{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"154-544-140-918-324","frontPageModel":{"patentViewModel":{"ref":{"entityRefType":"PATENT","entityRefId":"154-544-140-918-324"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":6878,"type":"PATENT","title":"Flinders Univ Patent Portfolio","description":"
Search applicants = 'Flinders University' 'University Flinders' ' Flinders Univ' 'Univ Flinders'
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Search Owners (US) = 'Flinders University' 'University Flinders' ' Flinders Univ' 'Univ Flinders'
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Add to collectionSearch Applicants and Owners separately:flinder univ*. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 187
Search applicants = 'Flinders University' 'University Flinders' ' Flinders Univ' 'Univ Flinders'
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Search Owners (US) = 'Flinders University' 'University Flinders' ' Flinders Univ' 'Univ Flinders'
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Add to collectionSearch Applicants and Owners separately:flinder univ*. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 187
(i) performing a multiplicity of amplification reactions of nucleic acid molecules derived from said clonal cell population, each said amplification reaction utilising an upstream primer which is common to all reactions and is directed to a conserved region of the upstream gene segment and a downstream primer which is selected from a group of primers each of which is specific for a different individual downstream gene segment;\n
(ii) identifying which of said downstream primers enables amplification of said downstream gene segment;\n
(iii) repeating steps (i) and (ii) wherein said downstream primer is common to all reactions and is directed to a conserved region of the downstream gene segment and said upstream primer is selected from a group of primers each of which is specific for a different individual upstream gene segment."],"number":11,"annotation":false,"title":false,"claim":true},{"lines":["The method according to claim 11 wherein said amplification reaction is a polymerase chain reaction."],"number":12,"annotation":false,"title":false,"claim":true},{"lines":["The method according to claim 10 wherein said identification method comprises:\n
(i) performing a multiplicity of amplification reactions of nucleic acid molecules derived from said clonal cell population, each said amplification reaction utilising an upstream primer which is selected from a group of primers each of which is specific for a different individual upstream gene segment and a downstream primer which is selected from a group of primers each of which is specific for a different individual downstream gene segment; and\n
(ii) identifying which of said upstream and downstream primers enables amplification of said gene segments."],"number":13,"annotation":false,"title":false,"claim":true},{"lines":["The method according to claim 13 wherein said amplification reaction is a polymerase chain reaction."],"number":14,"annotation":false,"title":false,"claim":true},{"lines":["The method according to any one of claims 1, 2, 3-6 and 7-9 wherein said identification method comprises a nucleic acid hybridisation step, which comprises hybridisation reactions performed utilizing a microarray."],"number":15,"annotation":false,"title":false,"claim":true},{"lines":["The method according to any one of claims 1, 2, 3-6 and 7-9 wherein said identification method comprises a nucleic acid sequencing step."],"number":16,"annotation":false,"title":false,"claim":true},{"lines":["The method according to claim 16 wherein said nucleic acid sequencing is pyrosequencing."],"number":17,"annotation":false,"title":false,"claim":true},{"lines":["The method according to claim 16 wherein said nucleic acid sequencing is minisequencing."],"number":18,"annotation":false,"title":false,"claim":true},{"lines":["A method of detecting or monitoring a clonal population of cells in a mammal, which clonal cells are characterised by a rearranged immunoglobulin or T cell receptor variable region gene and which gene is analysed in accordance with the method of claim 1 to identify one or more of the V, D or J gene segments of said rearranged gene, said method comprising screening the nucleic acid molecules of a biological sample from said mammal for the presence of said V, D or J gene segment."],"number":19,"annotation":false,"title":false,"claim":true},{"lines":["A method of diagnosing and/or monitoring a mammalian disease condition characterised by a clonal population of cells, which clonal cells are characterised by a rearranged immunoglobulin or T cell receptor variable region gene and which gene is analysed in accordance with the method of claim 1 to identify one or more of the V, D or J gene segments of said rearranged gene, said method comprising screening the nucleic acid molecules of a biological sample from said mammal for the presence of said V, D or J gene segment."],"number":20,"annotation":false,"title":false,"claim":true},{"lines":["The method according to claim 19 or 20 wherein said clonal population of cells is an immune cell population."],"number":21,"annotation":false,"title":false,"claim":true},{"lines":["The method according to claim 21 wherein said clonal population of immune cells is a neoplastic population of cells."],"number":22,"annotation":false,"title":false,"claim":true},{"lines":["The method according to claim 21 wherein said immune cell population is a T cell population."],"number":23,"annotation":false,"title":false,"claim":true},{"lines":["The method according to claim 21 wherein said immune cell population is a B cell population."],"number":24,"annotation":false,"title":false,"claim":true},{"lines":["The method according to claim 21 wherein the gene segments which are identified are the V and D gene segments."],"number":25,"annotation":false,"title":false,"claim":true},{"lines":["The method according to claim 21 wherein the gene segments which are identified are the V and J gene segments."],"number":26,"annotation":false,"title":false,"claim":true},{"lines":["The method according to claim 21 wherein the gene segments which are identified are the D and J gene segments."],"number":27,"annotation":false,"title":false,"claim":true},{"lines":["The method according to claim 20 wherein said condition is a neoplastic condition."],"number":28,"annotation":false,"title":false,"claim":true},{"lines":["The method according to claim 28 wherein said neoplastic condition is a malignant neoplastic condition."],"number":29,"annotation":false,"title":false,"claim":true},{"lines":["The method according to claim 28 wherein said neoplastic condition is a non-malignant neoplastic condition."],"number":30,"annotation":false,"title":false,"claim":true},{"lines":["The method according to claim 20 wherein said condition is immunodeficiency and said screening is directed to detecting specific immune cell expansion."],"number":31,"annotation":false,"title":false,"claim":true},{"lines":["The method according to claim 20 wherein said condition is an immune response and said screening is directed to detecting specific immune cell expansion."],"number":32,"annotation":false,"title":false,"claim":true},{"lines":["A method of enriching for a population of nucleic acid molecules in a biological sample, which nucleic acid molecules are characterised by a rearranged immunoglobulin or T cell receptor variable region gene and which gene is analysed in accordance with the method of claim 1 to identify one or more of the V, D or J gene segments of said rearranged gene, said method comprising increasing the ratio of nucleic acid molecules comprising said V, D or J gene segments relative to nucleic acid molecules which do not comprise said V, D or J gene segments."],"number":33,"annotation":false,"title":false,"claim":true},{"lines":["The method according to claim 33 wherein the gene segments which are identified are the V and D gene segments."],"number":34,"annotation":false,"title":false,"claim":true},{"lines":["The method according to claim 33 wherein the gene segments which are identified are the V and J gene segments."],"number":35,"annotation":false,"title":false,"claim":true},{"lines":["The method according to claim 33 wherein the gene segments which are identified are the D and J gene segments."],"number":36,"annotation":false,"title":false,"claim":true}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}