{"search_session":{},"preferences":{"l":"en","queryLanguage":"en"},"patentId":"107-070-211-908-646","frontPageModel":{"patentViewModel":{"ref":{"entityRefId":"107-070-211-908-646","entityRefType":"PATENT"},"entityMetadata":{"linkedIds":{"empty":true},"tags":[],"collections":[{"id":8892,"type":"PATENT","title":"University of Wisconsin Patent Portfolio","description":"","access":"OPEN_ACCESS","displayAvatar":true,"attested":false,"itemCount":15082,"tags":[],"user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"notes":[{"id":8214,"type":"COLLECTION","user":{"id":91044780,"username":"Cambialens","firstName":"","lastName":"","created":"2015-05-04T00:55:26.000Z","displayName":"Cambialens","preferences":"{\"usage\":\"public\",\"beta\":false}","accountType":"PERSONAL","isOauthOnly":false},"text":"
Search Applicants and Owners = \"Wisconsin University\" , \"University of Wisconsin\", \"Wisconsin Univ\", \" Univ Wisconsin\" , \"Wisco* Alum* Res* Found*\".
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Search Applicants and Owners = \"Wisconsin University\" , \"University of Wisconsin\", \"Wisconsin Univ\", \" Univ Wisconsin\" , \"Wisco* Alum* Res* Found*\".
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(a) introducing into a 293T cell, expression vectors which direct expression in said cells of genomic vRNA segments or antigenomic cRNAs of said genomic vRNA segments, a nucleoprotein, and an RNA-dependent RNA polymerase, so that ribonucleoprotein complexes are formed and viral particles of said virus are assembled in the absence of helper virus; and\n
(b) culturing the resulting cells of step (a) so that viral particles of said virus are packaged and rescued."],"number":1,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 1, wherein said expression of genomic vRNA segments or antigenomic cRNAs of said genomic vRNA segments in the expression vectors is directed by an RNA polymerase I promoter."],"number":2,"annotation":false,"claim":true,"title":false},{"lines":["A method for generating, in cultured cells, infectious influenza viral particles, said method comprising:\n
(a) introducing into cultured cells, expression vectors that direct expression of genomic vRNA segments or antigenomic cRNAs of said genomic vRNA segments, a nucleoprotein, and an RNA-dependent RNA polymerase, so that ribonucleoprotein complexes containing the genomic vRNA segments of said virus are formed and said viral particles are assembled within said cells in the absence of helper virus; and\n
(b) culturing the resulting cells of step (a) so that said viral particles are produced."],"number":3,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 3, wherein the cultured cells comprise expression vectors that direct expression of said nucleoprotein and subunits of said RNA-dependent RNA polymerase."],"number":4,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 3 or 4, wherein said virus is an influenza virus of type A, B or C."],"number":5,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 3, further comprising the step of isolating infectious viral particles."],"number":6,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 3, further comprising after step (b), the step of attenuating said viral particles."],"number":7,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 3, wherein said expression vectors are introduced by transfection into said cells in the presence of a liposomal transfection reagent."],"number":8,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 3, wherein said expression vectors are plasmids."],"number":9,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 3, wherein each genomic vRNA segment, or antigenomic cRNA of said genomic vRNA segment, is encoded on a separate expression vector."],"number":10,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 3, wherein expression of each genomic vRNA segment or antigenomic cRNA is under control of a RNA polymerase I promoter."],"number":11,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 11, wherein said RNA polymerase I promoter is a human RNA polymerase I promoter."],"number":12,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 3, wherein a coding sequence for each genomic vRNA segment or corresponding antigenomic cRNA in said expression vectors is upstream of a transcription terminator."],"number":13,"annotation":false,"claim":true,"title":false},{"lines":["A method for generating, in cultured cells, infectious influenza viral particles, said method comprising:\n
(i) introducing into cells, expression vectors directing expression in said cells of:\n
(a) genomic vRNAs of said virus in the absence of a helper virus; and\n
(b) a nucleoprotein and RNA-dependent RNA polymerase, wherein ribonucleoprotein complexes containing said genomic vRNAs are formed and said viral particles are assembled, wherein said genomic vRNAs are expressed in said cells under the control of a RNA polymerase I promoter; and\n
(ii) culturing the resulting cells of step (i) so that said viral particles are produced."],"number":14,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 14, further comprising the step of infecting cells with the viral particles produced from step (ii)."],"number":15,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 14, further comprising the step of isolating said infectious viral particles."],"number":16,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 14, further comprising after performing step (ii), the step of attenuating said viral particles."],"number":17,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 17, further comprising incorporating the resulting attenuated viral particles into a vaccine composition."],"number":18,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 17, wherein said virus has at least one vRNA segment that directs expression of a sequence heterologous to said virus in target cells infected by said virus, and said method further comprises the step of incorporating said viral particles, optionally after attenuation, into a vaccine composition."],"number":19,"annotation":false,"claim":true,"title":false},{"lines":["A method for rescuing a chimeric recombinant influenza virus, wherein said chimeric recombinant influenza virus expresses heterologous nucleic acid sequences, comprising:\n
(a) introducing into a 293T cell, expression vectors that direct expression in said cells of genomic vRNA segments comprising heterologous nucleic acid sequences, or antigenomic cRNAs of said genomic vRNA segments further comprising heterologous nucleic acid sequences, a nucleoprotein, and an RNA-dependent RNA polymerase, so that ribonucleoprotein complexes are formed and viral particles of said virus are assembled in the absence of helper virus; and\n
(b) culturing the resulting cells of step (a), wherein viral particles are packaged and rescued."],"number":20,"annotation":false,"claim":true,"title":false},{"lines":["A method for generating, in cultured cells, infectious viral particles of a chimeric influenza virus, wherein said chimeric influenza virus expresses heterologous nucleic acid sequences, comprising:\n
(a) introducing into cultured cells, expression vectors that direct expression of genomic vRNA segments comprising heterologous nucleic acid sequences or antigenomic cRNAs of said genomic vRNA segments comprising heterologous nucleic acid sequences, a nucleoprotein, and an RNA-dependent RNA polymerase so that ribonucleoprotein complexes containing the genomic vRNA segments of said chimeric influenza virus are formed and said viral particles are assembled within said cells in the absence of helper virus; and\n
(b) culturing the resulting cells of step (a), wherein said viral particles are produced."],"number":21,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 21, wherein said virus is an influenza virus of type A, B or C."],"number":22,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 21, further comprising the step of infecting cells with the viral particles produced from step (b)."],"number":23,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 21, further comprising the step of isolating infectious viral particles."],"number":24,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 21, further comprising after step (b), the step of attenuating said viral particles."],"number":25,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 21, wherein said expression vectors are introduced by transfection into said cells in the presence of a liposomal transfection reagent."],"number":26,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 21, wherein said expression vectors are plasmids."],"number":27,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 21, wherein each genomic vRNA segment, or each antigenomic cRNA of said genomic vRNA segment, is encoded on a separate expression vector."],"number":28,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 21, wherein expression of each vRNA segment or cRNA is under control of a RNA polymerase I promoter."],"number":29,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 21, wherein the coding sequence for each vRNA segment or cRNA in said expression vectors is upstream of a transcription terminator."],"number":30,"annotation":false,"claim":true,"title":false},{"lines":["A method for generating, in cultured cells, infectious chimeric influenza viral particles, wherein chimeric influenza virus of said particles expresses heterologous nucleic acid sequences, said method comprising:\n
(i) introducing into cells, expression vectors directing expression in said cells of:\n
(a) genomic vRNAs of said chimeric virus in the absence of a helper virus; and\n
(b) a nucleoprotein and RNA-dependent RNA polymerase so that ribonucleoprotein complexes containing said genomic vRNAs are formed and said viral particles are assembled, wherein said genomic vRNAs are expressed in said cells under the control of a RNA polymerase I promoter; and\n
(ii) culturing the resulting cells of step (i), wherein said viral particles are produced."],"number":31,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 31, further comprising the step of infecting cells with the viral particles produced from step (ii)."],"number":32,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 31, further comprising the step of isolating infectious viral particles."],"number":33,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 31, further comprising after step (b), the step of attenuating said viral particles."],"number":34,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 34, further comprising the step of incorporating the resulting attenuated viral particles into a vaccine composition."],"number":35,"annotation":false,"claim":true,"title":false},{"lines":["A method for rescuing influenza viral particles comprising culturing a 293T cell comprising expression vectors that direct expression of:\n
(a) genomic vRNA segments or antigenomic cRNAs of said genomic vRNA segments,\n
(b) a nucleoprotein, and\n
(c) an RNA-dependent RNA polymerase,\n
wherein ribonucleoprotein complexes containing genomic vRNA segments are formed and viral particles are assembled in said 293T cell in the absence of helper virus, and wherein viral particles are packaged and rescued from said 293T cell."],"number":36,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 36, wherein said influenza virus is influenza virus type A, B or C."],"number":37,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 37, wherein said DNA-dependent RNA polymerase promoter is an RNA polymerase I promoter."],"number":38,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 36, wherein said expression of genomic vRNA segments or antigenomic cRNAs of said genomic vRNA segments in the expression vectors is directed by a DNA-dependent RNA polymerase promoter."],"number":39,"annotation":false,"claim":true,"title":false},{"lines":["A method for generating, in cultured cells, infectious viral particles of an influenza virus, said method comprising culturing cells comprising expression vectors which direct expression of:\n
(a) genomic vRNA segments or antigenomic cRNAs of said genomic vRNA segments,\n
(b) a nucleoprotein, and\n
(c) an RNA-dependent RNA polymerase,\n
wherein ribonucleoprotein complexes containing the genomic vRNA segments are formed and said viral particles are assembled within said cells in the absence of helper virus."],"number":40,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 40, wherein said influenza virus is influenza virus type A, B or C."],"number":41,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 40, further comprising the step of isolating infectious viral particles."],"number":42,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 40, further comprising the step of attenuating said viral particles."],"number":43,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 40, wherein said cells are obtained by introducing by transfecting said expression vectors in the presence of liposomal transfection reagent."],"number":44,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 40, wherein said expression vectors are plasmids."],"number":45,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 40, wherein each genomic vRNA segment, or each antigenomic cRNA of said genomic vRNA segment, is encoded on a separate expression vector."],"number":46,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 40, wherein expression of each genomic vRNA segment or antigenomic cRNA is under control of a DNA-dependent RNA polymerase promoter."],"number":47,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 47, wherein said DNA-dependent RNA polymerase promoter is an RNA polymerase I promoter."],"number":48,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 48, wherein said RNA polymerase I promoter is human RNA polymerase I promoter."],"number":49,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 40, wherein said expression vectors further comprise a coding sequence for each genomic vRNA segment or antigenomic cRNA upstream of a transcription terminator."],"number":50,"annotation":false,"claim":true,"title":false},{"lines":["A method for generating in cultured cells, infectious influenza viral particles, said method comprising culturing cells comprising expression vectors directing expression of:\n
(a) genomic vRNA segments of an influenza virus,\n
(b) a nucleoprotein, and\n
(c) RNA-dependent RNA polymerase,\nwherein ribonucleoprotein complexes containing the genomic vRNA segments are formed and said viral particles are assembled in said cells in the absence of a helper virus, wherein expression of said genomic vRNAs in said cells is under control of a RNA polymerase I promoter."],"number":51,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 51, wherein said influenza virus is influenza virus type A, B or C."],"number":52,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 51, further comprising the step of isolating said infectious viral particles."],"number":53,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 51, further comprising the step of attenuating said viral particles."],"number":54,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 54, further comprising the step of incorporating the resulting attenuated viral particles into a vaccine composition."],"number":55,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 51, wherein said virus has at least one vRNA segment directing expression of a sequence heterologous to said virus in target cells infected by said virus, and said method further comprising the step of incorporating said viral particles, optionally after attenuation, into a vaccine composition."],"number":56,"annotation":false,"claim":true,"title":false},{"lines":["A method for rescuing a chimeric recombinant influenza expressing heterologous nucleic acid sequences, comprising culturing a 293T cell comprising expression vectors directing expression of:\n
(a) genomic vRNA segments comprising heterologous nucleic acid sequences or antigenomic cRNAs of said genomic vRNA segments comprising heterologous nucleic acid sequences,\n
(b) a nucleoprotein, and\n
(c) an RNA-dependent RNA polymerase,\n
wherein ribonucleoprotein complexes containing the genomic vRNA segments are formed and viral particles are assembled in the absence of helper virus; and wherein viral particles are packaged and rescued."],"number":57,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 57, wherein said influenza virus is influenza virus type A, B or C."],"number":58,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 57, further comprising the step of isolating infectious viral particles."],"number":59,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 57, further comprising the step of attenuating said viral particles."],"number":60,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 60, further comprising the step of incorporating the resulting attenuated viral particles into a vaccine composition."],"number":61,"annotation":false,"claim":true,"title":false},{"lines":["A method for generating, in cultured cells, infectious viral particles of a chimeric influenza virus, wherein said chimeric influenza virus expresses heterologous nucleic acid sequences, comprising culturing cells comprising expression vectors directing the expression of:\n
(a) genomic vRNA segments comprising heterologous nucleic acid sequences or antigenomic cRNAs comprising heterologous nucleic acid sequences of said genomic vRNA segments, and\n
(b) a nucleoprotein, and\n
(c) an RNA-dependent RNA polymerase,\n
wherein ribonucleoprotein complexes containing the genomic vRNA segments are formed and said viral particles are assembled within said cells in the absence of helper virus."],"number":62,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 62, wherein said virus is an influenza virus of type A, B or C."],"number":63,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 62, further comprising the step of isolating infectious viral particles."],"number":64,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 62, further comprising the step of attenuating said viral particles."],"number":65,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 62, wherein said cells are obtained by introducing by transfecting said expression vectors in the presence of a liposomal transfection reagent."],"number":66,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 62, wherein said expression vectors are plasmids."],"number":67,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 62, wherein each genomic vRNA segment, or each antigenomic cRNA of said genomic vRNA segment, is encoded on a separate expression vector."],"number":68,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 62, wherein the expression of each vRNA segment or cRNA is under control of a promoter sequence that is a DNA-dependent RNA polymerase promoter."],"number":69,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 62, wherein said mammalian DNA-dependent RNA polymerase promoter is a RNA polymerase I promoter."],"number":70,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 62, wherein a coding sequence for each vRNA segment or cRNA in said expression vectors is upstream of a transcription terminator."],"number":71,"annotation":false,"claim":true,"title":false},{"lines":["A method for generating in cultured cells, infectious viral particles of a chimeric influenza virus expressing heterologous nucleic acid sequences, said method comprising culturing cells comprising expression vectors directing expression of:\n
(a) genomic vRNA segments of said chimeric influenza virus;\n
(b) a nucleoprotein; and\n
(c) RNA-dependent RNA polymerase,\nwherein ribonucleoprotein complexes containing the genomic vRNA segments are formed and said viral particles are assembled in said cells in the absence of helper virus, wherein expression of said genomic vRNAs in said cells is under the control of a RNA polymerase I promoter."],"number":72,"annotation":false,"claim":true,"title":false},{"lines":["A method for rescuing influenza viral particles comprising culturing cells comprising expression vectors directing expression of genomic vRNA segments of an influenza virus, and expression vectors directing expression of nucleoprotein and RNA-dependent RNA polymerase,\n
wherein the genomic vRNA segments are PB2 (Protein basic 2), PB1 (Protein basic 1), PA (Protein acidic), HA (Haemagglutinin), NP (Nucleoprotein), NA (Neuraminidase), M (Matrix protein), and NS (Non-structural protein),\n
wherein ribonucleoprotein complexes containing the genomic vRNA segments are formed and viral particles are assembled in said cells in the absence of helper virus, and such that viral particles are packaged and rescued from said cells,\n
wherein transcription of each of the genomic vRNA segments is controlled by an RNA polymerase I promoter, and\n
wherein expression of nucleoprotein and RNA-dependent RNA polymerase is controlled by an RNA polymerase II promoter."],"number":73,"annotation":false,"claim":true,"title":false},{"lines":["The method of claim 73, wherein said influenza virus is influenza virus type A."],"number":74,"annotation":false,"claim":true,"title":false}]}},"filters":{"npl":[],"notNpl":[],"applicant":[],"notApplicant":[],"inventor":[],"notInventor":[],"owner":[],"notOwner":[],"tags":[],"dates":[],"types":[],"notTypes":[],"j":[],"notJ":[],"fj":[],"notFj":[],"classIpcr":[],"notClassIpcr":[],"classNat":[],"notClassNat":[],"classCpc":[],"notClassCpc":[],"so":[],"notSo":[],"sat":[]},"sequenceFilters":{"s":"SEQIDNO","d":"ASCENDING","p":0,"n":10,"sp":[],"si":[],"len":[],"t":[],"loc":[]}}