{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"129-394-336-210-240","frontPageModel":{"patentViewModel":{"ref":{"entityRefType":"PATENT","entityRefId":"129-394-336-210-240"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":10810,"type":"PATENT","title":"University of Florida - Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":12320,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8273,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately: \"Univ* Florida\"
Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 1000+
Search Applicants and Owners separately:univ* AND Montpellier. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 1289
Search Applicants and Owners separately: \"Univ* Florida\"
Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 1000+
Search Applicants and Owners separately:univ* AND Montpellier. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 1289
a) a nucleic acid molecule comprising a nucleotide sequence which is at least 60% homologous to the nucleotide sequence of SEQ ID NO: 1 or SEQ ID NO: 3, or a complement thereof;\n
b) a nucleic acid molecule comprising a fragment of at least 100 nucleotides of a nucleic acid comprising the nucleotide sequence of SEQ ID NO: 1 or SEQ ID NO: 3, or a complement thereof;\n
c) a nucleic acid molecule which encodes a polypeptide comprising an amino acid sequence at least about 50% homologous to the amino acid sequence of SEQ ID NO: 2 or SEQ ID NO: 4;\n
d) a nucleic acid molecule which encodes a fragment of a polypeptide comprising the amino acid sequence of SEQ ID NO: 2 or SEQ ID NO: 4; wherein the fragment comprises at least 15 contiguous amino acid residues of the amino acid sequence of SEQ ID NO: 2 or SEQ ID NO: 4;\n
e) a nucleic acid which encodes a naturally occurring allelic variant of a polypeptide comprising the amino acid sequence of SEQ ID NO: 2 or SEQ ID NO: 4, wherein the nucleic acid molecule hybridizes to a complement of a nucleic acid molecule comprising SEQ ID NO: 1 or SEQ ID NO: 3, under stringent conditions;\n
f) a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 1 or SEQ ID NO: 3, or a complement thereof; and\n
g) a nucleic acid molecule which encodes a polypeptide comprising the amino acid sequence of SEQ ID NO: 2 or SEQ ID NO: 4;\nwherein the nucleic acid molecule when expressed in a cell, renders the cell capable of producing ethanol as the primary fermentation product."],"number":18,"annotation":false,"title":false,"claim":true},{"lines":["19-26. (canceled)"],"number":-1,"annotation":false,"title":false,"claim":true},{"lines":["An isolated polypeptide selected from the group consisting of:\n
a) a fragment of a polypeptide comprising the amino acid sequence of SEQ ID NO; 2 or SEQ ID NO: 4, wherein the fragment comprises at least 15 contiguous amino acids of SEQ ID NO: 2 or SEQ ID NO: 4;\n
b) a naturally occurring allelic variant of a polypeptide comprising the amino acid sequence of SEQ ID NO; 2 or SEQ ID NO: 4, wherein the polypeptide is encoded by a nucleic acid molecule which hybridizes to the complement of a nucleic acid molecule comprising SEQ ID NO; 1 or SEQ ID NO: 3, under stringent conditions;\n
c) a polypeptide which is encoded by a nucleic acid molecule which is at least 50% identical to a nucleic acid comprising the nucleotide sequence of SEQ ID NO: 1 or SEQ ID NO: 3;\n
d) a polypeptide comprising an amino acid sequence which is at least 90% identical to the amino acid sequence of SEQ ID NO: 2 or SEQ ID NO: 4; and\n
e) an isolated polypeptide comprising the amino acid sequence of SEQ ID NO: 2 or SEQ ID NO: 4;\nwherein the polypeptide when expressed in a cell, renders the cell capable of producing ethanol as the primary fermentation product."],"number":27,"annotation":false,"title":false,"claim":true},{"lines":["28-35. (canceled)"],"number":-1,"annotation":false,"title":false,"claim":true},{"lines":["A bacterial host cell comprising the nucleic acid molecule claim[s] 18[-26]."],"number":36,"annotation":false,"title":false,"claim":true},{"lines":["37-39. (canceled)"],"number":-1,"annotation":false,"title":false,"claim":true},{"lines":["A bacterial host cell comprising the polypeptide of claim[s] 27[-35]."],"number":40,"annotation":false,"title":false,"claim":true},{"lines":["(canceled)"],"number":41,"annotation":false,"title":false,"claim":true},{"lines":["(canceled)"],"number":42,"annotation":false,"title":false,"claim":true},{"lines":["The isolated non-recombinant bacterium of claim[s 1-4]5, wherein ethanol is produced from the sugar."],"number":43,"annotation":false,"title":false,"claim":true},{"lines":["The isolated non-recombinant bacterium of claim [42 or]43, wherein the sugar is selected from the group consisting of glucose, xylose, arabinose, mannose, galactose, sucrose, and lactose."],"number":44,"annotation":false,"title":false,"claim":true},{"lines":["The isolated non-recombinant bacterium of claim[s] 1[-7], wherein the bacterium is selected from the group consisting of Gram-negative bacteria and Gram-positive bacteria."],"number":45,"annotation":false,"title":false,"claim":true},{"lines":["The isolated non-recombinant bacterium of claim[s 1-7]45, wherein the bacterium is a Gram-negative bacterium."],"number":46,"annotation":false,"title":false,"claim":true},{"lines":["The isolated non-recombinant bacterium of claim[s 1-7]46, wherein the Gram-negative bacterium is selected from the group consisting of Acinetobacter, Gluconobacter, Escherichia, Geobacter, Shewanella, Salmonella, Enterobacter and Klebsiella. "],"number":47,"annotation":false,"title":false,"claim":true},{"lines":["The isolated non-recombinant bacterium of claim[s 1-7]45, wherein the bacterium is a Gram-positive bacterium."],"number":48,"annotation":false,"title":false,"claim":true},{"lines":["The isolated non-recombinant bacterium of claim[s 1-7]48, wherein the Gram-positive bacterium is selected from the group consisting of Bacillus, Clostridium, Corynebacterium, Lactobacillis, Lactococcus, Oenococcus, Streptococcus and Eubacterium. "],"number":49,"annotation":false,"title":false,"claim":true},{"lines":["The isolated non-recombinant bacterium of claim[s 1-7]47, wherein the bacterium is Escherichia coli. "],"number":50,"annotation":false,"title":false,"claim":true},{"lines":["The isolated non-recombinant bacterium of claim 4, wherein the mutation comprises substitution of an amino acid with another amino acid in a polypeptide expressed by the mutated lpd gene, wherein the substitution changes the pK of the polypeptide."],"number":51,"annotation":false,"title":false,"claim":true},{"lines":["The isolated non-recombinant bacterium of claim 51, wherein the polpypeptide comprises SEQ ID NO: 6 and the mutation comprises a substitution of a wild type amino acid with another amino acid at:\n
a) position 322 or any position within about 50 positions on either side of position 322 in SEQ ID NO: 6; or b) position 354 or any position within about 50 positions on either side of position 354 in SEQ ID NO: 6."],"number":52,"annotation":false,"title":false,"claim":true},{"lines":["The isolated non-recombinant bacterium of claim[s] 51, wherein the another amino acid is a neutral amino acid selected from the group consisting of alanine, cysteine, glycine, isoleucine, leucine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine, and valine."],"number":53,"annotation":false,"title":false,"claim":true},{"lines":["The isolated non-recombinant bacterium of claim[s] 51, wherein the another amino acid is a basic amino acid selected from the group consisting of arginine, asparagine, glutamine, histidine and lysine."],"number":54,"annotation":false,"title":false,"claim":true},{"lines":["The isolated non-recombinant bacterium of 52, wherein the mutation comprises a substitution of H at position 322 with any amino acid, such that the amino acid substitution increases the acidity of the polypeptide expressed by the mutated lpd gene."],"number":55,"annotation":false,"title":false,"claim":true},{"lines":["The isolated non-recombinant bacterium of claim 55, wherein the mutation comprises a substitution of H to Y at position 322 in SEQ ID NO: 6."],"number":56,"annotation":false,"title":false,"claim":true},{"lines":["The isolated non-recombinant bacterium of 52, wherein the mutation comprises a substitution of E at position 354 with any amino acid, such that the amino acid substitution reduces the acidity of the polypeptide expressed by the mutated lpd gene."],"number":57,"annotation":false,"title":false,"claim":true},{"lines":["The isolated non-recombinant bacterium of claim 57, wherein the mutation comprises a substitution of E to K at position 354 in SEQ ID NO: 6."],"number":58,"annotation":false,"title":false,"claim":true},{"lines":["The isolated non-recombinant bacterium of claim 56, wherein the bacterium is E. coli strain SE2377."],"number":59,"annotation":false,"title":false,"claim":true},{"lines":["The isolated non-recombinant bacterium of claim 59, wherein the bacterium comprises SEQ ID NO: 1, or a fragment thereof."],"number":60,"annotation":false,"title":false,"claim":true},{"lines":["The isolated non-recombinant bacterium of claim 58, wherein the bacterium is E. coli strain SE2378."],"number":61,"annotation":false,"title":false,"claim":true},{"lines":["The isolated non-recombinant bacterium of claim 61, wherein the bacterium comprises SEQ ID NO: 3, or a fragment thereof."],"number":62,"annotation":false,"title":false,"claim":true},{"lines":["The isolated non-recombinant bacterium of claim 58, wherein the bacterium is E. coli strain SE2382."],"number":63,"annotation":false,"title":false,"claim":true},{"lines":["The isolated non-recombinant bacterium of claim 63, wherein the bacterium comprises SEQ ID NO: 3, or a fragment thereof."],"number":64,"annotation":false,"title":false,"claim":true},{"lines":["The isolated non-recombinant bacterium of claim 56, wherein the bacterium is E. coli strain SE2383."],"number":65,"annotation":false,"title":false,"claim":true},{"lines":["The isolated non-recombinant bacterium of claim 65, wherein the bacterium comprises SEQ ID NO: 1, or a fragment thereof."],"number":66,"annotation":false,"title":false,"claim":true},{"lines":["The isolated non-recombinant bacterium of claim 56, wherein the bacterium is E. coli strain SE2384."],"number":67,"annotation":false,"title":false,"claim":true},{"lines":["The isolated non-recombinant bacterium of claim 67, wherein the bacterium comprises SEQ ID NO: 1, or a fragment thereof."],"number":68,"annotation":false,"title":false,"claim":true},{"lines":["The isolated non-recombinant bacterium of claim 58, wherein the bacterium is E. coli strain SE2385."],"number":69,"annotation":false,"title":false,"claim":true},{"lines":["The isolated non-recombinant bacterium of claim 69, wherein the bacterium comprises SEQ ID NO: 3, or a fragment thereof."],"number":70,"annotation":false,"title":false,"claim":true},{"lines":["The isolated non-recombinant bacterium of claim[s] 56[-70], wherein the bacterium is suitable for producing ethanol from sugar."],"number":71,"annotation":false,"title":false,"claim":true},{"lines":["An isolated non-recombinant bacterium comprising a lpd gene having one or more mutations, wherein the mutation renders the non-recombinant bacterium capable of producing ethanol as the primary fermentation product under anaerobic conditions, wherein the bacterium is prepared by a process comprising the steps of:\n
a) growing a candidate mutant strain of the bacterium under anaerobic growth conditions in sugar-rich medium; and\n
b) selecting mutants that produce ethanol as the major product of fermentation."],"number":72,"annotation":false,"title":false,"claim":true},{"lines":["(canceled)"],"number":73,"annotation":false,"title":false,"claim":true},{"lines":["A method of producing the non-recombinant bacterium of claim[s] 1[-8], comprising the steps of:\n
a) growing a candidate mutant strain of the bacterium under anaerobic growth conditions in sugar-rich medium; and\n
b) selecting mutants that produce ethanol as the major product of fermentation."],"number":74,"annotation":false,"title":false,"claim":true},{"lines":["75-90. (canceled)"],"number":-1,"annotation":false,"title":false,"claim":true},{"lines":["A method for producing ethanol from an oligosaccharide source comprising, contacting the oligosaccharide with the isolated non-recombinant bacterium of claim[s] 1[-8] or the bacterial host cell of claim 36, thereby producing ethanol from an oligosaccharide source."],"number":91,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 91 wherein the oligosaccharide is selected from the group consisting of lignocellulose, hemicellulose, cellulose, pectin and any combination thereof."],"number":92,"annotation":false,"title":false,"claim":true},{"lines":["A kit comprising the isolated non-recombinant bacterium of claim[s] 1[-8] and instructions for producing ethanol."],"number":93,"annotation":false,"title":false,"claim":true},{"lines":["(canceled)"],"number":94,"annotation":false,"title":false,"claim":true},{"lines":["The E. coli strain AH218 represented by a deposit with the Agricultural Research Culture Collection designated as deposit number NRRL B-30967."],"number":95,"annotation":false,"title":false,"claim":true},{"lines":["The E. coli strain AH241 represented by a deposit with the Agricultural Research Culture Collection designated as deposit number NRRL B-30968."],"number":96,"annotation":false,"title":false,"claim":true},{"lines":["The E. coli strain AH242 represented by a deposit with the Agricultural Research Culture Collection designated as deposit number NRRL B-30969."],"number":97,"annotation":false,"title":false,"claim":true},{"lines":["The E. coli strain SE2377 represented by a deposit with the Agricultural Research Culture Collection fdesignated as deposit number NRRL B-30970."],"number":98,"annotation":false,"title":false,"claim":true},{"lines":["The E. coli strain SE2378 represented by a deposit with the Agricultural Research Culture Collection designated as deposit number NRRL B-30971."],"number":99,"annotation":false,"title":false,"claim":true},{"lines":["The E. coli strain SE2382 represented by a deposit with the Agricultural Research Culture Collection designated as deposit number NRRL B-30972."],"number":100,"annotation":false,"title":false,"claim":true},{"lines":["The E. coli strain SE2383 represented by a deposit with the Agricultural Research Culture Collection designated as deposit number NRRL B-30973."],"number":101,"annotation":false,"title":false,"claim":true},{"lines":["The E. coli strain SE2384 represented by a deposit with the Agricultural Research Culture Collection designated as deposit number NRRL B-30974."],"number":102,"annotation":false,"title":false,"claim":true},{"lines":["The E. coli strain SE2385 represented by a deposit with the Agricultural Research Culture Collection designated as deposit number NRRL B-30975."],"number":103,"annotation":false,"title":false,"claim":true},{"lines":["The isolated non-recombinant bacterium of claim 4, wherein the mutation in the lpd gene causes NADH insensitivity."],"number":104,"annotation":false,"title":false,"claim":true},{"lines":["The method of claim 91, wherein the mutant results from mutation in the lpd gene."],"number":105,"annotation":false,"title":false,"claim":true},{"lines":["The method claim 105, wherein mutation in the lpd gene causes NADH insensitivity."],"number":106,"annotation":false,"title":false,"claim":true}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}