{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"130-743-896-294-559","frontPageModel":{"patentViewModel":{"ref":{"entityRefId":"130-743-896-294-559","entityRefType":"PATENT"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":8902,"type":"PATENT","title":"University of Tokyo Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":20983,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8217,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search applicants and owners = \"Univ Tokyo\", \"Univ Tokyo NOT metropolitan\", \"Tokyo Univ\",\"University of Tokyo NOT metropolitan\", \"Tokyo Univ\".
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Total patent: 13934
Search applicants and owners = \"Univ Tokyo\", \"Univ Tokyo NOT metropolitan\", \"Tokyo Univ\",\"University of Tokyo NOT metropolitan\", \"Tokyo Univ\".
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Total patent: 13934
wherein R1 is —CN, —CONH2, —CH═CH—CN: or —CH═CH—CONH2,\n
R2 is a C 1-4 alkyl group,\n
R3, and R4 are each independently a hydrogen atom, a halogen atom or a C 1-4 alkyl group, and\n
R5 is a hydrogen atom, a C 1-4 alkylcarbonyl group, or a C 1-4 alkylcarbonyloxymethyl group."],"number":1,"annotation":false,"claim":true,"title":false},{"lines":["A fluorescent substrate, according to claim 1, wherein the substrate is suitable for detecting an enzymatic activity of a nitrile-related enzyme wherein the nitrile-related enzyme is selected from the group consisting of nitrilase, nitrile hydratase, and amidase."],"number":2,"annotation":false,"claim":true,"title":false},{"lines":["The substrate of claim 2, wherein R1 in formula (I) is —CN or —CH═CH—CN, and the nitrile-related enzyme is nitrilase or nitrile hydratase."],"number":3,"annotation":false,"claim":true,"title":false},{"lines":["The substrate of claim 2, wherein R1 in formula (I) is —CONH2 or —CH═CH—CONH2, and the nitrile-related enzyme is amidase."],"number":4,"annotation":false,"claim":true,"title":false},{"lines":["A kit comprising the compound of claim 1."],"number":5,"annotation":false,"claim":true,"title":false},{"lines":["The kit of claim 5, further comprising a cell lysis solution, a buffer and/or instructions for use."],"number":6,"annotation":false,"claim":true,"title":false},{"lines":["A method for detecting enzymatic activity of a nitrile-related enzyme, comprising:\n
(a) contacting the nitrile-related enzyme with the compound of claim 1, wherein the nitrile-related enzyme is selected from the group consisting of nitrilase, nitrile hydratase, and amidase and\n
(b) measuring the optical intensity of an emitted wavelength generated as a result of step (a), wherein an increase in the intensity of the emitted wavelength positively correlates to nitrile-related enzymatic activity."],"number":7,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 7, wherein R1 in formula (I) is —CN or —CH═CH—CN, and the nitrile-related enzyme is nitrilase or nitrile hydratase."],"number":8,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 7, wherein R1 in formula (I) is —CONH2 or —CH═CH—CONH2, and the nitrile-related enzyme is amidase."],"number":9,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 7, wherein measuring step (b) comprises flow cytometry."],"number":10,"annotation":false,"claim":true,"title":false}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}