Yeast Host Strains With Defects In N-glycosylation

Abstract

Saccharomyces mutants with defects in N-glycosylation which are obtainable by [3H]-mannose suicide selection, introduction of one or several selective markers, selection of those strains which, after transformation with the plasmid YEpL/glucose oxidase, secrete 10 mg/l glucose oxidase or more into the culture medium after culture under standard conditions, are allelic to the ngd mutations in Saccharomyces cerevisiae, DSM 7042, DSM 7338, DSM 7160 and/or 7340 and express proteins with a uniform carbohydrate structure.


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Document History
  • Publication: Aug 25, 1998
  • Application: May 31, 1996
    US US 65132396 A
  • Priority: May 31, 1996
    US US 65132396 A
  • Priority: Aug 9, 1993
    US US 10443693 A
  • Priority: Jan 25, 1993
    DE DE 4301932 A
  • Priority: Aug 7, 1992
    DE DE 4226094 A

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