{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"141-175-074-478-814","frontPageModel":{"patentViewModel":{"ref":{"entityRefId":"141-175-074-478-814","entityRefType":"PATENT"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":8904,"type":"PATENT","title":"Univ Washington Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":15386,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8219,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search applicants and owners= \"Univ Washington', \"Washington Univ\", \"University of Washington\", \" Washington University\", \"Univ Washington NOT state\", \" Univ Washington NOT state NOT George\".
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Total patent: 10892
Search Applicants and Owners separately : \"wash* univ* st* lou*\"; \"univ* wash* lou*\"; \"Washington University St Louis\"; \"Wash* U* St Lo*\", \" Wash* U* in St Lo*\";
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Search applicants and owners= \"Univ Washington', \"Washington Univ\", \"University of Washington\", \" Washington University\", \"Univ Washington NOT state\", \" Univ Washington NOT state NOT George\".
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Total patent: 10892
Search Applicants and Owners separately : \"wash* univ* st* lou*\"; \"univ* wash* lou*\"; \"Washington University St Louis\"; \"Wash* U* St Lo*\", \" Wash* U* in St Lo*\";
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(a) converting a rpoS-bacterium to a rpoS+ bacterium by introducing a recombiumt rpoS+ gene;\n
(b) introducing one or more inactivating mutations which render said carrier bacterium attenuated; and\n
(c) introducing a second recombinant gene encoding the desired gene product to a bacterium, wherein steps (a), (b), or (c) may be performed in any order."],"number":1,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 1 wherein the bacterium is a strain of Salmonella. "],"number":2,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 2 wherein the strain of Salmonella is a strain of S. typhi. "],"number":3,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 1 wherein the second recombinant gene encodes a gene product from a pathogen to said host."],"number":4,"annotation":false,"claim":true,"title":false},{"lines":["A method for delivery of a desired gene product to a host with a carrier bacterium, the method comprising:\n
(a) converting a rpoS− bacterium to a rpoS+ bacterium by introducing a recombinant rpoS+ gene;\n
(b) introducing one or more inactivating mutations in a bacterium which render the bacterium attenuated;\n
(c) introducing a second recombinant gene encoding the desired gene product to a bacterium, wherein steps (a), (b), or (c) may be performed in any order to yield said carrier bacterium; and\n
(d) administering to the host said carrier bacterium."],"number":5,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 5 wherein the bacterium is a strain of Salmonella. "],"number":6,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 6 wherein the strain of Salmonella is a strain of S. typhi. "],"number":7,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 5 wherein the recombinant gene encodes a product from a pathogen to said host."],"number":8,"annotation":false,"claim":true,"title":false},{"lines":["A method for delivery of a desired gene product to a host, said method comprising\n
(a) selecting for a strain of bacteria having (i) an RpoS+ phenotype, (ii) one or more inactivating mutations which render the strain attenuated, and (iii) a recombinant gene encoding the desired gene product; and\n
(b) administering the strain to the host."],"number":9,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 9 wherein the strain of bacteria is a strain of Salmonella. "],"number":10,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 10 wherein the strain of Salmonella is a strain of S. typhi. "],"number":11,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 9 wherein the recombinant gene encodes a gene product from a pathogen to said host."],"number":12,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 9 wherein the one or more inactivating mutations are in a gene selected from the group consisting of a pab gene, a pur gene, an aro gene, asd, a dap gene, nadA, pncB, galE, pmi, fur, rpsL, ompR, htrA, hemA, cdt, cya, crp, dam, phoP, phoQ, rfc, poxA, galU, metL, metH, mviA, sodC, recA, ssrA, ssrB, sirA, sirB, sirC, inv, hilA, hilC, hilD, rpoE, flgM, tonB, and slyA."],"number":13,"annotation":false,"claim":true,"title":false},{"lines":["A method for producing from a parent bacteria strain, a carrier bacteria for the delivery of a desired gene product to a host, said method comprising generating a strain of bacteria having (a) a recombinant rpoS+ gene (b) one or more inactivating mutations which render said bacteria attenuated and (c) a second recombinant gene encoding the desired gene product, wherein said carrier bacteria expresses a higher level of RpoS gene product than said parent bacteria strain and wherein said higher level of RpoS gene product confers upon the carrier bacteria high immunogenicity relative to said parent bacteria strain."],"number":14,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 14 wherein the bacteria is a strain of Salmonella. "],"number":15,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 15 wherein the Salmonella is a strain of S. typhi. "],"number":16,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 14 wherein the one or more inactivating mutations are in a gene selected from the group consisting of a pab gene, a pur gene, an aro gene, asd, a dap gene, nadA, pncB, galE, pmi, fur, rspL, ompR, htrA, hemA, cdt, cya, crp, dam, phoP, phoQ, rfc, poxA, galU, metL, metH, mviA, sodC, recA, ssrA, ssrB, sirA, sirB, sirC, inv, hilA, hilC, hilD, rpoE, flgM, tonB, and slyA."],"number":17,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 17 wherein the second recombinant gene encodes a gene product from a pathogen to said host."],"number":18,"annotation":false,"claim":true,"title":false},{"lines":["The method according to claim 18 wherein the pathogen is a virus, bacterium, protozoan, parasite or fungus."],"number":19,"annotation":false,"claim":true,"title":false},{"lines":["A method for producing a carrier bacteria for delivery of a desired gene product to a host, said method comprising selecting for a strain of bacteria having\n
(a) an RpoS+ phenotype;\n
(b) one or more inactivating mutations which render the strain attenuated; and\n
(c) a recombinant gene encoding the desired gene product."],"number":20,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 9 wherein the recombinant gene encodes an immunoregulatory molecule, allergen, or auto-antigen."],"number":21,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 21, wherein the immunoregulatory molecule is selected from the group consisting of IL-2, IL-4, IL-5, IL-6, MIF, IFN-γ, TNF-α and TGF-β."],"number":22,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 21, wherein the auto-antigen is ZP-3."],"number":23,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 14 wherein the second recombinant gene encodes a immunoregulatory molecule, allergen, or auto-antigen."],"number":24,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 24, wherein the immunoregulatory molecule is selected from the group consisting of IL-2, IL-4, IL-5, IL-6, MIF, IFN-γ, TNF-α and TGF-β."],"number":25,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 24, wherein the auto-antigen is ZP-3."],"number":26,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 20 wherein the recombinant gene encodes a immunoregulatory molecule, allergen, or auto-antigen."],"number":27,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 27, wherein the immunoregulaory molecule is selected from the group consisting of IL-2, IL-4, IL-5, IL-6, MIF, IFN-γ, TNF-α and TGF-β."],"number":28,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 27, wherein the auto-antigen is ZP-3."],"number":29,"annotation":false,"claim":true,"title":false}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}