Control Nucleic Acid Sequences For Use In Sequencing-by-synthesis And Methods For Designing The Same

Abstract

A method for nucleic acid sequencing includes (a) disposing a plurality of template polynucleotide strands in a plurality of defined spaces disposed on a sensor array, at least some of the template polynucleotide strands comprising a test or control sequence; (b) exposing a plurality of the template polynucleotide strands in the defined spaces to a series of flows of nucleotide species flowed according to a predetermined ordering; and (c) determining sequence information for a plurality of the template polynucleotide strands in the defined spaces based on the flows of nucleotide species to generate a plurality of sequencing reads corresponding to the template polynucleotide strands, wherein the test or control sequence comprises a sequence determined by identifying, using a variant caller, loci with systematic errors present in a plurality of sequencing runs included in a training set of sequencing runs.


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Document History
  • Publication: Mar 27, 2018
  • Application: Jul 23, 2014
    US US 201414338682 A
  • Priority: Jul 23, 2014
    US US 201414338682 A
  • Priority: Jul 26, 2013
    US US 201361858828 P

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