{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"111-296-321-771-659","frontPageModel":{"patentViewModel":{"ref":{"entityRefId":"111-296-321-771-659","entityRefType":"PATENT"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":11766,"type":"PATENT","title":"Florida State University Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":2590,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8404,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately: florida state uni*. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 2443
Search Applicants and Owners separately: florida state uni*. Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 2443
linking via a first antibody a magnetic microparticle to the target antigen in the physiological fluid;\n
utilizing a magnetic field to separate the magnetic microparticle and linked target antigen from at least a portion of other components in the physiological fluid, thereby forming a test sample;\n
linking via a second antibody a glucose molecule to the target antigen; and\n
detecting the glucose in the test sample to determine the concentration of target antigen in the physiological fluid."],"number":1,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein the target antigen comprises a protein or marker resulting from cardiac tissue injury."],"number":2,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein the target antigen comprises myoglobin."],"number":3,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein the target antigen is selected from the group consisting of creatine kinase, fatty acid-binding protein, troponins, and glycogen phosphorylase."],"number":4,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein the physiological fluid comprises blood, serum, or urine."],"number":5,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein the glucose detection comprises measuring the electrical resistance, color, or pH of the test sample."],"number":6,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein the glucose detection comprise measuring light transmittance through the test sample."],"number":7,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein the glucose detection comprises oxidation of the glucose to gluconic acid at a platinum electrode."],"number":8,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein the second antibody is linked to a streptavidin."],"number":9,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 9, wherein the streptavidin is linked to a lectin which is linked to the glucose molecule."],"number":10,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein the second antibody is a biotinylated anti-myoglobin antibody and the anti-myoglobin antibody is linked to streptavidin which is linked to a biotinylated Con-A lectin which is linked to the glucose molecule."],"number":11,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein the first and second antibodies comprise complementary clones of monoclonal anti-human cardiac myoglobin antibody."],"number":12,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 12, wherein the second antibody comprises a monoclonal mouse anti-human cardiac myoglobin Mouse isotope IgG:908."],"number":13,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 12, wherein the first antibody comprises a monoclonal mouse anti-human cardiac myoglobin Mouse isotope IgG:4E2."],"number":14,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein the magnetic microparticles comprise microspheres coated with a polymer or protein."],"number":15,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 15, wherein the coating comprises albumin, avidin, or a combination thereof."],"number":16,"annotation":false,"claim":true,"title":false},{"lines":["A method for detecting an Acute Myocardial Infarction-indicating antigen in a physiological sample comprising:\n
combining the physiological sample with magnetic microparticles linked to a first biotinylated antibody, said first antibody binding to a first site on the AMI-indicating antigen present in the sample;\n
applying a magnetic field to the physiological sample to separate the linked Acute Myocardial Infarction-indicating antigen from the blood sample to form a test sample comprising the magnetic particle-linked AMI-indicating antigen;\n
combining the test sample with a second biotinylated antibody, said second antibody binding to a second site on the Acute Myocardial Infarction-indicating antigen, wherein said second antibody is linked to a glucose molecule; and\n
detecting the glucose in the test sample to determine the concentration of AMI-indicating antigen in the blood sample."],"number":17,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 17, wherein the physiological sample is blood, serum, or urine."],"number":18,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 17, wherein the Acute Myocardial Infarction-indicating antigen comprises myoglobin."],"number":19,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 17, wherein the glucose detection comprises measuring the electrical resistance, color, or pH of the test sample."],"number":20,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 17, wherein the glucose detection comprises oxidation of the glucose to gluconic acid at a platinum electrode."],"number":21,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 17, wherein the second antibody is a biotinylated anti-myoglobin antibody and the anti-myoglobin antibody is linked to streptavidin which is linked to a biotinylated Con-A lectin which is linked to the glucose molecule."],"number":22,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 17, wherein the first and second antibodies comprise complementary clones of monoclonal anti-human cardiac myoglobin antibody."],"number":23,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 17, wherein the second antibody comprises a monoclonal mouse anti-human cardiac myoglobin Mouse isotope IgG:908."],"number":24,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 17, wherein the first antibody comprises a monoclonal mouse anti-human cardiac myoglobin Mouse isotope IgG:4E2."],"number":25,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 17, wherein the microparticles are microspheres which comprise a core of a superparamagnetic material coated with a protein or polymer."],"number":26,"annotation":false,"claim":true,"title":false},{"lines":["A method for detecting a target antigen in a physiological fluid comprising:\n
linking via a first antibody a magnetic microparticle to the target antigen in the physiological fluid;\n
utilizing a magnetic field to separate the magnetic micro particle with the target antigen linked thereto from at least a portion of other components in the physiological fluid, thereby forming a test sample;\n
linking via a second antibody a glucose molecule to the target antigen; and\n
detecting, using a colorimetric method, the linked glucose molecule in the test sample to determine the concentration of target antigen in the physiological fluid."],"number":27,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 27, wherein the target antigen is myoglobin."],"number":28,"annotation":false,"claim":true,"title":false}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}