Systems And Methods For Detecting Nucleic Acids

Abstract

Methods and kits for detecting a target nucleic acid in a sample are described. In some embodiments, the sample to be analyzed includes a primer which hybridizes to at least a portion of the target nucleic acid, a probe having a first region which hybridizes to at least a portion of the target nucleic acid and a second region having a detectable label, a polymerase which extends the hybridized primer and an enzyme comprising nuclease activity that can cleave the hybridized hybridization probe to thereby release a labeled probe fragment. In some embodiments, the sample can then be contacted with a solid support comprising surface bound capture probes which can hybridize to the labeled probe fragment(s). These capture probes more readily bind to the probe fragment(s) than to the intact hybridization probe. The label can then be detected on the support surface. In this manner, improved discrimination between the probe fragments and the intact hybridization probes can be achieved.


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Document History
  • Publication: Jul 10, 2008
  • Application: Dec 28, 2007
    WO US 2007/0089010 W
  • Priority: Jan 18, 2007
    US US 88096407 P
  • Priority: Jan 16, 2007
    US US 88042807 P
  • Priority: Dec 29, 2006
    US US 87761006 P

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