Absolute Quantitation Of Protein Contents Based On Exponentially Modified Protein Abundance Index By Mass Spectrometry

Abstract

The present inventor has established protein abundance index (PAI, π) to determine the protein contents in a protein mixture solution using nanoLC-MSMS data. Digested peptides were analyzed by nanoLC-MS/MS and the obtained results were applied to a Mascot protein identification algorism based on tandem mass spectra. For absolute quantitation, PAI was converted to exponentially modified PAI (EMPAI, m π), which is proportional to protein contents in the protein mixture. EMPAI was successfully applied to comprehensive protein expression analysis and performed a comparison study between gene and protein expression in an HCT116 human cancer cells. Accordingly, the present invention provides a method and a computer program for quantifying the protein contents based on the protein abundance index.


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Document History
  • Publication: Mar 22, 2011
  • Application: Jul 4, 2005
    US US 65859205 A
  • Priority: Jul 4, 2005
    US US 65859205 A
  • Priority: Jul 4, 2005
    JP 2005012705 W
  • Priority: Jul 29, 2004
    US US 59196304 P

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