Single Cell Analysis By Polymerase Cycling Assembly

Abstract

The invention provides a method of making measurements on individual cells of a population, particularly cells that have identifying nucleic acid sequences, such as lymphoid cells. In one aspect, the invention provides a method of making multiparameter measurements on individual cells of such a population by carrying out a polymerase cycling assembly (PCA) reaction to link their identifying nucleic acid sequences to other cellular nucleic acids of interest. The fusion products of such PCA reaction are then sequenced and tabulated to generate multiparameter data for cells of the population.


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Document History
  • Publication: Aug 13, 2013
  • Application: May 4, 2011
    US US 201113100395 A
  • Priority: May 4, 2011
    US US 201113100395 A
  • Priority: Mar 14, 2011
    US US 201161452594 P
  • Priority: Feb 25, 2011
    US US 201161446822 P
  • Priority: May 6, 2010
    US US 33217510 P
  • Priority: Nov 9, 2009
    US US 61526309 A
  • Priority: Nov 7, 2008
    US US 11269308 P

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