Methods For Purifying Fc-containing Proteins

Abstract

The present invention provides a process for dissociating Fc-containing molecules from complexes of Protein A/Fc-containing molecules or mixtures containing Fc-containing molecules and Protein A. The association, e.g., by hydrophobic interactions, between the Fc-containing molecules and Protein A can be reduced or inhibited by raising the pH of dissociation. The pH of dissociation can be raised by addition of agents capable of inhibiting hydrophobic interactions, including buffers containing arginine and/or ethylene glycol, to the mixture, either prior to adding the mixture to the column chromatography substrate, after adding the mixture to the column chromatography substrate, or both prior to and after adding the mixture to the column chromatography substrate. Separation of Fc-containing molecules from Protein A can be performed on a number of different column chromatographic substrates, including column chromatographic substrates contained in Q columns (Q column chromatography substrate), HIC columns (hydrophobic interaction column chromatography substrate), and IMAC columns (metal chelate column chromatography substrate).


Claims
Download PDF
Document Preview
Document History
  • Publication: May 29, 2007
  • Application: Jun 5, 2002
    US US 16385302 A
  • Priority: Jun 5, 2002
    US US 16385302 A
  • Priority: Jun 5, 2001
    US US 29640201 P

Download Citation


Sign in to the Lens

Feedback