Abstract
The present invention is directed to plants that display a pathogen resistance phenotype due to altered expression of a PPR2 nucleic acid. The invention is further directed to methods of generating plants with a pathogen resistance phenotype.
Claims
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A method of producing a plant having increased pathogen resistance comprising
identifying a plant that has an allele in a nucleotide sequence that encodes a Myb polypeptide that confers Peronospora parasitica resistance (PPR2) and that results in increased pathogen resistance compared to plants lacking the allele; and
generating progeny of the identified plant,
wherein the Myb polypeptide is selected from the group consisting of: the polypeptide sequence of SEQ ID NO: 2; a polypeptide sequence having at least 95% sequence identity to SEQ ID NO: 2; and an Arabidopsis thaliana polypeptide having at least 85% sequence identity to SEQ ID NO: 2, and wherein the generated progeny inherit the allele and have the increased pathogen resistance. - The method of claim 1 that employs Quantitative Trait Locus (QTL) methodology.
- The method of claim 1 that employs targeted induced local lesions in genomes (TILLING) methodology.
- The method of claim 1, wherein the allele alters the expression of the nucleotide sequence.
- The method of claim 1, wherein the allele alters the activity of the Myb polypeptide encoded by the nucleotide sequence.
- A plant generated by the method of claim 1.
- A plant part obtained from the plant of claim 6, wherein the plant part comprises the allele.
- The plant part of claim 7, which plant part is a seed, embryo, meristemic region, callus tissue, leaf, root, shoot, gametophyte, sporophyte, pollen, or microspore.
- The plant of claim 6, wherein the nucleotide sequence encodes an Arabidopsis thaliana polypeptide having at least 85% sequence identity to SEQ ID NO: 2.
- The plant of claim 9, wherein the nucleotide sequence encodes an Arabidopsis thaliana polypeptide having at least 90% sequence identity to SEQ ID NO: 2.
- The plant of claim 10, wherein the nucleotide sequence encodes an Arabidopsis thaliana polypeptide having at least 95% sequence identity to SEQ ID NO: 2.
- The transformed plant of claim 11, wherein the nucleotide sequence encodes SEQ ID NO: 2.
- The transformed plant of claim 12, wherein the nucleotide sequence comprises SEQ ID NO: 1.
Owners (US)
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Exelixis Plant Sciences Inc
(Oct 06 2014)
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Agrinomics Llc
(Jul 01 2005)
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Exelixis Inc
(Apr 18 2005)
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Applicants
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Agrinomics Llc
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Inventors
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Federspiel Nancy Anne
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Lammers Allan
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Liu Xing Liang
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Bates Stanley R
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Westerlund Christina
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Fitch Jonathan R
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CPC Classifications
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C12N15/8279
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C07K14/415
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C12N15/8281
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C12N15/8282
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IPC Classifications
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Document History
- Publication: Mar 1, 2011
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Application:
Mar 9, 2009
US 40054909 A
-
Priority:
Mar 9, 2009
US 40054909 A
-
Priority:
May 9, 2005
US 51260005 A
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Priority:
Apr 24, 2003
US 0312981 W
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Priority:
Apr 24, 2002
US 37533302 P