{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"107-113-181-584-325","frontPageModel":{"patentViewModel":{"ref":{"entityRefId":"107-113-181-584-325","entityRefType":"PATENT"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":10784,"type":"PATENT","title":"Purdue University - Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":7369,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8254,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners separately: \"Purdue Uni*\";
Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 467
Search Applicants and Owners separately: \"Purdue Uni*\";
Select more for logical variants. Add to collection. Select all patents in the collection and expand by simple families. Add to collection. Total patents: 467
applying a first magnetic field at or above 0.1 Tesla to a layer of a first collagen solution defining a horizontal plane, within a temperature at or between 2° C. and 45° C., to align a plurality of collagen fibrils formed within the first collagen solution before fibrillogenesis is complete and generate an aligned collagen layer having a planar fibril alignment and a thickness;\n
selecting or altering the temperature as a tunable parameter to manipulate the rate of fibrillogenesis of the collagen fibrils to achieve a targeted degree of alignment of the collagen fibrils across the entirety of the thickness of the aligned collagen layer;\n
combining the aligned collagen layer with a second layer of collagen, the second layer of collagen positioned adjacent to the aligned collagen layer to form a construct and comprising a quantity of collagen solution selected from a group consisting of a first quantity of a second collagen solution and a second quantity of the first collagen solution; and\n
adding to the construct by combining the aligned collagen layer and the second layer of collagen with a third layer of collagen, the third layer of collagen positioned adjacent to one or both of the aligned collagen layer and the second layer of collagen and comprising a quantity of collagen solution selected from a group consisting of the first quantity of the second collagen solution, a second quantity of the second collagen solution, the second quantity of the first collagen solution, and a third quantity of the first collagen solution;\n
wherein the temperature is tuned such that the step of applying takes longer than 30 minutes to achieve at least 95% fibrillogenesis of the collagen fibrils and at least one of the second and third layers of collagen of the construct consists of collagen fibrils that are randomly aligned."],"number":1,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein the step of applying is performed at a temperature of less than 20° C."],"number":2,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein:\n
the step of applying is performed to align the collagen fibrils within the aligned collagen layer at an orientation angle range of at or between plus or minus 10°;\n
the step of selecting or altering the temperature as a tunable parameter to manipulate the rate of fibrillogenesis comprises the steps of decreasing the rate of fibrillogenesis and delaying polymerization initiation."],"number":3,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein the first magnetic field is applied at a direction selected from the group consisting of a direction of the horizontal plane and a direction perpendicular to the horizontal plane; and\n
the targeted degree of alignment of the collagen fibrils comprises a parallelism index of between 0.6 and 0.8 throughout the entirety of the thickness of the collagen layer."],"number":4,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein the step of combining further comprises the step of applying a magnetic field selected from the group consisting of the first magnetic field and a second magnetic field to the second layer of collagen to align collagen within the second layer of collagen relative to the selected magnetic field."],"number":5,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 5, wherein the first magnetic field is at or about 90° relative to the second magnetic field."],"number":6,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein the step of adding to the construct further comprises the step of applying a magnetic field selected from the group consisting of the first magnetic field and the second magnetic field to the third layer of collagen to align collagen within the third layer of collagen relative to the selected magnetic field."],"number":7,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, further comprising the step of:\n
adding one or more additional layers of a collagen solution to the construct."],"number":8,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein:\n
the first collagen solution comprises collagen and at least one substance selected from the group consisting of one or more minerals, one or more proteoglycans, hydroxyapatite, calcium, phosphorous, a combination of calcium and phosphorous, aggrecan, chondroitin sulfate, dermatan sulfate, genepin, hyaluronic acid, 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide, one or more cells, one or more primary mammalian cells, and one or more progenitor mammalian cells, and wherein the second collagen solution comprises collagen and is at least substantially free of the selected at least one substance; and\n
the amount of the at least one substance in the first collagen solution is selected to achieve a desired effect on the fibril alignment of the aligned collagen layer."],"number":9,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, further comprising the step of:\n
administering at least the aligned collagen layer to a patient to treat a condition experienced by the patient, the condition selected from the group consisting of an arthritic condition, a cartilage-based condition, a bone-based condition, a ligament condition, and a tendon condition."],"number":10,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein the second layer of collagen consists of collagen fibrils that are randomly aligned and the second layer of collagen is positioned between the aligned layer of collagen and the third layer of collagen."],"number":11,"annotation":false,"claim":true,"title":false},{"lines":["A method of treating a patient, comprising the step of:\n
administering a construct to a patient to treat a patient condition, the construct formed by:\n"],"number":12,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 12, wherein the step of combining further comprises the step of applying a magnetic field selected from the group consisting of the first magnetic field and a second magnetic field to the second layer of collagen to align collagen within the second layer of collagen relative to the selected magnetic field."],"number":13,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 12, wherein the step of adding one or more additional layers further comprises positioning a randomly aligned layer of collagen adjacent to the second layer of collagen."],"number":14,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 14, wherein the second layer of collagen is positioned between the randomly aligned layer of collagen and the first layer of collagen."],"number":15,"annotation":false,"claim":true,"title":false},{"lines":["A method of generating an aligned collagen layer, comprising:\nforming a first layer of aligned collagen by applying a first direction magnetic field to a first quantity of a first collagen solution at a temperature of at or between 10° C. and 25° C. to align a plurality of collagen fibrils formed within the first collagen solution in a planar alignment relative to the first direction magnetic field before fibrillogenesis is complete;\nselecting or altering the temperature as a tunable parameter to manipulate the rate of fibrillogenesis of the collagen fibrils to achieve a targeted degree of alignment of the collagen fibrils across the entirety of the thickness of the collagen layer, the temperature tuned such that the step of applying takes longer than 30 minutes to achieve at least 95% fibrillogenesis of the collagen fibrils;\ncombining the first layer of collagen with a second layer of collagen to form a construct, the second layer of collagen positioned adjacent to the first layer of collagen and comprising a quantity of a collagen solution selected from the group consisting of a first quantity of a second collagen solution and a second quantity of the first collagen solution; and\nadding one or more additional layers of a collagen solution to the construct, wherein at least one of the additional layers consists of collagen fibrils that are randomly aligned.\n
applying a first magnetic field at or above 0.1 Tesla to a layer of a first collagen solution defining a horizontal plane, within a temperature at or between 2° C. and 45° C., to align a plurality of collagen fibrils formed within the first collagen solution before fibrillogenesis is complete and generate an aligned collagen layer having a planar fibril alignment and a thickness;\n
selecting or altering the temperature as a tunable parameter to manipulate the rate of fibrillogenesis of the collagen fibrils to achieve a targeted degree of alignment of the collagen fibrils across the entirety of the thickness of the aligned collagen layer;\n
crosslinking the aligned collagen layer, resulting in an individual aligned crosslinked collagen layer;\n
combining the aligned collagen layer with a randomly aligned layer of collagen, the randomly aligned layer of collagen consisting of collagen fibrils that are randomly aligned;\n
wherein the temperature is tuned such that the step of applying takes longer than 30 minutes to achieve at least 95% fibrillogenesis of the collagen fibrils."],"number":16,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 16, further comprising the step of:\n
combining the aligned collagen layer with a second layer of collagen, the second layer of collagen comprising a quantity of a collagen solution selected from the group consisting of a first quantity of a second collagen solution and a second quantity of the first collagen solution;\n
wherein the second layer of collagen is positioned adjacent to the aligned collagen layer, forming a construct."],"number":17,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 17, wherein the step of combining further comprises the step of applying a magnetic field selected from the group consisting of the first magnetic field and a second magnetic field to the second layer of collagen to align collagen within the second layer of collagen relative to the selected magnetic field."],"number":18,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 17, further comprising the step of:\n
further combining the aligned collagen layer and the second layer of collagen with a third layer of collagen, the third layer of collagen comprising a quantity of a collagen solution selected from the group consisting of the first quantity of the second collagen solution, a second quantity of the second collagen solution, the second quantity of the first collagen solution, and a third quantity of the first collagen solution;\n
wherein the third layer of collagen is positioned adjacent to one or both of the aligned collagen layer and the second layer of collagen, adding to the construct."],"number":19,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 17, wherein the randomly aligned layer of collagen is positioned between the aligned collagen layer and the second layer of collagen."],"number":20,"annotation":false,"claim":true,"title":false}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}