{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"172-021-144-149-42X","frontPageModel":{"patentViewModel":{"ref":{"entityRefType":"PATENT","entityRefId":"172-021-144-149-42X"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":10802,"type":"PATENT","title":"Brown University - Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":4664,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8267,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately: \"Brown Univ*\"
Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 2360
Search Applicants and Owners separately: \"Brown Univ*\"
Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 2360
a. adding a genetically modified fungus to a fermentation mixture comprising a cellulosic biomass material or fermentation substrates derived from said cellulosic biomass material, wherein said genetic modification to said fungus comprises introduction of an expression vector comprising the coding sequence of a yeast protein of the Sm-like superfamily, and wherein said genetic modification results in elevated tolerance to at least one inhibitory chemical compound formed during biomass pretreatment as compared to without the genetic modification;\n
b. allowing said microorganism to ferment and produce alcohol; and\n
c. recovering alcohol produced by fermentation with said fungus."],"number":1,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein said fungus is a fungal species selected from Saccharomyces sp., Kluyveromyces sp., Pichia sp., Candida sp., and Schizosaccharomycetes sp."],"number":2,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 2, wherein said fungal species is yeast selected from S. cerevisiae or P. pastoris. "],"number":3,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 3, wherein said yeast protein is a yeast Sm or Lsm protein."],"number":4,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 4, wherein said protein comprises an amino acid sequence selected from any one of SEQ ID NOS: 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48 or 50, or a protein sequence that shares at least 95% sequence identity therewith."],"number":5,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein said expression vector is a replicative vector or an integrative vector."],"number":6,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein said at least one inhibitory chemical compound formed during biomass pretreatment is selected from the group consisting of an acetate salt, vanillin, furfural, hydroxymethylfurfural (HMF) and H2O2."],"number":7,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 7, wherein said acetate salt is selected from the group consisting of sodium acetate, ammonium acetate and potassium acetate."],"number":8,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein said enhanced tolerance is characterized by ability to grow in a media containing sodium acetate at a concentration of 195 mM."],"number":9,"annotation":false,"title":false,"claim":true}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}