Agrobacterium Mediated Transformation Of Moulds, In Particular Those Belonging To The Genus Aspergillus

  • Published: Oct 14, 1998
  • Earliest Priority: Apr 07 1997
  • Family: 2
  • Cited Works: 6
  • Cited by: 3
  • Cites: 2
  • Additional Info: Cited Works Published
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Abstract

The invention relates to Agrobacterium mediated transformation of moulds comprising species of the fungal sub-divisions Ascomycotina, Basidiomycotina, Deuteromycotina, Mastigomycotina, and Zygomycotina. Examples demonstrate the transformation of Aspergillus awamori (both protoplasts and conidia), Aspergillus nidulans, Aspergillus niger, Colletotrichum gloeosporioides, Fusarium solani pisi, Neurospora crassa, Trichoderma reesei, and Pleurotus ostreatus (all conidia), and Fusarium graminearum (both conidia and rehydrated freeze dried ATCC material). Especially for Aspergillus awamori the transformation frequency is much higher than with conventional mould transformation techniques. Information on a deposit of a micro-organism under the Budapest Treaty is given above on page 16, lines 26-29. In agreement with Rule 28 (4) EPC, or a similar arrangement for a State not being a Contracting State of the EPC, it is hereby requested that a sample of such deposit, when requested, will be submitted to an expert only.


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Document History
  • Publication: Oct 14, 1998
  • Application: Apr 7, 1997
    EP EP 97201022 A
  • Priority: Apr 7, 1997
    EP EP 97201022 A

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