{"search_session":{},"preferences":{"l":"ru","queryLanguage":"ru"},"patentId":"144-811-658-776-202","frontPageModel":{"patentViewModel":{"ref":{"entityRefType":"PATENT","entityRefId":"144-811-658-776-202"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":8635,"type":"PATENT","title":"MIT Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":30193,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8198,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Select Applicants and Owners= \"MIT\", \" Massachusetts Institute of Technology \", \"Massachus* Insti* Tech*\", \"Media Lab Massachus* Insti* Tech*\", \"Massachus* Tech* Inst*\".
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Select Applicants and Owners= \"MIT\", \" Massachusetts Institute of Technology \", \"Massachus* Insti* Tech*\", \"Media Lab Massachus* Insti* Tech*\", \"Massachus* Tech* Inst*\".
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(a) providing a reaction mixture comprising Sir2, a transcription factor, and the compound; and\n
(b) determining if the compound modulates Sir2 interaction with the transcription factor, thereby screening the compound."],"number":1,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein the Sir2 interaction with the transcription factor is direct binding, covalent modification in one or both of the Sir2 or transcription factor, a change in cellular location of the test compound, Sir2, or the transcription factor, or an alteration in activity, stability, or structure."],"number":2,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 2, wherein the determining includes comparing the binding of Sir2 to the transcription factor at a first concentration of the compound and at a second concentration of the compound."],"number":3,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 3, wherein the first or second concentration of the compound is zero."],"number":4,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein the reaction mixture further comprises a Sir2 cofactor."],"number":5,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 5, wherein the Sir2 cofactor is NAD or an NAD analog."],"number":6,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1 wherein the Sir2 is a Sir2 variant that has reduced deacetylase activity."],"number":7,"annotation":false,"title":false,"claim":true},{"lines":["The methods of claim 1, wherein the Sir2 is human."],"number":8,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 8, wherein the Sir2 is human SIRT1."],"number":9,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein the Sir2 is murine."],"number":10,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 10, wherein the Sir2 is murine Sir2α."],"number":11,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein the Sir2 is exogenous and expressed from a heterologous nucleic acid."],"number":12,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, wherein the transcription factor is exogenous and expressed from a heterologous nucleic acid."],"number":13,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 1, further comprising the step of:\n
(c) contacting or administering the compound with or to a cell or animal to evaluate the effect of the compound on the cell or animal."],"number":14,"annotation":false,"title":false,"claim":true},{"lines":["A method of screening a compound, comprising the steps of:\n
(a) providing a reaction mixture comprising Sir2, a transcription factor, and the compound; and\n
(b) determining if the compound modulates Sir2-mediated deacetylation of the transcription factor,\n
thereby screening the compound."],"number":15,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 15, wherein the determining includes comparing the acetylation status of the transcription factor, at a first concentration of the compound and at a second concentration of the compound."],"number":16,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 16, wherein the first or second concentration of the compound is zero."],"number":17,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 17, wherein the reaction mixture further comprises a Sir2 cofactor."],"number":18,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 18, wherein the Sir2 cofactor is NAD or an NAD analog."],"number":19,"annotation":false,"title":false,"claim":true},{"lines":["The method described in claim 1 wherein the transcription factor is a p53 protein or an active portion thereof."],"number":20,"annotation":false,"title":false,"claim":true},{"lines":["The method described in claim 15 wherein the transcription factor is a p53 protein or an active portion thereof."],"number":21,"annotation":false,"title":false,"claim":true},{"lines":["A method of screening a compound, comprising the steps of:\n
(a) providing a reaction mixture comprising Sir2, a transcription factor that binds a specific DNA site, and the compound; and\n
(b) determining if the compound modulates Sir2 interaction with the transcription factor,\n
thereby screening the compound."],"number":22,"annotation":false,"title":false,"claim":true},{"lines":["A method of screening a compound, comprising the steps of:\n
(a) providing a reaction mixture comprising Sir2, a transcription factor that binds a specific DNA site, and the compound; and\n
(b) determining if the compound modulates Sir2-mediated deacetylation of the transcription factor,\n
thereby screening the compound."],"number":23,"annotation":false,"title":false,"claim":true}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}