{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"151-576-517-115-562","frontPageModel":{"patentViewModel":{"ref":{"entityRefId":"151-576-517-115-562","entityRefType":"PATENT"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":8903,"type":"PATENT","title":"Johns Hopkins Univ Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":17938,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8218,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search applicants and owners= \"Johns Hopkins Univ\", \"Univ Johns Hopkins\"
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Total patents: 12941
Search applicants and owners= \"Johns Hopkins Univ\", \"Univ Johns Hopkins\"
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Total patents: 12941
enriching the selected protein in the biological sample to form an enriched sample;\n
fragmenting the selected protein in the enriched sample using a site-specific endoprotease to form a fragmented, enriched sample comprising a selected peptide;\n
spiking the fragmented, enriched sample with a known amount of a heavy-isotope labeled form of the selected peptide;\n
subjecting the spiked fragmented, enriched sample to liquid chromatography to form output fractions having distinct peptide profiles;\n
directing the output fractions to a triple quadrupole mass spectrometer to form product ions;\n
detecting selected product ions of the selected peptide representing wild type and/or mutant forms of the selected protein and product ions of the heavy-isotope labeled form of the selected peptide."],"number":1,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1 wherein the step of enriching employs immunoprecipitation of the selected protein."],"number":2,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 2 wherein immunoprecipitation is carried out using an antibody which is attached to a bead."],"number":3,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 3 wherein the selected protein is eluted from the antibody using 3% acetic acid."],"number":4,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 3 wherein the bead is magnetic."],"number":5,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1 wherein the endoprotease is trypsin."],"number":6,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1 wherein a ratio of wild type to mutant forms of the selected protein is calculated."],"number":7,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1 wherein the biological sample is a tissue sample."],"number":8,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1 wherein the biological sample is a biological fluid."],"number":9,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1 wherein the biological sample comprises neoplastic cells or proteins from neoplastic cells."],"number":10,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1 wherein the biological sample comprises pre-malignant cells or proteins from pre-malignant cells."],"number":11,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1 wherein the biological sample comprises at least 25 fmole of the selected protein in 1 mg of total protein."],"number":12,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1 wherein the biological sample comprises at least 300 cells."],"number":13,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1 wherein the biological sample comprises at least 500 cells."],"number":14,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1 wherein the biological sample comprises at least 6,000 cells."],"number":15,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1 wherein the liquid chromatography is high performance liquid chromatography."],"number":16,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1 further comprising calculating the absolute copy number of the selected protein."],"number":17,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1 wherein the biological sample comprises mutant and wild-type forms of the selected protein."],"number":18,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 18 wherein the biological sample comprises a somatic mutant form of the selected protein."],"number":19,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 18 wherein the biological sample comprises a germline mutant form of the selected protein."],"number":20,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1 wherein the step of directing output fractions employs electrospray."],"number":21,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1 wherein the step of detecting further comprises detecting transition parameters of selected product ions."],"number":22,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1 further comprising the steps of:\n
selecting precursor ions of the selected peptide representing wild type and/or mutant forms of the selected protein and the heavy-isotope labeled form of the selected peptide; and\n
fragmenting the precursor ions of the selected peptide representing wild type and/or mutant forms of the selected protein and the heavy-isotope labeled form of the selected peptide to form product ions."],"number":23,"annotation":false,"claim":true,"title":false}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}