{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"111-980-028-744-660","frontPageModel":{"patentViewModel":{"ref":{"entityRefId":"111-980-028-744-660","entityRefType":"PATENT"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":8905,"type":"PATENT","title":"SFIT Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":4158,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8220,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search applicants and owners = \"Swiss Federal Institute of Technology\",\"Swis* Fed* Inst* Tech*\" , \"ETH Zurich\", \"ETH Transfer\", \"ETH\".
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Total patents: 2709
Search applicants and owners = \"Swiss Federal Institute of Technology\",\"Swis* Fed* Inst* Tech*\" , \"ETH Zurich\", \"ETH Transfer\", \"ETH\".
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Total patents: 2709
1. Introducing a set of genetic elements into said mammalian cell, the set of genetic elements forming a differentiation-control network which allows for an inducible expression pattern of the transcription factors Pdx1, Ngn3 and MafA or functional fragments thereof;
2. Exposing the mammalian cell to conditions which allow for the differentially timed expression of said transcription factors, thereby differentiating the mammalian cell into a pancreatic beta-like cell."],"number":12,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 12, wherein the expression pattern of said transcription factors comprises three sequential phases,
(iv)A first phase in which expression of Pdx1 is induced, Ngn3 expression is repressed and MafA expression is repressed;
(v) A second phase in which Pdx1 expression is repressed, Ngn3 expression is induced and MafA expression is repressed;
(vi)A third phase in which Ngn3 expression is repressed and expression MafA and Pdx1 is induced."],"number":13,"annotation":false,"claim":true,"title":false},{"lines":["The method of any one of claims 12 to 13 wherein said mammalian cell is a stem cell, an induced pluripotent stem cell, a somatic cell, an endoderm cell, a pancreatic progenitor cell or an endocrine progenitor cell."],"number":14,"annotation":false,"claim":true,"title":false},{"lines":["The method of any one of claims 12 to 14, wherein the mammalian cell, the pancreatic beta-like cell or any intermediate thereof comprises the expression system of any one of claims 1 to 9."],"number":15,"annotation":false,"claim":true,"title":false},{"lines":["The method of any one of claims 12 to 15, wherein the expression pattern is controlled by adding varying concentrations of vanillic acid to the cells."],"number":16,"annotation":false,"claim":true,"title":false},{"lines":["A method of differentiating a cell, comprising the steps of
a. Introducing the expression system of any one of claims 1 to 9 into said cell;
b. Adding varying doses of one or more ligands to said cell, thereby triggering the sequential expression of two or more genes of interest and altering the phenotype of said cell."],"number":17,"annotation":false,"claim":true,"title":false}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}