Microbial Trypsin Mutants Having Chymotrypsin Activity And Nucleic Acids Encoding Same

  • Published: Feb 5, 2009
  • Earliest Priority: Sep 24 2002
  • Family: 19
  • Cited Works: 0
  • Cited by: 0
  • Cites: 0
  • Sequences: 24
  • Additional Info: Full text Sequence
Abstract

The present invention relates to microbial trypsin variants having chymotrypsin-like activity, comprising: (a) a one or more substitutions corresponding to positions 144, S193A, 198, 201, 218, 223, 227, 228, 229, 230, and 231 of amino acids 25 to 248 of SEQ ID NO: 2, (b) one or more deletions corresponding to positions 192, 197, and 226 of amino acids 25 to 248 of SEQ ID NO: 2; and (c) an insertion between positions corresponding to positions 224 and 225 of amino acids 25 to 248 of SEQ ID NO: 2. The present invention further relates to nucleotide sequences encoding microbial trypsin variants having chymotrypsin-like activity; nucleic acid constructs, expression vectors, and recombinant host cells comprising such nucleotide sequences; and methods of producing microbial trypsin variants having chymotrypsin-like activity or a precursor thereof.


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Document History
  • Publication: Feb 5, 2009
  • Application: Jan 30, 2008
    US US 2277108 A
  • Priority: Jan 30, 2008
    US US 2277108 A
  • Priority: Sep 24, 2003
    US US 66975703 A
  • Priority: Sep 24, 2002
    US US 41305702 P

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