{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"137-033-856-733-834","frontPageModel":{"patentViewModel":{"ref":{"entityRefType":"PATENT","entityRefId":"137-033-856-733-834"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":11813,"type":"PATENT","title":"University of Aberdeen Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":27926,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8443,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately: univ* aberdeen. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 1658
Search Applicants and Owners separately: univ* aberdeen. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 1658
(a) a zinc finger protein comprising a non-canonical zinc finger, wherein the non-canonical zinc finger is a non-C2H2 zinc finger and has a helical portion involved in DNA binding and wherein the zinc-coordinating region of the helical portion comprises the amino acid sequence HTKICX1, and wherein the zinc finger protein is engineered to bind to a target sequence, and
(b) one or more functional domains, wherein one functional domain comprises a cleavage half-domain, and
(c) a ZC linker interposed between the cleavage half-domain and the zinc finger protein, wherein the amino acid sequence between the zinc finger protein and cleavage half-domain is denoted ZC linker."],"number":1,"annotation":false,"title":false,"claim":true},{"lines":["The fusion protein of claim 1 comprising a plurality of zinc fingers, wherein at least one of the zinc fingers comprises a CCHC zinc finger."],"number":2,"annotation":false,"title":false,"claim":true},{"lines":["The fusion protein of claim 1 or 2, wherein at least one zinc finger comprises the sequence Cys-(XA)2-4-Cys-(XB)12-His-(XC)3-5-Cys-(XD)1-10, where XA, XB, XC and XD can be any amino acid."],"number":3,"annotation":false,"title":false,"claim":true},{"lines":["The fusion protein of any of claims 1-3, comprising any of the sequences shown in any of Tables 1, 2, 3 or 4."],"number":4,"annotation":false,"title":false,"claim":true},{"lines":["The fusion protein of any of claim 3 or claim 4, wherein (XD)1-10 comprises the sequence QLV or QKP, wherein the sequence QLV or QKP are preferably the 3 C-terminal amino acid residues of the zinc finger."],"number":5,"annotation":false,"title":false,"claim":true},{"lines":["The fusion protein of any of claims 3-5, wherein (XD)1-10 comprises 1, 2 or 3 Gly (G) residues."],"number":6,"annotation":false,"title":false,"claim":true},{"lines":["The fusion protein of any one of claims 2-6, wherein the fusion protein comprises 3, 4, 5 or 6 zinc fingers."],"number":7,"annotation":false,"title":false,"claim":true},{"lines":["The fusion protein of claim 1, wherein X1 comprises G, L or T."],"number":8,"annotation":false,"title":false,"claim":true},{"lines":["The fusion protein of any of claims 2-8, wherein the zinc finger protein comprises any of the sequences shown in Table 8 and is engineered to bind to a target sequence in an IPP2-K gene."],"number":9,"annotation":false,"title":false,"claim":true},{"lines":["The fusion protein of claim 1, wherein the length of the ZC linker is 5 amino acids, preferably GLRGS (SEQ ID NO:4), or wherein the length of the ZC linker is 6 amino acids, preferably GGLRGS (SEQ ID NO:5)."],"number":10,"annotation":false,"title":false,"claim":true},{"lines":["A polynucleotide encoding a fusion protein according to any of claims 1-10."],"number":11,"annotation":false,"title":false,"claim":true},{"lines":["A method for targeted cleavage of cellular chromatin in a plant cell, the method comprising expressing, in the cell, a pair of fusion proteins according to any of claims 1-11; wherein:
(a) the target sequences of the fusion proteins are within ten nucleotides of each other; and
(b) the fusion proteins dimerize and cleave DNA located between the target sequences."],"number":12,"annotation":false,"title":false,"claim":true},{"lines":["A method of targeted genetic recombination in a host plant cell, the method comprising:
(a) expressing, in the host cell, a pair of fusion proteins according to any of claims 1-12, wherein the target sequences of the fusion proteins are present in a chosen host target locus; and
(b) identifying a recombinant host cell which exhibits a sequence alteration in the host target locus."],"number":13,"annotation":false,"title":false,"claim":true},{"lines":["The method of any of claim 12 or 13, wherein the target sequences are in an IPP2-K gene."],"number":14,"annotation":false,"title":false,"claim":true},{"lines":["A method for reducing the level of phytic acid in seeds, the method comprising inactivating or altering an IPP2-K gene according to the method of claim 14."],"number":15,"annotation":false,"title":false,"claim":true},{"lines":["A method for making phosphorous more metabolically available in seed, the method comprising inactivating or altering an IPP2-K gene according to the method of claim 14."],"number":16,"annotation":false,"title":false,"claim":true},{"lines":["A plant cell comprising a fusion protein according to any of claims 1-10, a fusion protein according to any of claims 8-10 or a polynucleotide according to claim 11."],"number":17,"annotation":false,"title":false,"claim":true}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}