon;\n
a multiple cloning site capable of receiving a gene of interest;\n
transposon insertion sequences recognized by a transposase encoded by the modified transposase gene, wherein the transposon insertion sequences flank the multiple cloning site; and,\n
one or more insulator elements located between the transposon insertion sequences and the multiple cloning site, wherein the one or more insulator elements comprise a lysozyme replicator element, a combination of a lysozyme replicator element and an HS4 element, or a matrix attachment region element, wherein when the gene of interest is inserted into the multiple cloning site, the start codon of the gene of interest is located no more than 2502 bp and no less than 1652 bp from the one or more insulator elements located 5′ to the start codon of the gene of interest."],"number":1,"annotation":false,"title":false,"claim":true},{"lines":["The vector of claim 1, further comprising a second promoter, wherein the second promoter is SEQ ID NO: 14 or SEQ ID NO: 15."],"number":2,"annotation":false,"title":false,"claim":true},{"lines":["The vector of claim 1, further comprising a gene encoding for an antibody inserted into the multiple cloning site."],"number":3,"annotation":false,"title":false,"claim":true},{"lines":["The vector of claim 3, wherein the vector comprises SEQ ID NO: 25."],"number":4,"annotation":false,"title":false,"claim":true},{"lines":["A transposon-based vector comprising:\n
a modified transposase gene operably linked to a first promoter, wherein the nucleotide sequence 3′ to the first promoter comprises a modified Kozak sequence, and wherein a plurality of the first twenty codons of the transposase gene are modified from the wild-type sequence by changing the nucleotide at the third base position of the codon to an adenine or thymine without modifying the amino acid encoded by the codon;\n
one or more genes of interest encoding an antibody operably-linked to one or more additional promoters, wherein the one or more genes of interest encoding the antibody and their operably-linked promoters are flanked by transposon insertion sequences recognized by a transposase encoded by the modified transposase gene; and,\n
one or more insulator elements located between the transposon insertion sequences and the one or more genes of interest encoding the antibody, wherein the start codon of the gene of interest is located about 2502 bp to about 1652 bp from the one or more insulator elements located 5′ to the start codon of the gene of interest, and wherein the one or more insulator elements comprise a lysozyme replicator element, a combination of a lysozyme replicator element and an HS4 element, or a matrix attachment region element."],"number":5,"annotation":false,"title":false,"claim":true},{"lines":["A method of producing an antibody comprising:\n
transfecting a cell with a vector comprising\n
a modified transposase gene operably linked to a first promoter, wherein the nucleotide sequence 3′ to the first promoter comprises a modified Kozak sequence, and wherein a plurality of the first twenty codons of the transposase gene are modified from the wild-type sequence by changing the nucleotide at the third base position of the codon to an adenine or thymine without modifying the amino acid encoded by the codon;\n
one or more genes of interest encoding an antibody operably-linked to one or more additional promoters, wherein the one or more genes of interest encoding the antibody and their operably-linked promoters are flanked by transposon insertion sequences recognized by a transposase encoded by the modified transposase gene; and,\n
one or more insulator elements located between the transposon insertion sequences and the one or more genes of interest encoding the antibody, wherein the start codon of the gene of interest is located about 2502 bp to about 1652 bp from the one or more insulator elements located 5′ to the start codon of the gene of interest, and wherein the one or more insulator elements comprise a lysozyme replicator element, a combination of a lysozyme replicator element and an HS4 element, or a matrix attachment region element;\n
culturing the transfected cell in culture medium;\n
permitting the cell to release the antibody into the culture medium;\n
collecting the culture medium; and,\n
isolating the antibody."],"number":6,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 6 wherein the vector comprises SEQ ID NO:25."],"number":7,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 6 wherein the antibody is a human antibody."],"number":8,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 6 wherein the antibody is a monoclonal antibody."],"number":9,"annotation":false,"title":false,"claim":true}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}