{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"120-274-492-045-378","frontPageModel":{"patentViewModel":{"ref":{"entityRefId":"120-274-492-045-378","entityRefType":"PATENT"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":11724,"type":"PATENT","title":"University of Miami - Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":2822,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8376,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately: uni* Miami
Select more for logical variants.
Add to collection.
Select all patents in the collection and expand by simple families.
Add to collection. Total patents: 2802
Search Applicants and Owners separately: uni* Miami
Select more for logical variants.
Add to collection.
Select all patents in the collection and expand by simple families.
Add to collection. Total patents: 2802
(a) initially impregnating a tissue specimen with a single non-aqueous admixture comprised of (i) fixing, (ii) dehydrating, and (iii) clearing functions which is heated; and then\n
(b) substantially impregnating the tissue specimen with a single wax solution which is heated and also under less than atmospheric pressure."],"number":35,"annotation":false,"claim":true,"title":false},{"lines":["The tissue processing method of claim 35, wherein the tissue specimen is about 3 mm or less in thickness."],"number":36,"annotation":false,"claim":true,"title":false},{"lines":["The tissue processing method of claim 35, wherein tissue specimens are manually processed."],"number":37,"annotation":false,"claim":true,"title":false},{"lines":["The tissue processing method of claim 35, wherein tissue specimens are mechanically processed."],"number":38,"annotation":false,"claim":true,"title":false},{"lines":["The tissue processing method of claim 35, wherein the tissue specimen is processed without using an aldehyde."],"number":39,"annotation":false,"claim":true,"title":false},{"lines":["The tissue processing method of claim 35, wherein the tissue specimen is processed without using a xylene."],"number":40,"annotation":false,"claim":true,"title":false},{"lines":["The tissue processing method of claim 35, wherein the tissue specimen has not been fixed prior to processing."],"number":41,"annotation":false,"claim":true,"title":false},{"lines":["The tissue processing method of claim 35, wherein the tissue specimen has been fixed in formaldehyde prior to processing."],"number":42,"annotation":false,"claim":true,"title":false},{"lines":["The tissue processing method of claim 35, wherein the tissue specimen has been preserved in methanol and polyethylene glycol (PEG) prior to processing."],"number":43,"annotation":false,"claim":true,"title":false},{"lines":["The tissue processing method of claim 35, wherein the tissue specimen is processed in a microwave retort for (a)."],"number":44,"annotation":false,"claim":true,"title":false},{"lines":["The tissue processing method of claim 44, wherein the tissue specimen is substantially hardened by the non-aqueous admixture, radiation from the microwave unit, or both."],"number":45,"annotation":false,"claim":true,"title":false},{"lines":["The tissue processing method of claim 35 further comprising agitation to promote chemical exchange between the non-aqueous admixture and the tissue specimen."],"number":46,"annotation":false,"claim":true,"title":false},{"lines":["The tissue processing method of claim 35, wherein the non-aqueous admixture is above about 55° C. and/or below about 75° C."],"number":47,"annotation":false,"claim":true,"title":false},{"lines":["The tissue processing method of claim 35, wherein the non-aqueous admixture is comprised of fixative and dehydrant in a volume ratio greater than about 1:6 and/or less than about 6:1."],"number":48,"annotation":false,"claim":true,"title":false},{"lines":["The tissue processing method of claim 35, wherein a fixative is greater than about 20% (v/v) and/or less than about 60% (v/v) of the non-aqueous admixture."],"number":49,"annotation":false,"claim":true,"title":false},{"lines":["The tissue processing method of claim 35, wherein a dehydrant is greater than about 20% (v/v) and/or less than about 60% (v/v) of the non-aqueous admixture."],"number":50,"annotation":false,"claim":true,"title":false},{"lines":["The tissue processing method of claim 35, wherein a clearant is greater than about 10% (v/v) and/or less than about 30% (v/v) of the non-aqueous admixture."],"number":51,"annotation":false,"claim":true,"title":false},{"lines":["The tissue processing method of claim 35, wherein the non-aqueous admixture is further comprised of a surfactant."],"number":52,"annotation":false,"claim":true,"title":false},{"lines":["The tissue processing method of claim 35, wherein the non-aqueous admixture is comprised of a fixative which is at least one ketone and a dehydrant which is at least one alcohol."],"number":53,"annotation":false,"claim":true,"title":false},{"lines":["The tissue processing method of claim 35, wherein the non-aqueous admixture is comprised of a clearant which is mineral oil."],"number":54,"annotation":false,"claim":true,"title":false},{"lines":["The tissue processing method of claim 35, wherein the non-aqueous admixture is further comprised of dimethyl sulfoxide (DMSO)."],"number":55,"annotation":false,"claim":true,"title":false},{"lines":["The tissue processing method of claim 35, wherein the non-aqueous admixture is comprised of about 15% (v/v) to about 35% (v/v) acetone, about 45% (v/v) to about 65% (v/v) isopropyl alcohol, and about 10% (v/v) to about 25% (v/v) mineral oil."],"number":56,"annotation":false,"claim":true,"title":false},{"lines":["The tissue processing method of claim 35, wherein the non-aqueous admixture is comprised of about 40% to about 60% (v/v) of acetone, about 25% to about 35% (v/v) of isopropanol, and about 10% (v/v) to about 25% (v/v) of mineral oil."],"number":57,"annotation":false,"claim":true,"title":false},{"lines":["The tissue processing method of claim 35, wherein the tissue specimen is substantially hardened after being incubated in the non-aqueous admixture for about 15 minutes or less."],"number":58,"annotation":false,"claim":true,"title":false},{"lines":["The tissue processing method of claim 35, wherein the tissue specimen is substantially hardened after being incubated in the non-aqueous admixture for about 30 minutes or less."],"number":59,"annotation":false,"claim":true,"title":false},{"lines":["The tissue processing method of claim 35, wherein the tissue specimen is substantially hardened after being incubated in the non-aqueous admixture for about 45 minutes or less."],"number":60,"annotation":false,"claim":true,"title":false},{"lines":["The tissue processing method of claim 35, wherein the tissue specimen is processed in a vacuum retort for (b)."],"number":61,"annotation":false,"claim":true,"title":false},{"lines":["The tissue processing method of claim 61, wherein the tissue specimen is substantially impregnated at a pressure above about 100 torr and/or below about 760 torr."],"number":62,"annotation":false,"claim":true,"title":false},{"lines":["The tissue processing method of claim 35, wherein the wax solution is above about 55° C. and/or below about 85° C."],"number":63,"annotation":false,"claim":true,"title":false},{"lines":["The tissue processing method of claim 35, wherein the wax solution has been degassed and dehydrated."],"number":64,"annotation":false,"claim":true,"title":false},{"lines":["The tissue processing method of claim 35, wherein the wax solution is made from paraffin which is solid below about 30° C."],"number":65,"annotation":false,"claim":true,"title":false},{"lines":["The tissue processing method of claim 35, wherein the tissue specimen can be sectioned after being incubated in the wax solution for about 15 minutes or less, and then cooled."],"number":66,"annotation":false,"claim":true,"title":false},{"lines":["The tissue processing method of claim 35, wherein the tissue specimen can be sectioned after being incubated in the wax solution for about 30 minutes or less, and then cooled."],"number":67,"annotation":false,"claim":true,"title":false},{"lines":["The tissue processing method of claim 35, wherein the tissue specimen can be sectioned after being incubated in the wax solution for about 45 minutes or less, and then cooled."],"number":68,"annotation":false,"claim":true,"title":false},{"lines":["A method for tissue processing of one or more tissue specimens using only two different chemical compositions and an apparatus comprising:\n
a first module comprising (i) a first reaction chamber with an interior geometry comprised of a whispering gallery to achieve uniform distribution of microwave energy and heating of the interior, (ii) a single non-aqueous admixture which is optionally contained within the first reaction chamber, (iii) a source of microwave energy which is transmitted into the first reaction chamber to heat the non-aqueous admixture, and (iv) an agitator within the first reaction chamber to promote chemical exchange between the non-aqueous admixture and the tissue specimen and\n
a second module comprising (i) a second reaction chamber, (ii) a single wax solution which is optionally contained within the second reaction chamber, (iii) a source of radiant energy which is transmitted into the second reaction chamber to heat the wax solution, and (iv) a vacuum pump connected to the second reaction chamber;\n
the method comprising:\n
(a) initially impregnating a tissue specimen in the first module with the single non-aqueous admixture comprised of (i) at least one ketone, (ii) at least one alcohol, and (iii) mineral oil while being agitated and heated then\n
(b) substantially impregnating the tissue specimen in the second module with the single wax solution while being under vacuum and heated."],"number":69,"annotation":false,"claim":true,"title":false}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}