Abstract

A method for simply and conveniently detecting acetyltransferase and deacetylase activities of proteins by executing an acetylation reaction of a peptide substrate with an acetyltransferase, or a deacetylation reaction of an acetylated peptide substrate with a deacetylase, and after the completion of these reactions, detecting the acetyl group bound to the peptide substrate by using an anti-acetylated peptide antibody. This system for detecting acetyltransferase and deacetylase activities using the anti-acetylated peptide antibody enables screening inhibitors or enhancers of acetyltransferase and deacetylase. A system for screening deacetylase inhibitors or acetyltransferase enhancers using cultured cells is also provided.


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Document History
  • Publication: Apr 26, 2005
  • Application: Jul 18, 2000
    US US 61842400 A
  • Priority: Jul 18, 2000
    US US 61842400 A
  • Priority: Jan 20, 1999
    JP 9900191 W
  • Priority: Jan 20, 1998
    JP 917198 A

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