{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"167-226-963-123-248","frontPageModel":{"patentViewModel":{"ref":{"entityRefType":"PATENT","entityRefId":"167-226-963-123-248"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":10795,"type":"PATENT","title":"The Ohio State University - Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":7061,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8260,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately: \"ohio state univ*\"
Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 5396
Search Applicants and Owners separately: \"ohio state univ*\"
Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 5396
(a) substitution at amino acid position 454 with proline, and insertion of the amino acids alanine and threonine after amino acid position 454;\n
(b) substitution at amino acid position 454 with glycine, and insertion of the amino acids alanine and valine after amino acid position 454;\n
(c) substitution at amino acid position 454 with alanine, and insertion of the amino acids alanine and threonine after amino acid position 454;\n
(d) substitution at amino acid position 454 with proline, and insertion of the amino acids alanine and alanine after amino acid position 454;\n
(e) substitution at amino acid position 454 with alanine, and insertion of the amino acids alanine and alanine after amino acid position 454; and\n
(f) substitution at amino acid position 454 with glycine, and insertion of the amino acids alanine and threonine after amino acid position 454."],"number":7,"annotation":false,"title":false,"claim":true},{"lines":["The isolated insecticidal Bacillus thuringiensis Cry4Ba protein according to claim 6, wherein the aspartic acid at position 454 is substituted with proline and the second additional amino acid inserted immediately after position 454 is threonine or valine."],"number":8,"annotation":false,"title":false,"claim":true},{"lines":["The isolated insecticidal Bacillus thuringiensis Cry4Ba protein according to claim 6, wherein, the aspartic acid at position 454 is substituted with glycine and the second additional amino acid inserted immediately after position 454 is threonine or valine."],"number":9,"annotation":false,"title":false,"claim":true},{"lines":["An isolated insecticidal Bacillus thuringiensis Cry4Ba protein that has the sequence SEQ ID NO: 1 that is modified by one or more amino acid substitution at position 578, 580, or 581 with an amino acid selected from the group consisting of alanine, glycine, phenylalanine, tyrosine, tryptophan, threonine and serine, and wherein the modified Cry4Ba protein has enhanced toxicity against Anopheles or Aedes or both as compared to Cry4Ba protein that has the sequence SEQ ID NO: 1."],"number":10,"annotation":false,"title":false,"claim":true},{"lines":["The isolated insecticidal Bacillus thuringiensis Cry4Ba protein according to claim 10, wherein the one or more amino acid substitutions are: the amino acid at position 578 is replaced with alanine; the amino acid at position 580 is replaced with phenylalanine or tyrosine; or the amino acid at position 581 is replaced with alanine or phenylalanine."],"number":11,"annotation":false,"title":false,"claim":true},{"lines":["An isolated insecticidal Bacillus thuringiensis Cry4Ba protein that has the sequence SEQ ID NO: 1 that is modified by one or more amino acid substitutions at position 578, 580, or 581, wherein the one or more amino acid substitutions are: the amino acid at position 578 is replaced with alanine; the amino acid at position 580 is replaced with phenylalanine or tyrosine; or the amino acid at position 581 is replaced with alanine or phenylalanine, and further modified by substitution of aspartic acid at position 454 with proline, and the insertion of amino acids alanine and threonine immediately after position 454, and wherein the modified Cry4Ba protein has enhanced toxicity against Anopheles or Aedes or both as compared to Cry4Ba protein that has the sequence SEQ ID NO: 1."],"number":12,"annotation":false,"title":false,"claim":true},{"lines":["An isolated nucleic acid molecule encoding a modified Cry4Ba protein that has the sequence SEQ ID NO: 1 that is modified by substitution of the amino acid aspartic acid at position 454 with proline, glycine, alanine, threonine or serine; and insertion of two additional amino acids immediately after position 454, wherein the additional amino acids are chosen from the group consisting of: glycine, alanine, valine, leucine, isoleucine, methionine, proline, threonine, and serine, and wherein the modified Cry4Ba protein exhibits enhanced toxicity to Culex as compared to Cry4Ba protein that has the sequence SEQ ID NO: 1."],"number":13,"annotation":false,"title":false,"claim":true},{"lines":["A vector comprising an isolated nucleic acid molecule encoding a modified Cry4Ba protein, wherein the modified Cry4Ba protein has the sequence SEQ ID NO: 1 that is modified by substitution of the amino acid aspartic acid at position 454 with proline, glycine, alanine, threonine or serine; and insertion of two additional amino acids immediately after position 454, wherein the additional amino acids are chosen from the group consisting of: glycine, alanine, valine, leucine, isoleucine, methionine, proline, threonine, and serine, and wherein the modified Cry4Ba protein exhibits enhanced toxicity to Culex as compared to Cry4Ba protein that has the sequence SEQ ID NO: 1."],"number":14,"annotation":false,"title":false,"claim":true},{"lines":["A host cell comprising an isolated nucleic acid molecule encoding a modified Cry4Ba protein, wherein the modified Cry4Ba protein has the sequence SEQ ID NO: 1 that is modified by substitution of the amino acid aspartic acid at position 454 with proline, glycine, alanine, threonine or serine; and insertion of two additional amino acids immediately after position 454, wherein the additional amino acids are chosen from the group consisting of: glycine, alanine, valine, leucine, isoleucine, methionine, proline, threonine, and serine, and wherein the modified Cry4Ba protein exhibits enhanced toxicity to Culex as compared to Cry4Ba protein that has the sequence SEQ ID NO 1."],"number":15,"annotation":false,"title":false,"claim":true},{"lines":["A method for reducing populations of target mosquitoes that are vectors of disease, by delivering into a habitat of the target mosquitoes one or more isolated Cry4Ba proteins according to claim 7 as an insecticidal agent."],"number":16,"annotation":false,"title":false,"claim":true},{"lines":["Insecticidal compositions comprising mutant toxins including one or more modified Cry4Ba proteins, and an agriculturally acceptable carrier, diluent and/or excipient, wherein the modified Cry4Ba protein has the sequence SEQ ID NO: 1 that is modified by substitution of the amino acid aspartic acid at position 454 with proline, glycine, alanine, threonine or serine; and insertion of two additional amino acids immediately after position 454, wherein the additional amino acids are chosen from the group consisting of: glycine, alanine, valine, leucine, isoleucine, methionine, proline, threonine, and serine, and wherein the modified Cry4Ba protein exhibits enhanced toxicity to Culex as compared to Cry4Ba protein that has the sequence SEQ ID NO: 1."],"number":17,"annotation":false,"title":false,"claim":true},{"lines":["The nucleic acid of claim 13, wherein the modified insecticidal Bacillus thuringiensis Cry4Ba protein has a modification selected from the group consisting of:\n
(a) substitution at amino acid position 454 with proline, and insertion of the amino acids alanine and threonine immediately after amino acid position 454;\n
(b) substitution at amino acid position 454 with glycine, and insertion of the amino acids alanine and valine immediately after amino acid position 454;\n
(c) substitution at amino acid position 454 with alanine, and insertion of the amino acids alanine and threonine immediately after amino acid position 454;\n
(d) substitution at amino acid position 454 with proline, and insertion of the amino acids alanine and alanine immediately after amino acid position 454;\n
(e) substitution at amino acid position 454 with alanine, and insertion of the amino acids alanine and alanine immediately after amino acid position 454; and\n
(f) substitution at amino acid position 454 with glycine, and insertion of the amino acids alanine and threonine immediately after amino acid position 454."],"number":18,"annotation":false,"title":false,"claim":true},{"lines":["The vector of claim 14, wherein the modified Cry4Ba protein has a modification selected from the group consisting of:\n
(a) substitution at amino acid position 454 with proline, and insertion of the amino acids alanine and threonine immediately after amino acid position 454;\n
(b) substitution at amino acid position 454 with glycine, and insertion of the amino acids alanine and valine immediately after amino acid position 454;\n
(c) substitution at amino acid position 454 with alanine, and insertion of the amino acids alanine and threonine immediately after amino acid position 454;\n
(d) substitution at amino acid position 454 with proline, and insertion of the amino acids alanine and alanine immediately after amino acid position 454;\n
(e) substitution at amino acid position 454 with alanine, and insertion of the amino acids alanine and alanine immediately after amino acid position 454; and\n
(f) substitution at amino acid position 454 with glycine, and insertion of the amino acids alanine and threonine immediately after amino acid position 454."],"number":19,"annotation":false,"title":false,"claim":true},{"lines":["The host cell of claim 15, wherein the modified Cry4Ba protein has a modification selected from the group consisting of:\n
(a) substitution at amino acid position 454 with proline, and insertion of the amino acids alanine and threonine immediately after amino acid position 454;\n
(b) substitution at amino acid position 454 with glycine, and insertion of the amino acids alanine and valine immediately after amino acid position 454;\n
(c) substitution at amino acid position 454 with alanine, and insertion of the amino acids alanine and threonine immediately after amino acid position 454;\n
(d) substitution at amino acid position 454 with proline, and insertion of the amino acids alanine and alanine immediately after amino acid position 454;\n
(e) substitution at amino acid position 454 with alanine, and insertion of the amino acids alanine and alanine immediately after amino acid position 454; and\n
(f) substitution at amino acid position 454 with glycine, and insertion of the amino acids alanine and threonine immediately after amino acid position 454."],"number":20,"annotation":false,"title":false,"claim":true},{"lines":["The insecticidal composition of claim 17, wherein the modified Cry4Ba protein has a modification selected from the group consisting of: (a) substitution at amino acid position 454 with proline, and insertion of the amino acids alanine and threonine immediately after amino acid position 454; (b) substitution at amino acid position 454 with glycine, and insertion of the amino acids alanine and valine immediately after amino acid position 454; (c) substitution at amino acid position 454 with alanine, and insertion of the amino acids alanine and threonine immediately after amino acid position 454; (d) substitution at amino acid position 454 with proline, and insertion of the amino acids alanine and alanine immediately after amino acid position 454; (e) substitution at amino acid position 454 with alanine, and insertion of the amino acids alanine and alanine immediately after amino acid position 454; and (f) substitution at amino acid position 454 with glycine, and insertion of the amino acids alanine and threonine immediately after amino acid position 454."],"number":21,"annotation":false,"title":false,"claim":true},{"lines":["An isolated nucleic acid molecule encoding a modified Bacillus thuringiensis Cry4Ba protein that has the sequence SEQ ID NO: 1 that is modified by one or more amino acid substitutions at position 578, 580, or 581 with an amino acid selected from the group consisting of: alanine, glycine, phenylalanine, tyrosine, tryptophan, threonine and serine, and wherein the modified Cry4Ba protein exhibits enhanced toxicity to Anopheles or Aedes or both as compared to Cry4Ba protein that has the sequence SEQ ID NO: 1."],"number":22,"annotation":false,"title":false,"claim":true},{"lines":["The nucleic acid molecule according to claim 22, wherein the modified Cry4Ba protein comprises one or more of the following amino acid substitutions: the amino acid at position 578 is replaced with alanine; the amino acid at position 580 is replaced with phenylalanine or tyrosine; or the amino acid at position 581 is replaced with alanine or phenylalanine."],"number":23,"annotation":false,"title":false,"claim":true},{"lines":["A vector comprising an isolated nucleic acid molecule encoding a modified Bacillus thuringiensis Cry4Ba protein that has the sequence SEQ ID NO: 1 that is modified by one or more amino acid substitutions at position 578, 580, or 581 with an amino acid selected from the group consisting of: alanine, glycine, phenylalanine, tyrosine, tryptophan, threonine and serine, and wherein the modified Cry4Ba protein exhibits enhanced toxicity to Anopheles or Aedes or both as compared to Cry4Ba protein that has the sequence SEQ ID NO: 1."],"number":24,"annotation":false,"title":false,"claim":true},{"lines":["The vector according to claim 24, wherein the modified Cry4Ba protein comprises one or more of the following amino acid substitutions: the amino acid at position 578 is replaced with alanine; the amino acid at position 580 is replaced with phenylalanine or tyrosine; or the amino acid at position 581 is replaced with alanine or phenylalanine."],"number":25,"annotation":false,"title":false,"claim":true},{"lines":["A host cell comprising an isolated nucleic acid molecule encoding a modified Bacillus thuringiensis Cry4Ba protein that has the sequence SEQ ID NO: 1 that is modified by one or more amino acid substitutions at position 578, 580, or 581 with an amino acid selected from the group consisting of: alanine, glycine, phenylalanine, tyrosine, tryptophan, threonine and seine, and wherein the modified Cry4Ba protein exhibits enhanced toxicity to Anopheles or Aedes or both as compared to Cry4Ba protein that has the sequence SEQ ID NO: 1."],"number":26,"annotation":false,"title":false,"claim":true},{"lines":["The host cell according to claim 26, wherein the modified Cry4Ba protein comprises one or more of the following amino acid substitutions: the amino acid at position 578 is replaced with alanine; the amino acid at position 580 is replaced with phenylalanine or tyrosine; or the amino acid at position 581 is replaced with alanine or phenylalanine."],"number":27,"annotation":false,"title":false,"claim":true},{"lines":["An insecticidal composition comprising one or more Bacillus thuringiensis modified Cry4Ba proteins and an agriculturally acceptable carrier, diluent and/or excipient, wherein the modified Cry4Ba protein has the sequence SEQ ID NO: 1 that is modified by one or more amino acid substitutions at position 578, 580, or 581 with an amino acid selected from the group consisting of: alanine, glycine, phenylalanine, tyrosine, tryptophan, threonine and serine, and wherein the modified Cry4Ba protein exhibits enhanced toxicity to Anopheles or Aedes or both as compared to Cry4Ba protein that has the sequence SEQ ID NO: 1."],"number":28,"annotation":false,"title":false,"claim":true},{"lines":["The insecticidal composition of claim 28, wherein the modified Cry4Ba protein comprises one or more of the following amino acid substitutions: the amino acid at position 578 is replaced with alanine; the amino acid at position 580 is replaced with phenylalanine or tyrosine; or the amino acid at position 581 is replaced with alanine or phenylalanine."],"number":29,"annotation":false,"title":false,"claim":true}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}