{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"136-739-994-995-830","frontPageModel":{"patentViewModel":{"ref":{"entityRefId":"136-739-994-995-830","entityRefType":"PATENT"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":8819,"type":"PATENT","title":"Yale University","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":8278,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8206,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search applicants and owners = \"Yale univ\", \"Univ Yale\", \"Yale University\", \"University Yale\", \"Univ Yal*\", \"Yal* Univ*\", \"Yale Entrepreneurial Institute\".
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Total patents: 7360
Search applicants and owners = \"Yale univ\", \"Univ Yale\", \"Yale University\", \"University Yale\", \"Univ Yal*\", \"Yal* Univ*\", \"Yale Entrepreneurial Institute\".
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Total patents: 7360
(a) administering a immunogenic amount of a first Serrate protein to a host animal, which first Serrate protein (i) binds a Notch protein, and (ii) is encoded by a first nucleic acid that hybridizes under low stringency conditions to a second nucleic acid, or its complement, which second nucleic acid consists of a nucleotide sequence selected from the group consisting of the mouse Serrate sequence of SEQ ID NO:15, the antisense strand to the mouse Serrate sequence of SEQ ID NO:15, the mouse Serrate sequence of SEQ ID NO:17, the antisense strand to the mouse Serrate sequence of SEQ ID NO:17, the chick Serrate sequence depicted in FIGS. 11A-11B (SEQ ID NO:9), the antisense strand to the chick Serrate sequence depicted in FIGS. 11A-11B (SEQ ID NO:9), the human Serrate sequence depicted in FIGS. 9A-9G (SEQ ID NO:5), and the antisense strand to the human Serrate sequence depicted in FIGS. 9A-9G (SEQ ID NO:5), said low stringency conditions comprising hybridization in a buffer comprising 35% formamide, 5×SSC, 50 mM Tris-HCl (pH 7.5), 5 mM EDTA, 0.02% PVP, 0.02% Ficoll, 0.2% BSA, 100 μg/ml denatured salmon sperm DNA, and 10% (wt/vol) dextran sulfate, for 18-20 hours at 40° C., washing in a buffer consisting of 2×SSC, 25 mM Tris-HCl (pH 7.4), 5 mM EDTA, and 0.1% SDS, for 1.5 hours at 55° C., and washing in a buffer consisting of 2×SSC, 25 mM Tris-HCl (pH 7.4), 5 mM EDTA, and 0. 1% SDS, for 1.5 hours at 60° C., such that an antibody to said first Serrate protein is produced by said host animal; and
(b) recovering the antibody."],"number":18,"annotation":false,"claim":true,"title":false},{"lines":["An antibody, which does not bind a Drosophila Serrate protein, produced by a method comprising:
(a) administering a immunogenic amount of a fragment of a first Serrate protein to a host animal, in which the fragment comprises a domain of the first Serrate protein selected from the group consisting of the extracellular domain, DSL domain, domain amino-terminal to the DSL domain, epidermal growth factor-likerepeat domain, transmembrane domain, and intracellular domain, which first Serrate protein (i) binds a Notch protein, and (ii) comprises an amino acid sequence encoded by a first nucleic acid that hybridizes under low stringency conditions to a second nucleic acid, or its complement, which second nucleic acid consists of a nucleotide sequence selected from the group consisting of the mouse Serrate sequence of SEQ ID NO:15, the antisense strand to the mouse Serrate sequence of SEQ ID NO:15, the mouse Serrate sequence of SEQ ID NO:17, the antisense strand to the mouse Serrate sequence of SEQ ID NO:17, the chick Serrate sequence depicted in FIGS. 11A-11B (SEQ ID NO:9), the antisense strand to the chick Serrate sequence depicted in FIGS. 11A-11B (SEQ ID NO:9), the human Serrate sequence depicted in FIGS. 9A-9G (SEQ ID NO:5), and the antisense strand to the human Serrate sequence depicted in FIGS. 9A-9G (SEQ ID NO:5), said low stringency conditions comprising hybridization in a buffer comprising 35% formamide, 5×SSC, 50 mM Tris-HCl (pH 7.5), 5 mM EDTA, 0.02% PVP, 0.02% Ficoll, 0.2% BSA, 100 μg/ml denatured salmon sperm DNA, and 10% (wt/vol) dextran sulfate, for 18-20 hours at 40° C., washing in a buffer consisting of 2×SSC, 25 mM Tris-HCl (pH 7.4), 5 mM EDTA, and 0. 1% SDS, for 1.5 hours at 55° C., and washing in a buffer consisting of 2×SSC, 25 mM Tris-HCl (pH 7.4), 5 mM EDTA, and 0.1% SDS, for 1.5 hours at 60° C., such that an antibody to said fragment is produced by said host animal; and
(b) recovering the antibody."],"number":19,"annotation":false,"claim":true,"title":false},{"lines":["The antibody of claim 18 or 19 , in which the antibody is monoclonal."],"number":20,"annotation":false,"claim":true,"title":false},{"lines":["The antibody of claim 18 or 19 , in which the antibody is purified."],"number":21,"annotation":false,"claim":true,"title":false},{"lines":["A composition comprising an amount of an antibody of claim 18 , 19 , 20 or 21 and a pharmaceutically acceptable carrier."],"number":22,"annotation":false,"claim":true,"title":false},{"lines":["An antibody, which does not bind a Drosophila Serrate protein, produced by a method comprising:
(a) administering a immunogenic amount of a protein comprising a fragment of a first Serrate protein to a host animal, in which the fragment comprises a domain of the first Serrate protein selected from the group consisting of the extracellular domain, DSL domain, domain amino-terminal to the DSL domain, epidermal growth factor-likerepeat domain, transmembrane domain, and intracellular domain, which first Serrate protein (i) binds a Notch protein, and (ii) comprises an amino acid sequence encoded by a first nucleic acid that hybridizes under low stringency conditions to a second nucleic acid, or its complement, which second nucleic acid consists of a nucleotide sequence selected from the group consisting of the mouse Serrate sequence of SEQ ID NO:15, the antisense strand to the mouse Serrate sequence of SEQ ID NO:15, the mouse Serrate sequence of SEQ ID NO:17, the antisense strand to the mouse Serrate sequence of SEQ ID NO:17, the chick Serrate sequence depicted in FIGS. 11A-11B (SEQ ID NO:9), the antisense strand to the chick Serrate sequence depicted in FIGS. 11A-11B (SEQ ID NO:9), the human Serrate sequence depicted in FIGS. 9A-9G (SEQ ID NO:5), and the antisense strand to the human Serrate sequence depicted in FIGS. 9A-9G (SEQ ID NO:5), said low stringency conditions comprising hybridization in a buffer comprising 35% formamide, 5×SSC, 50 mM Tris-HCl (pH 7.5), 5 mM EDTA, 0.02% PVP, 0.02% Ficoll, 0.2% BSA, 100 μg/ml denatured salmon sperm DNA, and 10% (wt/vol) dextran sulfate, for 18-20 hours at 40° C., washing in a buffer consisting of 2×SSC, 25 mM Tris-HCl (pH 7.4), 5 mM EDTA, and 0.1% SDS, for 1.5 hours at 55° C., and washing in a buffer consisting of 2×SSC, 25 mM Tris-HCl (pH 7.4), 5 mM EDTA, and 0.1% SDS, for 1.5 hours at 60° C., such that an antibody to said Serrate fragment is produced by said host animal; and
(b) recovering the antibody."],"number":23,"annotation":false,"claim":true,"title":false},{"lines":["The antibody according to claim 23 , in which the fragment of the first Serrate protein is joined via a peptide bond to an amino acid sequence of a second protein, in which the second protein is not the first Serrate protein."],"number":24,"annotation":false,"claim":true,"title":false},{"lines":["A monoclonal antibody, which does not bind a Drosophila Serrate protein, produced by a method comprising:
(a) administering a immunogenic amount of a first Serrate protein to a mouse, which first Serrate protein (i) binds a Notch protein, and (ii) is encoded by a first nucleic acid that hybridizes under low stringency conditions to a second nucleic acid, or its complement, which second nucleic acid consists of a nucleotide sequence selected from the group consisting of the mouse Serrate sequence of SEQ ID NO:15, the antisense strand to the mouse Serrate sequence of SEQ ID NO:15, the mouse Serrate sequence of SEQ ID NO:17, the antisense strand to the mouse Serrate sequence of SEQ ID NO:17, the chick Serrate sequence depicted in FIGS. 11A-11B (SEQ ID NO:9), the antisense strand to the chick Serrate sequence depicted in FIGS. 11A-11B (SEQ ID NO:9), the human Serrate sequence depicted in FIGS. 9A-9G (SEQ ID NO:5), and the antisense strand to the human Serrate sequence depicted in FIGS. 9A-9G (SEQ ID NO:5), said low stringency conditions comprising hybridization in a buffer comprising 35% formamide, 5×SSC, 50 mM Tris-HCl (pH 7.5), 5 mM EDTA, 0.02% PVP, 0.02% Ficoll, 0.2% BSA, 100 μg/ml denatured salmon sperm DNA, and 10% (wt/vol) dextran sulfate, for 18-20 hours at 40° C., washing in a buffer consisting of 2×SSC, 25 mM Tris-HCl (pH 7.4), 5 mM EDTA, and 0.1% SDS, for 1.5 hours at 55° C., and washing in a buffer consisting of 2×SSC, 25 mM Tris-HCl (pH 7.4), 5 mM EDTA, and 0.1% SDS, for 1.5 hours at 60° C.;
(b) recovering spleen cells from said mouse;
(c) fusing the recovered spleen cells with a cell of a mouse myeloma to generate hybridomas;
(d) screening to select a hybridoma producing antibody to said first Serrate protein; and
(e) recovering the antibody."],"number":25,"annotation":false,"claim":true,"title":false},{"lines":["A monoclonal antibody, which does not bind a Drosophila Serrate protein, produced by a method comprising:
(a) fusing a spleen cell from a mouse immunized with an immunogenic amount of a first Serrate protein with a cell of a mouse myeloma to generate hybridomas, which first Serrate protein (i) binds a Notch protein, and (ii) is encoded by a first nucleic acid that hybridizes under low stringency conditions to a second nucleic acid, or its complement, which second nucleic acid consists of a nucleotide sequence selected from the group consisting of the mouse Serrate sequence of SEQ ID NO:15, the antisense strand to the mouse Serrate sequence of SEQ ID NO:15, the mouse Serrate sequence of SEQ ID NO:17, the antisense strand to the mouse Serrate sequence of SEQ ID NO:17, the chick Serrate sequence depicted in FIGS. 11A-11B (SEQ ID NO:9), the antisense strand to the chick Serrate sequence depicted in FIGS. 11A-11B (SEQ ID NO:9), the human Serrate sequence depicted in FIGS. 9A-9G (SEQ ID NO:5), and the antisense strand to the human Serrate sequence depicted in FIGS. 9A-9G (SEQ ID NO:5), said low stringency conditions comprising hybridization in a buffer comprising 35% formamide, 5×SSC, 50 mM Tris-HCl (pH 7.5), 5 mM EDTA, 0.02% PVP, 0.02% Ficoll, 0.2% BSA, 100 μg/ml denatured salmon sperm DNA, and 10% (wt/vol) dextran sulfate, for 18-20 hours at 40° C., washing in a buffer consisting of 2×SSC, 25 mM Tris-HCl (pH 7.4), 5 mM EDTA, and 0.1% SDS, for 1.5 hours at 55° C., and washing in a buffer consisting of 2×SSC, 25 mM Tris-HCl (pH 7.4), 5 mM EDTA, and 0. 1% SDS, for 1.5 hours at 60° C.;
(b) screening to select a hybridoma producing antibody to said first Serrate protein; and
(c) recovering the antibody."],"number":26,"annotation":false,"claim":true,"title":false},{"lines":["A monoclonal antibody, which does not bind a Drosophila Serrate protein, produced the a method comprising:
(a) administering a immunogenic amount of a first Serrate protein to a host animal, which first Serrate protein (i) binds a Notch protein, and (ii) is encoded by a first nucleic acid that hybridizes under low stringency conditions to a second nucleic acid, or its complement, which second nucleic acid consists of a nucleotide sequence selected from the group consisting of the mouse Serrate sequence of SEQ ID NO:15, the antisense strand to the mouse Serrate sequence of SEQ ID NO:15, the mouse Serrate sequence of SEQ ID NO:17, the antisense strand to the mouse Serrate sequence of SEQ ID NO:17, the chick Serrate sequence depicted in FIGS. 11A-11B (SEQ ID NO:9), the antisense strand to the chick Serrate sequence depicted in FIGS. 11A-11B (SEQ ID NO:9), the human Serrate sequence depicted in FIGS. 9A-9G (SEQ ID NO:5), and the antisense strand to the human Serrate sequence depicted in FIGS. 9A-9G (SEQ ID NO:5), said low stringency conditions comprising hybridization in a buffer comprising 35% formamide, 5×SSC, 50 mM Tris-HCl (pH 7.5), 5 mM EDTA, 0.02% PVP, 0.02% Ficoll, 0.2% BSA, 100 μg/ml denatured salmon sperm DNA, and 10% (wt/vol) dextran sulfate, for 18-20 hours at 40° C., washing in a buffer consisting of 2×SSC, 25 mM Tris-HCl (pH 7.4), 5 mM EDTA, and 0.1% SDS, for 1.5 hours at 55° C., and washing in a buffer consisting of 2×SSC, 25 mM Tris-HCl (pH 7.4), 5 mM EDTA, and 0. 1% SDS, for 1.5 hours at 60° C.;
(b) recovering lymphocytes from said host animal;
(c) fusing the recovered lymphocytes with a cell of a myeloma, plastocytoma or lymphoblastoid cell line to generate hybridomas;
(d) screening to select a hybridoma producing antibody to said first Serrate protein; and
(e) recovering the antibody."],"number":27,"annotation":false,"claim":true,"title":false},{"lines":["A monoclonal antibody, which does not bind a Drosophila Serrate protein, produced by a method comprising:
(a) fusing a lymphocyte from a host animal immunized with an immunogenic amount of a first Serrate protein with a cell of a myeloma, plastocytoma or lymphoblastoid cell line to generate hybridomas, which first Serrate protein (i) binds a Notch protein, and (ii) is encoded by a first nucleic acid that hybridizes under low stringency conditions to a second nucleic acid, or its complement, which second nucleic acid consists of a nucleotide sequence selected from the group consisting of the mouse Serrate sequence of SEQ ID NO:15, the antisense strand to the mouse Serrate sequence of SEQ ID NO:15, the mouse Serrate sequence of SEQ ID NO:17, the antisense strand to the mouse Serrate sequence of SEQ ID NO:17, the chick Serrate sequence depicted in FIGS. 11A-11B (SEQ ID NO:9), the antisense strand to the chick Serrate sequence depicted in FIGS. 11A-11B (SEQ ID NO:9), the human Serrate sequence depicted in FIGS. 9A-9G (SEQ ID NO:5), and the antisense strand to the human Serrate sequence depicted in FIGS. 9A-9G (SEQ ID NO:5), said low stringency conditions comprising hybridization in a buffer comprising 35% formamide, 5×SSC, 50 mM Tris-HCl (pH 7.5), 5 mM EDTA, 0.02% PVP, 0.02% Ficoll, 0.2% BSA, 100 μg/ml denatured salmon sperm DNA, and 10% (wt/vol) dextran sulfate, for 18-20 hours at 40° C., washing in a buffer consisting of 2×SSC, 25 mM Tris-HCl (pH 7.4), 5 mM EDTA, and 0.1% SDS, for 1.5 hours at 55° C., and washing in a buffer consisting of 2×SSC, 25 mM Tris-HCl (pH 7.4), 5 mM EDTA, and 0.1% SDS, for 1.5 hours at 60° C.;
(b) screening to select a hybridoma producing antibody to said first Serrate protein; and
(c) recovering the antibody."],"number":28,"annotation":false,"claim":true,"title":false},{"lines":["A monoclonal antibody, which does not bind a Drosophila Serrate protein, produced by a method comprising:
(a) administering a immunogenic amount of a first Serrate protein to a host animal, which first Serrate protein (i) binds a Notch protein, and (ii) is encoded by a first nucleic acid that hybridizes under low stringency conditions to a second nucleic acid, or its complement, which second nucleic acid consists of a nucleotide sequence selected from the group consisting of the mouse Serrate sequence of SEQ ID NO:15, the antisense strand to the mouse Serrate sequence of SEQ ID NO:15, the mouse Serrate sequence of SEQ ID NO:17, the antisense strand to the mouse Serrate sequence of SEQ ID NO:17, the chick Serrate sequence depicted in FIGS. 11A-11B (SEQ ID NO:9), the antisense strand to the chick Serrate sequence depicted in FIGS. 11A-11B (SEQ ID NO:9), the human Serrate sequence depicted in FIGS. 9A-9G (SEQ ID NO:5), and the antisense strand to the human Serrate sequence depicted in FIGS. 9A-9G (SEQ ID NO:5), said low stringency conditions comprising hybridization in a buffer comprising 35% formamide, 5×SSC, 50 mM Tris-HCl (pH 7.5), 5 mM EDTA, 0.02% PVP, 0.02% Ficoll, 0.2% BSA, 100 μg/ml denatured salmon sperm DNA, and 10% (wt/vol) dextran sulfate, for 18-20 hours at 40° C., washing in a buffer consisting of 2×SSC, 25 mM Tris-HCl (pH 7.4), 5 mM EDTA, and 0.1% SDS, for 1.5 hours at 55° C., and washing in a buffer consisting of 2×SSC, 25 mM Tris-HCl (pH 7.4), 5 mM EDTA, and 0.1% SDS, for 1.5 hours at 60° C.;
(b) recovering lymphocytes from said host animal;
(c) immortalizing the recovered lymphocytes with Epstein-Barr virus to generate immortalized cells;
(d) screening to select an immortalized cell producing antibody to said first Serrate protein; and
(e) recovering the antibody."],"number":29,"annotation":false,"claim":true,"title":false},{"lines":["A monoclonal antibody, which does not bind a Drosophila Serrate protein, produced by a method comprising:
(a) immortalizing a lymphocyte from a host animal immunized with an immunogenic amount of a first Serrate protein with Epstein-Barr virus to generate immortalized cells, which first Serrate protein (i) binds a Notch protein, and (ii) is encoded by a first nucleic acid that hybridizes under low stringency conditions to a second nucleic acid, or its complement, which second nucleic acid consists of a nucleotide sequence selected from the group consisting of the mouse Serrate sequence of SEQ ID NO:15, the antisense strand to the mouse Serrate sequence of SEQ ID NO:15, the mouse Serrate sequence of SEQ ID NO:17, the antisense strand to the mouse Serrate sequence of SEQ ID NO:17, the chick Serrate sequence depicted in FIGS. 11A-11B (SEQ ID NO:9), the antisense strand to the chick Serrate sequence depicted in FIGS. 11A-11B (SEQ ID NO:9), the human Serrate sequence depicted in FIGS. 9A-9G (SEQ ID NO:5), and the antisense strand to the human Serrate sequence depicted in FIGS. 9A-9G (SEQ ID NO:5), said low stringency conditions comprising hybridization in a buffer comprising 35% formamide, 5×SSC, 50 mM Tris-HCl (pH 7.5), 5 mM EDTA, 0.02% PVP, 0.02% Ficoll, 0.2% BSA, 100 μg/ml denatured salmon sperm DNA, and 10% (wt/vol) dextran sulfate, for 18-20 hours at 40° C., washing in a buffer consisting of 2×SSC, 25 mM Tris-HCl (pH 7.4), 5 mM EDTA, and 0.1% SDS, for 1.5 hours at 55° C., and washing in a buffer consisting of 2×SSC, 25 mM Tris-HCl (pH 7.4), 5 mM EDTA, and 0.1% SDS, for 1.5 hours at 60° C.;
(b) screening to select an immortalized cell producing antibody to said first Serrate protein; and
(c) recovering the antibody."],"number":30,"annotation":false,"claim":true,"title":false},{"lines":["The antibody of claim 20 , in which the monoclonal antibody is purified."],"number":31,"annotation":false,"claim":true,"title":false},{"lines":["The antibody of claim 18 or 19 , which nucleotide sequence is the human Serrate sequence depicted in FIGS. 9A-9G (SEQ ID NO:5), or the antisense strand to the human Serrate sequence depicted in FIGS. 9A-9G (SEQ ID NO:5)."],"number":32,"annotation":false,"claim":true,"title":false},{"lines":["The antibody of claim 18 or 19 , in which the first Serrate protein comprises the amino acid sequence of SEQ ID NO:6."],"number":33,"annotation":false,"claim":true,"title":false},{"lines":["The antibody of claim 18 or 19 , in which the first Serrate protein is encoded by a first nucleic acid that hybridizes under high stringency conditions to a second nucleic acid, or its complement, which second nucleic acid consists of a nucleotide sequence selected from the group consisting of the human Serrate sequence depicted in FIGS. 9A-9G (SEQ ID NO:5), and the antisense strand to the human Serrate sequence depicted in FIGS. 9A-9G (SEQ ID NO:5), said high stringency conditions comprising hybridization in a buffer comprising 6×SSC, 50 mM Tris-HCl (pH 7.5) 1 mM EDTA, 0.02% PVP, 0.02% Ficoll, 0.02% BSA and 100 μg/ml denatured salmon sperm DNA, for 48 hours at 65° C., washing in a buffer containing 2×SSC, 0.01% PVP, 0.01% Ficoll and 0.01% BSA for 1 hour at 37° C., and washing in 0.1×SSC for 45 minutes at 50° C."],"number":34,"annotation":false,"claim":true,"title":false},{"lines":["The antibody of claim 25 , 26 , 27 , 28 , 29 or 30 , which nucleotide sequence is the human Serrate sequence depicted in FIGS. 9A-9G (SEQ ID NO:5), or the antisense strand to the human Serrate sequence depicted in FIGS. 9A-9G (SEQ ID NO:5)."],"number":35,"annotation":false,"claim":true,"title":false},{"lines":["The antibody of claim 25 , 26 , 27 , 28 , 29 or 30 , in which the first Serrate protein comprises the amino acid sequence of SEQ ID NO:6."],"number":36,"annotation":false,"claim":true,"title":false},{"lines":["The antibody of claim 25 , 26 , 27 , 28 , 29 , or 30 , in which the first Serrate protein is encoded by a first nucleic acid that hybridizes under high stringency conditions to a second nucleic acid, or its complement, which second nucleic acid consists of the nucleotide sequence selected from the group consisting of the human Serrate sequence depicted in FIGS. 9A-9G (SEQ ID NO:5), and the antisense strand to the human Serrate sequence depicted in FIGS. 9A-9G (SEQ ID NO:5), said high stringency conditions comprising hybridization in a buffer comprising 6×SSC, 50 mM Tris-HCl (pH 7.5) 1 mM EDTA, 0.02% PVP, 0.02% Ficoll, 0.02% BSA and 100 μg/ml denatured salmon sperm DNA, for 48 hours at 65° C., washing in a buffer containing 2×SSC, 0.01% PVP, 0.01% Ficoll and 0.01% BSA for 1 hour at 37° C., and washing in 0.1×SSC for 45 minutes at 50° C."],"number":37,"annotation":false,"claim":true,"title":false},{"lines":["The antibody of claim 25 , 26 , 27 , 28 , 29 or 30 , in which the antibody is purified."],"number":38,"annotation":false,"claim":true,"title":false},{"lines":["A composition comprising the antibody of claim 25 , 26 , 27 , 28 , 29 or 30 , and a pharmaceutically acceptable carrier."],"number":39,"annotation":false,"claim":true,"title":false},{"lines":["A composition comprising the antibody of claim 38 , and a pharmaceutically acceptable carrier."],"number":40,"annotation":false,"claim":true,"title":false},{"lines":["An isolated antibody which binds a Serrate protein, which Serrate protein is encoded by a nucleotide sequence selected from the group consisting of the mouse Serrate sequence of SEQ ID NO:15, the antisense strand to the mouse Serrate sequence of SEQ ID NO:15, the mouse Serrate sequence of SEQ ID NO:17, the antisense strand to the mouse Serrate sequence of SEQ ID NO:17, the chick Serrate sequence depicted in FIGS. 11A-11B (SEQ ID NO:9), the antisense strand to the chick Serrate sequence depicted in FIGS. 11A-11B (SEQ ID NO:9), the human Serrate sequence depicted in FIGS. 9A-9G (SEQ ID NO:5), and the antisense strand to the human Serrate sequence depicted in FIGS. 9A-9G (SEQ ID NO:5), which antibody does not bind a Drosophila Serrate protein."],"number":41,"annotation":false,"claim":true,"title":false},{"lines":["The antibody of claim 41 , which nucleotide sequence is the human Serrate sequence depicted in FIGS. 9A-9G (SEQ ID NO:5), or the antisense strand to the human Serrate sequence depicted in FIGS. 9A-9G (SEQ ID NO:5)."],"number":42,"annotation":false,"claim":true,"title":false},{"lines":["The antibody of claim 41 or 42 which is monoclonal."],"number":43,"annotation":false,"claim":true,"title":false},{"lines":["The antibody of claim 41 or 42 which is purified."],"number":44,"annotation":false,"claim":true,"title":false},{"lines":["The antibody of claim 43 which is purified."],"number":45,"annotation":false,"claim":true,"title":false},{"lines":["A composition comprising an amount of the antibody of claim 41 or 42 , and a pharmaceutically acceptable carrier."],"number":46,"annotation":false,"claim":true,"title":false},{"lines":["An antibody, which does not bind a Drosophila Serrate protein, produced by a method comprising:
(a) administering a immunogenic amount of a Serrate protein to a host animal, which Serrate protein is encoded by a nucleotide sequence selected from the group consisting of the mouse Serrate sequence of SEQ ID NO:15, the antisense strand to the mouse Serrate sequence of SEQ ID NO:15, the mouse Serrate sequence of SEQ ID NO:17, the antisense strand to the mouse Serrate sequence of SEQ ID NO:17, the chick Serrate sequence depicted in FIGS. 11A-11B (SEQ ID NO:9), the antisense strand to the chick Serrate sequence depicted in FIGS. 11A-11B (SEQ ID NO:9), the human Serrate sequence depicted in FIGS. 9A-9G (SEQ ID NO:5), and the antisense strand to the human Serrate sequence depicted in FIGS. 9A-9G (SEQ ID NO:5), such that an antibody to said Serrate protein is produced by said host animal; and
(b) recovering the antibody."],"number":47,"annotation":false,"claim":true,"title":false},{"lines":["An antibody, which does not bind a Drosophila Serrate protein, produced by a method comprising:
(a) administering a immunogenic amount of a fragment of a Serrate protein to a host animal, in which the fragment comprises a domain of the Serrate protein selected from the group consisting of the extracellular domain, DSL domain, domain amino-terminal to the DSL domain, epidermal growth factor-likerepeat domain, transmembrane domain, and intracellular domain, which Serrate protein comprises an amino acid sequence encoded by a nucleotide sequence selected from the group consisting of the mouse Serrate sequence of SEQ ID NO:15, the antisense strand to the mouse Serrate sequence of SEQ ID NO:15, the mouse Serrate sequence of SEQ ID NO:17, the antisense strand to the mouse Serrate sequence of SEQ ID NO:17, the chick Serrate sequence depicted in FIGS. 11A-11B (SEQ ID NO:9), the antisense strand to the chick Serrate sequence depicted in FIGS. 11A-11B (SEQ ID NO:9), the human,Serrate sequence depicted in FIGS. 9A-9G (SEQ ID NO:5), and the antisense strand to the human Serrate sequence depicted in FIGS. 9A-9G (SEQ ID NO:5), such that an antibody to said fragment is produced by said host animal; and
(b) recovering the antibody."],"number":48,"annotation":false,"claim":true,"title":false},{"lines":["The antibody of claim 47 for 48 , which nucleotide sequence is the human Serrate sequence depicted in FIGS. 9A-9G (SEQ ID NO:5), or the antisense strand to the human Serrate sequence depicted in FIGS. 9A-9G (SEQ ID NO:5)."],"number":49,"annotation":false,"claim":true,"title":false},{"lines":["The antibody of claim 49 which is monoclonal."],"number":50,"annotation":false,"claim":true,"title":false},{"lines":["The antibody of claim 47 or 48 which is purified."],"number":51,"annotation":false,"claim":true,"title":false},{"lines":["The antibody of claim 50 is purified."],"number":52,"annotation":false,"claim":true,"title":false},{"lines":["An antibody, which does not bind a Drosophila Serrate protein, produced by a method comprising:
(a) administering a immunogenic amount of a protein comprising a fragment of a Serrate protein to a host animal, in which the fragment comprises a domain of the Serrate protein selected from the group consisting of the extracellular domain, DSL domain, domain amino-terminal to the DSL domain, epidermal growth factor-like repeat domain, transmembrane domain, and intracellular domain, which Serrate protein comprises an amino acid sequence encoded by a nucleotide sequence selected from the group consisting of the mouse Serrate sequence of SEQ ID NO:15, the antisense strand to the mouse Serrate sequence of SEQ ID NO:15, the mouse Serrate sequence of SEQ ID NO:17, the antisense strand to the mouse Serrate sequence of SEQ ID NO:17, the chick Serrate sequence depicted in FIGS. 11-11B (SEQ ID NO:9), the antisense strand to the chick Serrate sequence depicted in FIGS. 11A-11B (SEQ ID NO:9), the human Serrate sequence depicted in FIGS. 9A-9G (SEQ ID NO:5), and the antisense strand to the human Serrate sequence depicted in FIGS. 9A-9G (SEQ ID NO:5), such that an antibody to said Serrate fragment is produced by said host animal; and
(b) recovering the antibody."],"number":53,"annotation":false,"claim":true,"title":false},{"lines":["A monoclonal antibody, which does not bind a Drosophila Serrate protein, produced by a method comprising:
(a) administering a immunogenic amount of a Serrate protein to a mouse, which Serrate protein is encoded by a nucleotide sequence selected from the group consisting of the mouse Serrate sequence of SEQ ID NO:15, the antisense strand to the mouse Serrate sequence of SEQ ID NO 15, the mouse Serrate sequence of SEQ ID NO:17, the antisense strand to the mouse Serrate sequence of SEQ ID NO:17, the chick Serrate sequence depicted in FIGS. 11A-11B (SEQ ID NO:9), the antisense strand to the chick Serrate sequence depicted in FIGS. 11A-11B (SEQ ID NO:9), the human Serrate sequence depicted in FIGS. 9A-9G (SEQ ID NO:5), and the antisense strand to the human Serrate sequence depicted in FIGS. 9A-9G (SEQ ID NO:5);
(b) recovering spleen cells from said mouse;
(c) fusing the recovered spleen cells with a cell of a mouse myeloma to generate hybridomas;
(d) screening to select a hybridoma producing antibody to said Serrate protein; and
(e) recovering the antibody."],"number":54,"annotation":false,"claim":true,"title":false},{"lines":["A monoclonal antibody, which does not bind a Drosophila Serrate protein, produced by a method comprising:
(a) fusing a spleen cell from a mouse immunized with an immunogenic amount of a Serrate protein with a cell of a mouse myeloma to generate hybridomas, which Serrate protein is encoded by a nucleotide sequence selected from the group consisting of the mouse Serrate sequence of SEQ ID NO:15, the antisense strand to the mouse Serrate sequence of SEQ ID NO:15, the mouse Serrate sequence of SEQ ID NO:17, the antisense strand to the mouse Serrate sequence of SEQ ID NO:17, the chick Serrate sequence depicted in FIGS. 11A-11B (SEQ ID NO:9), the antisense strand to the chick Serrate sequence depicted in FIGS. 11A-11B (SEQ ID NO:9), the human Serrate sequence depicted in FIGS. 9A-9G (SEQ ID NO:5), and the antisense strand to the human Serrate sequence depicted in FIGS. 9A-9G (SEQ ID NO:5);
(b) screening to select a hybridoma producing antibody to said Serrate protein; and
(c) recovering the antibody."],"number":55,"annotation":false,"claim":true,"title":false},{"lines":["A monoclonal antibody, which does not bind a Drosophila Serrate protein, produced the a method comprising:
(a) administering a immunogenic amount of a Serrate protein to a host animal, which Serrate protein is encoded by a nucleotide sequence selected from the group consisting of the mouse Serrate sequence of SEQ ID NO:15, the antisense strand to the mouse Serrate sequence of SEQ ID NO:15, the mouse Serrate sequence of SEQ ID NO:17, the antisense strand to the mouse Serrate sequence of SEQ ID NO:17, the chick Serrate sequence depicted in FIGS. 11A-11B (SEQ ID NO:9), the antisense strand to the chick Serrate sequence depicted in FIGS. 11A-11B (SEQ ID NO:9), the human Serrate sequence depicted in FIGS. 9A-9G (SEQ ID NO:5), and the antisense strand to the human Serrate sequence depicted in FIGS. 9A-9G (SEQ ID NO:5);
(b) recovering lymphocytes from said host animal;
(c) fusing the recovered lymphocytes with a cell of a myeloma, plastocytoma or lymphoblastoid cell line to generate hybridomas;
(d) screening to select a hybridoma producing antibody to said Serrate protein; and
(e) recovering the antibody."],"number":56,"annotation":false,"claim":true,"title":false},{"lines":["A monoclonal antibody, which does not bind a Drosophila Serrate protein, produced by a method comprising:
(a) fusing a lymphocyte from a host animal immunized with an immunogenic amount of a Serrate protein with a cell of a myeloma, plastocytoma or lymphoblastoid cell line to generate hybridomas, which Serrate protein is encoded by a nucleotide sequence selected from the group consisting of the mouse Serrate sequence of SEQ ID NO:15, the antisense strand to the mouse Serrate sequence of SEQ ID NO:15, the mouse Serrate sequence of SEQ ID NO:17, the antisense strand to the mouse Serrate sequence of SEQ ID NO:17, the chick Serrate sequence depicted in FIGS. 11A-11B (SEQ ID NO:9), the antisense strand to the chick Serrate sequence depicted in FIGS. 11A-11B (SEQ ID NO:9), the human Serrate sequence depicted in FIGS. 9A-9G (SEQ ID NO:5), and the antisense strand to the human Serrate sequence depicted in FIGS. 9A-9G (SEQ ID NO:5);
(b) screening to select a hybridoma producing antibody to said Serrate protein; and
(c) recovering the antibody."],"number":57,"annotation":false,"claim":true,"title":false},{"lines":["A monoclonal antibody, which does not bind a Drosophila Serrate protein, produced by a method comprising:
(a) administering a immunogenic amount of a Serrate protein to a host animal, which Serrate protein is encoded by a nucleotide sequence selected from the group consisting of the mouse Serrate sequence of SEQ ID NO:15, the antisense strand to the mouse Serrate sequence of SEQ ID NO:15, the mouse Serrate sequence of SEQ ID NO:17, the antisense strand to the mouse Serrate sequence of SEQ ID NO:17, the chick Serrate sequence depicted in FIGS. 11A-11B (SEQ ID NO:9), the antisense strand to the chick Serrate sequence depicted in FIGS. 11A-11B (SEQ ID NO:9), the human Serrate sequence depicted in FIGS. 9A-9G (SEQ ID NO:5), and the antisense strand to the human Serrate sequence depicted in FIGS. 9A-9G (SEQ ID NO:5);
(b) recovering lymphocytes from said host animal;
(c) immortalizing the recovered lymphocytes with Epstein-Barr virus to generate immortalized cells;
(d) screening to select an immortalized cell producing antibody to said Serrate protein; and
(e) recovering the antibody."],"number":58,"annotation":false,"claim":true,"title":false},{"lines":["A monoclonal antibody, which does not bind a Drosophila Serrate protein, produced by a method comprising:
(a) immortalizing a lymphocyte from a host animal immunized with an immunogenic amount of a Serrate protein with Epstein-Barr virus to generate immortalized cells, which Serrate protein is encoded by a nucleotide sequence selected from the group consisting of the mouse Serrate sequence of SEQ ID NO:15, the antisense strand to the mouse Serrate sequence of SEQ ID NO:15, the mouse Serrate sequence of SEQ ID NO:17, the antisense strand to the mouse Serrate sequence of SEQ ID NO:1 7, the chick Serrate sequence depicted in FIGS. 11A-11B (SEQ ID NO:9), the antisense strand to the chick Serrate sequence depicted in FIGS. 11A-11B (SEQ ID NO:9), the human Serrate sequence depicted in FIGS. 9A-9G (SEQ ID NO:5), and the antisense strand to the human Serrate sequence depicted in FIGS. 9A-9G (SEQ ID NO:5);
(b) screening to select an immortalized cell producing antibody to said Serrate protein; and
(c) recovering the antibody."],"number":59,"annotation":false,"claim":true,"title":false},{"lines":["The antibody of claim 53 , 54 , 55 , 56 , 57 , 58 or 59 , which nucleotide sequence is the human Serrate sequence depicted in FIGS. 9A-9G (SEQ ID NO:5), or the antisense strand to the human Serrate sequence depicted in FIGS. 9A-9G (SEQ ID NO:5)."],"number":60,"annotation":false,"claim":true,"title":false},{"lines":["The antibody of claim 60 which is purified."],"number":61,"annotation":false,"claim":true,"title":false},{"lines":["A composition comprising an amount of an antibody of claim 60 ; and a pharmaceutically acceptable carrier."],"number":62,"annotation":false,"claim":true,"title":false},{"lines":["An insolated antibody which binds a Serrate protein, which Serrate protein consists of the amino acid sequence of SEQ ID NO:6, SEQ ID NO:10, SEQ ID NO:16 or SEQ ID NO:18."],"number":63,"annotation":false,"claim":true,"title":false},{"lines":["The antibody of claim 63 , which Serrate protein consists of the amino acid sequence of SEQ ID NO:6."],"number":64,"annotation":false,"claim":true,"title":false},{"lines":["The antibody of claim 63 or 64 which is purified."],"number":65,"annotation":false,"claim":true,"title":false},{"lines":["The antibody of claim 63 or 64 which is monoclonal."],"number":66,"annotation":false,"claim":true,"title":false},{"lines":["An antibody, which does not bind a Drosophila Serrate protein, produced by a method comprising:
(a) administering a immunogenic amount of a Serrate protein to a host animal, which Serrate protein consists of the amino acid sequence of SEQ ID NO:6, SEQ ID NO:10, SEQ ID NO:16 or SEQ ID NO:18, such that an antibody to said Serrate protein is produced by said host animal; and
(b) recovering the antibody."],"number":67,"annotation":false,"claim":true,"title":false},{"lines":["An antibody, which does not bind a Drosophila Serrate protein, produced by a method comprising:
(a) administering a immunogenic amount of a fragment of a Serrate protein to a host animal, in which the fragment comprises a domain of the Serrate protein selected from the group consisting of the extracellular domain, DSL domain, domain amino-terminal to the DSL domain, epidermal growth factor-likerepeat domain, transmembrane domain, and intracellular domain, which Serrate protein consists of the amino acid sequence of SEQ ID NO:6, SEQ ID NO:10, SEQ ID NO:16 or SEQ ID NO:18, such that an antibody to said fragment is produced by said host animal; and
(b) recovering the antibody."],"number":68,"annotation":false,"claim":true,"title":false},{"lines":["The antibody of claim 67 or 68 , which Serrate protein consists of the amino acid sequence of SEQ ID NO:6."],"number":69,"annotation":false,"claim":true,"title":false},{"lines":["An antibody, which does not bind a Drosophila Serrate protein, produced by a method comprising:
(a) administering a immunogenic amount of a protein comprising a fragment of a Serrate protein to a host animal, in which the fragment comprises a domain of the Serrate protein selected from the group consisting of the extracellular domain, DSL domain, domain amino-terminal to the DSL domain, epidermal growth factor-likerepeat domain, transmembrane domain, and intracellular domain, which Serrate protein consists of the amino acid sequence of SEQ ID NO:6, SEQ ID NO:10, SEQ ID NO:16 or SEQ ID NO:18, such that an antibody to said Serrate fragment is produced by said host animal; and
(b) recovering the antibody."],"number":70,"annotation":false,"claim":true,"title":false},{"lines":["A monoclonal antibody, which does not bind a Drosophila Serrate protein, produced by a method comprising:
(a) fusing a spleen cell from a mouse immunized with an immunogenic amount of a Serrate protein with a cell of a mouse myeloma to generate hybridomas, which Serrate protein consists of the amino acid sequence of SEQ ID NO:6, SEQ ID NO: 10, SEQ ID NO:16 or SEQ ID NO:18;
(b) screening to select a hybridoma producing antibody to said Serrate protein; and
(c) recovering the antibody."],"number":71,"annotation":false,"claim":true,"title":false},{"lines":["The antibody of claim 70 or 71 , which Serrate protein consists of the amino acid sequence of SEQ ID NO:6."],"number":72,"annotation":false,"claim":true,"title":false},{"lines":["The antibody of claim 67 , 68 , 69 , 70 or 71 which is purified."],"number":73,"annotation":false,"claim":true,"title":false},{"lines":["A fragment or derivative of the antibody of claim 41 , 42 , 47 , 48 , 53 , 54 , 55 , 56 , 57 , 58 or 59 , which fragment or derivative binds said Serrate protein."],"number":74,"annotation":false,"claim":true,"title":false},{"lines":["A fragment or derivative of the antibody of claim 67 , 68 , 70 or 71 , which fragment or derivative binds said Serrate protein."],"number":75,"annotation":false,"claim":true,"title":false}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}