Nematode Resistant Crops

  *US10231383B2*
  US010231383B2                                 
(12)United States Patent(10)Patent No.: US 10,231,383 B2
 Mitchum et al. (45) Date of Patent:Mar.  19, 2019

(54)Nematode resistant crops 
    
(75)Inventors: Melissa G. Mitchum,  Columbia, MO (US); 
  Amy Replogle,  Columbia, MO (US); 
  Jianying Wang,  Columbia, MO (US); 
  Xiaohong Wang,  Washington, DC (US); 
  Shiyan Chen,  Ithaca, NY (US); 
  Ping Lang,  Ithaca, NY (US); 
  Eric L. Davis,  Raleigh, NC (US); 
  Thomas J. Baum,  Ames, IA (US); 
  Richard S. Hussey,  Athens, GA (US) 
(73)Assignees:The Curators of the University of Missouri,  Columbia, MO (US), Type: US Company;
Cornell University,  Ithaca, NY (US), Type: US Company;
North Carolina State University,  Raleigh, NC (US), Type: US Company;
Iowa State University Research Foundation, Inc.,  Ames, IA (US), Type: US Company;
University of Georgia Research Foundation, Inc.,  Athens, GA (US), Type: US Company;
The United States of America, as represented by the Secretary of Agriculture,  Washington, DC (US), Type: US Gov't
 
(*)Notice: Subject to any disclaimer, the term of this patent is extended or adjusted under 35 U.S.C. 154(b) by 143 days. 
(21)Appl. No.: 13/814,591 
(22)PCT Filed:Jul.  13, 2011 
(86)PCT No.: PCT/US2011/043882 
 § 371 (c)(1), (2), (4) Date: Aug.  22, 2013  
(87)PCT Pub. No.:WO20/12/018489 
 PCT Pub. Date:Feb.  9, 2012 
(65)Prior Publication Data 
 US 2013/0326736 A1 Dec.  5, 2013 
 Related U.S. Patent Documents 
(60)Provisional application No. 61/371,619, filed on Aug.  6, 2010.
 
Jan.  1, 2013 A 01 D 91 00 F I Mar.  19, 2019 US B H C Jan.  1, 2013 C 07 K 14 415 L I Mar.  19, 2019 US B H C Jan.  1, 2013 C 12 N 15 8285 L I Mar.  19, 2019 US B H C Jan.  1, 2018 Y 02 A 40 164 L A Mar.  19, 2019 US B H C
(51)Int. Cl. A01D 091/00 (20060101); C07K 014/415 (20060101); C12N 015/82 (20060101)
(58)Field of Search  None

 
(56)References Cited
 
 U.S. PATENT DOCUMENTS
 8,569,578  B1*10/2013    Wang 435/320.1
 2002//0092041  A1  7/2002    Ronald et al.     
 2003//0005491  A1  1/2003    Hauge et al.     
 2004//0067506  A1*4/2004    Scheres et al. 435/6
 2006//0080749  A1  4/2006    Hussey et al.     
 2006//0162019  A1  7/2006    Palva et al.     
 2009//0012029  A1  1/2009    Hussey et al.     
 2009//0019601  A1  1/2009    Kovalic     
 2009//0077687  A1  3/2009    Hussey et al.     
 2009//0241218  A1*9/2009    Frankard et al. 800/278
 2010//0186129  A1  7/2010    Hussey et al.     
 2010//0192257  A1  7/2010    Jones et al.     
 2010//0281572  A1  11/2010    Hussey et al.     
 2012//0192315  A1  7/2012    Lightfoot     
 2013//0326736  A1  12/2013    Mitchum et al.     
 2013//0333061  A1*12/2013    Wu 800/260
 2014//0298537  A1  10/2014    Mitchum et al.     

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     * cited by examiner
 
     Primary Examiner —Medina A Ibrahim
     Assistant Examiner —Wayne Zhong
     Art Unit — 1662
     Exemplary claim number — 1
 
(74)Attorney, Agent, or Firm — Thompson Coburn LLP; J. Wendy Davis

(57)

Abstract

Methods of inhibiting plant parasitic nematodes, methods of obtaining transgenic plants useful for inhibiting such nematodes, and transgenic plants that are resistant to plant parasitic nematodes through inhibition of plant nematode CLAVATA3/ESR (CLE) peptide receptor genes are provided. Methods for expressing genes at plant parasitic nematode feeding sites with plant nematode CLE peptide receptor gene promoters are also provided, along with nematode CLE peptide receptor gene promoters that are useful for expressing genes in nematode feeding sites as well as transgenic plants and nematode resistant transgenic plants comprising the promoters.
16 Claims, 19 Drawing Sheets, and 19 Figures


CROSS REFERENCE TO RELATED APPLICATIONS

[0001] This application is a National Stage application of International Patent Application No PCT/US2011/043882, filed Jul. 13, 2011 and incorporated herein by reference in its entirety, and claims the benefit under 35 USC § 119(e) of U.S. Provisional Application Ser. No, 61/371,619, filed Aug 6, 2010, which is incorporated herein by reference in its entirety.

STATEMENT REGARDING GOVERNMENTAL SUPPORT

[0002] This invention was made with Government support under Grant Numbers 2007-35607-17790, 2008-34113-19420, 2009-35302-05304, 2005-35604-15434, and 2008-35302-18824, all awarded by the USDA-NRI. The government has certain rights to this invention.

INCORPORATION OF SEQUENCE LISTING

[0003] The sequence listing that is contained in the file named “52553_111240_ST25.txt ”, which is 312,382 bytes in size (measured in operating system MS-Windows), created on Feb. 4, 2013, is filed herewith by electronic submission and incorporated herein by reference in its entirety.

BACKGROUND

[0004] Obligate biotrophs are pathogens that establish intimate parasitic relationships with the host that they infect. Often times these relationships involve some kind of modification or reprogramming of the host cell(s) to accommodate the pathogen's subsequent growth and development. Plant-parasitic nematodes are obligate biotrophs that mainly attack the roots of plants and cause over $100 billion in crop damage annually (Sasser and Freckman, 1987). The most economically important plant-parasitic nematodes include the cyst forming nematodes of Heterodera and Globodera spp. These sedentary endoparasitic nematodes form intimate parasitic relationships with their hosts by penetrating the root as motile juveniles and migrating intracellularly until they reach the root vasculature where they select a single cell to initiate a feeding site. The initial syncytial cell undergoes developmental changes to re-differentiate into a syncytium to support subsequent nematode growth and development in later sedentary stages (Davis et al., 2004). The syncytium forms when neighboring cells fuse as a result of partial cell wall degradation (Endo, 1964), creating a permanent feeding cell that shares characteristics with plant cell types including meristematic cells, endosperm cells, transfer cells, and developing xylem (Mitchum et al., 2008). It has been proposed that the development and maintenance of the syncytium is dependent on the secretory effector proteins originating in the esophageal gland cells and delivered into the host root through the stylet of plant-parasitic nematodes (Davis et al., 2008). Recently, the cyst nematode secreted CLAVATA3/ESR(CLE)-like effector proteins have been shown to act as ligand mimics of plant CLE peptides, and are required for successful nematode infection (Wang et al., 2005; Patel et al., 2008; Lu et al., 2009; Wang et al., 2010a; Wang et al., 2010b).
[0005] Plant CLEs are small peptide ligands involved in regulating a population of specialized cells, called stem cells, which allow postembryonic organogenesis to occur (Simon and Stahl 2006). These stem cell pools can be found in the shoot apical meristem (SAM), the root apical meristem (RAM), and the vascular cambium. Whether or not these stems cells remain in an undifferentiated state or differentiate into new plant tissues is tightly controlled by CLE signaling pathways. In Arabidopsis, the population of stem cells which resides in the organizing center (OC) of the SAM is maintained by the expression of the transcription factor WUSCHEL (WUS) (Laux et al., 1996). Differentiation of those stems cells is promoted when the ligand-receptor pair of CLAVATA3 (CLV3), a small extracellular peptide ligand in the CLE family (Fletcher et al., 1999; Rojo et al., 2002), binds to CLV1 (Ogawa et al., 2008), a leucine-rich-repeat receptor like kinase (LRR-RLK) and downregulates WUS. Previous models have suggested that CLV1 forms a receptor complex with the LRR-receptor like protein (RLP) CLV2 (Clark et al., 1993; Kayes and Clark, 1998; Jeong et al., 1999; Trotochaud et al., 1999). More recently, it has been suggested that CLV1 acts in parallel or together with the heterodimer receptor complex of CLV2 and CORYNE (CRN) (Miwa et al., 2008; Muller, 2008; Bleckmann et al., 2010; Zhu et al., 2010). In comparison to the SAM, much less is known about the regulation of the stem cells in the RAM. The quiescent center (QC) is the equivalent to the OC in the SAM. However, there are significant differences between the OC and the QC. In contrast to the OC, the cells surrounding the QC are maintained as stem cells. In addition, stem cells are differentiated in both proximal and distal directions. This indicates that there is a signaling ligand involved in cell-cell communication to maintain the cells surrounding the QC as stem cells, and a signal to promote differentiation (Sarkar et al., 2007; Stahl et al., 2009). Previous reports have identified that the WUS-related homeobox 5 (WOX5) transcription factor is expressed in the QC of the RAM and is required to maintain the distal stem cell pool (Sarkar et al., 2007). Recently it has been shown that CLE40, the closest homolog to CLV3, is expressed in the columella cells and regulates expression of WOX5 (Stahl et al., 2009). The WOX5/CLE40 signaling pathway appears to only control the distal stem cell pool, indicating that other CLE signaling pathways may exist to control the proximal stem cell pool. Consistent with these observations, a number of Arabidopsis CLEs are expressed in roots (Sharma et al., 2003), and when some of these CLEs are overexpressed they have been shown to cause premature termination of the primary root meristem (Fiers et al., 2004; Strabala et al., 2006; Meng et al., 2010). In addition, the short root phenotype has been shown to be dependent on CLV2 and CRN perception (Casamitjana-Martinez et al., 2003; Fiers et al., 2005; Miwa et al., 2008; Meng et al., 2010). Taken together this indicates that a CLV-like and CLE-controlled signaling pathway can act in the root.
[0006] CLE-like genes from nematodes have been reported in the soybean cyst nematode (SCN, H. glycines) (Wang et al., 2005; Wang et al., 2010a), the beet cyst nematode (BCN, H. schachtii) (Patel et al., 2008; Wang et al., 2010b), and the potato cyst nematode (PCN, G. rostochiensis) (Lu et al., 2009). BCN CLEs have been detected in the dorsal gland ampulla indicating they are likely secreted from the stylet into host cells (Patel et al., 2008). More recently, SCN CLEs have been shown to be secreted directly to the syncytial cytoplasm where the variable domain is thought to redirect the nematode CLE peptides to the apoplast (Wang et al., 2010a). These findings suggest that when delivered to the apoplast, nematode CLEs would be available to interact with extracellular receptors to function as ligand mimics of plant CLE signaling pathways. Overexpression studies have shown that nematode CLEs can trigger plant CLE signaling pathways (Wang et al., 2005; Lu et al., 2009; Wang et al., 2010a; Wang et al., 2010b), but the identity of the receptors and downstream signaling pathways that are activated to initiate developmental cascades required for the re-differentiation of root cells to form syncytia, are currently unknown.
[0007] US Patent Applications 20090077687 and 20090012029, identified nematode parasitism (effector) genes and described potential mechanisms to disrupt their expression and the function of their products to inhibit nematode parasitism of plants.

SUMMARY OF INVENTION

[0008] This invention provides for methods of inhibiting plant parasitic nematodes, methods of obtaining transgenic plants useful for inhibiting such nematodes, methods for expressing genes at plant parasitic nematode feeding sites, and transgenic plants that are resistant to plant parasitic nematodes. Also provided are promoters including, but not limited to a BAM1 promoter, that are useful for expressing genes in nematode feeding sites as well as transgenic plants and nematode resistant transgenic plants comprising the same. It is anticipated that the BAM1 and other promoters provided herewith can in certain embodiments be operably linked to genes that provide for inhibition of plant parasitic nematodes when introduced into transgenic plants and for plants that display such inhibition. Such genes that provide for inhibition of plant parasitic nematodes that can be used with the promoters provided herewith are disclosed in US Patent Application 20090012029, which is specifically incorporated herein by reference in its entirety.
[0009] In certain embodiments, a method for inhibiting plant parasitic nematode damage to a plant comprising growing a plant comprising a mutation or a transgene that provides for inhibition of at least one endogenous plant gene encoding a receptor for a nematode CLE peptide in the presence of plant parasitic nematodes is provided. In certain embodiments of these methods, the plant gene encoding a receptor for a nematode CLE peptide is selected from the group consisting of a CLV1-like gene, a CLAVATA2-like (CLV2-like) gene, a BAM1-like gene, a BAM2-like gene, a CRN-like gene, a ACR4-like gene, an ER-like gene, and an ERL2-like gene. In certain embodiments of these methods, CLV1-like gene, said CLV2-like gene, BAM1-like gene, a BAM2-like gene, a CRN-like gene, a ACR4-like gene, an ER-like gene, or an ERL2-like gene is an ortholog of a corresponding Arabidopsis, soybean, or potato CLV1, CLV2, BAM1, BAM2, CRN, ACR4, ER, or ERL2 gene. In certain embodiments of these methods, the methods can further comprise the step of harvesting a product of said plant. In certain embodiments of these methods, the harvested product is a leaf, stem, flower, seed, root, or tuber. In certain embodiments of these methods, the yield and/or quality of said product is increased relative to a control plant that is grown in presence of plant parasitic nematodes and that lacks said mutation or said transgene that provides for inhibition of at least one endogenous plant gene encoding a receptor for a nematode CLE peptide. In certain embodiments of these methods, the transgene comprises: i) an siRNA directed against said plant gene; ii) an artificial microRNA targeting said plant gene; iii) a dominant negative form of said plant gene; iv) an antisense or sense form of said plant gene; or v) a genomic insertion that disrupts said plant gene.
[0010] In certain embodiments, a method for obtaining a transgenic plant that exhibits resistance to a plant parasitic nematode comprising the steps of: a) introducing a transgene that provides for inhibition of at least one endogenous plant gene encoding a receptor for a nematode CLE peptide into a plant cell or a transgene that provides for inhibition of at least one CLV1-like, a CLV2-like, a BAM1-like, a BAM2-like, a CRN-like, a ACR4-like, an ER-like, and/or an ERL2-like gene; and b) selecting a transgenic plant obtained from said plant cell, wherein said selected transgenic plant comprises said transgene and exhibits resistance to a plant nematode is provided. In certain embodiments of these methods, CLV1-like gene, said CLV2-like gene, BAM1-like gene, a BAM2-like gene, a CRN-like gene, a ACR4-like gene, an ER-like gene, or an ERL2-like gene is an ortholog of a corresponding Arabidopsis, soybean, or potato CLV1, CLV2, BAM1, BAM2, CRN, ACR4, ER, or ERL2 gene.
[0011] In certain embodiments, a method for obtaining a transgenic plant expressing a gene product at a plant parasitic nematode feeding site, comprising the steps of: a) introducing a transgene wherein a CRN, CLV, or BAM promoter is operably linked to a gene encoding said gene product into a plant cell; and, b) selecting a transgenic plant obtained from said plant cell, wherein said selected transgenic plant comprises said transgene and exhibits expression of said gene product at said nematode feeding site is provided. In certain embodiments of these methods, the gene product is inhibitory to the plant parasitic nematode. In certain embodiments of these methods, the inhibitory gene product is a siRNA directed against a plant parasitic nematode gene. In certain embodiments of these aforementioned methods, an ACR4, BAM1, BAM2, CLV1, CLV2, CRN, ER, or ERL2 promoter is operably linked to a gene encoding the gene product. In certain embodiments of these aforementioned methods, an ACR4, BAM1, BAM2, CLV1, CLV2, CRN, ER, or ERL2 promoter is operably linked to a gene product that is inhibitory to a plant parasitic nematode. In certain embodiments of these methods, the inhibitory gene product is an amiRNA directed against a plant parasitic nematode gene.
[0012] In certain embodiments of any of the aforementioned methods of inhibiting plant parasitic nematode damage, obtaining a transgenic plant that exhibits resistance to a plant parasitic nematode, or obtaining a transgenic plant expressing a gene product at a plant parasitic nematode feeding site, the plant nematode is a cyst nematode. In certain embodiments of these methods, the cyst nematode is a Heterodera or Globodera spp. In certain embodiments of these methods, the Heterodera spp. is H. avenae, H. bifenestra, H cajani. H. carotae, H. ciceri, H. cruciferae, H. cynodontis, H. cyperi, H. davert, H. elachista, H. fii, H. galeopsidis, H. goettingiana, H. graminis, H. hordecalis, H. humuli, H. iri, H. latipons, H. lespedeza, H. leucilyma, H. Iongicaudata, H. mani, H. maydis, H. medicaginis, H. oryzae, H. oryzicola, H. sacchari, H. salixophila, H. schachtii, H. sorghii, H. trifoii, H. urticae, H. vigna, or H. zeae. In certain embodiments of these methods, the Globodera spp. is G. achilleae, G. artemisiae, G. hypolysi, G. leptonepia, G. mali, G. pallida, G. rostochiensis, G. tabacum, or G. zeylandica.
[0013] In certain embodiments of any of the aforementioned methods of inhibiting plant parasitic nematode damage, obtaining a transgenic plant that exhibits resistance to a plant parasitic nematode, or obtaining a transgenic plant expressing a gene product at a plant parasitic nematode feeding site, the plant is a monocot or dicot plant, or is selected from the group consisting of a tobacco, cereal, sugar beet, cotton, fruit, fiber, oilseed, potato, rice, corn, soybean, vegetable, and wheat plant.
[0014] In certain embodiments of any of the aforementioned methods of inhibiting plant parasitic nematode damage or obtaining a transgenic plant that exhibits resistance to a plant parasitic nematode, the endogenous plant gene encoding a receptor for a nematode CLE is a potato StCLV1, StCLV2, StBAM1, StBAM2, StCRN, StACR4, StER, or StERL2 gene and the plant is a potato plant. In certain embodiments of these methods, the plant parasitic nematode is G. rostochiensis or G. pallida.
[0015] In certain embodiments of any of the aforementioned methods of inhibiting plant parasitic nematode damage or obtaining a transgenic plant that exhibits resistance to a plant parasitic nematode, the endogenous plant gene encoding a receptor for a nematode CLE is selected from the group consisting of soybean genes provided in Table 3 of Example 2 and said plant is a soybean plant. In certain embodiments of any of the aforementioned methods of inhibiting plant parasitic nematode damage, the plant parasitic nematode is Heterodera glycines or H. schachtii.
[0016] In certain embodiments, a plant parasitic nematode resistant transgenic plant comprising a transgene that provides for inhibition of at least one endogenous plant gene encoding a receptor for a nematode CLE peptide is provided. In certain embodiments, the transgene comprises: i) an siRNA directed against said plant gene; ii) an artificial microRNA targeting said plant gene; iii) a dominant negative form of said plant gene; iv) an antisense or sense form of said plant gene; or v) a genomic insertion that disrupts said plant gene. In certain embodiments of any of the aforementioned transgenic plants, the endogenous plant gene encoding a receptor for a nematode CLE is selected from the group consisting of soybean genes of provided in Table 3 of Example 2 and the plant is a soybean plant. In certain embodiments of any of the aforementioned transgenic plants, the endogenous plant gene encoding a receptor for a nematode CLE is a potato StCLV1, StCLV2, StBAM1, StBAM2, StCRN, StACR4, StER, or StERL2 gene and the plant is a potato plant.
[0017] In certain embodiments, a plant parasitic nematode resistant transgenic plant comprising a transgene wherein a CRN, CLV, or BAM promoter is operably linked to a gene encoding a gene product that is inhibitory to a plant parasitic nematode is provided. In certain embodiments, the gene product is an siRNA directed against a plant parasitic nematode gene. In certain embodiments of any of the aforementioned plants, the CRN, CLV, or BAM promoter is the CRN1, CLV2, or BAM1 promoter sequence provided in Example 3. In certain embodiments of these aforementioned methods, an ACR4, BAM1, BAM2, CLV1, CLV2, CRN, ER, or ERL2 promoter is operably linked to a gene product that is inhibitory to a plant parasitic nematode. In certain embodiments, the gene product is a siRNA or an amiRNA directed against a plant parasitic nematode gene.
[0018] In certain embodiments, a recombinant DNA construct comprising a BAM1 promoter that is operably linked to a heterologous gene, wherein said BAM1 promoter comprises any one of: i) the BAM1 promoter sequence provided in Example 3; ii) a promoter that has at least 70%, 85%, 90%, 95%, or 99% sequence identity to the BAM1 promoter sequence provided in Example 3; or ii) a promoter comprising a deletion of about up to about 10, 50, 100, 200, 500, 700, 1000, or 1500 nucleotides of the 5′ nucleotides of the BAM1 promoter sequence provided in Example 3 is provided. In certain embodiments, the BAM promoter is operably linked to a gene encoding a gene product that is inhibitory to a plant parasitic nematode.

DESCRIPTIONS OF THE FIGURES

Figure Legends

[0019] FIG. 1. Effect of cyst nematode CLE peptides on receptor mutants.
[0020] (a) Average root length wild-type (Ler), clv2-1, and crn-1 seedlings grown for 9 days on media with or without the synthetic nematode dodecapeptide CLE motif Data represent the mean±SE, n=10. (b)-(d) Representative roots tips of seedlings grown on media with or without synthetic CLE peptides for 10 days and visualized with differential interference microscopy. (b) No peptide, (c) Sensitive to peptide, and (d) Resistant to peptide. (Scale bar, 50 μm).
[0021] FIG. 2. CRN:GUS expression during nematode infection. (a)-(c) GUS expression in uninfected Arabidopsis root tips (a), middle of the root (b), and older part of the root towards the hypocotyl (c). (d)-(g) CRN:GUS expression in response to H. schachtii; early parasitic J2 (d), late parasitic J2 (e), J3 parasitic (f), J4 parasitic (g). Abbreviations: nematode, N; Syn, Syncytium. (Scale bar, 50 μm).
[0022] FIG. 3. Confocal images of CLV2:H2B-mCherry expression during nematode infection. (a) J2 parasitic with DIC. (b) J2 parasitic with mCherry fluorescence. (c) J3 parasitic with DIC. (d) J3 parasitic with mCherry fluorescence. Abbreviations: nematode, N; Syn, Syncytium. (Scale bars, 50 μm).
[0023] FIG. 4. Effect of clv2-1 and crn-1 mutant alleles on H. schachtii infection.
[0024] (a) J4 females were counted at 14 dpi and adult females were counted at 30 dpi. Data represent mean±SE, n=35 for Ler, 32 for crn-1, 34 for clv2-1, and 29 for crn-1 clv2-1. Data are representative of three independent experiments.
[0025] (b) Seedlings were grown on vertical square plates for 10 days and inoculated with 10 J2s/root. At 14 dpi, syncytia that fed only one nematode and appeared translucent were microscopically examined and their area was determined. Data represent mean±SE, n=11 for Ler and crn-1, 14 for clv2-1, and 12 for crn-1 clv2-1.
[0026] Asterisks indicate statistically significant differences compared to Ler by Student's t test (P<0.05)
[0027] FIG. 5. Response of wild-type (Utr) and sol2-1 seedlings to the synthetic 12-aa nematode CLE peptide. Average root length wild-type (Utr) and sol2-1 seedlings grown for 9 days on media with or without the synthetic nematode dodecapeptide CLE motif. Data represent the mean ±SE, n =10.
[0028] FIG. 6. Confocal images of nematode autofluorescence in wild-type roots (a and b). Feeding site induced by a parasitic J2. (a) DIC image. (b) mCherry fluorescence. Abbreviations: nematode, N; Syn, Syncytium. (Scale bar 50 μm).
[0029] FIG. 7. Effect of sol2-1 mutant allele on Heterodera schachtii infection. (a). J4 females were counted at 14dpi and adult females were counted at 30 dpi. Data represent mean ±SE, n =36. Asterisks indicate statistically significant differences compared to Ler by Student's t test (P <0.0001). Effect of sol2-1 mutant allele on size of syncytia (b). Seedlings were grown on vertical square plates for 10 days and inoculated with 10 ppJ2s/root. At 14 dpi, syncytia that fed only one nematode and appeared translucent were microscopically examined and their area was determined. Data represent mean ±SE, n =11 for Utr and n =9 for sol2-1. Asterisks indicate statistically significant differences compared to Ler by Student's t test (P <0.05).
[0030] FIG. 8. Effect of Heterodera glycines (HgCle) and Heterodera schachtii (HsCLE) nematode CLE peptides on receptor mutants. Seedlings were grown for 9 days on media with or without the synthetic nematode dodecapeptide CLE motif. Data represent the mean ±SE, n =10.(A) Average root length of wild-type (Ler), clv2-1, and crn-1. (B) Average root length of wild-type (Col-0,) bam3-2, bam2, and bam1-3. (C) Average root length of wild-type (Utr) and sol2-1.
[0031] FIG. 9. Effect of Globodera rostochiensis (GrCLE) nematode CLE peptides on receptor mutants. Seedlings were grown for 9 days on media with or without the synthetic nematode dodecapeptide CLE motif. Data represent the mean ±SE, n =10. Average root length of wild-type (Col-0) bam1-2, bam1-4, erl2-1, and clv2-1.
[0032] FIG. 10. Effect of receptor mutant alleles on H. schachtii infection. J4 females were counted at 14dpi and adult females were counted at 30 dpi. Asterisks indicate statistically significant differences compared to wild-type by Student's t test (P <0.05). Data represent mean ±SE, Similar results were obtained from two additional biological replicates.
[0033] FIG. 11. CRN:GUS expression during nematode infection. (A-C) CRN: :GUS expression in uninfected Arabidopsis root tips (A), middle of the root (B), and older part of the root towards the hypocotyl (C). (D-G) CRN: :GUS expression in nematode-infected roots; (D) early J2p, (E) late J2p, (F) J3 and (G) J4 females. Abbreviations: nematode, N; Syn, Syncytium.
[0034] FIG. 12. CLV2:GUS expression during nematode infection. (A-D) Confocal section of a nematode induced feeding site 4 dpi (A-B) and 8 dpi (A-B) expressing CLV2:H2B-mCherry. Abbreviations: nematode, N; Syn, Syncytium.
[0035] FIG. 13. BAM1:GUS expression in Arabidopsis in response to nematode infection. (A) Uninfected root tip. (B-D) GUS expression in nematode infected roots; (B) Early parasitic J2, (C) Parasitic J3, and (D) Parasitic J4.
[0036] FIG. 14 Differential expression of candidate potato CLE receptor genes in G. rostochiensis-infected potato roots.
[0037] FIG. 15. Effect of crn-1, clv2-6, bam1-3 mutant alleles and combinations thereof on H. schachtii infection in Arabidopsis.
[0038] FIG. 16 shows the expression of a pCLV1 promoter fusion to a GUS gene in the vasculature of plants and upregulation at sites of H. schachtii in transgenic Arabidopsis.Abbreviations: nematode, N.
[0039] FIG. 17 shows a StCLV2 Potato Promoter:GUS transgenic plant line and activity of this promoter in G. rostochiensis-induced feeding sites.
[0040] FIG. 18 shows a StCRN Potato Promoter:GUS transgenic plant line and activity of this promoter in G. rostochiensis-induced feeding sites.
[0041] FIG. 19 A shows expression levels of the endogenous StCLV2 gene in transgenic potato plants expressing an artificial miRNA (amiRNA) directed against the StCLV2 gene (3d#29 and 4d#9) and wild type (Wt) control plants that lack the amiRNA.
[0042] FIG. 19 B shows the number of G. rostochiensis cysts in transgenic potato plants expressing an artificial miRNA (amiRNA) directed against the StCLV2 gene (3d#29 and 4d#9) and wild type (Wt) control plants that lack the amiRNA.

DESCRIPTION OF THE INVENTION

[0043] We describe the use of synthetic CLE peptides, nematode CLE overexpression lines, promoter-reporter lines, and nematode infection assays of receptor mutants to investigate a role for CLV2 and CRN in nematode CLE signaling. Our results indicate that the CLV2/CRN signaling pathway is required for successful nematode infection and syncytium development.
[0044] Plant-parasitic cyst nematodes secrete CLAVATA3 (CLV3)/ESR(CLE)-like effector proteins. These proteins have been shown to act as ligand mimics of plant CLE peptides and are required for successful nematode infection; however, the receptors for nematode CLE-like peptides have not been identified. Here we demonstrate that CLV2 and CORYNE (CRN), members of the receptor kinase family, are required for nematode CLE signaling. Exogenous peptide assays and overexpression of nematode CLEs in Arabidopsis showed that CLV2 and CRN are required for nematode CLE perception. In addition, promoter-reporter assays showed that both receptors are expressed in nematode-induced syncytia. Lastly, infection assays with receptor mutants revealed a decrease in both nematode infection and syncytia size. Taken together, our results indicate that nematode CLE perception by CLV2 and CRN is not only required for successful nematode infection, but is also involved in the formation or maintenance of nematode-induced syncytia.

Plant Nematode CLE Receptor Genes that can be Used to Obtain Nematode Resistant Plants and Methods of Use

[0045] A variety of plant nematode CLE peptide receptor genes (hereinafter referred to as “PNCLEPRG”) that provide for inhibition of plant parasitic nematode infections are provided herewith, along with associated methods of use, and plants comprising transgenes or mutations wherein expression of the PNCLEPRG are inhibited. Reduced expression of the PNCLEPRG in plants inhibits infection of the plants by nematodes. Such reductions in nematode infection result in improved plant yield and plant product quality.
[0046] Reductions in expression of the endogenous PNCLEPRG can be effected by any method that at least provides for reductions in the amount or activity of the PNCLEPRG at the site of nematode infection in the plant. Such sites of infection are commonly the plant roots, but can also comprise other plant parts such as tubers.
[0047] In certain embodiments, inhibition of PNCLEPRG expression in a plant can be effected by transgenes. Such transgenes include, but are not limited to, transgenes that: i) produce an siRNA directed against the PNCLEPRG; ii) produce an artificial microRNA targeting the PNCLEPRG; iii) produce a dominant negative form of the protein product of the PNCLEPRG; iv) produce an antisense or sense form of the PNCLEPRG; or v) comprise a genomic insertion that disrupts the endogenous PNCLEPRG.
[0048] Exemplary vector systems that can provide for production of siRNA in plants include, but are not limited to, vectors disclosed by Dafny-Yelin, et al. (Plant Physiology, 2007, Vol. 145, pp. 1272-1281), Wesley et al. 2001, Plant J. 27: 581-590, and Miki and Shimamoto, (2004) Plant Physiol 138: 1903-1913. Vectors for producing an siRNA are also described in U.S. Pat. No. 6,635,805, incorporated herein by reference in its entirety.
[0049] Exemplary vector systems that can provide for production of artificial miRNA in plants include, but are not limited to, vectors disclosed by Warthmann et al. (2008) PLoS ONE 3(3): e1829. doi:10.1371/journal.pone.0001829; and Alvarez et al. (2006) Plant Cell 18: 1134-1151. Vectors for effecting efficient inhibition of endogenous plant genes by expression of hairpin RNAs are also disclosed in U.S. Patent Application Nos. 20050164394, 20050160490, and 20040231016, each of which is incorporated herein by reference in their entirety. Exemplary dominant negative mutations that can provide for inhibition endogenous PNCLEPRG include, but are not limited, mutations modeled after dominant negative mutations in other Leucine Rich Repeat-Receptor Like Kinase (LRR-RLK) proteins.
[0050] In one embodiment, the dominant negative mutation can comprise a deletion or other loss-of-function mutation in the kinase domain. Such mutations have been disclosed for plant LRR-RLK proteins (Shpak et al., Plant Cell, Vol. 15, 1095-1110, 2003). Methods of identifying transgene insertions into specific genomic loci have also been disclosed. T-DNA of Agrobacterium is also an insertional mutagen that can be used as an agent to reduce expression of an endogenous PNCLEPRG. T-DNA mutagenesis has been described in Arabidopsis (Krysan et al., Plant Cell, 1999, 1: 2283-2290) and rice (Jeon et al., Plant J. June 2000; 22(6):561-70). Transposons such as those in the Ac/Ds (Activator-Disassociation) family and the Enhancer-inhibitor system can also be used to effect mutagenesis of an endogenous PNCLEPRG. Transposon mutagenesis schemes have been described (Speulman et al. Plant Cell, Vol. 11, 1853-1866, October 1999; Das, L., and Martienssen, R, 1995, Plant Cell 7:287-294).
[0051] Plants wherein expression of the endogenous PNCLEPRG is inhibited by a mutation and the use of such plants is also provided. Methods of identifying plants comprising mutations in PNCLEPRG include, but are not limited to, “TILLING” (Targeting Induced Local Lesions in Genomes). The TILLING technique comprises the induction of mutations across the genome followed by the identification and isolation of plants with mutations in desired genes (McCallum, Plant Physiology, 2000, Vol. 123, pp. 439-44).
[0052] PNCLEPRG target genes useful in the methods and plants of this invention include, but are not limited to, the ACR4, CLV1, CLV2, CRN, BAM1, BAM2, ER, and ERL2 genes of Arabidopsis and the orthologous ACR4, CLV1, CLV2, CRN, BAM1, BAM2, ER, and ERL2 genes of crop and ornamental plants subject to nematode infestation. Such orthologous genes are referred to herein as “ACR4-like, CLV1-like, CLV2-like, CRN-like, BAM1-like, BAM2-like, ER-like, and ERL2-like” genes. As used herein, the terms “orthologous” and “-like” (when appended to a gene) thus refer to genes that at least have a similar role in plant nematode CLE peptide signal transduction in their respective plant species of origin. In certain embodiments, the PNCLEPRG target genes are obtained from a plant that is a monocot or dicot plant, or that is a crop plant such as a tobacco, cereal, sugar beet, cotton, fruit, fiber, oilseed, potato, rice, corn, soybean, vegetable, and wheat plant. Exemplary vegetable plants include, but are not limited to, carrot, pepper, cucurbit, and tomato plants.
[0053] In certain embodiments, the PNCLEPRG target genes are derived from the plant that will be used (i.e. protected from nematode infection). However, a PNCLEPRG of a given plant specie can be used in a distinct plant species when it has sufficient homology to the orthologous PNCLEPRG of a distinct plant species. In this context, “sufficient homology” is that amount of homology necessary to provide for transgene-mediated inhibition of the orthologous gene. For certain transgene-mediated gene inhibition methods, a PNCLEPRG sequence of about is 23 nucleotides or longer with least 80%, 85%, 90%, 95%, 98%, 99% or 100% identity to the target orthologous sequence can be used. In certain embodiments, a hairpin RNA may comprise a 5′ sequence of roughly 19-24 nucleotides of sense strand target gene sequence with 100% identity followed by a spacer nucleotide of about 8-10 nucleotides followed by a sequence of roughly 19-24 nucleotides of antisense sequence that is capable of base pairing with the preceding sense strand sequence. In certain embodiments, a 19-24 base region of a PNCLEPRG that exhibits 100% identity over 19-24 nucleotides to an orthologous PNCLEPRG can also be used to inhibit that orthologous gene.
[0054] In certain embodiments, an Arabidopsis PNCLEPRG can be used to obtain nematode resistant plants, where the plants are Arabidopsis or other plants that comprise orthologous PNCLEPRGs that can be inhibited by the Arabidopsis PNCLEPRG. Arabidopsis PNCLEPRG include, but are not limited to, the ACR4, CLV1, CLV2, CRN, BAM1, BAM2, ER, and ERL2 can in certain embodiments be used to control plant parasitic nematode infections of cruciferous plants that include, but are not limited to, arugula, cauliflower, cabbage, cress, bok choy, broccoli, radish, canola, turnip, watercress, and the like.
[0055] In certain embodiments, a potato PNCLEPRG can be used to obtain nematode resistant plants, where the plants are potato plants or other plants that comprise orthologous PNCLEPRGs that can be inhibited by the potato PNCLEPRG. Potato PNCLEPRG provided herein include, but are not limited to, stCRN (SEQ ID NO:6), stBAM1 (SEQ ID NO:7), stBAM2 (SEQ ID NO:8), stER (SEQ ID NO:9), stCLV1 (SEQ ID NO:10), stCLV2 (SEQ ID NO:11), stACR4 (SEQ ID NO:12), and stERL2 (SEQ ID NO:13). Also provided herewith are related sequences with at least 70%, 80%, 85%, 90%, 95%, 97%, 98%, or 99% identity to stCRN (SEQ ID NO:6), stBAM1 (SEQ ID NO:7), stBAM2 (SEQ ID NO:8), stER (SEQ ID NO:9), stCLV1 (SEQ ID NO:10), stCLV2 (SEQ ID NO:11), stACR4 (SEQ ID NO:12), and stERL2 (SEQ ID NO:13) as well as methods of using such sequences to control plant nematodes.
[0056] In certain embodiments, the use of such potato PNCLEPRGs and related sequences to control plant nematode, and particularly, plant cyst nematode infections, in solanaceous plants including, but not limited to, eggplant, tobacco, potato, and tomato is provided. In certain embodiments, the use of such potato PNCLEPRGs and related sequences to control Globedera sp. infections of potato plants is provided. In any of the aforementioned embodiments, inhibition of the plant PNCLEPRG can be limited to inhibition in roots or limited to inhibition at the site of nematode infection by use of root-specific and/or nematode inducible promoters, respectively.
[0057] In certain embodiments, a soybean PNCLEPRG can be used to obtain nematode resistant plants, where the plants are soybean plants or other plants that comprise orthologous PNCLEPRGs that can be inhibited by the soybean PNCLEPRG. Soybean PNCLEPRG provided herein include, but are not limited to, soybean CRN (SEQ ID NO:44, 45, 47, and 48), BAM1 (SEQ ID NO:23, 24, 26, 27), BAM2 (SEQ ID NO:29, 30, 32, 33), CLV1 (SEQ ID NO:38, 39, 41, 42), and CLV2 (SEQ ID NO:35, 36, 50, 51) orthologs. Also provided herewith are related sequences with at least 70%, 80%, 85%, 90%, 95%, 97%, 98%, or 99% identity to soybean CRN (SEQ ID NO:44, 45, 47, 48), BAM1 (SEQ ID NO:23, 24, 26, 27), BAM2 (SEQ ID NO:29, 30, 32, 33), CLV1 (SEQ ID NO:38, 39, 41, 42), and CLV2 (SEQ ID NO: 35, 36, 50, 51) orthologs as well as methods of using such sequences to control plant nematodes. In certain embodiments, the use of such soybean PNCLEPRGs and related sequences to control plant nematodes, and particularly, plant cyst nematode infections, in leguminous plants including, but not limited to, alfalfa, clover, peas, beans, lentils, lupins, mesquite, carob, soybean, and peanuts, is provided. In certain embodiments, the use of such soybean PNCLEPRGs and related sequences to control Heterodera glycine infections of soybean plants is provided. In any of the aforementioned embodiments, inhibition of the plant PNCLEPRG can be limited to inhibition in roots or limited to inhibition at the site of nematode infection by use of root-specific and/or nematode inducible promoters, respectively.
[0058] In certain embodiments of the invention, combinations of two or more a plant PNCLEPRG are inhibited in a plant to provide resistance to plant parasitic nematode infections. Plants wherein combinations of two or more PNCLEPRG selected from the group of CRN, BAM1, BAM2, ER, CLV1, CLV2, ACR4, and ERL2 genes or orthologs thereof are inhibited can be used to provide resistance to plant parasitic nematode infections. In certain embodiments, a plant CLV2-like and a plant BAM1-like gene are both inhibited in parallel to reduce nematode infections in the plant. In certain embodiments of the invention, a plant CRN-1-like and a plant BAM1-like gene are both inhibited in parallel to reduce nematode infections in the plant. In certain embodiments, combinations of two or more of a soybean CRN (SEQ ID NO:44, 45, 47, and 48), BAM1 (SEQ ID NO:23, 24, 26, 27), BAM2 (SEQ ID NO:29, 30, 32), CLV1 (SEQ ID NO:38, 39, 41, 42), and CLV2 (SEQ ID NO:35, 36, 50, 51) orthologs or a related sequence are inhibited in a soybean or other plant to provide resistance to plant parasitic nematode infections. In certain embodiments, combinations of two or more of a potato stCRN (SEQ ID NO:6), stBAM1 (SEQ ID NO:7), stBAM2 (SEQ ID NO:8), stER (SEQ ID NO:9), stCLV1 (SEQ ID NO:10), stCLV2 (SEQ ID NO:11), stACR4 (SEQ ID NO:12), and stERL2 (SEQ ID NO:13) are inhibited in a potato or other plant to provide resistance to plant parasitic nematode infections. In any of the aforementioned embodiments, inhibition of the plant PNCLEPRG can be limited to inhibition in roots or limited to inhibition at the site of nematode infection by use of root-specific and/or nematode inducible promoters, respectively.
[0059] In addition to nematode resistant plants, the instant invention also provides for parts of those plants and plant cells. Plant parts provided herein include, but are not limited to, seeds, tubers, roots, leaves, stalks, lint, and the like. Also provided herein are processed products of the nematode resistant plants. Such processed plant products include, but are not limited to, a ground meal, a feed, a cake, and the like. In certain embodiments, such processed product would comprise a detectable amount of a transgene used to inhibit the PNCLEPRG.

Promoters from Plant Nematode CLE Receptor Genes and Methods of Use

[0060] Promoters from PNCLEPRG and recombinant DNA constructs providing such promoters that are useful for expressing genes of interest in plant cells where the nematodes feed are provided. Such promoters are particularly useful for expressing nucleic acid and/or protein sequences that are inhibitory to plant parasitic nematodes. Particular advantages of the promoters include, but are not limited to, providing for expression of the operably linked nucleic acid sequences at nematode feeding sites within the plant while limiting expression of the gene in other parts of the plant where such expression is not required or desired. As used herein in the context of a promoter, the term “operably linked” means that a promoter is connected to a sequence of interest such that the transcription of that sequence of interest is controlled and regulated by that promoter. When the sequence of interest encodes a protein and when expression of that protein is desired, “operably linked” means that the promoter is linked to the sequence in such a way that the resulting transcript will be efficiently translated. If the linkage of the promoter to the coding sequence is a transcriptional fusion and expression of the encoded protein is desired, the linkage is made so that the first translational initiation codon in the resulting transcript is the initiation codon of the coding sequence. Alternatively, if the linkage of the promoter to the coding sequence is a translational fusion and expression of the encoded protein is desired, the linkage is made so that the first translational initiation codon contained in the 5′ untranslated sequence associated with the promoter and is linked such that the resulting translation product is in frame with the translational open reading frame that encodes the protein desired.
[0061] A variety of recombinant DNA molecules comprising promoters of the invention that are operably linked to heterologous genes or nucleic acids of interest are provided. Heterologous genes or nucleic acids that provide for inhibition of plant parasitic nematodes can be operably linked to the PNCLEPRG promoters. In certain embodiments, the heterologous genes or nucleic acids of interest provide for inhibition of a plant parasitic nematode gene or function. Such plant parasitic nematode genes or functions include, but are not limited to, nematode genes that are essential or required for nematode viability or nematode genes involved in any aspect of plant host parasitism. In certain embodiments, the promoters are used to drive expression of heterologous genes or nucleic acids that are inhibitory to nematode genes disclosed in US Patent Application publication US20090012029, which discloses inhibitory nucleic acid specific for one or more cyst nematode esophageal gland cell proteins and which is incorporated herein by reference in its entirety.
[0062] In certain embodiments, the promoters are used to drive expression of genes or nucleic acids that inhibit formation and/or maintenance of the plant cells of the nematode feeding site. In certain embodiments, the promoters are thus used to: i) drive expression of heterologous genes or nucleic acids that are inhibitory to endogenous plant genes involved in formation and/or maintenance of the plant cells of the nematode feeding site; and/or, ii) drive expression of heterologous genes that comprise endogenous plant genes that are downregulated during the formation and/or maintenance of the plant cells of the nematode feeding site. Endogenous plant genes involved in formation and/or maintenance of the plant cells of the nematode feeding site that include, but are not limited to, genes involved in the cell wall architectural modifications during feeding site formation/maintenance, genes involved in sugar or carbohydrate, metal ion, and amino acid transport, and genes involved in plant phytohormone signaling and biosynthesis. A variety of soybean plant genes suitable for use with the promoters of the invention are disclosed in Ithal et al., Molec. Plant. Microb. Interact. Vol. 20, No. 5, 2007, pp. 510-525, incorporated herein by reference in its entirety. PNCLEPRG promoters useful in the methods and plants of this invention include, but are not limited to, the ACR4, CLV1, CLV2, CRN, BAM1, BAM2, ER, and ERL2 promoters of Arabidopsis and the orthologous ACR4, CLV1, CLV2, CRN, BAM1, BAM2, ER, and ERL2 promoters of crop and ornamental plants subject to nematode infestation. Such orthologous promoters are referred to herein as “ACR4-like, CLV1-like, CLV2-like, CRN-like, BAM1-like, BAM2-like, ER-like, and ERL2-like” promoters. As used herein, the terms “orthologous” and “-like” (when appended to a promoter) thus refer to promoters that at least have a similar role or expression pattern in plant nematode CLE peptide signal transduction in their respective plant species of origin. In certain embodiments, the PNCLEPRG promoters are obtained from a plant that is a monocot or dicot plant, or that is a crop plant such as a tobacco, cereal, sugar beet, cotton, fruit, fiber, oilseed, potato, rice, corn, soybean, vegetable, and wheat plant. Exemplary vegetable plants include, but are not limited to, carrot, pepper, cucurbit, and tomato plants.
[0063] In certain embodiments, a recombinant DNA construct comprising a PNCLEPRG promoter that is operably linked to a heterologous gene, or a plant, plant cell, plant part, or processed plant product comprising the same, is provided. In certain embodiments, the PNCLEPRG promoter comprises any one of: i) a potato ACR4 promoter (SEQ ID NO:15), an Arabidopsis (SEQ ID NO:14), potato (SEQ ID NO:16), or soybean (SEQ ID NO: 38 or 41) CLV1 promoter; ii) an Arabidopsis (SEQ ID NO:4), potato (SEQ ID NO:17), or soybean (SEQ ID NO:35 or 50) CLV2 promoter; iii) an Arabidopsis (SEQ ID NO:5), potato (SEQ ID NO:18), or soybean (SEQ ID NO: 44 or 47) CRN promoter; iv) an Arabidopsis (SEQ ID NO:3), potato (SEQ ID NO:19), or soybean (SEQ ID NO: 23 or 26) BAM1 promoter; v) a potato (SEQ ID 20), or soybean (SEQ ID NO: 29 or 32) BAM2 promoter; vi) a potato ER promoter (SEQ ID NO:21); vii) or a potato ERL2 promoter (SEQ ID NO:22). Also provided are recombinant DNA constructs comprising a variant PNCLEPRG promoter that has at least 70%, 85%, 90%, 95%, or 99% sequence identity to any one of: i) a potato ACR4 promoter (SEQ ID NO:15), an Arabidopsis (SEQ ID NO:14), potato (SEQ ID NO:16), or soybean (SEQ ID NO: 38 or 41) CLV1 promoter; ii) an Arabidopsis (SEQ ID NO:4), potato (SEQ ID NO:17), or soybean (SEQ ID NO:35 or 50) CLV2 promoter; iii) an Arabidopsis (SEQ ID NO:5), potato (SEQ ID NO:18), or soybean (SEQ ID NO: 44 or 47) CRN promoter; iv) an Arabidopsis (SEQ ID NO:3), potato (SEQ ID NO:19), or soybean (SEQ ID NO: 23 or 26) BAM1 promoter; v) a potato (SEQ ID 20), or soybean (SEQ ID NO: 29 or 32) BAM2 promoter; vi) a potato ER promoter (SEQ ID NO:21); vii) or a potato ERL2 promoter (SEQ ID NO:22).
[0064] In certain embodiments, recombinant DNA constructs comprising a PNCLEPRG promoter comprising a deletion of about up to about 10, 50, 100, 200, 500, 700, 1000, or 1500 nucleotides of the 5′ nucleotides of any one of: i) a potato ACR4 promoter (SEQ ID NO:15), an Arabidopsis (SEQ ID NO:14), potato (SEQ ID NO:16), or soybean (SEQ ID NO: 38 or 41) CLV1 promoter; ii) an Arabidopsis (SEQ ID NO:4), potato (SEQ ID NO:17), or soybean (SEQ ID NO:35 or 50) CLV2 promoter; iii) an Arabidopsis (SEQ ID NO:5), potato (SEQ ID NO:18), or soybean (SEQ ID NO: 44 or 47) CRN promoter; iv) an Arabidopsis (SEQ ID NO:3), potato (SEQ ID NO:19), or soybean (SEQ ID NO: 23 or 26) BAM1 promoter; v) a potato (SEQ ID 20), or soybean (SEQ ID NO: 29 or 32) BAM2 promoter; vi) a potato ER promoter (SEQ ID NO:21); vii) or a potato ERL2 promoter (SEQ ID NO:22) is provided. Those skilled in the art will appreciate that promoter and 5′UT regions of PNCLEPRG provided herewith as genomic sequences in association with the coding regions can be dissociated from those coding regions and operably linked to heterologous nucleic acids or genes by transcriptional or translational fusions. In certain embodiments, the soybean PNCLEPRG promoters and 5′UT of Table 5 (SEQ ID NO: 23, 26, 29, 32, 35, 38, 41, 44, 47, and 50) thus comprise the nucleic acid sequences located 5′ to the start codon of those genomic sequences.
[0065] In certain embodiments, variants of any of the aforementioned PNCLEPRG promoters comprising at least about 300, 500, 800, 900, 1,000, 1,500, 2,500, or 3,000 nucleotides of the nucleic acid sequence located 5′ to the start codon or located 5′ to mRNA 5′ cap site of the endogenous gene associated with said promoter are provided. Also provided are recombinant DNA constructs wherein any of the aforementioned promoters is operably linked to a gene encoding a gene product that is inhibitory to a plant parasitic nematode.
[0066] In addition to nematode resistant plants comprising the recombinant DNA constructs of the aforementioned PNCLEPRG promoters, the instant invention also provides for parts of those plants and plant cells. Plant parts provided herein include, but are not limited to, seeds, tubers, roots, leaves, stalks, lint, and the like. Also provided herein are processed products of the nematode resistant plants. Such processed products include, but are not limited to, a ground meal, a feed, a cake, and the like.
[0067] In certain embodiments, such processed product would comprise a detectable amount of a recombinant DNA comprising a PNCLEPRG promoter that is operably linked to a heterologous gene.

EXAMPLES

[0068] The disclosed embodiments are merely representative of the invention, which may be embodied in various forms. Thus, specific structural and functional details disclosed herein are not to be interpreted as limiting.

Example 1

Experimental Procedures

Peptide Assays

[0069] Arabidopsis seeds were sterilized using the chlorine gas method (Wang et al., 2010b). Sterilized seeds were germinated on vertical plates in a growth chamber at 22° C. under long-day conditions (16 h light/8 h dark) containing synthetic peptides (Sigma-Genosys) as previously described (Wang et al., 2010b). The clv2-1 mutant in the Ler background (Koornneef et al., 1983) was obtained from the Arabidopsis Biological Resource Center. The crn-1 mutant in the Ler background (Muller, 2008) and the sol2-1 mutant in the Utr background (Miwa et al., 2008) have been described previously. The HgCLEp, HsCLE1p, and HsCLE2p peptides used in this study were as described (Wang et al., 2010b). Two days after germination, root length was marked each day for nine days. Plates were scanned using an Epson Perfection V200 PHOTO scanner and total root length was determined using Scion Image. Primary root tips of Arabidopsis were mounted on glass slides and visualized with an Olympus Vanox AHBT3 microscope equipped with Nomarski optics.

Overexpression in Mutant Backgrounds

[0070] The CLE gene sequences from the soybean cyst nematode (HgCLE2ΔSP) and the beet cyst nematode (HsCLE1 and HsCLE2) used to generate the overexpression constructs were previously described (Wang et al., 2010a; Wang et al., 2010b). Constructs were transformed into the mutant backgrounds using the Arabidopsis floral dip method (Clough and Bent, 1998). Seeds from primary Arabidopsis transformants (T1) were selected on 0.5×MS media [MS basal nutrients salts (Caisson Laboratories), 2% sucrose, 0.8% Type A agar (Sigma), pH 5.7] containing 50 μg/mL timentin (GlaxoSmithKline) to control Agrobacterium contamination, and 50 μg/mL kanamycin and grown under the same conditions as above. Seedlings resistant to kanamycin were transplanted to soil seven days after germination. Two weeks after transplanting to soil the shoot phenotypes were observed.

Promoter-Reporter Lines

[0071] CRN:GUS has been previously described and characterized (Muller et al., 2008). To generate CLV2:H2B-mCherry, vector pMDC99 (Curtis and Grossniklaus, 2003) was modified by introducing the CDS of chimeric construct mCherry-H2B at the 3′ site of the gateway cassette using the unique Pad restriction site to give pAB149. To analyze the expression of CLV2 1252 bp of the 5′ region and 9 bp of the CDS was amplified using the primers AB_CLV2_Pro_F (5′ CACCAGACACAAAGCCCTTTCCATTGTC 3′; SEQ ID NO:1) and AB_CLV2_Pro_R (5′ CTTTATCATAGCTCAGAGGA 3′; SEQ ID NO:2) to give a CACC-TOPO containing amplicon, which was cloned into pENTR/D-TOPO (Invitrogen™). This entry clone was used in a LR reaction with pAB149 to give pAB183 (CLV2:H2B-mCherry). Expression of CLV2 under the control of the endogenous promoter, using 1252 bp of the CLV2 5′ region was sufficient to rescue the clv2-1 mutant in all isolated lines (N=20).

Nematode Infection of Promoter-Reporter Lines

[0072] The beet cyst nematode (BCN) Heterodera schachtii was propagated on greenhouse-grown sugar beets (Beta vulgaris cv Monohi). BCN eggs were isolated and hatched as previously described (Mitchum et al., 2004). After 2 days, second stage juveniles (J2) were collected and surfaced sterilized according to Wang et al. (2007) except 0.004% mercuric chloride, 0.004% sodium azide, and 0.002% Triton X-100 were used. Sterilized seeds were grown on modified Knop's medium (Brunschwig Chemie) (Sijmons et al., 1991). Ten days after germination seedlings were inoculated with 20 sterilized J2/root.

Histochemical β-Glucuronidase (GUS) Assays

[0073] At the indicated timepoints, freshly excised CRN:GUS tissues were infiltrated with GUS substrate buffer (0.5 mM 5-bromo-4chloro-3-indolyl glucuronide, 100 mM Tris, pH 7.0, 50 mM NaCl, 0.06% Triton X-100, 3 mM potassium ferricyanide) and incubated overnight at 37° C. (Jefferson et al., 1987). Stained roots were placed in glass Petri dishes and visualized with a Nikon Eclipse TS 100 inverted microscope.

Confocal Microscopy

[0074] CLV2:H2B-mCherry seed was sterilized, grown, and inoculated with nematodes as described above. At the indicated timepoints, infected roots were mounted on glass slides and visualized with a 510 META confocal scanning microscope (Carl Zeiss, Thornwood, N.Y., USA) excited at 543 nm.

Infection Assay with Receptor Mutants

[0075] Sterilized receptor mutants were plated in 12-well Falcon tissue culture plates (BD Biosciences) containing modified Knop's medium with 0.8% Daishin agar in a randomized block design. Plants were grown at 24° C. with a 12 hour photoperiod. Fourteen days after germination, seedlings were inoculated with 200 surface-sterilized BCN J2. J4 females were counted at 14 days post-inoculation (dpi) and adult females were counted at 30 dpi. The average values were calculated and significant differences were determined by using Student's t test (P<0.05). To measure syncytia size, receptor mutants were germinated on modified Knop's medium in vertical square plates and inoculated at 10 days after germination with 10 surface-sterilized BCN J2. At 14 dpi, syncytia that were transparent and only fed upon by only one nematode were visualized with a Nikon Eclipse TS 100 inverted microscope. Area of syncytia was measured using Adobe Photoshop CS5 and significant differences were determined by using Student's t test (P<0.05).

Results

CLV2 and CRN are Required for Nematode CLE Perception

[0076] We have previously shown that exogenously applied 12-aa peptides corresponding to the CLE motifs of the SCN (HgCLEs) and the BCN (HsCLEs) CLEs can function as plant CLE peptide mimics causing termination of the primary root meristem in a concentration dependent manner (Wang et al., 2010b). In Arabidopsis, it has been shown that the short root phenotype caused by overexpression or exogenous application of some plant CLE peptides is dependent on CLV2 signaling (Fiers et al., 2005; Miwa et al., 2008; Muller, 2008; Meng et al., 2010). More recent evidence indicates that CLV2 forms a complex with CRN and can transmit the signal from CLV3 binding in a CLV1-independent manner (Miwa et al., 2008; Muller, 2008; Bleckmann et al., 2010; Zhu et al., 2010). To determine whether or not CLV2 and CRN might play a role in cyst nematode CLE perception we screened the Arabidopsis clv2-1 null mutant and the crn-1 amorphic allele for resistance to the HgCLE, HsCLE1, and HsCLE2 12-aa peptides. Seeds were grown on vertical plates in the absence of exogenous peptide or in the presence of 1 μM HgCLE or 10 μM of the HsCLEs and roots were measured 9 days after germination. Wild-type seedlings (Landsberg erecta [Ler]) had significantly shorter roots when grown on plates with any of the CLE peptides in comparison to the no peptide control (FIG. 1a). In contrast, clv2-1 and crn-1 root growth was relatively unimpaired in the presence of the different CLE peptides (FIG. 1a). The same observation was made with sol2-1, another mutant allele of CRN (Miwa et al., 2008) (FIG. 5). Previous reports have indicated that the short root phenotype can be attributed to a decrease in the number of meristematic cells (Fiers et al., 2005). Using Nomarski optics we confirmed that clv2-1 and crn-1 were insensitive to peptide application resulting in root meristems that were indistinguishable from the no peptide control (FIG. 1b-d).
[0077] Nematode CLEs function in planta through a CLV2- and CRN-dependent pathway Overexpression of HgCLE2, HsCLE1, and HsCLE2 in wild-type Arabidopsis has been shown to cause wus-like phenotypes similar to other plant CLEs (Strabala et al., 2006; Meng et al., 2010; Wang et al., 2005; Wang et al., 2010a; Wang et al., 2010b). If CLV2 and/or CRN are involved in nematode CLE perception then we would expect the phenotypes to be diminished or abolished when overexpressed in clv2-1 and/or crn-1. Each of the nematode CLE genes was cloned into an overexpression vector and transformed into the mutant backgrounds. Transgenic seedlings in the T1 generation were screened and characterized in soil. In contrast to the overexpression phenotypes seen in wild-type Arabidopsis where a high percentage of wus-like phenotypes were observed (Wang et al., 2010a; Wang et al., 2010b), no wus [see pdf for image] -like phenotypes were observed when HgCLE2, HsCLE 1, and HsCLE2 were overexpressed in clv2-1 or crn-1 (Table 1). These results demonstrate that mutations in CRN and CLV2 suppress nematode CLE overexpression phenotypes.
[0078] 
[00001] [TABLE-US-00001]
  TABLE 1
 
  Summary of nematode CLE overexpression
  phenotypes in clv2-1 and crn-1.
    T1 Shoot Phenotypes
  Background   Construct   wus-like (%)   WT (%)   Total T1 (#)
 
  clv2-1   HgCLE2   0   100   96
    HsCLE1   0   100   67
    HsCLE2   0   100   28
  crn-1   HgCLE2   0   100   85
    HsCLE1   0   100   41
    HsCLE2   0   100   37
 
[0079] Spatial and temporal relationship between CLV2, CRN, and nematode feeding sites Cyst nematodes enter the root near the zone of elongation, migrate through root cortical cells using their stylet to puncture through cell walls, and begin feeding from a single cell near the vascular cylinder. Once cyst nematodes initiate a feeding site the dorsal esophageal gland cell becomes active and the secreted CLE peptides are delivered to the host root cells (Wang et al., 2010a). In order for CLV2 and CRN to be able to perceive the nematode CLE as a ligand mimic they must be expressed in the correct spatial and temporal context.
[0080] Using a CRN:GUS transgene in Arabidopsis, CRN expression was previously shown to be expressed throughout the root including the vasculature where the nematode initiates feeding (FIG. 2a-c; Muller et al., 2008). To confirm whether CRN is expressed in nematode feeding sites, transgenic Arabidopsis seedlings expressing CRN:GUS were infected with BCN and monitored during nematode development. GUS expression was detected in feeding sites as soon as early second-stage juveniles (J2) began to feed. (FIG. 2d). GUS expression reached its peak once nematodes reached late J2 parasitic stages, but remained detectable in the feeding sites of third stage juvenile (J3) parasitic nematodes (FIGS. 2e and f). By the time the nematodes reached the fourth stage juvenile (J4) life stage, GUS expression was either weak or absent in feeding sites (FIG. 2g).
[0081] Similar to CRN, CLV2 is expressed in many different vegetative tissues (Jeong et al., 1999). However little is known about the expression pattern of CLV2 in roots. To visualize CLV2 expression in roots and nematode feeding sites, mCherry was fused to the C-terminus of the Arabidopsis Histone 2B (H2B) gene and placed under the transcriptional control of the CLV2 promoter. The H2B protein has been shown to be a valid marker for chromatin organization in plant nuclei and has been used to describe development of the syncytial endosperm in Arabidopsis (Boisnard-Lorig et al., 2001). In uninfected roots, CLV2:H2B-mCherry fluorescence was detected throughout the root vasculature with the strongest expression detected in lateral root primordia and the zone of elongation extending down to the root apical meristem (A. Bleckmann and R. Simon, unpublished). Upon nematode infection, increased expression of CLV2:H2B-mCherry fluorescence was detected in the nuclei of syncytia fed upon by parasitic J2s (FIG. 3a-b). At the J3 life stage CLV2:H2B-mCherry continued to be specifically expressed within feeding sites (FIG. 3c-d). No fluorescence was detected in nuclei of syncytia fed upon by parasitic J2s in wild-type plants (FIG. 6a-b).
[0082] Mutant alleles of CLV2 and CRN cause a reduction in nematode infection and defects in syncytial size.
[0083] By using an RNAi approach targeting nematode CLE genes, previous reports have shown that nematode CLE peptides are important for successful infection of host plants roots (Bakhetia et al., 2007; Patel et al., 2008). To determine if nematode CLE perception by CLV2 or CRN is required, root infection assays with nematodes were performed on the clv2-1 and crn-1 single mutants, and the crn-1 clv2-1 double mutant. According to Muller et al. (2008), crn-1 clv2-1 is morphologically indistinguishable from either of the single mutants, indicating that they act in the same pathway. The mutant alleles and the wild-type Ler were randomized in 12-well plates and grown on modified Knop's medium.
[0084] Two weeks after germination seedlings were inoculated with infective J2s. J4 females were counted at 14 days post-inoculation (dpi) and adult females were counted at 30 dpi. Both the single and double mutants showed a statistically significant reduction in nematode infection with the exception of crn-1 at 14 dpi (FIG. 4a). At 30 dpi nematode infection was reduced by approximately 25% in all receptor mutants tested. A similar reduction in nematode infection across all mutant lines supports the hypothesis that CLV2 and CRN are acting in the same signaling pathway. Using sol2-1, we observed a 40% reduction in nematode infection (FIG. 7a). Since the establishment of a feeding site is required for nematode development and reproduction, the above observations motivated us to determine if there were any defects in syncytial size between the receptor mutants and wild-type.
[0085] The mutant alleles and the wild-type Ler were grown on vertical square plates and inoculated with infective J2s. At 14 dpi, syncytia that were transparent and fed upon by only one nematode were measured. The average area of wild-type (Ler) syncytia was 1402±147 μm2 (FIG. 4b). In contrast, the syncytia of the receptor mutant alleles were reduced by approximately 40%. The average area of crn-1, clv2-1, and crn-1 clv2-1 was 797±89 μm2, 745±61 μm2, and 808±57 μm2, respectively (FIG. 4b). The same reduction in syncytia size was seen in the sol2-1 mutant allele (FIG. 7b).
[0086] Nematode CLE genes have been found to be upregulated in the dorsal esophageal gland cell at the onset of parasitism and remain on through the adult female life stage. CLE genes are turned off in adult males that are no longer feeding (Wang et al., 2005; Patel et al., 2008; Lu et al., 2009; Wang et al., 2010a). In SCN and BCN, immunolocalization studies have localized nematode CLEs along the dorsal gland extension and in the ampulla at the base of the nematode stylet indicating they are secreted into host plant roots via the stylet (Wang et al., 2005; Patel et al., 2008; Wang et al., 2010a). Consistent with these results an immunofluorescence study found that SCN CLEs are secreted directly into host plant root cytoplasm (Wang et al., 2010a). The variable domain of SCN CLEs is then able to redirect the proteins into the apoplast where they can act as plant CLE ligand mimics by interacting with extracellular membrane bound plant CLE receptors. However, thus far, host plant receptors that perceive nematode CLE signals have not been identified.
[0087] Many studies have used synthetic CLE peptides to help determine the roles that plant CLE peptides play in plant growth and development. Previous studies have shown that nematode CLE peptides cause root growth phenotypes similar to other plant CLEs (Lu et al., 2009; Wang et al., 2010a; Wang et al., 2010b). Other studies have also shown that these peptide screens can identify receptors that may be involved in certain CLE signaling pathways by utilizing receptor mutants (Fiers et al., 2005; Stahl et al., 2009; Meng et al., 2010).
[0088] To identify potential nematode CLE receptors we tested plant CLE receptors implicated in CLE signaling in the RAM for a role in nematode CLE perception. In the root, exogenous peptide assays and overexpression studies have shown that CLV2 is required for proper proximal meristem function (Stahl et al., 2009; Meng et al., 2010).
[0089] It has also been shown that a new member of the receptor kinase family, CRN, forms a heterodimer with CLV2 and is required for proper localization of the CLV2/CRN complex to the plasma membrane (Bleckmann et al., 2010; Zhu et al., 2010). In Arabidopsis, CRN has been found to be widely expressed in both shoot and root tissues suggesting dual roles in shoot and root development (Muller et al., 2008). CLV2 has been found to be expressed in shoot tissues (Jeong et al., 1999), but less is known about its expression in the root. In this paper we screened a null mutant allele of CLV2 and an amorphic mutant allele of CRN for resistance to the nematode CLE peptides. Both clv2-1 and crn-1 were resistant to HgCLEp, HsCLE1p, and HsCLE2p (FIGS. 1 and 5). Similar to synthetic peptide assays, overexpression of HgCLE, HsCLE1, and HsCLE2 in the clv2-1 and crn-1 mutant backgrounds abolished the wus-like phenotypes seen when the nematode CLEs are overexpressed in wild-type backgrounds (Wang et al., 2005; Wang et al., 2010a; Wang et al., 2010b). Taken together, the peptide assays and overexpression data indicate that CLV2 and CRN are required for nematode CLE perception.
[0090] In order to serve as a receptor complex for nematode CLE peptides, CLV2 and CRN would most likely need to be expressed in feeding cell initials as well as the developing feeding sites. With the use of promoter-reporter lines we confirmed that both CLV2 and CRN were expressed in nematode-induced feeding sites (FIGS. 2 and 3), consistent with a role in nematode CLE perception. It is also possible that nematode CLE receptors are expressed in the cells adjacent to the expanding syncytium. As the nematode CLEs are redirected to the host root apoplast, extracellular receptors of the adjacent cells that are primed for incorporation could trigger plant CLE signaling pathways needed to fully form the syncytium. In the future it will be interesting to more precisely localize the CLV2 and CRN proteins within syncytia using immunofluorescence techniques. This will aid in determining whether or not these nematode CLE receptors are expressed within the cell wall openings that occur during syncytium formation or if they are expressed on the outer plasma membrane of the syncytium and/or adjacent cells.
[0091] Previous reports have demonstrated that SCN and BCN CLEs are important for nematode parasitism by showing a reduction in nematode infection after knocking down CLE expression in the worm using RNAi approaches (Bakhetia et al., 2007; Patel et al., 2008). To directly test for a role of CLV2/CRN in nematode CLE perception we performed infection assays on the receptor mutants.
[0092] We showed that a reduction in nematode infection occurs on the receptor mutants (FIGS. 4a and 6). Concurrently, we also saw a reduction in syncytium size in the receptor mutants (FIGS. 4b and 7). The fact that we saw a similar reduction in both nematode infection and syncytia size in both the single and double mutants is consistent with genetic and biochemical data that CLV2 and CRN are acting in the same pathway (Muller et al., 2008; Bleckmann et al., 2010; Zhu et al., 2010). These data indicate that not only is nematode CLE perception by CLV2 and CRN important for successful nematode infection, but demonstrates that CLE signaling also plays a role feeding cell formation.
[0093] The involvement of CRN in nematode CLE signaling also opens up the interesting possibility that nematode CLE signaling may be directly or indirectly suppressing host plant defense responses. It has been reported that in root tips of sol2-1, another mutant allele of CRN, plant disease resistance-related and stress responsive genes were upregulated (Miwa et al., 2008). Therefore, when nematode CLEs are secreted they could activate the CLV2/CRN signaling pathway leading to a suppression of plant disease resistance-related and plant stress responsive genes. One might speculate that the main target for nematode CLEs is a signaling pathway which allows developmental programming of root cells for syncytium formation to occur and that suppression of plant defense responses is just an added benefit to the nematode. Alternatively, the nematode may require suppression of plant defense responses through plant CLE signaling in order for the syncytium to form properly. Further studies will need to be performed to investigate this possibility.
[0094] Several possibilities exist for why we only see a partial reduction in nematode numbers and syncytia size in the clv2-1 and crn-1 mutant backgrounds. First, besides CLEs, nematodes secrete many different effectors that likely play an important role in feeding cell formation (Wang et al., 2001; Gao et al., 2003).
[0095] For example, when BCN CLEs were targeted with RNAi a similar partial reduction in nematode infection was observed (Patel et al., 2008), either as a consequence of limited reductions in transcript levels or an indication that the other effectors still active in the nematode allow infection to proceed. A second possibility for the partial reduction in the receptor mutants is that there could be multiple nematode CLE receptors. So far, the nematode CLEs reported belong to gene families (Lu et al., 2009; Wang et al., 2010a; Wang et al., 2010b). In addition, PCN CLEs have multiple CLE motifs that may be simultaneously processed to release different CLE peptides (Lu et al., 2009). This leaves the possibility that nematode CLE peptides may activate multiple plant CLE signaling pathways concurrently to function in an antagonistic or synergistic fashion as reported for plant CLEs (Whitford et al., 2008). The current plant CLV3 signaling pathway in the shoot indicates that there are parallel signaling pathways. Genetic evidence indicates that CLV1 acts in a separate pathway from the CLV2/CRN pathway (Muller et al., 2008). In support of the genetic data, recent reports using luciferase complementation assays and FRET analysis have shown that CLV1 forms a homodimer and that CLV2 and CRN form a heterodimer without CLV3 stimulation (Bleckmann et al., 2010; Zhu et al., 2010).
[0096] These reports also found evidence for CLV1 interacting with the CLV2/CRN complex leading to the possibility that different signaling pathways could be activated depending on which receptor in the complex interacts with the CLE ligand (Bleckmann et al., 2010; Zhu et al., 2010). Thus it is possible that in the crn-1 clv2-1 double mutants, nematodes are still able to signal through other receptors in the roots. Unlike CLV2, which has a broad expression pattern in plants, CLV1 expression is thought to be restricted to the center of the SAM and its function is thought to be confined to stem cell specification in the shoot (Clark et al., 1997; Fletcher et al., 1999). Therefore, in order to utilize CLV1 as a receptor, nematodes would have to activate CLV1 expression in the roots. Recently, CLV1-related Barely Any Meristem (BAM) 1 and BAM2 have been shown to act redundantly in the SAM and are widely expressed throughout the plant, including root tissues (DeYoung et al., 2006; Deyoung and Clark, 2008). We have found that bam1 is also resistant to exogenous application of synthetic nematode CLE peptides (A. Replogle, S. Chen, X. Wang and M. G. Mitchum, unpublished data). Moreover, there are over 200 LRR-RLKs in Arabidopsis and only a few receptor-CLE ligand pairs have been identified (Shiu and Bleecker, 2001). Thus, further studies using a combination of mutants will need to be performed to investigate the possible involvement of other host plant receptors in nematode CLE signaling.
[0097] It is shown here that nematode CLE signaling through the CLV2/CRN receptor complex is important for proper syncytium formation and ultimately successful nematode infection. These findings open the door for identifying the downstream signaling components regulated by CLV2/CRN to uncover the role nematode CLE signaling plays in syncytium formation.

References

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[0106] Davis, E. L., Hussey, R. S, and Baum, T. J. (2004) Getting to the roots of parasitism by nematodes. Trends Parasitol. 20, 134-141.
[0107] Davis, E. L., Hussey, R. S., Mitchum, M. G. and Baum, T. J. (2008) Parasitism proteins in nematode-plant interactions. Curr. Opin. Plant Biol. 11, 360-366.
[0108] DeYoung, B. J., Bickle, K. L., Schrage, K. J., Muskett, P., Patel, K. and Clark, S. E. (2006) The CLAVATA1-related BAM1, BAM2 and BAM3 receptor kinase-like proteins are required for meristem function in Arabidopsis. Plant J. 45, 1-16.
[0109] Deyoung, B. J. and Clark, S. E. (2008) BAM receptors regulate stem cell specification and organ development through complex interactions with CLAVATA signaling. Genetics, 180, 895-904.
[0110] Endo, B. Y. (1964) Penetration and development of Heterodera glycines in soybean roots and related anatomical changes. Phytopathology, 54, 79-88.
[0111] Fiers, M., Hause, G., Boutilier, K., Casamitjana-Martinez, E., Weijers, D., Offring a, R., van der Geest, L., van Lookeren Campagne, M. and Liu, C. M. (2004) Mis-expression of the CLV3/ESR-like gene CLE19 in Arabidopsis leads to a consumption of root meristem. Gene, 327, 37-49.
[0112] Fiers, M., Golemiec, E., Xu, J., van der Geest, L., Heidstra, R., Stiekema, W. and Liu, C. M. (2005) The 14-amino acid CLV3, CLE19, and CLE40 peptides trigger consumption of the root meristem in Arabidopsis through a CLAVATA2-dependent pathway. Plant Cell, 17, 2542-2553.
[0113] Fletcher, L. C., Brand, U., Running, M. P., Simon, R. and Meyerowitz, E. M. (1999) Signaling of cell fate decisions by CLAVATA3 in Arabidopsis shoot meristems. Science, 283, 1911-1914.
[0114] Gao, B., Allen, R., Maier, T., Davis, E. L., Baum, T. J. and Hussey, R. S. (2003) The parasitome of the phytonematode Heterodera glycines. Mol. Plant Microbe Interact. 16, 720-726.
[0115] Jefferson, R. A., Kavanagh, T. A. and Bevan, M. W. (1987) GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J, 6, 3901-3907.
[0116] Jeong, S., Trotochaud, A. E. and Clark, S. E. (1999) The Arabidopsis CLAVATA2 gene encodes a receptor-like protein required for the stability of the CLAVATA1 receptor-like kinase. Plant Cell, 11, 1925-1934.
[0117] Kayes, J. M. and Clark, S. E. (1998) CLAVATA2, a regulator of meristem and organ development in Arabidopsis. Development, 125, 3843-3851.
[0118] Koornneef, M., Van Eden, J., Hanhart, C. J., Stam, P., Braaksma, F. J. and Feenstra, W. J. (1983) Linkage Map of Arabidopsis thaliana. J. Hered. 74, 265-272.
[0119] Laux, T., Mayer, K. F. X., Berger, J. and Jurgens, G. (1996) The WUSCHEL gene is required for shoot and floral meristem integrity in Arabidopsis. Development, 122, 87-96.
[0120] Lu, S. W., Chen, S., Wang, J., Yu, H., Chronis, D., Mitchum, M. G. and Wang, X. (2009) Structural and functional diversity of CLAVATA3/ESR (CLE)-like genes from the potato cyst nematode Globodera rostochiensis. Mol. Plant Microbe Interact. 22, 1128-1142.
[0121] Meng, L., Ruth, K. C., Fletcher, J. C. and Feldman, L. (2010) The roles of different CLE domains in Arabidopsis CLE polypeptide activity and functional specificity. Mol. Plant, doi:10.1093/mp/ssq021
[0122] Mitchum, M. G., Sukno, S., Wang, X., Shani, Z., Tsabary, G., Shoseyov, O. and Davis, E. L. (2004) The promoter of the Arabidopsis thaliana Cell endo-1,4-beta glucanase gene is differentially expressed in plant feeding cells induced by root-knot and cyst nematodes. Mol. Plant Pathol. 5, 175-181.
[0123] Mitchum, M. G., Wang, X. H. and Davis, E. L. (2008) Diverse and conserved roles of CLE peptides. Curr. Opin in Plant Biol. 11, 75-81.
[0124] Miwa, H., Betsuyaku, S., Iwamoto, K., Kinoshita, A., Fukuda, H. and Sawa, S. (2008) The receptor-like kinase SOL2 mediates CLE signaling in Arabidopsis. Plant Cell Physiol. 49, 1752-1757.
[0125] Muller, R., Bleckmann, A. and Simon, R. (2008) The receptor kinase CORYNE of Arabidopsis transmits the stem cell-limiting signal CLAVATA3 independently of CLAVATA1. Plant Cell, 20, 934-946.
[0126] Ogawa, M., Shinohara, H., Sakagami, Y. and Matsubayashi, Y. (2008) Arabidopsis CLV3 peptide directly binds CLV1 ectodomain. Science, 319, 294.
[0127] Patel, N., Hamamouch, N., Chunying, L., Hussey, R., Mitchum, M., Baum, T., Wang, X. and Davis, E. L. (2008) Similarity and functional analyses of expressed parasitism genes in Heterodera schachtii and Heterodera glycines. J. Nematol. 40, 299-310.
[0128] Rojo, E., Sharma, V. K., Kovaleva, V., Raikhel, N. V. and Fletcher, J. C. (2002) CLV3 is localized to the extracellular space, where it activates the Arabidopsis CLAVATA stem cell signaling pathway. Plant Cell, 14, 969-977.
[0129] Sarkar, A. K., Luijten, M., Miyashima, S., Lenhard, M., Hashimoto, T., Nakajima, K., Scheres, B., Heidstra, R. and Laux, T. (2007) Conserved factors regulate signaling in Arabidopsis thaliana shoot and root stem cell organizers. Nature, 446, 811-814.
[0130] Sasser, J. N. and Freckman, D. W. (1987) A world perspective on Nematology: the role of the society. Vistas on nematology. Veech, J. A. and Dickson, D. W., Hyatssville, Md., USA: Society of Nematologists.
[0131] Sharma, V. K., Ramirez, J. and Fletcher, J. C. (2003) The Arabidopsis CLV3-like (CLE) genes are expressed in diverse tissues and encode secreted proteins. Plant Mol. Biol. 51, 415-425.
[0132] Shiu, S. H. and Bleecker, A. B. (2001) Plant receptor-like kinase gene family: diversity, function, and signaling. Sci STKE, 2001, re22.
[0133] Sijmons, P. C., Grundler, F. M. W., Von Mende, N., Burrows, P. R. and Wyss, U. (1991) Arabidopsis thaliana as a new model host for plant parasitic nematodes. Plant J. 1, 245-254.
[0134] Simon, R. and Stahl, T. (2006) Plant Cells CLEave Their Way to Differentiation. Science, 313, 773-774.
[0135] Stahl, Y., Wink, R. H., Ingram, G. C. and Simon, R. (2009) A signaling module controlling the stem cell niche in Arabidopsis root meristems. Curr. Biol. 19, 909-914.
[0136] Strabala, T. J., O'Donnell, P. J., Smit, A. M., Ampomah-Dwamena, C., Martin, E. J., Netzler, N., Nieuwenhuizen, N. J., Quinn, B. D., Foote, H. C. C. and Hudson, K. R. (2006) Gain-of-function phenotypes of many CLAVATA3/ESR genes, including four new family members, correlate with tandem variations in the conserved CLAVATA3/ESR domain. Plant Physiol. 140, 1331-1344.
[0137] Trotochaud, A. E., Hao, T., Wu, G., Yang, Z. and Clark, S. E. (1999) The CLAVATA1 receptor-like kinase requires CLAVATA3 for its assembly into a signaling complex that includes KAPP and a Rho-related protein. Plant Cell, 11, 393-406.
[0138] Wang, J., Lee, C., Replogle, A., Joshi, S., Korkin, D., Hussey, R., Baum, T. J., Davis, E. L., Wang, X. and Mitchum, M. G. (2010a) Dual roles for the variable domain in protein trafficking and host-specific recognition of Heterodera glycines CLE effector proteins. New Phytol. 10.1111/j.1469-8137.2010.03300.x
[0139] Wang, J., Replogle, A., Hussey, R., Baum, T., Wang, X., Davis, E. L. and Mitchum, M. G. (2010b) Identification of potential host plant mimics of CLV3/ESR (CLE)-like peptides from the plant-parasitic nematode Heterodera schachtii. Mol. Plant Pathol. (under review).
[0140] Wang, X., Allen, R., Ding, X., Goellner, M., Maier, T., de Boer, J. M., Baum, T. J., Hussey, R. S. and Davis, E. L. (2001) Signal peptide-selection of cDNA cloned directly from the esophageal gland cells of the soybean cyst nematode Heterodera glycines. Mol. Plant Microbe Interact. 14, 536-544.
[0141] Wang, X., Mitchum, M. G., Gao, B., Li, C., Diab, H., Baum, T. J., Hussey, R. S. and Davis, E. L. (2005) A parasitism gene from a plant-parasitic nematode with function similar to CLAVATA3/ESR (CLE) of Arabidopsis thaliana. Mol. Plant Pathol. 6, 187-191.
[0142] Wang, X., Replogle, A., Davis, E. L. and Mitchum, M. G. (2007) The tobacco Cel7 gene promoter is auxin-responsive and locally induced in nematode feeding sites of heterologous plants. Mol. Plant Pathol. 8, 423-436.
[0143] Whitford, R., Fernandez, A., De Groodt, R., Ortega, E. and Hilson, P. (2008) Plant CLE peptides from two distinct functional classes synergistically induce division of vascular cells. Proc. Natl. Acad. Sci. USA, 105, 18625-18630.
[0144] Zhu, Y., Wang, Y., Li, R., Song, X., Wang, Q., Huang, S., Jin, J. B., Liu, C. M. and Lin, J. (2010) Analysis of interactions among the CLAVATA3 receptors reveals a direct interaction between CLAVATA2 and CORYNE in Arabidopsis. Plant J. 61, 223-233.

Example 2

[0145] Screening of plant CLE receptor mutants for resistance to nematode CLE peptides, overexpression of the nematode CLEs in the receptor mutant background, and infection assays of plant receptor mutants, has identified several receptors involved in nematode CLE peptide signaling. Plant receptor mutants exhibiting resistance to exogenous treatment of nematode CLE peptides include CLAVATA2 (CLV2; At1g65380), CORYNE (CRN; At5g13290), BARELY ANY MERISTEM (BAM1; At5g65700), and ERECTA-LIKE2 (ERL2; (At5g07180) (FIGS. 8 and 9). Overexpression of nematode CLEs in the clv2 and crn mutant background abolished all phenotypes (Table 2) that are observed when nematode CLEs are overexpressed in wild type plants (Wang et al., 2005; 2010: Lu et al., 2009). Additionally, nematode infection is significantly reduced on several of the receptor mutants including clv1, clv2, and crn (FIG. 10). Expression of receptors in nematode feeding cells was confirmed by infection of transgenic plants containing promoter-reporter fusions (FIGS. 11-13) and upregulation of candidate soybean and potato receptor genes in H. glycines-induced syncytia and G. rostochiensis-infected potato roots were revealed by microarray analysis of laser-captured syncytia (Table 2, 5% FDR; Ithal et al., 2007) and qRT-PCR analysis (FIG. 14). Thus, the disruption or modulation of the host plant receptor proteins that perceive the nematode CLE peptides can be used to develop a novel management tactic to reduce cyst nematode parasitism in crop plants including, but not limited to potato or soybean. Nematode control can thus be obtained by inhibiting receptor proteins that interact with any nematode CLE peptide. Endogenous plant gene encoding a receptor for a nematode CLE peptide that can be inhibited to provide include functional or structural orthologs of the receptor proteins in any plant species, including but not limited to receptor genes from monocot or dicot plant, or receptor genes selected from the group consisting of a tobacco, cereal, sugar beet, cotton, fruit, fiber, oilseed, potato, rice, corn, soybean, vegetable, and wheat plant.
[0146] 
[00002] [TABLE-US-00002]
    TABLE 2
   
    T1 Shoot Phenotypes
  Background   Construct   wus (%)   PNC (%)   WT (%)   Total (#)
 
  Col-0HgCLE2ΔSP   78   6   15   80
    HsCLE1   30   7   63   156
    HsCLE2   6   5   89   158
  clv2-1HgCLE2ΔSP   0   0   100   96
    HsCLE1   0   0   100   67
    HsCLE2   0   0   100   28
  crn-1HgCLE2ΔSP   0   0   100   85
    HsCLE1   0   0   100   41
    HsCLE2   0   0   100   37
 
[0147] 
[00003] [TABLE-US-00003]
  TABLE 3
 
  Putative Soybean Receptors Upregulated in Soybean Cyst Nematode
  Induced Syncytia (5% FDR, LCM W82)
  Sequences corresponding to the genes provided below can be obtained from the world wide
  web (internet) using the identifiers provided in Table 3 from the following internet locations:
  1) “the world wide web at soybase.org” or the world wide web at soybase.org/gbrowse/cgi-bin/gbrowse/gmax1.01/
  2) “the world wide web at phytozome.net” or the world wide web at phytozome.net/cgi-bin/gbrowse/soybean/?name=Gm09
  3) “the world wide web at plantgdb.org” or the world wide web at plantgdb.org/GmGDB/(Assembly version Glyrna1.170
  (April 2009)
  4) the world wide web at ncbi.nlm.nih.gov/sites/entrez
  Affymetrix Probset   Qvalue   FC dpl 2   Soybean Gene   Best At Hit   E-value   PFAM Description(30)
 
  Putative Soybean Leucine-Rich Repeat Receptor-Like Kinase (LRR-RLK)
  Upregulated
  Gma.1778.1.S1_at   0.000696   11.19428   Glyma08g16220.1   AT3G12610.1   1E−100   Leucine Rich Repeat
  GmaAffx.78459.1.S1_at   0.000947   15.6836   Glyma18g42700.1   AT4G08850.1   0   Leucine rich repeat N-terminal domain
  Gma.17727.3.A1_at   0.001475   1.696212   Glyma13g37580.1   AT4G03390.1   0   Leucine rich repeat N-terminal domain
  Gma.9956.1.S1_at   0.001606   5.057105   Glyma15g26790.1   AT5G06860.1   1E−100   Leucine rich repeat N-terminal domain
  GmaAffx.3809.1_S1_at   0.001904   1.789864   Glyma18g00610.1   AT3G23750.1   0   Leucine rich repeat N-terminal domain
  GmaAffx.50347.1.S1_at   0.002786   26.80867   Glyma16g06940.1   AT4G08850.1   0   Leucine rich repeat N-terminal domain
  GmaAffx.91749.1.S1_s_at   0.003961   12.73998   Glyma15g26790.1   AT5G06860.1   1E−100   Leucine rich repeat N-terminal domain
  GmaAffx.49400.1.S1_at   0.006842   2.043868   Glyma07g31970.1   AT1G28340.1   0   Leucine rich repeat N-terminal domain
  Gma.17727.1.A1_at   0.007366   2.014757   Glyma13g37580.1   AT4G03390.1   0   Leucine rich repeat N-terminal domain
  GmaAffx.47891.1.S1_s_at   0.007905   8.874029   Glyma19g32700.1   AT5G06860.1   1E−112   Leucine rich repeat N-terminal domain
  GmaAffx.68769.1.S1_at   0.008658   4.20926   Glyma20g25570.1   AT2G01210.1   0   Leucine rich repeat N-terminal domain
  GmaAffx.24529.1.S1_at   0.008905   32.2986   Glyma12g00960.1   AT4G08850.1   0   Leucine rich repeat N-terminal domain
  GmaAffx.5435.1.A1_at   0.009418   1.368383   Glyma09g13540.1   AT5G51350.1   0   Leucine rich repeat N-terminal domain
  GmaAffx.65770.1.S1_at   0.012282   1.967988   Glyma18g06670.1   AT3G56050.2   1E−115   Leucine Rich Repeat
  Gma.9483.1.A1_at   0.016684   2.107688   Glyma19g29370.1   AT3G03770.1   0   Leucine Rich Repeat
  Gma.16818.1.S1_s_at   0.017963   2.874609   Glyma11g04700.1   AT5G65700.1   0   Leucine rich repeat N-terminal domain
          (BAM1)    
  GmaAffx.6107.2.A1_at   0.018015   14.17985   Glyma06g13970.1   AT3G47570.1   0   Leucine rich repeat N-terminal domain
  GmaAffx.51790.1.S1_at   0.023845   3.00325   Glyma17g18520.1   AT5G67200.1   0   Leucine rich repeat N-terminal domain
  Gma.405.1.A1_at   0.026584   16.80313   Glyma11g13970.1   AT3G20820.1   1E−125   Leucine rich repeat N-terminal domain
  GmaAffx.24583.1.S1_at   0.02776   1.951831   Glyma05g29150.1   AT4G18640.1   1E−169   Leucine rich repeat N-terminal domain
  Gma.16642.1.S1_at   0.034358   1.219904   Glyma12g03370.1   AT1G10850.1   0   Leucine rich repeat N-terminal domain
  Gma.4557.1.S1_at   0.040772   1.199444   Glyma08g01640.1   AT1G63430.1   0   Leucine Rich Repeat
  Gma.5232.1.A1_at   0.044053   1.576101   Glyma06g36230.1   AT5G53890.1   0   Leucine rich repeat N-terminal domain
  GmaAffx.18359.1.S1_at   0.047925   4.386757   Glyma03g32270.1   AT1G35710.1   0   Leucine rich repeat N-terminal domain
  Gma.2806.1.S1_at   0.047969   1.0518   Glyma05g29530.1   AT1G29750.2   0   Leucine Rich Repeat
  Gma.15907.1.A1_at   0.048874   1.454241   Glyma18g53970.1   AT2G33490.1   1E−164   Leucine rich repeat N-terminal domain
  Other Putative Soybean Receptor-Like Kinases
  Upregulated
  Gma.17579.1.S1_at   0.001029   2.926118   Glyma06g07170.1   AT4G32300.1   0   D-mannose binding lectin|Protein kinase
  Gma.10903.1.A1_at   0.001984   3.921658   Glyma06g06620.1   AT5G54590.2   1E−101   Protein kinase domain
  GmaAffx.15130.1.A1_at   0.002275   8.229938   Glyma20g27740.1   AT4G05200.1   0   DUF26|Protein kinase
  GmaAffx.46387.1.S1_at   0.002957   1.854171   Glyma18g12830.1   AT3G59110.1   0   Protein kinase domain
  Gma.11892.1.S1_at   0.003085   2.491472   Glyma18g47170.1   AT1G01540.2   0   Protein kinase domain
  Gma.13100.2.A1_at   0.003827   2.576388   Glyma16g03650.1   AT1G01540.2   0   Protein kinase domain
  GmaAffx.4463.1.A1_at   0.004197   50.44552   Glyma10g39910.1   AT4G38830.1   0   DUF26|Protein kinase
  GmaAffx.8689.1.S1_at   0.004457   6.746447   Glyma06g02000.1   AT1G20650.1   1E−134   Protein kinase domain
  Gma.5637.1.S1_at   0.005376   3.595321   Glyma10g28610.1   AT2G47180.1   1E−150   Glycosyl transferase family 8
  GmaAffx.37615.1.S1_at   0.006409   3.634731   Glyma17g06430.1   AT2G17220.2   1E−117   Protein kinase domain
  Gma.5416.1.S1_at   0.00667   1.151061   Glyma09g40650.1   AT5G01020.1   0   Protein kinase domain
  GmaAffx.65711.1.S1_at   0.007597   3.149387   Glyma18g45000.1   AT3G08600.1   5E−87   Protein of unknown function (DUF1191)
  Gma.8159.1.S1_at   0.007785   2.089075   Glyma14g03290.1   AT3G59110.1   0   Protein kinase domain
  GmaAffx.83551.1.S1_at   0.009487   36.43414   Glyma06g41150.1   AT4G21380.1   0   D-mannose binding lectin|Protein kinase
  GmaAffx.22464.1.A1_at   0.010743   2.290479   Glyma12g16650.1   AT5G54590.2   1E−176   Protein tyrosine kinase
  GmaAffx.56567.2.A1_at   0.010919   8.11891   Glyma10g05990.1   AT1G16670.1   6E−90   Protein kinase domain
  GmaAffx.60687.1.S1_at   0.014387   18.28579   Glyma08g46630.1   AT1G11300.1   1E−140   D-mannose binding lectin|PAN-like domain
  GmaAffx.8293.1.S1_at   0.015011   3.375224   Glyma08g22770.1   AT3G15890.1   1E−125   Protein kinase domain
  GmaAffx.76163.1.S1_at   0.0178   1.444014   Glyma09g32390.1   AT3G24550.1   0   Protein tyrosine kinase
  GmaAffx.87698.1.S1_at   0.020806   2.437942   Glyma12g11840.1   AT4G03390.1   1E−172   Protein tyrosine kinase
  GmaAffx.91247.1.S1_at   0.024984   2.593136   Glyma15g09490.1   AT4G18950.1   0   Ankyrin repeat|Protein kinase domain
  GmaAffx.10590.1.S1_s_at   0.026872   4.633997   Glyma06g40480.1   AT4G27290.1   0   D-mannose binding lectin|Protein kinase
  Gma.4931.1.S1_s_at   0.030153   1.894182   Glyma18g44830.1   AT3G51550.1   0   Protein tyrosine kinase
  Gma.7158.1.A1_at   0.032121   2.64156   Glyma12g08210.1   AT2G28250.2   1E−165   Protein kinase domain
  Gma.14610.1.A1_at   0.039013   4.911538   Glyma20g37470.1   AT3G05140.1   1E−153   Protein kinase domain
  Gma.3334.1.S1_at   0.042026   2.355478   Glyma02g43710.1   AT2G33580.1   1E−179   LysM domain|Protein kinase domain
  GmaAffx.93403.1.S1_at   0.042465   3.481746   Glyma05g25370.1   AT5G06870.1   7E−77   Leucine rich repeat N-terminal domain
  Gma.8195.2.S1_a_at   0.042848   2.222809   Glyma18g16060.1   AT2G02800.2   1E−171   Protein kinase domain
  GmaAffx.4480.1.S1_at   0.042861   6.312541   Glyma18g06610.1   AT5G58520.1   0   Protein tyrosine kinase
  Gma.8195.2.S1_at   0.045596   11.62797   Glyma18g16060.1   AT2G02800.2   1E−171   Protein kinase domain
  GmaAffx.19954.2.S1_at   0.045643   4.577217   Glyma10g43060.1   AT4G38470.1   0   ACT domain|Protein tyrosine kinase
 
  REFERENCES
Gao, B. L., Allen, R., Maler, T., Davis, E. L., Baum, T. J., and Hussey, R. S. (2003) The parasitome of the phytonematode Heterodera glycines. Mol. Plant-Microbe Interact, 16, 720-726.
Ithal N, Recknor J. Nettleton D, Maier T, Baum TJ, Mitchum MG. (2007) Developmental transcript profiling of cyst nematode feeding cells in soybean roots. Mol. Plant-Microbe Interact, 20(5): 510-525.
Lu, S. W., Chen, S., Wang, J., Yu, H., Chronis, D., Mitchum, M. G., and Wang, X. (2009) Structural and functional diversity of CLAVATA3/ESR (CLE)-like genes from the potato cyst nematode Globodera rostochiensis. Mol. Plant-Microbe Interact, 22, 1128-1142.
Mitchum, M. G., Wang, X. and Davis, E. L. (2008) Diverse and conserved roles of CLE peptides. Curr Opin Plant Biol, 11, 75-81.
Patel, N., Hamamouch, N., Chunying, L., Hussey, R., Mitchum, M., Baum, T., Wang, X., and Davis, E. L. (2008) Similarity and functional analyses of expressed parasitism genes in Heterodera schachtii and Heterodera glycines. J Nematol, 40, 299-310.
Wang, J., Lee, C., Replogle, A, Joshi, S., Korkin, D., Hussey, R. S., Baum, T. J., Davis, E. L., Wang, X., and Mitchum, M. G. (2010) Dual roles for the variable domain in protein trafficking and host-specific recognition of Heterodera glycines CLE effector proteins. New Phytol, dol: 10.1111/j.1469-8137.2010.03300.x
Wang, J., Replogle, A., Hussey, R. S., Baum, T. J., Wang, X., Davis, E. L., and Mitchum, M. G. (2010) Identification of potential host plant mimics of CLV3/ESR (CLE)-like peptides from the plant-parasitic nematode Heterodera schachtil, Mol. Plant Pathol. (submitted).
Wang, X. H., Allen, R., Ding, X. F., Goeliner, M., Maier, T., de Boer, J. M., Baum, T. J., Hussey, R. S., Davis, E. L. (2001) Signal peptide-selection of cDNA cloned directly from the esophageal gland cells of the soybean cyst nematode Heterodera glycines. Mol Plant-Microbe Interact, 14, 536-544.

Example 3

Promoter Sequences Useful in the Practice of the Invention

[0148] 
[00004] [TABLE-US-00004]
  BARELY ANY MERISTEM (BAM1; At5g65700) - promoter sequence;  
  SEQ ID NO: 3  
  tctcattaagcacctacttcccacatctttcttaaagtttcttacataaagctcccttcacacgtgcttaccaaatc  
 
  agattgtcaataattcttgctcaataatttttcgaaatttatttgaatttatctaataaaaatacattgtttgagta
 
  tgatattttgcttaagaaggttgattattctccctatcaaagtctaaaaagaagattacaaaacaattgtatggtta
 
  aattcatataaatttgtgactagtattttaatatttacatatatacaaatacttatagatgaaacgagaatgcagaa
 
  atgattatagatagatcagtgacagtgaactgtagcaaccggcaaagaaacctcgttagctggacacacgattacga
 
  tcatgcccccagtctcctctgtccagacggctgcattaataacaacgagctagagggtgttttcgtcttttcgatac
 
  ttatcccaaaaccgacaatctctggtttggactcgaaggctgatttggtcaattcatagcaaccgaacgagcagtcc
 
  attcaagtccaaagagctccttagtggtaaaagatgtaattacgtagatgttccatggtcaagaatgtattcagtca
 
  aaataaatatttgaccaaaactttcggttaatttcctaccaccagcaaaattataactttttctaataattatcaat
 
  cattttcaatctcttttaattttctttttcacttttttttattaattaaagtcaattcacactatacaaaaagaagg
 
  aagtctaaatatttttttactttcatgttgcttttctaacttttatattttgctcttctcaacagattttgctggtt
 
  tttgtattagaaatattattatgtttccagaaatgaattttttatatgtcgtctggattcgtatatatatattggaa
 
  agtgaaattaattcatttgatttttttctttgatatatcgaccaaatcaaataaatacgaccccattgtggcattgt
 
  taatgcaaaaaggcacaagtacaaaaaaaacataataattcactattttatttacagacacatgggcccaattcata
 
  cggcccaattaccataaacctctcttttaaagagtgggttccacagtggtaaactttttgactatccattggaatga
 
  ttgcatctggaccgttcatctacattaattattgggttttttcgctttaaagcatcaattaacttattacgtatagg
 
  attagattaccaataacgatctttttagcttttgtcgttttccgataaaaccatacgattaagaatatgacctcttg
 
  tatcttttgagggattttagttaatctttctacatttattttgttggatgctcatacaattatcctgtgtctctcaa
 
  aataaaacaaaaattactctatttattagtacattacacatgattatttagaaaatgtatattgtggtcatatgaaa
 
  tgagaaattaaaggaaatttgtcaatacttgagaacatcaccattcaaatgtttcaagaacaacatgactccaaaac
 
  aaaataaatgaacctttccctaataatagtatattctccatcgtacaaagttctaaataatacaatattcatttcgt
 
  caaagcatatgatgtgttggaatcagaattatctgcaaatgtttgaatttcaaatgttagtatcaggctatttttac
 
  tgttttatcaaatatcgtttcttctgcaatctatcacttgattgttttatcaaatcagcactagtattattgatttt
 
  gtaatttgtgtttgtctacctccaattactttttagtgttatgattagtaatgtaataaaatcacaaatctgacgtg
 
  gcacctatatacaattccaaaaacaagtggaacgaatataaaacaaattcacaccttcctcatcttcttcttcgtct
 
  tcacttaccttctctctacactcacaccatctcacaaccctaatctctcccacacaagagagatagagagaaaca
 
  CLAVATA2 (CLV2; At1g65380) - promoter sequence;
  SEQ ID NO: 4  
  CACATACATagacacaaagccctttccattgtcctcttcgtttccttttgggtaaacaaccaatctcctgatttt  
 
  tacaaaaaaggcaacatttcttagttatatatgcttgtagtgaagaaagatgtgaaagtctgacaagagaacaag
 
  acgaaggaggagtctttctccaagtcttcaacattgcagaatctgatgcatatgaacccattttctctacaaaat
 
  gttgcaaccctagagagcaaaacaaaacatacccataatcagaaatgatctgacgaaaatcgagttacaatacac
 
  aagagaacattttttttagaattctcagatattaaaaatgacacagaaagctttatgctttttcctettaaaaga
 
  ctaaacaagttgaaatctagagaaagaactgaccaacctgagacaacgagagagacttgagagatttcttcggca
 
  cttactattagatctagggtttagataccatttatatagagaaagttttagagttgcacaaaacataaattaatg
 
  tgttagaatgggcctaaagctacaaagctggcctggttttgttttaaattgttggtttcatggacattttcgaca
 
  tcttcgaacatgttattttttgagactatgcaaacttgaagctctttactcgagttgaaatcgtatgacttatag
 
  tgaaattgtacatttggtttcgatttttcttttacactctttcttctttgagccggtaaatttggaatttttctt
 
  catagtggaatcatatgctgttttttttttttatagtaaacgttacaagaatgaatggtaactttatccaaaaaa
 
  aaagaatcatattattttgaaatgattttaagtaaattctaggttcaataacataagatttgagactaaatttaa
 
  aatttcttagtaaaatatatgatttttttataaatacctataaaattagtaattaacaatacggattacgtactg
 
  aatcaaaccctttgtattttgtttttcctagaaataagtgtagatttttggaattttgcattaattaatcacttc
 
  ttgggtctgaaaggctaaaacaaaaggaaccgaaagagaatgttctctctgtctttatcttccacttccacttcc
 
  aggtcgcgttgcttcactctccattgcaaagagaggtctctgcgatttctgcaactcacccctgaaaccttctta
 
  atttacttcaactgccgctatacctaaaaacttcatctttctcctctgagctATGATAAAG
 
  CORYNE (CRN; At5g13290) - promoter sequence;
  SEQ ID NO: 5  
  aaagatgcataggcttgcggacataaaaattccggagctatg  
 
  tttcatcgttgctttcacggtctgaagagccaatcaacactaaagaaggacctctaatgg
 
  tctctagcaagtttagcccccaattaagtattgtattgatgtttttgtgatggatggata
 
  taggctgcatattgggaaattatagtgtattgtattgtgtcgtgttgtgtgtatgtggga
 
  ctatagcatcctgagtttgtcatgtccagacgttgtaacttgtaagcaattacttatggt
 
  tttgttcacttcgtattaacgtatttaatttgtggctcgattttggttttgaatctgtgt
 
  caaaactaagataatttacgtgttaaaccaggcccaagtttgaaagttaattgtcaattt
 
  tcagaccagagtacatattggtccacttattcccattacattcatagttttgagtctttt
 
  gataatagtgttaccatttcaattaggctaatcttttttcaacccaagatatttttataa
 
  aaaggaatgtggttcaaatcggaaaacaagacctaactttgaataaaagcactacagcat
 
  aaagcttttacctttaacaaaaaaaatataataattttttacaaggaaaaagaagagaaa
 
  gcaattattctcagacaaacaaaggaaccacttttgtaggtgtagtagtaatctcacacg
 
  ctaagacaaaagtgcacaaattctcgagactctcttctatccaacggtccatatctcact
 
  aaccgcatctaaataacggacaagatcttcttttggcttcagctctctttagtctttacc
 
  ttccctcaagctcggtactcgatgtcttgctttcggccactcatgaaagcaacgagagct
 
  tcccctttcatccgcctacgtggctatgggacccagtctaaccacgaccacctgacatcg
 
  tgggccccactgtaaggcgggaaccccatttttttttggctgtaagtaacggattctcgg
 
  tcatgcttttttgtgaggatagagagagagactgagagagagagagagtgtgtcacggtc
 
  tcgcagatactgtgtattgaaaagagagttctagagagagagtgtgttatgtgtgtgtgt
 
  gtgtgtgtgtgtgtgtgtgtgtgtgtttggttactgggattaattgagctgaaacagttt
 
  ggatagttttgtttgttctgtttcatctttcaaccacagatatagtaatattgtgaaaac
 
  ccctcattgaagtttgttctctgctctctctttttgggtttagcactgagttttggggtt
 
  tatttcgagacatacccatacaaagtttgatacttttgtgtccccccttatcaagaaaat
 
  tgtggggtttttttttttttttaataagcttcctttaaattttcaatttttattttggag
 
  gaaaagagtgagaatttcagataagaatctatgagccaatgatattctaattcatcttct
 
  tcgtgaagattttgagttgaattccattttcctttttgtcttggtggtttctcattggtt
 
  ttctcgagaatatttgtggttttgggagaagaggcttcactgtagcattgaaaaagtctt
 
  aaacttttctgtgtctttttatgtaagctttgaacagcttcacctttctgggttttctca
 
  gattgtgtctaatcttgaaaaaccttttattcgtagaagcagca
[0149] Promoters associated with any of the potato genes provided below in Example 4 are also provided herewith. In particular, use of the promoter associated with the StCLV1 gene provided below in any of the methods of this invention is provided.

Example 4

Sequences of Various Candidate Potato Nematode CLE Receptor Genes are Provided

[0150] Sequences correspond to potato genes analyzed in FIG. 14 and as described in the claims and Example 2. The ATG start codon and TGA stop codon are underlined.
[0151] 
[00005] [TABLE-US-00005]
  >StCRN cDNA (From clone 4-3:  
  (SEQ ID NO: 6)  
  ATCGCATGGTTTCATGGAGCTCCTTGTTTTTTTGTTGGAATTTGATGATTTTCCAATTTGGTTATTATGTTGTTC  
 
  ATTGTTGTTGTTGAGTCTATTTTGTGGTGGTGCGGAGGTGTGAGCTTTAAATTGGAGTTGGGGTGATTGTTGTTT
 
  TGTTCGCCGGAGAAGCCATCTCCAGTGAGGTTGGTTGGAGAAGGAGAGAGATGAGGAGAGCAATGAGTAATTTCA
 
  ACTATTAAAGATTCGTTTCAGAAAGAGAAAAAAAGAAGAAAATGGTCACATTGTCGTCCTTGTGTAACATTCAGA
 
GGAGTGAACCCTAAACTTGCCGACCCACAGAGAAAAACAACCCTAGTTTCCATGGGGACCTGCTGTAACAGTAGC
 
  ACAGTTCTCAAGCTTTGTTTTTTGTGGCTACAACTAATCTGTGTGCAATGCCATGGAAGGATACTCAAGGATGAT
 
  ACCTCCTCATCTGATCAGTTTAAGAACAGATTTCAAAGGATTTTTCTGAGTATACTTTTTGGTATGTTTACAGGA
 
  TTGATTTGTGCACTTGTTTTTGCTTGGCTTGTTCGGAGTTTTGTTCGTTACATTAACAAAGCCCCAATTCTCAAA
 
  GGCCCTGTTGTATTCTCTCCTAAAATTCCATCCAAAACTCTGCAATCAGCTCTTGCTAATGATACCCAGTTGATA
 
  GGGTCAAGTAGTTCTGGAAAATACTACAGAACTGTTCTTGATAATGGGCTTACTGTTGCAGTTAAGAGAATGGAA
 
  CCTGGTTCTCCACAGTTACATACCAAGTCATTTAAGAGAAGAATACAACACGAACTTGAACTTATTGCTAGTTTG
 
  AGGCATAGGAATTTGATGAGTTTAAGGGCTTATGTTCGTGAATCGAATACGTTCTTTCTGGTTTACGATTATGTA
 
  AACACTGGCAGTCTTGAAGATGTAATGAACAAAGTTAGGGAAAATCAATTGCAACTTACCTGGGAAGTCAGGCTC
 
  CGAATTGCAGTTGGGATTGTTAAGGCTCTTCAGTATCTTCATTTCTCTTGTAACCCCACAGTTTTGCATCGGAAT
 
  TTGAAACCCACAAATGTAATGTTGGATGCTGAGTTTGAGCCTAGGTTGGCTGATTGTGGTTTGGCTAAAATCATT
 
  CCCACTTTAAATCTCCCTGCTGCATCAAACTATGGTCCTCCAGAATCATTCCAGAGTTGCAGCAGGTATACCGAT
 
  AAAAGTGATGTATTTAGCTTTGGGGTTATATTGGGTGTTCTATTAACTGGAAAGTACCCAACAGATCCCTTCTTT
 
  GGGGATACATCTACTGGAGGAAGTCTAGCACGTTGGCTTCAACGCTTGCAGGAAGCAGGCGATGCTCGAGAAGCA
 
  TTGGATAAGAGTATTCTAGGGGAAGAGGTTGAGGAAGATGAGATGTTAATGGCAGTAAAAATAGCAGCGGTATGC
 
TTATCAGACATGCCTGCTGATCGACCTTCCAGTGATGAGCTCGTTTCCATGCTCACCCAATTAAATAGCTTCTGA
 
  TTAATTACTTTGGTCGAGAGGGAAAGCAGTCAAGGATTCAAATAATCACAAGATCTTTAAGGTTGTTCTTTTGGC
 
  TTTCTAAGGTGATAGTTTGCTGTGTGCTTTTGGTAGTTGAGCAATGCCTTTTGGTTATCGCAATGAGCACGAGTG
 
  TAGTTGGC
 
  >StBam1
  (SEQ ID NO: 7)  
TTCTCACTCTCACTGAGTGAATCTGCAAACCAAACAGTTGGTGGGCATTAGATTAAGGAAGGAAAAATGCGTCTT  
 
  CTTTTTCTTCTTCTTCTTGTTATGCATTTTACTGACTTTTCCGCCGGTAAACAACCTCGGTTACCGGAATATCAG
 
  GCTTTGCTTGCCCTGAAAACTGCCATTACCGATGACCCACAGTTAACACTTGCCTCATGGAACATCTCCACCAGT
 
  CACTGTACGTGGAATGGTGTCACGTGCGACACGCATCGTCACGTGACCTCTCTTGATATTTCTGGGTTTAATCTT
 
  ACCGGTACTCTTCCGCCGGAAGTTGGGAATCTTCGTTTCTTACAAAATCTGTCTGTTGCTGTTAACCAGTTTACT
 
  GGACCCATTCCTGTTGAAATCTCCTTTATTCCAAATCTCGGTTACCTTAATCTTTCTAATAACATATTCGGGATG
 
  GAATTCCCTCCGCAGTTAACCCGTCTGCGTAACCTCCAAGTCCTTGACCTTTACAACAACAATATGACCGGTGAA
 
  CTTCCCCTTGAGGTGTATCAGATGACTAACCTTCGACATCTACACCTCGGCGGGAACTTTTTCGGTGGCCGCATT
 
  CCTCCGGAGTATGGAAGGTTCCCGTCTCTAGAGTACCTCGCAGTTTCAGGCAATGCACTGGTAGGAGAGATACCA
 
  CCGGAGATTGGAAACATCACTACACTTCAGCAGTTGTATGTAGGATACTACAATACCTTCACCGGTGGGATTCCC
 
  CCGGCAATAGGGAACTTATCGCAGCTCCTCCGGTTTGATGCTGCTAACTGTGGACTTTCGGGGGAGATTCCACCG
 
  GAGATTGGGAAGCTTCAGAACCTTGACACTCTCTTCCTGCAAGTGAATTCTCTGTCTGGGTCATTAACTCCGGAG
 
  ATAGGTTATCTGAAGAGCTTGAAATCTTTGGATCTGTCGAATAACATGTTCTCTGGCGAGATACCGCCAACATTT
 
  GCGGAGCTTAAGAATATCACTCTTGTTAATCTTTTTCGGAATAAGCTTTATGGGTCAATACCAGAGTTCATAGAG
 
  GACTTGCCGGAGCTAGAGGTGTTGCAACTTTGGGAAAATAACTTTACGGGAAGCATTCCACAGGGGTTAGGCACA
 
  AAGAGCAAGCTCAAAAATGTTGATCTCAGTTCCAATAAATTGACTGGAAATTTACCCCCAAACATGTGTTCCGGT
 
  AACAATCTGCAGACAATTATCACTCTAGGGAACTTCTTGTTTGGCCCAATTCCTGAATCTTTGGGTAGGTGTGAA
 
  TCACTTAATCGGATTAGGATGGGAGAGAATTATCTGAATGGGTCAATTCCAAAGGGGCTCTTAAGCTTGCCACGT
 
  CTGTCACAAATTGAACTTCAGAATAATATTCTCACTGGTACATTTCCTGATATTTCTTCCAAATCTAATAGTCTT
 
  GGGCAGATTATCCTTTCAAATAATCGCCTAACTGGACCTTTGCCGCCAAGCATTGGAAACTTTGCTGTAGCCCAA
 
  AAATTGCTTCTTGATGGGAACAAATTTTCGGGACGAATTCCAGCAGAAATAGGAAAGCTTCAACAGCTATCCAAA
 
  ATTGATTTCAGTCACAACAACTTTTCTGGACCCATGGCTCCGGAGATTAGCCAGTGCAAGTTGCTGACTTATGTT
 
  GATCTCAGCAGGAACCAACTTTCGGGTGAGATTCCTTCTGAGATCACAGGTATGAGGATACTCAACTACTTGAAC
 
  TTATCGAGAAACCACTTAGTTGGGAGTATTCCTTCCCCTATTTCTAGTATGCAGAGTTTAACTTCTGTTGATTTC
 
  TCATATAACAACTTTTCTGGTTTAGTTCCTGGAACCGGGCAATTTAGTTATTTCAACTACACCTCATTTCTGGGC
 
  AATCCAGATCTTTGCGGACCCTATTTGGGCCCTTGCAAAGAGGGTGTTGTTGATGGGGTTAGTCAACCTCATCAA
 
  CGAGGAGCCTTATCGCCTTCGATGAAGCTTTTACTTGTTATTGGTTTGCTTGTCTGTTCTATTGTGTTTGCTGTT
 
  GCTGCAATTATAAAGGCCCGATCTTTAAAGAAGGCAAGTGAAGCTCGTGCCTGGAAGCTCACTGCTTTTCAGCGC
 
  CTAGATTTTACTTGTGATGATATTTTGGACAGCTTGAAGGAGGATAACATTATTGGAAAAGGAGGTGCTGGTATA
 
  GTCTACAAGGGGGTAATGCCGAGCGGGGAACATGTAGCAGTTAAGAGGTTGCCAGCTATGAGCAGGGGTTCCTCT
 
  CATGATCATGGGTTCAATGCAGAGATACAGACTCTTGGGAGGATCCGACACAGGCACATTGTTAGATTATTAGGA
 
  TTTTGCTCGAATCATGAGACAAATCTTTTGGTTTATGAGTACATGCCTAATGGAAGTCTTGGGGAAATGCTTCAT
 
  GGCAAGAAAGGCGGTCATCTACATTGGGATACCAGGTATAAGATAGCCGTGGAGTCTGCAAAGGGTCTTTGCTAT
 
  CTCCATCACGATTGCTCTCCTTTGATCCTCCATCGTGATGTGAAATCAAACAACATTCTGCTAGACTCCAGCTTT
 
  GAAGCTCATGTTGCTGATTTTGGACTTGCTAAATTCTTGCAAGATTCAGGGACATCAGAATGCATGTCTGCTATT
 
  GCTGGTTCTTATGGGTACATTGCTCCAGAATATGCTTACACGCTTAAGGTTGATGAGAAAAGTGATGTATATAGC
 
  TTCGGTGTGGTGCTATTAGAACTGGTAAGTGGCAAAAAGCCAGTTGGAGAATTTGGTGATGGTGTTGACATAGTC
 
  CAATGGGTTAGGAAAATGACTGATGGGAAAAAGGATGGAGTTCTCAAGATCCTTGACCCAAGACTCTCAACGGTT
 
  CCCCTTAATGAGGTGATGCATGTCTTCTATGTCGCATTGTTGTGTGTTGAAGAGCAGGCTGTGGAACGCCCCACC
 
  ATGCGA
 
  > StBam2 (from clone 6-4;)
  (SEQ ID NO: 8)  
CCACCATTGAAGAAACATGCGTTTTCTTCTCCTCTTCTTCCTTTCCCTTATTCTCCATTTCCATCTCCTCCACTT  
 
  CACCACCGCAAAACCACCTTACGTGCCAGAATACCGGGCATTACTCTCCCTGAAAACTGCCATTACCGATGACCC
 
  ACAATCTGCTCTTCTTTCATGGAATATCTCAACAAGTCATTGTACATGGAGAGGTGTCACGTGCGACCGGTATCG
 
  TCACGTGACTTCTCTCGACATCTCTGGTTTTAATCTCACCGGTACTCTCACGCCGGAAGTTGGTCATCTCCGTTT
 
  TTTGCTCAATCTTTCTGTAGCTGTTAACCAGTTCTCTGGACCCATTCCTATAGAGCTCTCGTTTATACCAAATCT
 
  GAGTTACCTTAACCTCTCTAACAACATTTTCAATTTGAGTTTCCCTCCCCAGCTTACCCATCTCCGGTACTTGAA
 
  AGTTCTCGATATTTATAATAACAATATGACCGGTGACCTTCCGGTTGGGGTTTACAATTTGACTAATCTTCGACA
 
  TCTTCATTTGGGTGGCAATTTTTTTAGTGGCAGTATTCCACCGGAGTATGGTAGATTCCCATTCCTAGAATACCT
 
  TGCAGTTTCTGGAAATGCGCTCGTCGGTATGATACCACCGGAGATCGGAAATATTACCACACTTCGTGAGCTTTA
 
  CATTGGATACTACAACACGTTTTCCGGTGGGTTACCGGCGGAAATAGGGAACTTGTCGGAGCTCATTCGGTTAGA
 
  TGCTGCAAACTGTGGACTTTCCGGTGGGATTCCGCCGGAGATAGGGAAGCTTCAGAAATTAGATACACTGTTCTT
 
  GCAAGTGAATGGTCTTTCTGGGTCTGTTACACCGGAATTGGGGAATTTAAAAAGCTTGAAATCTTTAGATCTATC
 
  AAACAATATGCTCTCCGGTGAAATACCGTTCACATTCACAGAGCTGAAGAATCTAACTCTGCTAAATCTTTTCCG
 
  TAACAAGCTTTACGGGTCGATACCGGAGTTCATAGAAAATTTGCCGAAACTGGAAGTATTGCAGCTTTGGGAAAA
 
  CAACTTTACCGGAAGTATTCCACAAGGTTTAGGCAAAAACAGTAAGTTAACAAACGTTGACATCAGTACCGACAA
 
  ATTAACCGGAAATTTGCCCCCAAACATGTGTTCCGGCAACAAGTTACAGACGTTGATCACTCTTGGAAACTTCTT
 
  GTTTGGCCCAATTCCAGAATCTTTAGGTGAGTGTCAATCACTTAATAGGATTAGAATGGGAGAAAATTTTCTAAA
 
  TGGGTCTATTCCAAAAGGGCTATTCAGTTTGCCCAAGCTTTCACAAGTAGAACTTCAAGATAATCTTCTCACTGG
 
  TACATTTCCAGTGACTGGTTCTGTTTCATCAAGTCTTGGACAGATTTGTCTGTCGAATAATCGTTTCACGGGGCC
 
  TTTGCCATCGAGCATTGGAAATTTGACTGGTGTTCAAAAGTTGCTTCTTGATGGGAACAAGTTTTCTGGTCAAAT
 
  TCCAGCTGAATTAGGGAAATTGCAGCAGCTGTCGAAAATGGATTTTAGTGGTAACAGTTTTTCAGGCCTGATTCC
 
  ACCGGAGATAAGCCAGTGCAAGGCTTTAACTTATGTTGATCTTAGTAGGAATAAGCTATCTGGTGAAGTTCCTAC
 
  TGAGATCACTGGTATGAGGATACTGAATTACTTGAATGTATCGCGGAATCAGTTAGTTGGGAGTATTCCTGCACC
 
  TATTGCAGCAATGCAGAGTTTAACCTCGGTTGATTTTTCGTATAACAACTTATCTGGATTGGTTCCGGGTACTGG
 
  TCAGTTCAGTTACTTCAATTACACATCATTTATTGGTAATCCAGATCTTTGCGGACCCTATTTGGGTCCTTGCAA
 
  AGAAGGTATTGTTGATGGGGTTAGTCGACCTCATGAGAGAGGTGCATTTTCGCCTTCTATGAAGCTTTTACTTGT
 
  TATCGGGTTGCTTGTTTGCTCGATTGTGTTTGCTATCGCTGCAATTATTAAGGCTAGATCGTTAAAGAAGGCGAG
 
  TCAGGCTCGTGCCTGGAAGCTTACTGCTTTCCAACGCCTGGATTTCACTTGTGATGATGTATTGGAATGTTTGAA
 
  AGAGGATAACATTATTGGTAAAGGAGGTGCTGGAATAGTATACAAGGGGGTAATGCCAAATGGTGAACTTGTTGC
 
  TGTTAAAAGGTTGCCGGTTATGAGCCGTGGTTCTTCCCATGATCACGGGTTTAATGCCGAGATACAGACACTTGG
 
  GAGTATTCGACATAGACATATTGTTAGATTATTAGGATTTTGCTCAAATCATGAAACAAATCTTTTGGTTTATGA
 
  GTACATGCCTAATGGGAGCCTTGGTGAAATGCTTCATGGAAAGAAAGGAGGTCACTTGCATTGGGATACCAGGCA
 
  TAAGATAGCATTGGAGGCTGCAAAGGGACTTTGTTATCTTCATCACGATTGCTCGCCTTTGATCCTCCATCGTGA
 
  TGTAAAATCAAACAACATTCTTCTGGATTCCAGCTTCGAAGCTCACGTTGCTGATTTTGGGCTTGCCAAGTTTTT
 
  GCAAGACTCGGGAACATCAGAATGCATGTCTGCAATTGCTGGTTCTTATGGCTACATTGCACCAGAATATGCATA
 
  CACACTCAAGGTAGATGAGAAGAGTGATGTATACAGCTTTGGTGTGGTTCTGTTAGAATTGGTGAGCGGGAAAAA
 
  GCCAGTTGGGGAATTTGGTGATGGCGTTGACATAGTCCAATGGGTAAGGAGGATGACCGATGGGAAAAAAGAAGG
 
  AGTTCTAAAGATCCTTGATCCAAGACTCTCAACAGTTCCCCTTCATGAGGTGATGCATGTGTTCTATGTTGCAAT
 
  GCTGTGTGTCGAAGAGCAAGCTGTTGAACGCCCCAAAATGCGTGAGGTTGTGCAAATGCTAACTGAGCTTCCCAA
 
  GCCATCTGGTCCAAAAACAGAAGATTCAACAATCACCGAGTCGCCCCCATCATCAGGTCCTGCATTAGAGTCTCC
 
  CACTTCGACTCCCGGAGACACGAAAGACCAGTACCACCATCAGCCATCACCTCAATCTCCTCCACCTGACCTACT
 
CAGCATATGACCTACAATGTTCCCTTCTAATAGAGGATG
 
  >StER (From 8-16;
  (SEQ ID NO: 9)  
  GTCGGTAAGTCCAAGAACTGGTTTTTCAATTCAAAGGAGCTGAGTTAGTGTAAACACTTTTGGTTTTGAGTTTTG  
 
ACAGAGACTTGAGTCTCAGAGAAACTACCATGGCATCATTTTTACTTCAAAGATGTAATCTTTTCTTTGAGGTTC
 
  TTCTTCTTTTGGGGTTCTTGATTTTCTTCAGCTTTGGTTCTGTGGTGTCTGATGATGGTTCTGCATTGTTGGAGA
 
  TTAAGAAGTCAATTAGGGACATGGAGAATGTGTTGTATGACTGGACTGATTCTCCTTCATCTGATTACTGTGCCT
 
  GGAGAGGTGTTACCTGTGATAATGTCACCTTCAATGTTGTTCAACTTAATCTTTCGAGTTTAAATCTTGATGGGG
 
  AGTTGTCTCCTGCAATTGGACAGCTCAAAGGCCTTATATCTATTGATGTTAGGGGAAATCGCCTTTCTGGCCAGA
 
  TACCAGATGAGATTGGTGACTGTTCAGCACTGAAAAACTTGGACCTATCCTTCAATGAGCTTTATGGTGATATTC
 
  CGTTTTCCATATCAAAACTTAAGCAACTGGAATATCTGATTATAAAGAACAATCAGTTGATTGGACCAATTCCAT
 
  CGACATTGTCACAGATCCCCAACTTGAAGGTCTTGGACCTGGCTCAAAATAGGTTAAGTGGAGAAATTCCTAGGC
 
  TGATATACTGGAATGGAGTCCTGCAGTATTTGGGACTGCGTGGCAACAACTTGGGTGGATCACTTTCTCCTGATA
 
  TGTGTCAGCTCACCGGCCTGTGGTACTTTGATGTTCGGAACAATAGTTTGACTGGTTCCATTCCTCAAAATATTG
 
  GCAACTGTACTGCTTTCCAGGTTCTAGATTTGTCTTATAATGACTTGACTGGAGAGATTCCTTTCAACATTGGTT
 
  TCCTGCAAGTAGCGACCTTGTCTTTGCAAGGTAATCGCCTTTCAGGGCAGATCCCTTCTGTCATTGGATTGATGC
 
  AAGCTCTTGCAGTTTTGGACTTGAGCTGCAATATGTTGAGTGGAACAATTCCTTCAATTCTTGGGAATTTGACTT
 
  ACACAGAAAAATTGTATCTACATGGGAACAAGCTATCTGGTTCCATTCCTCCAGAGCTGGGAAATATGACAAAGC
 
  TTCACTACTTAGAATTGAATGATAACCAACTTACTGGACGCATACCACCAGAACTTGGAAAGCTGACGGAGTTGT
 
  TTGACTTAAATGCTGCAAACAACCACCTTGATGGGCCCATTCCTTCCAATCTTAGCTCATGTACCAATTTGAATA
 
  GTCTCAACGTTCATGGAAACAAATTGAATGGTACGATTCCACCTGCTTTTCAAAAGCTGGAAAGTATGACCTATC
 
  TTAATCTCTCCTCCAACAACCTCAAAGGCCCAATTCCAATTGAGCTTTCTCGTATTGGGAATGTAGATACACTGG
 
  ACTTGTCAAACAACAGGATCAGTGGTCCTATACCTTTGTCCCTCGGTGATTTGGAACATCTTCTTAAACTGAACT
 
  TGAGCAAGAACGAAATAAATGGAAACTTGCCAGCTAAATTTGGCAATTTAAGGAGCATCATGGAGATTGATCTGT
 
  CAAGCAATCACCTCTCTGGTCCCTTGCCTCAGGAACTTGGTCAGCTTCCAAATCTGTACTTGCTGAAACTGGAAA
 
  ACAACAATTTATCAGGCGATGTGATGTCCTTAGCCAGTTGTCTCAGTCTAAATGTCCTAAATGTCTCGTACAATA
 
  ATCTGGGAGGGAATATTCCAACAGGCAATAATTTCTCTAGATTTTCACCAGACAGCTTCATAGGAAATCCAGATC
 
  TGTGTGGGTATTGGCTCACTTCTCCTTGTCATGCATCTCATCCAGCAGAGCGAGTTTCAATTTCTAAAGCTGCTA
 
  TACTTGGTATTGCTCTGGGTGGCTCGGTGATTCTTCTGATGATACTAGTAGCAGCATGCCGGCCACAGAATCCTG
 
  CACCTTTCATGGAAGGATCTATTGATAAACCAGTTTATTACTCATCTCCAAAGCTTGTGATCCTTCATATGAACA
 
  TGGCACTTCATGTTTACGAGGACATTATGAGGATGACTGAGAACTTGAGTGAGAAGTATATAATTGGTTGTGGAG
 
  CATCAAGTACGGTATATAAATGTGTTTTGAAAAATTGCAAGCCTGTAGCTATCAAGAAATTGTACTCTCACAACC
 
  CGCAATACTTGAAGGAATTTGAGACTGAACTTGAGACAGTTGGGAGTATTAAGCATCGTAATCTTGTCTGCCTCC
 
  AAGGATATTCTCTTTCTCCATCTGGCCATCTTCTTTTCTATGACTACATGGAAAATGGTAGCCTTTGGGATTTGC
 
  TTCATGGTCCTACAACAAAGAAGAAAAAGCTTGATTGGGTTACTCGCCTTCGAATTGCATTGGGATCAGCTCAAG
 
  GGCTTGCATATCTTCACCATGATTGTAGCCCTCGAATTATCCACCGTGATGTTAAATCATCAAATATCTTGTTGG
 
  ACAAAGACTTTGAGGCTCATCTGACTGATTTTGGCATTGCCAAAAGCTTATGCATATCAAAGACCTATACGTCCA
 
  CATACATTATGGGAACCATTGGTTACATTGATCCAGAGTATGCTCGCACTTCTCGCTTGACAGAGAAGTCTGATG
 
  TTTACAGCTATGGAATTGTTCTATTGGAATTGCTCACTGGAAGGAAAGCTGTAGATAATGAGTCTAATCTACACC
 
  ATATGATTCTAACTAAGGTAGCAAACAATGCTGTAATGGAAACAGTGGATCCTGAGATCACAGGCACATGCAAAG
 
  ATCTTGCAGATGTGAAGAAGGTTTTTCAGCTTGCCCTTCTATGTTCCAAAAGACAGCCTGCTGAGAGACCAACAA
 
  TGCATGAAGTGGCAAGAGTACTTGAAAGCCTAATACCCGTCACTGAAATGAAACAGCCAAATCCAACGCTCTCAC
 
  TTGCATTACTTCCATCTGCTAAGGTACCTTGTTACATGGATGAATATGTCAACCTCAAGACACCCCATCTAGTGA
 
ATTGTTCATCCATGAGCATTTCAGATGCTCAACTTTTCCTGAAGTTTGGAGAGGTCATATCCCAGAATAGTGGCT
 
GAAAATAACATGAGTAGATTTCTTGGGATTGTGTAAAAAAATGTAGTGCCATTATAATATTATTATTGTAGGTAG
 
  TTGTTGTAAGATGATGCATGCAATAGTGGTCCAGTCTACTTTTTCCACTACATAGGTCTAGTGTGTGTAAAAATA
 
  TTTCACTTTTTACCATGATGAAATTGGAAGAGGTAGCACTTGGTAGAGTATTGTAATATTGGTTTTTGGGACTGA
 
  TGCTGAGTATGGACTATACTGTCTGTAGGATTTTTGGCACACACTTTGAGGTGGCCTTAGCA
 
  >StCLV1 (From clv1 clone 11-1 041710;
  (SEQ ID NO: 10)  
AGACTAAACTAACAGTGTAATAATGTCACTCCCCAAAAAAATATCCCTTTTCCTCCAAATTTTCATTTTTTTTGT  
 
  TTTCTCCATTAATGCAAACTCTGATCTTGAAACCCTTTTGAAGCTCAAAGAATCCATGGTTGCTCCTGGAACTTC
 
  TGCACTTCTTGATTGGAACAACAACACAAATTACCCTTTTTCCCATTGTTCTTTTTCTGGTGTTACATGTAACAA
 
  TAACCCTCATGTTATATCTATAAACATCACTAATGTTCCTCTATTTGGTACTATTCCACCTGAAATTGGTCTTTT
 
  ACAAAATCTTGAAAATCTTATTATTTTTGGTGATAATATTACTGGTACACTCCCTTTAGAAATGTCACAACTTTC
 
  TTCTATTAAACATGTTAATCTTTCTTACAACAACTTTTCTGGTCCTTTTCCTAGAGAAATCTTGTTGGGGTTAAT
 
  AAAGCTTGAATCTTTTGACATTTATAACAACAATTTCACTGGTGAACTTCCTACTGAGTTTGTAAAGTTGAAAAA
 
  GTTGGAAACTTTACATCTTGGTGGAAACTATTTTCATGGTGAAATACCAGAAGTTTATTCTCATATTGTAAGTTT
 
  AAAGTGGTTGGGTTTAGAGGGAAATTCACTAACTGGGAAAATACCAAAGAGTTTGGTTTTGTTACCAAATCTTGA
 
  AGAACTTAGATTGGGCTATTATAATAGTTATGAAGGGGGTATTCCATCTGAGTTTGGTAATATTAGTACACTTAA
 
  ACTTCTTGATCTTGGAAATTGTAATCTTGATGGTGAAGTTCCTCCAAGTCTTGGAAATTTGAAGAAGTTGCATAC
 
  TTTGTTTCTACAAGTGAACAGACTTACAGGTCGCATACCTTCTGAACTATCTGGTTTAGAGAGTTTGATGTCGTT
 
  TGATTTGTCTTTTAATCAACTGACCGGAGAAATACCAGAGAGTTTTGTGAAGTTGCAGAATTTGACATTGATTAA
 
  CTTGTTTAGAAACAACTTGCATGGTCCAATTCCCCCTTTTATTGGTGACCTTCCAAATCTTGAAGTGTTGCAGAT
 
  TTGGGGAAACAATTTTACTCTTGAATTGCCCGAAAATCTTGGGCGTAACGGGAGGTTTTTGTTTCTTGATATTTC
 
  TATTAATCATTTTACTGGAAGGATACCACCTGATTTGTGTAGAGGAGGGAAGTTAAAGACACTGATTCTAATGGA
 
  AAATTACTTCTTTGGTCCAATTCCTGAACAACTTGGTGAGTGCAAATCGCTTGCTCGAATTCGCGTTAGGAAGAA
 
  TTACTTAAATGGTACTATTCCAGCTGGTTTTTTCAAGTTACCTGCATTGGATATGCTTGAACTTGACAACAACTA
 
  TTTCACTGGTGAGCTGCCAACGGAGATAAACGCGAATAATCTCACTAAACTTGTACTTTCCAACAACTGGATCAC
 
  GGGGAACATTCCTCCATCATTAGGGAACTTGAAGAATCTAGTCACTCTATCACTTGATATGAACAGGTTATCTGG
 
  TGAAATTCCTCAAGAAATTGCGAGTTTGAATAAACTCGTGACCATCAACTTGAGTGGCAACAATTTAACAGGTGA
 
  AATCCCAAGTTCAATTGCGCTTTGTTCAGAGCTAACATTGGTTGACTTGAGCAGAAACCAACTGGTTGGTGAAGT
 
  GCCAAAAGAAATCACCAAGTTAAATAGCTTGAACGCTCTGAACTTGTCAAGAAACCAACTGAGTGGCGCCATTCC
 
  TGGAGAAGTCGGAGTGATGAATGGCTTGACAGTTTTAGATCTTTCTTACAATGATCTTTCTGGAAGGAGACCGAC
 
  CAACGGACAACTAAAGTTCTTCAATGACACTTATTTTGTAGGAAATCCAAAACTCTGTTCACCTCATGCTACTTT
 
  TTGCCCGTCAGCCTCCAATTCACCACAAAACGCGCTCAAAATCCATGCTGGGAAGTTCACAACTATCCAATTGGT
 
  GATTACAATAATCATCTTAGTCACTGTTGCATTGCTGTTGGCAGTTACCGTGTTGTTCATCAAGAAGGAAAAGTT
 
  CAAGAATTCGAAACTTTGGAAGTTAACAGCATTCCAGAAACTTGATTTCAGAGCTGAGGATGTTTTGGAGTGTTT
 
  AAAAGAGGAGAACATAATTGGGAAAGGTGGAGCTGGCGTTGTGTACCGAGGGTCTATGTCAAATGGCATCGACGT
 
  TGCAATTAAGAAACTTGTAGGCCGAGGAACTGGACACCATGATCATGGATTCTCAGCTGAAATCCAAACACTAGG
 
  AAGGATCAGGCACAGAAACATCGTACGATTACTAGGATATGTCTCAAACAAAGACACAAACTTGTTGTTGTACGA
 
  ATACGTGTCGAATGGGAGCTTAGGTGAAATGTTACATGGTGCCAAAGGAGCACATTTGAAATGGGAGACGAGGTA
 
  CCGTATTGCTGTGGAAGCTGCAAAGGGATTGTGTTATTTGCACCATGATTGTTCGCCTTCGATTATTCATAGAGA
 
  TGTCAAGTCCAATAATATTCCGCTGGATTCCGATTACGAGGCTCATGTTGCTGATTTTGGCCTAGCCAAATTCTT
 
  GCAGGATGCTGGTGCATCAGAGTGCATGTCCTCTATTGCTGGCTCATATGGTTACATTGCTCCAGAGTATGCATA
 
  CACATTGAAAGTTGACCAAAAGAGTGATGTATACAGTTTTGGAGTTGTACTGTTGGAACTTATCACAGGTCACAA
 
  GCCAGTTGGTGAATTCGGGGACGGTGTAGATATAGTCAGATGGGTAAATAAAACAATGTCCGAATTATCTCAGCC
 
  GTCTGATGCAGCCTCAGTTTTAGCAGTCGTTGACTCGAGGCTACATAGTTACCCTCTTGCAAGTGTTGTAAATTT
 
  GTTCAAGATTGCTATAATGTGTGTTGAAGAAGAGAGTTGTGCTAGGCCTACTATGAGGGAAGTTGTTCACATGCT
 
TACAAATCTTCCTCAGTCTACTACTACTACTACTACTACTCTCCTTGCCCTTTGAAATTGCACCGATATCAAGTG
 
  TCTGGTTGAAAACTCGTGGAGTTTGAGGCCGGGAACACGAGTCTCATGAGTCTATTTGGGTACGGGGAACAA
 
  >StCLV2 (From clv2-7;
  (SEQ ID NO: 11)  
ATGGCAGAATCAGTTCTTGAACCTTGTACAACCTCTTATTCCTTCAAAGTTTCAATCTTTATCCTATTCTTCTTG  
 
  ATTTTCCCTTTCTTGAACCCATTTTCATCTGCATTTCCTCTTTCTTTTGATACTAATGCAACTGAGGCTGTCAAT
 
  CTTGAAACAGAAGAGGACATGGGTTTGCTTTTGTTCTTCAAGTTACAGTTTCGAGAAACCCCTTTACCAAGCTGG
 
  GATGTCAATGTTCCTCTATCAAACTGGACTGGTGTTACCCGGTCTAACCAGACCGGACGGGTCACTGGACTTAAC
 
  CTCACAAGGTTTAACTTGTCAGGACAGGTTCATCCTTGTTTGTGTAATCTTACTTTTCTTGAAACCCTTGTGTTG
 
  TCTCATAATAGCTTTAACAATTCAATACCTTCTTGTTTATGGAAGTTGTGGAGCCTTAAGACCTTAGATCTTAGC
 
  TATAATATGCTTACTCTTCTTATTCCTAGTACATTTGCAACAACTATGAGTAAGTTAATTGAGCTTGACCTTAGT
 
  CATAACATGTTGAGTGATGAAATCCCAATGTGGATAGGGAATGTCTCAATGTCACTTGAAAAACTTAACTTAGGG
 
  TTTAATAGTTTTCATGGGGATATACCTAAGAGCTTGTTGAATTTGATGTCTTTGAAGTATCTTGACTTGTCTCAC
 
  AATAGTTTGATGGGAAATGTGGGTGATTTTAACCAAGAATTGGTCTCACTTAATCTTGAGTCTAATTTATTATCG
 
  GGTACTTTGCCTTGTTTATATTCGTCAAGGGAATCACTTACACTTCTTAATTTAGCAAACAATTCGATTCTTGGA
 
  GGTATACCAACGTGTATCTCGAGTCTTGGGGGTTTGACACAGCTCAACTTGTCACGTAATGAATTACGATATGGT
 
  ATCTCGCCTAGACTGGTTTTTTCAGAGAGGTTATGTTTGTTGGACTTGAGTTATAATGAGCTATCAGGGAAGATT
 
  CCAAGTAGGATTGTTGAGGCATCGGACAAGTCTGGACTTCTACTTCTTGACCTGTCTCACAATCAGTTCTCTGGT
 
  AATATTCCTGTAACGATAACAGAATTGAAGAGCTTGCAAGCATTGTTTCTGTCTTATAATCTTCTTGTGGGCGAA
 
  ATACCAGAAAGGATTGGTAATTTGACCTATCTACAGGTGATTGATCTCTCACATAACTTCCTCACCGGCTCGATT
 
  CCTTTGAACATCGTAGGATGTTTCCAACTACTGGTGCTGATACTAAACAGTAATAATCTTTCTGGGGAAATTCAG
 
  CCAGTGCTTGATGCGTTGGATAGTCTTAAGATATTTGATATAGGAAACAACAAGATTTCTGGTGAGATCCCACTG
 
  ACATTGGCAGGCTGCAAGTCGTTGGAAGTTGTTGACTTGAGCTCTAACAATCTCTCAGGGTCTCTAAATGGTGCA
 
  ATAACCAAATGGTCGAACCTCAAATTCCTCTCCCTTGCTCGGAACAAGTTCAGTGGATCTCTGCCAAGTTGGTTG
 
  TTTACATTTCAGGCTATTCATACTCTGGATTTTTCTGGAAACAAGTTCTCGGGATATATACCAGATGGTAACTTT
 
  AACACTAGTCCAAATTTCTACAACGGCGACATTAGGAAAACCATTCCTGCAGTACCATCAATTTCAGCTCGAAGC
 
  CTGGATATCAAACTTTCACTCATTGCTGATGAAACTAGTTTGAGCTTCAACTATAACCTGACAACCACAATTGGA
 
  ATTGATCTGTCTGACAATTTGCTTCATGGTGAAATTCCAGAGGGTCTGTTCGGATTACATGGTTTGGAGTACCTT
 
  AATTTGTCATACAATTTTCTTAATGGTCCAGTTCCAGGGAGTTTAGGGAAGTTGCAGAAGCTAAAAGCACTTGAT
 
  TTATCACATAATTCTTTATCTGGCCACATCCCTGAAAACATTACTGTCCTCAGAAATTTGACAGTTTTAAATCTG
 
  TCTTATAATTGTTTCTCTGGTGTTATTCCGACAAAGCGAGGTTATTGGAAATTTCCTGGAGCATTTGCTGGGAAT
 
  CCAGACTTATGTATGGAATCATCTGGTAATGTCTGTCAAAGAACTTTGCCTGTAGAGCCAGGGAAGAAATTTGAA
 
  GAGGAAATGGAAGAGGGACCATTATCAGTTTGGATTTTCTGTATAAGTGCTTTAGTTAGCTTCTATGTTGGCATT
 
GTTGTTTTATTTTGTTCATCTCGAACAAGAAGCTGTATTCTGCAAACAAAAAGTTTAGCAGGTTGA
 
  >StACR4 (From ACR4-3;)
  SEQ ID NO: 12  
ATGTCTTCAATTGCTATTTCATATGGTGAATATGGTTCTGTTTTTTGTGGGTTGAAGTCAGATGGATCTCATTTG  
 
  GTCAGCTGCTATGGCTCTACTTCTTCTATAATATATTCAACTCCAGCTCATTTCCCTTTTATTGGTCTTACTGCT
 
  GGAAATGGCTTTGTATGTGGACTTTTGATGGATTCTTACCAGCCTTATTGTTGGGGGAAAAGTAATTTTGTACAA
 
  ATGGGAGTGCCTCAGCCTATGATCAAAGGGTCTCAATACTTGGAAATATCTGCAGGTGAAAATCATTTGTGTGGA
 
  CTAAGGCAACCTTTAATGGGGAAGCATAGGAACACTTCACTTGTTGATTGCTGGGGTTATAACATGACCACAAAT
 
  AATGAGTTTGAAGGTCAGATCCACTCTATTTCAGCTGGTTCTGAGTTTAATTGTGCTTTGTTTTCTGTCAATAAA
 
  AGTGTTTTATGTTGGGGGGATGAAACTAGTAGCCAGGTTATTACCCTAGCACCAAAAGATTTGAGATTTATTAAG
 
  ATTGCAGCTGGGGGATATCATGTTTGTGGGATCCTAGAAGGGGTGAATTCTCAAGTGTATTGCTGGGGAAGGAGC
 
  ATGAACCTTGAAGAAGAATTCTCTGTTGCTCAACTCAATGTTGAATTGGCAGCCCCTAGTGATCCAATTATATCT
 
  GTTGTTGGTGGTAAGTTTCATGCTTGTGGGATTAGGAGCTATGACCGTCATGTCGTTTGCTGGGGTTACAGAGTT
 
  GAGAAAAGCACACCACCTCCTAGTGGAGTTAGGCTTTATGAGATAGCAGCTGGTGACTACTTCACTTGTGGTATC
 
  CTTGCGGAAATTTCACTTTTGCCTGTTTGTTGGGGGTTTGGTTTTCCCTCATCGCTACCACTCGCTGTTTCTCCT
 
  GGAGTCTGCAAGCCTAGACCCTGTGCATCTGGCTTCTATGAGTTTAACAACGGAAGTGCAACTTGCAAGTCTCCT
 
  GATTCTCGCATTTGCCTTCCCTGCACCAATGGCTGCCCTGCTGAAATGTATCAACAGGTTCAATGCACTTCATCT
 
  ACGGACAGTCAGTGCACGTATAATTGTTCAAGTTGTACCTCTGTTGACTGCCTAAACAGCTGTTCTACTGCTATT
 
  TCTGGGAAGAAGAACGCTAAATTTTGGTCACTCCAGTTACCAGTAATTGTTGCTGAGGTTGCATTTGCAGTATTC
 
  TTGGTGAGTGTTGTATCTCTAACTTCGATCGTATATGTTCGCTACAAATTAAGGAACTGTAGATGTTCAGGGAAA
 
  GGTCCTAGTCCTAGGAAGAATGGTACTTTCCCAAAGGAAATTGCTAAAGATAGGGCTGATTTGGATGATCTTAAA
 
  ATAAGGAGAGCTCAGATGTTTACTTATGAAGATCTTGAGAGAGCAACTGAGGGATTCAAAGAAGAATCACAAGTT
 
  GGAAAGGGTAGCTTTTCGTGTGTTTTCAAGGGCGTTTTGAAGGACGGTACTGTGGTTGCTGTCAAGAGGGCTATA
 
  ATGTCATCTGACATGAAGAAGAATTCAAAGGAGTTCCACAATGAGCTAGACTTGCTGTCCAGGTTGAATCATGCT
 
  CATTTGCTCAATTTGCTAGGTTATTGTGAAGAAGGTGGAGAGAGACTTCTAGTTTATGAGTACATGGCTAATGAC
 
  TCGTTGCATGAACATCTACATGGGAAAAAGAAGGAGCAATTGGATTGGATAAGAAGGGTAACCATTGCAGTCCAA
 
  GCTGCTCGGGGAATCGAATATTTGCATGGTTATGCATGTCCACCTGTGATTCACAGAGACATCAAGTCCTCAAAC
 
  ATCCTTATAGATGAAGAACACAATGCTCGAGTAGCTGATTTTGGGCTTTCCTTGCTTGGACCTGCTAATAGCAGT
 
  TCCCCATTAGCTGAGTTACCAGCAGGGACACTTGGGTACCTTGATCCCGAGTACTACAGACTACATTATCTTACA
 
  ACCAAATCTGATGTCTATAGCTTTGGTGTTTTGCTTTTGGAAATTCTCAGTGGTCGGAAAGCTATTGACATGCAA
 
  TACGATGAAGGGAACATAGTGGAATGGGCAGTCCCATTAATCAAAGCTGGTGAAATAGAGGCAATACTGGATCCA
 
  GTTTTGAAATCACCTTCTGATGCTGAAGCTCTTAGAAGAATCGCTAATATAGCCAGCAAATGCGTGAGGATGAGA
 
  GGGAAAGAGAGGCCGTCAATGGATAAAGTAACAACAGCTTTGGAGAGAGCACTTGCTCAATTGATGGGTAGTCCA
 
  AGCAATGACCAGCCTATCTTGCCAACAGAGGTTGTTCTAGGAAGCAGCAGAATGCACAAGAAGTCCTCATCAAAT
 
  CGATCAACATCAGAAACAACAGATGTTGCAGAAACTGAGGATCAGTGGTATGTCGAATTCAGAGCTCCTTCGTGG
 
  ATTACATTCCCAAGTGTAGCATCATCTCAGAGAAGAAAGTCTTCAGTATCGGACGCAGATGTTGAAGCAAAGAAT
 
  TTAGAAAGTAGGAACTGTGGAAATGGAACTGATGGATTGAGAAGTTTGGAAGAAGAAATTGGACCAGCTTCTCCT
 
CATGAACATTTGTTCTTGAAACACAACTTCTAA
 
  StERL2
  (SEQ ID NO: 13)  
ATGGAGGTGAGCGTGAAGATGAAATTCCCCTCACAAGCACTACTGTTGGCTCTATTGCTT  
 
  GTTTTACCGATCGTTTTAGCTCTCACCGAAGAAGGCAAAGCATTAATGTCGATCAAGGCA
 
  TCGTTTAGCAACGTGGCAAACGTGTTGCTAGATTGGGATGATGTCCACGACGAGGATTTT
 
  TGCTCATGGCGAGGCGTGTTGTGTGGAAATTTCTCCATTTCCGTCGTTGCCCTTGATTTG
 
  TCTGATAACTTGCTCTATGGAGATATACCTTTCTCAATTTCTAAGCTCAAGCAGCTAGAG
 
  TTATTGAACCTGAAAAACAACCAGTTGTCTGGCCCAATCCCATCCACATTAACTCAAATC
 
  CCTAATCTAAAGACGCTTGGCTTAAGAGGCAACATGTTGACAGGAACATTGTCCCCTGAT
 
  ATGTGCCAGTTGACTGGTTTGTGTGATGTGCGGGGCAATAACCTCAGTGGAATAGTTCCA
 
  GATAATATTGGGAATTGTACAAGTTTTGAGATACTGGATATCTCATACAATCAGATAACT
 
  GGAGAAATTCCCTACAATATTGGATTTTTACAAGTGGCTACCTTGTCTTTGCAAGGAAAT
 
  AGGCTAACTGGGAAGATCCCAGAAGTGATTGGTCTAATGCAAGCTCTTGCTGTTCTGGAC
 
  TTGAGTGAAAATGAGTTGGTGGGACCAATTCCTCCAATCTTTGGCAATTTATCCTACACT
 
  GGGAAACTGTACCTGCACGGCAACAAACTTACAGGGCCAATACCACCGGAGCTAGGAAAT
 
  ATGTCTAAACTTAGTTACTTGCAATTAAATGACAATCAGCTAATGGGGCGAATTCCCTCC
 
  GAACTTGGCAAACTGGACCAGTTATTTGAATTGAATCTTGCAAATAACAAGTTGGAGGGA
 
  CCAATTCCTGAAAATATCAGCTCCTGCTCGGCATTGAATCAACTTAATGTTCATGGCAAC
 
  AACTTAAACGGGTCCATTCCTTCAGGGTTTAAGAATCTTGAGAGCCTGACATATCTGGAT
 
  CTCTCTGGCAATGAATTTTCTGGGTCTATCCCTGGTTCTATTGGAGATTTGGAGCATCTC
 
  CTCACACTGAATCTGAGCAGCAATCATCTTGATGGACAAATTCCTGTAGAATTTGGCAAT
 
  CTGAAAAGTATACAGACCATTGATATGTCATGCAACAAGATTTCTGGTGCCATCCCAAAA
 
  GAGCTGGGACAGCTGCAGACCATGATAACTCTGAATATATCCTACAACAATTTTAGTGGT
 
  GTTGTTCCTCTTTCACGGAATTTCTCGCGGTTTGCACCTGACAGCTTTTTGGGGAACCCA
 
  TTTCTTTGTGGCAACTGGAAAGGCTCAATATGTGACCCCTATGCACCAAGGTCTAACGCC
 
  TTGTTCTCTAGAACAGCTGTTGTTTGCACAGCATTGGGTTTCATAGCACTCTTATCCATG
 
  GTTATAGTGGCAGTGTACAAGTCCAACCAACCACACCAGTTTCTGAAGGGGCCTAAGACC
 
  AATCAAGGTTCCCCCAAACTTGTGGTTCTTCACATGGATATGGCCATCCATACATATGAT
 
  GACATTATGAGGATTACTGAGAACTTCAATGAGAAATTCATCATAGGATATGGTGCTTCC
 
  AGCACTGTATATAAATGTGTTTTGAAAGATTCCCGACCGATTGCCGTTAAGCGACTTTAC
 
  ACTACACATCCGCACAGCTTGCGAGAGTTTGAGACTGAACTGGAGACCATTGGAAGCATC
 
  AGGCATAGAAACCTTGTTAGCTTGCATGGTTACTCCCTTTCCCCTCATGGGAATCTCCTT
 
  TGTTACGACTACTTGGAGAATGGTTCACTCTGGGATCTACTTCATGGGCCTTCCAAAAAG
 
  GTGAAGCTTGACTGGGAAACACGTCTGAGGATTGCTGTTGGTGCTGCTCAGGGTCTTGCT
 
  TATCTTCACCACGATTGCAACCCAAGAATCATCCACAGAGATGTGAAATCTTCAAACATT
 
  CTTGTTGATGAAAATTTTGAGGCTCATCTTTCTGATTTTGGGGTTGCAAAATGCATCCCT
 
  TCTGCAAAAACTCATGCATCAACTTTGGTGTTGGGCACCATAGGTTACATTGACCCTGAG
 
  TATGCCAGGACTTCCAGGTTAACTGAAAAATCAGACGTCTACAGCTTTGGCATTGTTCTC
 
  CTAGAGCTTTTGACAGGAAAGAAACCGGTTGATAATGACTTGAACCTGCATCAGCTGATA
 
  ATGTCAAAGGCGGATGATAACACCGTGATGGATGCTGTTGATCCTGAGGTATCTGTTACA
 
  TGTATGGACTTAACACATGTGAGGAAAACTTTTCAGCTTGCGTTGCTGTGCACAAAAAGA
 
  TTTCCATGTGAGAGGCCAACGATGCATGAGGTTGCTAGGGTACTTGTTTCCTTGCTTCCT
 
  CCCCCGCCAACCAAACCTTGTTTAGACCCACCTCCCAAATCCATTGATTATACAAAGTTT
 
  GTGATTGGGAAAGGACTACCGCAAGTTCAGCAGGGTGATGATTCCTCCGAAGCACAGTGG
 
  CTTTTTCTTAGATATTTAGCTGCTGCACTGGTTCAATGGAACGAGTTTGAAGATGGTGAA
 
GAATTGCATCTATGTTGA

Example 5

Inhibition of Plant Responses to Nematode CLE Peptides and Inhibition of Nematode Infections by Inhibition of a Plant CLV2, CRN1, and/or BAM1 Gene

[0152] Mutant Arabidopsis plants i) homozygous for the recessive bam1-3 mutation; ii) homozygous for the recessive clv2-6 mutation; iii) homozygous for the recessive crn-1 mutation; iv) homozygous for both the recessive clv2-6 and bam1-3 mutations; and v) homozygous for both the recessive crn-1 and bam1-3 mutations were exposed to the cyst nematode Heterodera schachtii and assayed for a response as described in Example 1. More specifically, sterilized receptor mutants were plated in 12-well Falcon tissue culture plates (BD Biosciences) containing modified Knop's medium with 0.8% Daishin agar in a randomized block design. Plants were grown at 24° C. with a 12 hour photoperiod. Fourteen days after germination, seedlings were inoculated with 200 surface-sterilized BCN (Beet Cyst Nematodes; i.e. Heterodera schachtii) J2. J4 females were counted at 14 days post-inoculation (dpi) and adult females were counted at 30 dpi. The average values were calculated and significant differences were determined by using Student's t test (P<0.05). To measure syncytia size, receptor mutants were germinated on modified Knop's medium in vertical square plates and inoculated at 10 days after germination with 10 surface-sterilized BCN J2. At 14 dpi (days post infection) and 30 dpi, syncytia that were transparent and only fed upon by only one nematode were visualized with a Nikon Eclipse TS 100 inverted microscope. Area of syncytia was measured using Adobe Photoshop CS5 and significant differences were determined by using Student's t test (P<0.05). In these experiments, the bam1-3 mutant exhibited a 25% reduction in nematode infection that was similar to reductions obtained with clv2-6 mutants. The clv2-6, bam1-3 double mutant and the crn-1, bam1-3 double mutant showed a 35% and 50% reduction in nematode infection respectively (FIG. 15).

Example 6

Use of a pCLV1 Promoter to Drive Expression of Heterologous Genes in Nematode Infected Roots

[0153] The promoter for the Arabidopsis CLV1 gene was operably linked to a beta-glucuronidase gene (GUS) and introduced into transgenic Arabidopsis plants. The transgenic plants were then infected with BCN and expression of the GUS observed. It was determined that the pCLV1 promoter can provide for expression in the root vasculature uninfected plants as well as upregulation of expression at sites of BCN infection in plant roots (FIG. 16). The sequence of the pCLV1 promoter is provided in Table 4.
[0154] 
[00006] [TABLE-US-00006]
  TABLE 4
 
Arabidopsis thaliana Clv1   gaagacccaaggcccaacgacctactggtcca
  promoter and 5′UTR   ggttgactatgaacaaaagaactagatttttttttcc
  (SEQ ID NO: 14)   cctacattttaaagaaaatacttgatgaagatgtg
    gtgccttttcataagatctaaaaagtttcaaatcttta
    cgatggaacaaaaagtgaaaggtgaagtaagg
    gtcatttgggattgagaagtttcttcgtccaaaatca
    ttgcatgagttgaatagatttgggattaaagctgcc
    aatacaagaggattcggtaatgactgaagcaaa
    agcccagcagggccattaggcaaacaccagttt
    ccaagacggatttgtgtaagaccacttatgacac
    aagtttgtcttcactatcatcatcttcttcttctacttcta
    ctactacttttgtagccttgtcgtttttatcattaacatg
    attgacaagactatgacctatatatcttattattatca
    ttgctctctctatttgtttatattgattattactttttgagat
    ttttcaatggttttatctctaactaaacattataattagt
    gaaacaagcttagtagaagtaaaagtattattcta
    tgctaaagtacattgattagtagagtgtgtaattgtg
    tatacagataatctataaacaattggtgcatctgtat
    ataaaactttatgatttatttattgtatttaataagtata
    tgaattgggtacctaactttctaaacagttccaaatt
    tattcttaaatcaaattgcatatgatttttaaatattttg
    agacgattttattataacgcaaacaacagagtaa
    aagaagcatatgttgcaaattgtactatggcaagt
    tcaaatcgaaacatttttgtgaaaatcaaacatgtg
    aaccaagcttctacagtttaattccctttcgtataatt
    taatttcaacaaatttattgatatccatctagaaattg
    gtccaaagttctttcacccttgagtcatttagtgata
    aagatgacatgatttttggtgataaattttccatcgtt
    gctatatgtcgttatattattctcctatatgtatattata
    ctatttacatcagaaaataatccaaagtttagagat
    tcttttttacaataataaaatttcccacttactaaaaa
    gagctccttttctgctgaagagaacctaaaccttta
    ttcccaaagttcattgagttagagcattttcagcga
    atcacataagagatgctctcttcttcatcactaattg
    acatctcattgttttaaaggttgcacttgtacctgttg
    atctgattctcaatccacttaagttaaaccaaatag
    acacgagaaaaaagcacatttatttgttgctaagt
    atgcatatttttcagcgtttacttcttaatctaatgtata
    tcataagataatatctaaaagagaatgcacaaa
    agattattaatatgagaaattcgctgccatttagga
    aggacctttataccaatataccgcaataataatag
    aacattggtccccaagtgtatgtcaaccccaagtg
    tatagatttctttaaagattaaaatccctttttgttgcta
    aagcacctgatatatttttctatcaaactaaaaaaa
    ttgttagcgggatgaagatatattcgccaagaacc
    atagtgcttgtataacggcagaccattaattcaca
    actattattattttattgttagattgttgatagaatcgat
    tttgattgtggcagaatcgatcttgtaaaaactgcttt
    aaggtgcttacttataattaagaaagattcacttat
    gtaagttaagcatattaatcatatcattcggcctaat
    tcattaggaatattttgctattcgttttgccatcattaa
    caacaaaattgacacgttttcagccaaaagtatta
    acaactaaacctaaaacttcaaacattaaatagtt
    tttagtatctttagtttcaaactagtgatttgtcctaata
    tcaacactacgaacgaatttatatacattgaactttt
    ttctgaatcaccgattacaaaacgaatataatttgg
    tatcggcagttgctattaatttgatcggtttggactttg
    gactaatcacgatcaaatcttaaatggaccgaag
    tgaataaatccctaatgttttcaagagagtcacac
    gaacgaaacaaaggtaaaatatgaacatagag
    cgtggggaccttgaagcagaaggtctgtatggtg
    acagaccggtgagtggagtgtatgaatgaacga
    gaagtgagaagacaaaatacaagaaagagcg
    ttgacttggaagttaaagccaaaaaaaccacaa
    ggggcaaatttgtctctttaggaaaaggacacag
    acagactttctatacgggccaattagaaaaatag
    gccctacttctaattaaagcccatttacttctctcctt
    gtcttcttattcctcttttctccccatcacgtgacgac
    gatgctataaacgccgtcggattatataactggtg
    ccgttgacaagacggcgacagaagaaagaaa
    gaagaaaccacaggctctagggaacgtaacgtt
    atgtcctgtctatagcatttataacggtcagatcaa
    cgccgtttagataaagatctgtcaatgttaaagaa
    gagatgcatctctacaccgttaaatttaaaacgcc
    gtgaacctcttatctattgatttttgtttgatgaagcca
    aaacaaatcgtgtcagaagacttatcagagaag
    aagaaaacgacgacgttcccgtttctccatgtcta
    ataagtgtagtagtggcggctactaaaaactctaa
    agtttgactccagtaaaactgcctttctagtgtaatt
    ccagtgattttagagtttgaatagtgtgtgaccaaa
    tttgaaagtacaatctcagcaatattattgatcactc
    gttataaaagaatcgaatgtaaaaatagccaatg
    agagactgagacgtatgtgtttgaccataagtcgt
    atagtttgtatctatctacctgcaagatcagcagat
    ggttctctgatcaattgtaccttaattatcttttattttcg
    taaaatttctctattcacaaatgataaatctacttaa
    gacagtaaccataacaagatttacaagataatttg
    aaaaatgaacacataaaagtattttggcgcattat
    ttttaataataacaatatttatgtaaagtcacataaa
    agtatatattcgctcacaaagtcttacggtatttaga
    acagtagtaccacatcgattctcttcatcttcttcttc
    ataatatgccattgttcatgtctctgtgtcctatcgca
    taacactcacgctatcttattattttctctcgctctttct
    cactgagaggacactaaaaaa
 

Example 7

Nematode-Inducible Expression of the Potato CRN and CLV2 Promoters

[0155] The promoter for the Solanum tuberosum CRN and CLV2 genes was operably linked to a beta-glucuronidase gene (GUS) and introduced into transgenic Arabidopsis plants. The transgenic plants were then infected with BCN and expression of the GUS observed. It was determined that the StCRN and StCLV2 promoters can provide for expression in the root vasculature uninfected plants as well as upregulation of expression at sites of BCN infection in plant roots (FIGS. 17 and 18, respectively). The sequence of these and other nematode inducible potato promoters that can be used in the methods of this invention are provided in Table 5.
[0156] 
[00007] [TABLE-US-00007]
  TABLE 5
 
  Promoter   Sequence
 
Solanum tuberosum   CTCCGAGCGTGAAATGAAATTAATTCCTTTAGGAGAACATAAATGTCTGCA
  >StACR4 (clone 3)   GAACTATTCTTGAAACCTGGCGCAGAGGATAAAATAAATATTCAATCTATC
  promoter sequence; (SEQ   TAATAATAGTTGTTCACTCGCGCATTTCTTATGAAACTATAAATAGAATGA
  ID NO: 15)   TAATTTACTATATCACCTTTTGAATATACTCTCTCTGTCCCTAATTACTTG
    TCCATTTTGATAAATCAAGAAAGAACAATTTTTTTTTTATCTATTATACCC
    TCAATAAATTACTTTGAAACTGTAGAGCTTCTTGAAAATCTCAAGTTTTTA
    ATTTATCCACTTCATAATTAATAGGGGTAAAATGGTAAACTACTATGCCAA
    TAATTGTTTTCTTAATATGTGTGTCAATTCAAAAGTGGACAAATAATTAGG
    GACATAGAAAGTAAGAGATACAATATCTTGAAAAATGTAATAGGGAAATAA
    CTATAATTAATGATGAGTAAATTATGAACTAAGTGTAAAATTATTTATTGA
    TGTCATAAAGTAGACAAATACTCTCTCTGTCCAATAATAGTTGTCCACTAT
    TGACCTGACACACCCCTTAAAAAATAATAAATATTGTAATACTACTTTATT
    ATCCTTTGACTTTATTAAATTTAATGTTTTGAAAAATGTTTTAGATGATAA
    ATAATACCCTCTATCCCTAATTACTTGTCCACATTTTCTTTTTTAATTGTC
    CCTAATTACTTGTTCATTTTAATAAATTAAGAAAGGACAATTTTTTTTTAC
    CTATTATACCCTCAATTAATTATTTAAAAAAAAGTAGAACTTCTTGAAACT
    ATTAAGTTTTTTAATTCATCCACTTCATAATTAATATGGGCAAAATGGTAA
    ACTCATTATGTCAATTATTGATTTCTTAATAGGTGTGTCAATTCAAAAGTG
    AACAAATAATTAGGAACAAAGAGAGTATTTAATAGCAAGAGTAAAACAAAC
    ACAAAAGGTAAATTATATCTCTTAATTTTCTAGATTGGACAAATATTGATG
    GACAACTATTTTTAGTATAGTGGATAACTATTGTTAGACAAATAAAGTATT
    GTTGAATATCCCAAAATAATATAATGGACAACTATAATTAGGCGGAGGGAG
    AATTATTGTTGGACGGAGGAAGTAGAAACAAAATTTTTAAAGCTAGCAATT
    TTAGGATGATTAGGGGGGATTATGATAATGATTGTACTAAGTAGGTACAAT
    TATAATGGAAATTTTAGTTAATTATGGTGTACTCTGTAAGAAGAGAGAAAA
    TTTGAATAAAATTAAGTAGTAGTTATTTGTAGAAAGTAAGGGAGGACATGT
    GTGCAGGTATCCAGGCATTGAAATATCAATTTTGCAATAAATTTTTCATTA
    AATGCTTTCACCTACACTGCTCTTATTTTGAGAAGATGTAGTTTTGAAGCA
    TTTAATGCTCACTTTTCTCTCTCTATTTCTCCTATGCTGTCTTTCACCACT
    TCATTCTTGAGGGCACCGATAACTTTGACACAAAAGGGGCTAAAAAAAATG
    TCATTATGTTCTCTTTTTTGTGTTTCTTGAACTGAAATATGCAGCTTCTTG
    GCTACAAATTTTGTTTAATTGTATTGATAACGAGGGTATTATAATTATTTG
    GGAGGAAGGAAAGTTGTGAATTTTGATCTCATCTACCCACCCATGGTATGT
    TTGAACTATTTTTTTTCTCGTGTGTTTCATAAATTAAGTCAGCTACTATGG
    AGAAGGAGGAGTGGTATTTTGGTTCTATCAAAAAGGATAAAGGTGAAAGAA
    AGCACTGACTTTCTGTTTGTGTACTTTGTTTAATTTTTAATTTGTGTAATG
    GACGTGTTTAATAAGTGGTGTGTGGTGATGTGGAAATGTAGATACTTTGTA
    AGAGCTTTTATGCTTCTTGTAGTATTTTCAAAAGTATCAGGGTTTGGATCA
    ATG
 
Solanum tuberosum   CGATGAATAATGCTCCCTATCAATATTTTTTTTATACTGAGAATCCAAAAA
  >StCLV1 (clone   CAATTATAATGACCATGCTGAATTTCAGAAAATGGTGGGACAGTTATGAAA
  5)promoter sequence;   AAGATGTTGAGATTGGTTGGATCGGAAGAAGAGAACAAAAAGAGTGGACTT
  (SEQ ID NO: 16)   TTTACGCAATGCCAAAATTACAGAGTGTCTCTTACCCAAGGACACATACAG
    ACTTTGCCAATGGGCCCCAGAACCACCCATTAACCCCCCCCCCCACAAAAT
    ATGGGCCTTCCTACCATACCAAAGAAAAAAAAGAAAAAAAATTACGAAATA
    ATTATAAGATCGATAATGTTATATGATAATGAATATTGGAGCGTAAAACTC
    TAATAATTCATAATGGGAGGTTTGAAAGCAAAAATGAGTCTAATAATATAG
    AAATACACACAAGATAGATGCGCAGAGATTCGACTGTTAAAATAATCATGT
    GGTGAAATTATATACTAGATAAAATTAAAAATGACTAAATACATCAGTATA
    TCAATCGTTGCATTGATCAATAGATACACTAATTATCATAGCTATAAAATT
    ATAATAAGTAAAATACTGTAACAAAATAGCTCCTTCAAATCATATAAAAAT
    CTACACTAACACAATAAATAGATTTAAAAAAAAATATAGCCTAAACAACAA
    ATACCACACTCTAAATATGAGTTACGACCATTTTTTTTTTTATGGTTTTGT
    AAGGAATTAGGGTGCAAATCATTAAAAACGAAGATAAGATGTAAGTAACCA
    AAACGTGCATGCATTAGGATGCAAATCACAAACTACTCAAATTTACTATTA
    GAAGTGCTCATTTTAATAAATTTAGAGGACCAAAGTGACAAAGAGTTATAC
    TTAATAAACTAGTTTGAAATAACCCAAAGATAAAGATATTATTTTTGTTAT
    TTTCTAGTATAAATCTTAGCTGACAGACTCAGAAGCGTCAATCATCAAAAG
    AAGTTCACAAAAAGCCGTCAGTAATTTACTCTGTTTCTCATCTAACCATTG
    CTTCTAAAGTCTGCTGCTACAATCATTTTACTTGCATCTATACATATCACC
    ATGACTTTTTTTACCAATAAATATGAGATTTAACCGTAAGTTATTGAGTTC
    GATTGAATCTTCACGTAACATAGTAATTAAATATGAAATTATACCATGATT
    TAAAGCTAATTAAATATGAGATGGAATTATCGAAAATTATGATGAAGTAGT
    CAATACTTTTTCATCTTAACAAGAGATTTTGAATTTAAATTTGAATTTTGG
    ATGAAGTTACTTTTGATAAATAATGTTTTAACCTTAAACTAGAAAAGGTTG
    ATTCGTTCATTAAAATATATTCTTTCAATCTCAATTTATGTAACACTGTTT
    GACCTAATATAGATTTTAAGTAAGATAGAAAGGAAATTTTTATAAATTTAT
    GATCTAACTCCTCATTTTGGTGATTATAAATCATTTGATTAGTAAAGAGTT
    TTTGAAGTTACTCTTTTAAAATATTATAAAAATGATAATTTTTAATAAAAT
    AAAAGAAAATTATATATTTTGTTGGGTCTTTTAGTATCCAATATCCATATT
    TAAACTCGATTAGTTCTAAATTAGCGCTGAAAAGTGTTACAGTAGTTGTAC
    AAAATTCTCTAATAAAAGTGATTCCGTATCGTATTTAAATTTGAAAGCTTT
    GATTATGAATGATTAAGAATGGAGGAACAAAATTTGTTACCTTATTATTAT
    TTGGTAGAGATGAAGTATTTACCACTCCCTGTGGTATCTTCACTTTGTTTC
    CTNACACACATATATTCAAAGCCAAAAAGTTAATTTTGATTCTCCTTCCAC
    TTTGGCCAAATGCAACAGTACTAAATACTCAACACTTCAAATACCCTTGAA
    CCTATCCCAAAATTTGTACAAACCAGACTAAACTAACAGTGTAATA
 
Solanum tuberosum   CTCACTCGCCTCTCTCATCCCTCTCACCTTTTTCCTCCCTTTCCCATTCTC
  >StCLV2 (clone 7)   ACTCGCCAGATATACAAATACATATGTATACTAGTTACATACAGAATGATA
  promoter sequence; (SEQ   TACATACACAATTCAACAAATATACAAATTCAATTTACCTCTCTTCACTCT
  ID NO: 17)   ATGTCCTCTCTCCTCCCTCTCCCAATCGCTCTCGTCTCTTTCCTCCCTTTA
    AAATATAGCTACAAATCGTAATTATCAAATTATAGCTATGAAGCCTAATTA
    AGTTATTTTTAATGGTTATTTGTGAAATTTCCTCTTTTTTAAAATAGTTTT
    TAGAAAATCAAACTTCAGTAACTTTTAAGTTAAAAAATAAAAAGTAAGAGT
    ACCTACTTTTAACTTTTTAAAAATCGTTTTTAAAATATTGAAATATTCTTG
    GCGACTAAAAACTACTTTTAACCTAAGCGAAACACCCTCTGAATCTTAGTA
    GAGTAAGTTCTCGAGTCATATCATGATTAATTTATTTTCACTCGTGTACTT
    TAGCTTTTCATTTTTCCTTAATTTTGTTTTACACTACTATAAAATAGTGGG
    ATGCATCTATATCTTATCGTTTTTTTATGTTACATTAATTCATCACTTTTA
    AAATAATAAAAGTATTTAGATATATAGTTTTTGCCAAAGTTTTATGATATT
    ATAAAACAAATTTGAAAATCAATCGAATCGAACTGACACTTAAATAATCGT
    GATAATATTTAAATATTATAAAATAGAATAACTATAAAATTAATATTATGT
    AAATTTAATAAAATAATCGATTGAAGCGTACCATTGAAAACTCGAAAGTGA
    AAGGAAGAAGAGCATAATTGTTGATATGGGTTCACACGCTCACTTACATAC
    ATATAATAAAGGCTCTCTTTAAAGAGAATTTGAAAAAGAAAAGAAAAGTGA
    AGTTGTCTACTTTACTTTAGTTTTACACTTCTCCAGGCACGCCAAACACCT
    TTTGCCTCTCTTTTTTTTTTTTTTTTTCATTGGGACTGTTTTTTTTTTTTA
    GTTTGTTTTTCTTTTCTTTCATCAAAGAGGTATTTTTCGTTTCTATAATAT
    TGGGAGTAGCAAAAATGCTACTAGTATATGAAATGGCAATTAGTACTTTTA
    TTTATCATCAAATGATATATGGTGCAGTGTATACAATATTCAAATTCCGAA
    TATGAAAAAATTCATAATAGAAAGTACTTTTCCATAAGAGATCATACAATG
    AGAAAATATTCAAATTAATCAAACTCCAATACAGATACTATCAAAAATCAA
    ATGAATGAGAAAATAATAAAAAAATATTACCCCCATTTGATCACTCAACTT
    TTCTCTCCATTATTTAATACAAAAATATCAGGTGATTTTTCATATTTGTTC
    TAACTTTAGTATATAGAGTTATCTAGTACTCCCTTCAATTACTTTTGATAC
    TAATGCAACTAGGCTTGTCAATAAAATATTTCATTAGCTATTACTGATGAG
    AGATAACAAATATTTCATAAAATTAGTTGAAGTGCGCAAAAGACCAACCTC
    AAACACACAATCATAAAAAAAAAAGTGAGGAAATATAGAGTGTGTGCCTCT
    CAATAAAATAAGTACTAAAAAAGAAAACAAGAAACAAGAAAGAATGTTGGT
    TCTTTAGTGGTGACTCTCAATGAAGTACCTACTTTCAGCTTACTCTCTCTA
    TACTCACTACTACTGCTACTCAGTACTGATTCCTTTCACACATACTGTGCC
    TGTAAACCCTGTCCAGGGACCCCCATTTCCCCTTTCCCCTTTCCCCTTTCT
    CCTTCCTTCCTTAGCTATCTCTCACACAAACACTAATCTTTTTTCACCTCT
    ACCTTACCTCC
 
  >StCRN promoter   TCCGTTTGAGGGATTTCTGTAATTATAAACTTTTAAGGGATAGATTGTAAT
  sequence; (SEQ ID NO: 18   TTTGCCTTCAAAATATGTGATTTCTGTAATTTGCCTTATTATATAAACAAT
    GTGTATTATCCGCATAATTACCACTTATAGTAATTGAATAGGTTTTACCAT
    CTATAACATAACTTTTTTAACAATTTGTCTCCCTCTCCCATTCACTCCCCC
    CCCCCCCCCCCCTCTCTCCTTCTTCTCTTTTCTTTCTGCCCGTCTCTCTCT
    ATAAATTTCAATTATCCTAATTTAAGACTTGATTTTGGATCGAGTATTATT
    TCCAAACAATTGAGAACATCTTTGAAATTTATATCTTAATGTTTAAGGATT
    GTTGATGAAGACTAATATTTATATAACTAATATATTTTTATTACAATAATA
    AATCTAACAATGTTTAAAAGAGAAAAAATAATAAATAATTATCAGTATATT
    ATACATATTTATGTTTCAATGCACATGGTGAATATAATTAAAATATTTTTA
    AAACAAATGTATTATAAGTATTATGTGTGAATCTCAAACATTTCAGTACTA
    TTTAAATTAGTTTACATTGTTAGAAATGTATTATATTTGTTGGAATAACAA
    CAATCAAATTCATAACAATGTATAATATTGAATTTGAATGGTATTACAAGT
    GTCTTCTATATTTAATACAATTGTAATACATAAACTAATTTGAATAACATT
    TGAATATTTTAAATACAAGTACAATGCATTTTTAACACCATTAATAAGATA
    GAAAATAACAATTGTAAAACATTATGAATCCAATATATTATACTTATTAGA
    GGCATATTTCAAAACACGTGGTGAATATATAACTAAAACATCTTTAATAAA
    AATGTATTATAAGAATTATATGTGAACTTCAAACATTCTACTACCATTTAA
    ATTAGTTTACATTGTTAAAACTATATAGAGGTGGCAAATAGTTGGATTTGG
    ATGGGTTTAAAATGATTTAAATAAAAATGGGTAATTATCCAATCCGTCCAT
    ATTCTATATGGGTAAATATGGCTTGGATAATTAATGGACAGATTGGATATG
    AGTTACCCATATTTCATCCACATTGATTGAAGAAATAAAAAATGAATTTAT
    ATTTTTTAAGTTTCTAAAGTAATTTTTTATTCTACTCACTCCCATCCCTAC
    CTCCAATTCACCCACCCCTAATTTTAGTTTGTTTTATTTTTTCTAACCCCC
    GCCCAGTTTTTATCCCCCTCCACCTCACCCGTCTTCATCCCCTCTACCCCC
    ACCTGCACCCACCCACCCCCACTTTTTTTTAAAAAAAAATTCTACGCCCCC
    CTCCCTCAAGAATTTCCAATTTTTTTTTTGTTCTTCCATTAAAAAAATGAG
    TTTCTTTTTAAAAATAAAAATTTACCCCCTCCGCTCCACTCCTATTTTTTT
    TTTTTTTTGGTTTTTTTAAAAAAAGTAACATTTTCAGAAAAGAAAGTTACC
    CCTGTTATAAACTAAAGTATAACAACTTACTCTTGCTTCTTTCTTTGTTAC
    AAGAGGGGTATATATAGTTGTATACACTTGTGCCCAAAGTGTGATACACGG
    ATAACTTCTTGCCATGTATACACTTTGGACACCAAGTATATCAAATGGCTA
    ATTAGTATACACCACATAGCATTTTGTGTGTGTATTAATCTTACAACACTT
    AACATATTAGTGTGGACATTCAATTTACAACAACCCTGAATTGTATTACAA
    CTATCATTTATATTTCATACAACTTTTCAAAGTTGTAGCTCTTCTTTTCCG
    ATGATTCACAATCACCGGATTATCAGTAGCTCAAATCAATCCCATTATTAC
    AAATCACCACACAGTCCACCCACAGTCACCAAACTCTCTTTTCCCCATATT
    TTTGGTCCAAACACCATGACCAAATTTGAATGCCGAAGAGAGTTTTTCAAT
    TGGATCTAAACATCGATTTTCATGAAGCTCATCGGAGCAACGAACACCATC
    AAAATTATGTTCAGATCTAACAACACCACTGATTTATGTTCTACTCTTCTA
    CTTAAACAATGAACATAGAAACTACAATCTTCTTTGGTTCTTATAATTACA
    AATTAAAAAACAATAACGTAAAAGAAAAAAGATGCATAGAGATTGGGCATC
    GCATGGTTTCATGGAGCTCCATGTTTTTTTGTTGGAATTTGATGATTTTCC
    AATTTGGTTATTATGTTGTTCATTGTTGTTGTTGAGTCTATTTTGTGGTGG
    TGCGGAGGTGAGAGCTTTAAATTGGAGTTGGGGTGATTGTTGTTTTGTTCG
    CCGGAGAAGCCATCTCCAGTGAGGTTGGTTGGAGAAGGAGAGAGATGAGGA
    GAGCAATGAGTAATTTCAACTATTAAAGGTAAATTGAATTAATATCTCATA
    CGATCACTCGACTTTAAATAGTTTATTTAGAAAGTCACTTAACTTTGAATT
    GTTCACTAAAAAAATCACTCAACCTTATTTTATAACTCAAAAGTCACTCAA
    CTATTGATGTTTTACTTAAAAAGTCACCTAAGTATTGATATATTGCTTAGA
    AAGTCACTCAATCAATTTAAATAATTTTCCATTAAATTTTATTGTAAACTA
    TTTTTTAAAGAAATAATAAGATTTCTATTTTAATTATCTTATTAATCCGCT
    CCAATTATTTAATTATAATTTTCTGAAAAAACGTATAGCAATTGACCCAAA
    AAAAAAACTTTTCCGTTCTAGTAGTTGTTTGATTGGAATTAAATATGTTTA
    AAAATTATCAAAAAAAAATAGGATGTTGGAATTGATAAGAAGTAATAAAAA
    AACGCACAGTAGCAATCTTTTACTATTTTAAAAAAAAAAATAGTTAAAAAC
    AAAAACTACATTTCAACGAAATTCAATAAAATAATTTAAATAATCATCTTA
    TTATTTTTTAAAAACTAGTTTAATTATTTTTTGTATTTTTACAAATGAAAA
    AATTATTTAAATTGATTGAGTGACTTTCTAAGTGAAACACTAATAACTGAG
    TGAATTTTGAGTTATAAAAAAATTGAGTGACTTTCTAAGTGAACAACTCAA
    AATTGAGTGATTTTTTAAGTGAACTATTCAAAATTGAATTACCATATGATA
    TATTAACTCAAGGTAAATTAGGCTATGGACTATAATAGAAAAAAACCCAAA
    AAGGATAATAATTAATCTAAAAGAATTCATATATATATAAAACTATTTTGT
    TTAATGATAAATTTTTGACCCATTGGGTCTTTAAAAAAAAAAGAGAATACT
    CCATCTTGTTATTTTGTAGGTATAAAAAAAAAGTAGTTCTATCTTTAATAG
    GTTCATTTCTTTAGTGGAGGAAAAAAGTGGATTTATTCACTAATCTTGTTT
    TGTGAGAGGCAAAGTTGTTACATATTTGGAATTTGAACTTTGTAATGATTC
    TATTCTTGTTCATTGTGAAGTTGTATATATTCCTCACTGTTCACTTTTATC
    TTATTTTATTATTTATATAATTTTAAAATTAGCTTTTTCAGCAAAAGATTT
    TTGTTCTTGAAGATTCGTTTCAGAAAGAGAAAAAAAGAAGAAAATGGTCAC
    ATTGTCGTCCTTGTGTAACATTCAGAGGAGTGAACCCTAAACTTGCCGACC
    CACAGAGAAAAACAACCCTAGTTTCC
 
  >StBAM1 (clone 6)   GAAGGGCATAATTGCTACTTGGACAACACAGTATAATTAATAGGACAACGA
  promoter sequence; (SEQ   AAACTTCGTTTCATAAACTCATTCTCTAGCTTAAGTATAATTAATATGCCC
  ID NO: 19   CTAAACTATTTGAAAAGGTCTAGATATACCCTCCGTTTAAAAGTTTGGCTC
    ACTCATGCCCTCGCCGTTCAACTTTTTGTCTAAATATGCCCTTATGGGCAT
    TAGTTGGCCTGCTGGACATATCTAGCTCATTTTCCATTTCTTTAAATGCCA
    CATGGAATTGTCATGTCATTTTGACTTTACCACATGACATTTATATGAAAA
    TGGAAAGGGATCAATTATGCCCGTAAAAAATTCGAACCCATAAACACCTAA
    TCCGACCCATAAATCAACCCCCCCTCCTTTTAGATAAACTACCCGACCCAT
    TTTCAATAATTTTGTTTAAATTTTTATTTTTTTCGGTAAATCCAGGAAATT
    AGTAATTGATTAATAAAAAATAGAAAAAATATGGGGAAAAAAAATTAACGC
    CAAAAATTCACAAATAAATATTGTAACCTTAAATTCAACAATTTTTTTATT
    TTTTTCCGGTAAATCCCGAAAATGAGTAATTGATTAAAAAAATATATGAAA
    AATATAAAATTAACGCCAAAAAATCACAAAAAAATCCATTTTTCATATAAA
    TGTCATGTGGTAAAGTCAAAATGACATGGCAATTCCATGTGACATTTAAAG
    AAATGAAAAATGAGTTGGATATGTCCAGCAGACCAACTAACTCCCATAAGG
    GCATATTTAGACCAAAAGTTGGACGACGAGGACATGAGTGAGCCAAACTTT
    AAACGGAGGATATATCTTAGACCTTTTCAAATAGTTTAGGAACATAATTGA
    CCCTTTACCCATTGCACAAAATATCATTCATTTTGAAAGTAAAAGCAAATC
    AAAATGACATGGAATTGGAATAGCACTTAAATGATACTCCCTCCTATCCAT
    TTTAGTTGTCACTGTTTACTAAAAATAACTTGTCAAAAATATTGTCATAGA
    AAACTATGAATACATACACATTATGTTATGATTGTTTAGATTGGCAGATCA
    GTCTTGTTTTTATATACATTTCTTTATGTTCAACTTGAGCTAAAGGTATCA
    GAAACGATATTTTTATTTTTTCAATGTAGGAGTAAATAAGAGTTTATTTTC
    TTTGTCTCATATTAATCATTTTTATTTTTACACGCATATTAACAAATCATA
    CGAAGATAATTTTACTAATTCACTTCTTAAAAACTTATTGAAATTTTAAAA
    ATAAATGTGAACACTTTAATTTTTTTTTTGCAAGGGTAACAATATAAGAAA
    ATTTTAATTAATGTTTTCTTGATTTAGTAAAATGGACAACTAATATAAGAC
    AATTATTTTTAGTAAAATGATCAACTAATATGAGACGGAGAAAGTAATATA
    TAAAATGTCATTCTTATTAATAATTTCTTAAGGAATGTGTAAAATAAAAAC
    ACGATAACTAATCTCTCCTCTATTGTGGCTTTCTTTGTGCCATACTCTACT
    GTCCAAAAAATATTACTACTCATCAAAAGAAGAAAGGGCTTTCCTTAAGAA
    TGACATCTTATCAACTACAAAACTAACCTAAAGATGAAAAAACTACAGACG
    TTAGTGGAGAATGTTTTAACACCCTAAATTAAAGGAGATAAAGATAAGTGA
    AGTGCTTTTTGTGACAAACGAATTGAATGGAATTTTATGCCTCCCTCCCAA
    ATACTCTTTTTAGCTAATGAAATCTCTTTAACTAGTAAGGACAACTATTCA
    ACACGAGAAAAAGCAAGACCAATAGTTGTTTTTTTCTACTCTACTTTTTAT
    CCGTGAAAAGATTGTGTAAATGTTAGCAACTTTATTATTTTTAAGGAACAA
    AAAAGTTGGTTCCCCACGTTACAAAAAGAGTTGGGGCCTCCTCTACTTATC
    TCACAATTCAAATTTATTCTTTATAATATAATAATCAATCCCCTCCTATTA
    TATATATTTATTTACTCAAAACAAAAGAATATACACCAAACGGATTACCCA
    CCCCCTCCTCACTTTTGCCTTTCTCACTCTCACTGAGTGAAACCGCAAACC
    AAACAGTTGGTGGGCATTAGATTAAGGAAGGAAAA
 
  >StBAM2 (clone 2)   GCGTCAAAGTATGAAGCAGACAACACATGAACACACAATAATGATCGACTC
  promoter sequence; (SEQ   CCACTTAAAAATATTATTATTTTTTTGTTAAAAGGGAACGAAAGCATTATT
  ID NO: 20   TTTATTCGTTCACTATTTTAAAATTAATTCTTATTTGTACTTATCACTTTT
    TAATATATTAAAAGAACTTTACTTTTAACATCAATTAAAATGATATTATGA
    TAAAACATTCCTAATCAAATGTTATTTCTTAAATATGTACAAAGTTTAAAG
    TGGATCAGTAAAAATGTTAATGAAGGTAGTAACTTTTATTTGTTGTTTATT
    TACTTTGTTGATGTGTTTGTAATTTATAATCTTAAAGAATAATTATTAGAA
    TAAAATGAAGAAAAAATAATTAATTCTATTTTAAATTAACAAATAATTTAT
    AGTAATTATTTTTAAAAATGACGATAAATAATTTAAAACGGAGGAAGTATT
    AACTGTATTAATAATTAATATTAATACCACTAATGATAATGAAAGTGTTAG
    TATCCTACATGAAAAGGACATGATTGACTACTTTCGTATAATTTGACAATG
    AATTGAATGGAATATTATTTTTTTCTACATATTTGTTTTTGTTGTTAATAA
    TGTCTTAAATTATTAAACAGTTATATAATGCTGAAAAGAGAAAAACAAAAA
    GTATTGAATTCTCCTCTTTCTTCTCTTCCACAAAAATTGNAAAAAAAAAAA
    GCAGCTCTTTTATTAATATATATATTTTTTCTTTATTTCAAGTATAAAGTT
    TATTTAATGAAAAAAAATACTTTTAAAATTTATTATTTTAAATATATCATA
    ATATTTATGTTACTATTAAAATATTTATTATGAAAATTAAATTAATTTCAA
    ATACATAAATGTATCATTCTTTTCAAATATCTTTTGACTATGGAAAGAAAT
    TGTAAAGTAAACGATGACTTTTTTATTTTTTTGGTACTTAATTGATTTTTG
    AGGAACAAAATAATTGTCCCAAAGTATAAAAATAAAAAAAGTTGGGACCTT
    TTCTCTAGTCTCCATATGAAAAAGACAATTCAGTACTCAGTAGATTCAAAA
    TATCCTTTAAAAGCTAGAGCTCTTTAATATACAATAAGAAACAAAATAATC
    ACAAGACGATAATTATTTCAATTTTAAATGTAAAATTTTAAAAAATATACA
    AGTTCTTTTTAAGGTTTCACTCATAGAGCTGTAAACATATTTTTAAGTCCA
    CATACAACTTCTAACTTCTAAATATTCANTTTCAATCTAACTTCAAACACT
    ACANTTTTTCAATAATAATCAATTTATGTCCGACGCTTATTTTGTTGATAA
    TTAGGATAGAATATTACTAGTAGATAGTTGAGTGTTATCACATTTTACGTG
    AATGTGAANNAGAGAGTGAGCTGACCTTCTTCTATCCTCTTGTTTTTTTAA
    GTAGTATTATTTAGTTATCACGTAGTTTCTTACCTTCCACGTATATTGTTA
    CCTATTGTTGTATTTATTTATTATCTTGCCATTTTGTTGTTTCTTTTCAAA
    TAATTTTACACGACGTGTGATAAGTGTTTTCCTTTTGAGTCAATGGCCTTT
    CAAAAACAATCGTTTTTACTTTATAATCGTGAGATTACATTCAATGTGTTA
    TCATTACATTGGATATGTTTAACATTACATAAGGATGAAGAACGAATCAAT
    CTATTCAAATATTAAATATTCATTAAAACAATACAATACGATATAACCATC
    CAAACCAAACAGAGTGTCAATTTTTTTTAAAATTATTTTAGTTTCTAATGT
    ATATATTCAAAAATTTCATATAAATACACATTTATAATATATCTGTTCGAT
    AAAGACACGTGAACATTTCTTCTTCTTCTCCACCATTTCTGCTCTGCTCAC
    TCTTTCCCCTCCACCATTGAAGAAAC
 
  >StER(clone 2) promoter   CCGAACATCTTTAGGGCATCTCCAACCGAATCCTCTATTTTACTCTTCAAA
  sequence; (SEQ ID   TATAGAGTTTTCTATTTTTTTCAGACAACCAACTCCAACTCAATTCTCTAT
  NO: 21)   TTTACTCTCTAAAAATGAATTTTTTTTTCTCTCCTCGATATTATATTATTA
    TTTCTATTTTATTCTTATTTTCTTATTTCATGATATAAATCCTTTATTTAT
    TTTTTTCCAAATAATTACTTTATATAATTTTTAATGTGATATGAAATTATA
    TTTTATTCTAAAATTTTAAATAACATAAATTGCAGGAAAATATAATATAAT
    ACATAAATTAGGGGACAAATTCAAATAAAAGTGATATACAATTACATAAAT
    ACTCAATTTTTAAAATTATTACGTTGCTCCCATAAATGCTCTATTAATGCA
    TTACGGAGTTCAAAATGAACATTTTTGTCCTTAATTTTTTTATGTCTAGCT
    AAAAATTGTTCAAACCGAAGATTTTCACTAGCTAAAAATTATTCAAATCGG
    GGATTTTCATCTACCATCATTTCTATAGTTGGAGTTGGAGCCTCTACGGCA
    TCTTGAATTGGTGCATTGAGATCACATTCATTCTCAATTTTCATGTTGTGC
    AGTATAATACATGTAGTCATTATATCATGTAGCACCACTTCTTTTCTCCAA
    AAATGTGACGGTCCTGCAATAATTGCAAAACGTGATTGCAAAAAGTTCGAG
    GACAAGGCTCTACTTTGCAATCATCGGAGTCCAAGACGAAACTAAAATTTT
    AACGAAAAATTTAGAAACTATTAGTGATCCAAATGTTCGTGGTTACCTGCA
    ACGAGAACAACAACGAATACTTGAAAAAAGAAATCGACAATCACAACCGCA
    ATCACAACCATAATCGCAACAATTCTCAGAATCATATCCTAATTTTTTTCC
    GAATAGTGCTAAATTTGAAAACGACCTACCGAATTTCTAAATTATTGTTGT
    GATCAATTAATTATTATGTCATGTATTGTATTTTATCTTGTATTTAAATTA
    TTATGTTATGTATTATATTGTATTGTTATCTTGTATTTAAATTATTATGTT
    ATGTATTATATTGTATTGTTATCTTGTATTTAAATTACCATATCATGTATT
    GTATTTTTAAATTAATTTTTTTTGCGTATCCTTTATAATGAAAATTAATAA
    TAAAATAATTTTATTATTCACGAAAATTAGAAAAAAAGTTAAAATACTATT
    AATTTGAAATTAAAATAGTATATATTAAATAATTTTTTTAAAAAATATTAT
    ATTACATTTAAAAAAGAATTATGAATATTAGATATTTAATTAATGGAATTA
    TATGTAAAATAATATGTTAATTAGAAAGTAATAGAAATAATAATAAAATAA
    TGAAAAAGTAGAAATAAAGAGCGTGAATAGTAGAATTTGGAGAACTATTCA
    ACTCTCAAAATTTGAAAAATAGAGGGTGATTTGGAGGTGGGTTGGAGTGCC
    CATTCTCTATTTTACTCTCCAAATATAGAGAATGAAGAGTAAAATAGAGGT
    GGATTGGAGATGATCTTAGTGACATTTTTGATTCCGCCAATGCTCAGTTGG
    CGTAGTCGCTGTCAAACTTGAGAAAGGATTACCCCTTTAGGCTTGCACAGA
    CAGTGACTTATGATGAAATGAAGCCAGAGAAGGCACTCTGTTATCACACTT
    AAATGAAAATACATGTGTATGGACTAGCAATAAAAGGGGCACTAGTAATTT
    TAGTAATTGAAAAGCAAGTGTATAGAGAGAGATAATGAGAGAGAAAGAGTA
    AGTACACTACTACTGCTACTATCCCATATAGCTGTAATGTTGCAGGTCTGA
    TTTTTGCAGTTGCAGACCCCCTTCTTGGCACAAGCTCTTTTAACTTTTATC
    TTCTCAAATAATTCTCTCTCTCTCTCTCTCTCTTTTTTCTCTTTTTACATT
    GTGAGGAAAGCTGAACACCCCATTGTATGTATTAGTGTGAGGCCTATCTGC
    CACAAGGATGTGATGGAACACTATGCTTCCTCTGCTAAAACCCCCACAACC
    CCAAAACTCTTTTTCACTTCACATTTAATCACAATTCCTCAGTGAAATTAT
    TCTGTTGCTCTCTCTAATTTCAATTTCAATGTCGGTAAGTCCAAGACCTGG
    TTTTTCAATTCAAAGGAGCTGAGTTAGTGCAAACACTTGAGGTTTTGAGTT
    TTGACAGAGACTTGAGTCTCAGAGAAACTACC
 
  >StERL2 (clone 1)   CCTGGGAGAAAATGAAAGCATGATCTCTTTCTTGTAAATTGTTTCTACCAT
  promoter sequence; (SEQ   ATTTTTTTTGGCACGATAAATAAATTTATATAAAATTGTATGAGTGACACT
  ID NO: 22   AGATGACAAGTCACATAACATATATATTCAAATTGATTTGTATTATTTATA
    GAACGAAAGTCTACTGTTTAACCTTATATAAGTTACAATTTAGTTATGTAT
    ATAAGTTAAAATTAAATTAAAAGACATTTCGAAATAATATGATTATACCAT
    TTCGAAATTAATTAGAGAGAGAAATAAGATCTCGCAAAATTAAGTGTCTTC
    TTGAAATTAAGAACCATTTTTAGGAGATAATTATGTATTTTTTCATTTTTA
    ATTTGACACGTATGCATATCCACTATTTTGTTTTATTCCAAAGTGACCCCT
    ACTTCTTTTGGTAATTTCTTTGAGTATTTTAAACTCTAGTCCCCCTTTCTC
    AAGCAAAAAGGCTCACTCGCGCACGCGCGAAGAGACATTGTGACGCGCTGG
    ATGGAAAATCCAGAAGCGTAACTGTCAAAAAATAGAACAACTTTGGGAAAC
    GGGGTGACGGCCGCTGCCACCACTTTTTTCATTTCCAAACACTCATTAACT
    AACGTCGTTTCACCGCCGTTTACTGCTTAATGAGTATGAATTACACTCTAA
    TAGTCTATTTTTACTTATTTTTAATGTGTTTATCAAATTATATTTTTAAAT
    ATAATACTTTAAAAATATTATCATCAATAATAAGAGTAAATTAAAAAATAA
    ATGACAAATTGTTTCTTAAATTGTTAAATTAAACAATTAAAACTGAATATT
    TACAAAATACCTCTTAACTTGCTAAATTAAACAATTGAAACTATATTTATA
    TTAATAAATTGAACTGACAAAAATAAATAAAGGAACTATATATTTTCTCAA
    TTATATCTTTTTACTAAAATATTATTTTTCTAATACTAGTTAAACTTTTAA
    AAAACATCTAATAAAGAAAAAGAATTTGTTCAATTATACTTTAGAAGCTTT
    TATTATTATTATTATTATTAGTAGTAGTAGTAGTAGTAATAAATTAGATTA
    AATTAAAGAGAGAAGTATTCAAAACTCCCAAAACTATTGTATTAGTTTTAT
    TTCAGAACTATTGACAATCTTAATTTTTTTTTTTTTAATTTGACTAGGTGA
    ACTTAAATATACTTCATTTTTTGCAAAACAAGTGAAGTACACTCTTAAATT
    TTCATCAAGTTTAGAAATGTTTTCAACAATTTACTAGACTCTTTATTAAGA
    ACTTCATGTTCTTTCAAGAGTTTATGAGCACTTGCTATGTCATGTTACAGA
    TCAAGAATATCTACAGAGTGTATCTAAATTTAGTACTAGTAAAGTAGAAAA
    TGTATTACTTATCTCTCAAACAATAGGTATTCATTATACTATTTTGAGATG
    TCCAACAATTTTTTTTCACTTTATGAAATCAATGAATAATTTAACACTTAG
    TTCCTAATTCCCAGTAAGCATTAATTATAGTTATTTACTTATTATATTTTT
    CAACACATTATATTGAAAAAGTGATATAGTAAATCTATCTTTTTATTTTAT
    TATTTCTTAAAATTTGTACAAACTTAATAATAGACAAATATTGTTGAATAG
    GAATAATAATTTACATTAAATCCAATATATTTTTCAATAGTTGTCACTAAA
    TGAAAATACTTCATCTGTTTCAATTTATGTGATAGTTTTCATTTTTCAAAA
    GTCAGACAATTATATATTTATAAATTAAGTAAAAAATATTATAAGTCACAC
    TAATTAACAATTCGAAATATTCGGTACGGAGGAACTAACACTTATGTTTTT
    AGACCATATTAGTCTTTTCTCTCTATTTATTATATAATATTGAGAGGAGAG
    TGCAACCACCATGGCAACTTTCTCTGTCTTCATAAAACGCAGCTGACATTA
    AAAACACAGACACACACTTCGCATTTCATATCCCTCTCACTACACGCCAAA
    TGCCTGCTCTTCCTATTTCTCTTCTTCTTCTTTTTCTTCTTCTCTCTCATT
    CACATAACACACATTCTTGTACTAACTCTGCATCATAAACTCTACCCCACT
    TTCTTCTTCTTCTCCGGTCATATTGCTCTGAAACTCCACTTATTGCTCTCT
    CCCGGCATTTATTTTTAGTTTCTCAGAAATA
 

Example 8

Inhibition of Nematode Infection in Transgenic Potato Plants Expressing miRNA Directed Against the StCLV2 Gene

[0157] Transgenic potato plants that expressed an artificial miRNA (amiRNA) directed against the endogenous potato StCLV2 gene (SEQ ID NO:11) were generated and assayed for both expression of StCLV2 and for resistance to G. rostochiensis infection. Two independent transgenic potato lines tested exhibited both reductions in expression of the endogenous StCLV2 gene and reductions in the numbers of G. rostochiensis (FIGS. 19 A and B).

Example 9

Soybean Nematode CLE Receptor Genes

[0158] Sequences of various candidate soybean nematode CLE receptor genes are provided in Table 5. Inhibition of the expression of such genes is anticipated to be useful in the control of nematode infections in transgenic plants. It is further anticipated that promoter sequences associated with these soybean genes will be useful in providing nematode inducible expression of operably linked sequences. Start and stop codons are underlined in the genomic and cDNA sequences provided. The soybean PNCLEPRG promoters and 5′UT of Table 5 (SEQ ID NO: 23, 26, 29, 32, 35, 38, 41, 44, 47, and 50) thus comprise the nucleic acid sequences located 5′ to the start codon of those genomic sequences.
[0159] 
[00008] [TABLE-US-00008]
  TABLE 5
 
  Soybean Genomic DNA sequence, cDNA sequences, and protein sequences
  Sequence  
  Description   DNA OR PROTEIN SEQUENCE
 
  Glyma09g29840   CAGTTCGAATCCAGGTTGCATGGAGATACAGGAAGAAACGTAAAAATTGTGTTGATACCTCAAAA
  gDNA and about   TTAGATCAATCATTTAACTCATAGGTTGTATAATCACCTGAATTGCTTGTAATTACCATGCACAA
  2.8 kb of promoter   TTCCTTTAAAAATTAAACAACAAGCAAATGTTACTGTTGGAGAGCAATTCAAATTTCAAAATAAA
  and 5′UT Sequence   TGGAACTTGTGAAAATTCAAGGAGATATTTTTAGGAATTTGTTATGTTAATTTCAAATCTTTAGA
  (SEQ ID   ATTTTATCTAGATTTAAATATTTTATTAATTTGTTTAACTTATTTTAGGGATTTGTTTCCTTTTT
  NO: 23); Soybean   TAAAAGATTAGAATATGATAATATTTAAATTTTGTATTGTTATTTAGCTTTATATATAGAGCCAA
  BAM1-like gene:   GAAATACAAATTTTATAATGTGTTCCATCTAAGATTTCTTGAACGTGTGATAATTTTGTTGTGTA
  ATG start codon and   GAAAATTTTTTCCAACGGTTAACATTTTATTAGTAGTGCTTTGCTTATAATGCAAAGAGCCTTCT
  TGA stop codon   CCTTTATTTTATGTCTACAATAAGTAATGAATTTATAAGGAATGAAAATAACTCTTAACTCTCAA
  underlined   GAGAGGAAAGAACTTTGGTAAACAAGATTTCATATGTTACAGCCAGACTTACACAGAATATTTCA
    TTTCACACAGCTCAGATGATTTTTAGAGAAAATGTACCCGATATATATTCTTCTTTTAAAGGCAG
    AGTTGAAATCTAAATTATATGAGCAAAATATACAACCTATACAGTATAGACAGAATCAGAAATAA
    AGTTCATATTTCTTAGATTACGGTATGAGAGTCACTGAGTCAATAACTTTTTACTACGAGAATAA
    AGAAATGGAATGATTGAATGAGCAAAATATACCCCTGAATTCCATTTTCCTAGAAAGAGAAATAG
    CATGCGATTGAATAAGAGAATGGCACCATCAAGATTGTGAATGAGAAAGAAGAAATGGAGGAAAC
    TTGTGAATGGAAAGAGAGTGAGAATGGGAGAGAGCATAGTGTTGGACAATGACATTGTGACTGTA
    AGGAAATTAATGAGTAACTAGAGAACGGAACGGAACTAACAAGCTTCTTGTTGTGTTTGTGATTT
    AAGTGTTTGATGGAGTTTTAAGGATTCAATACAATGAAAGCTACGTGACAGTTAAATATATGATA
    GATTCATCCTTTGAGTTCCAAGCAGTATACGTGAACGGAATCAACGTTGATCTTTAGGAAGATCA
    TTCTCTCCGCTCGGAAGATCTTTTATCGTTTAATCGAATCATTTTTTAAAATTTTCAGTTTTCAT
    TATCATGGTAAGTTTATTGATTTTTTATAATAATTTTTTTTGAAGTCATATAAAATATAATATTT
    TATTGATTAGAAATGTAAAATAATTTACAATAAGGGAAAATATTTATTGAACATTTTTATAATAT
    TAGAAATAGATTAAACTAATACAGTATTTCGGTATTGTATTGCATATATGTTTATCTATAACTAT
    TATTTTTAAATTATCTTTTAATATATATAACGATTTTTTTTTTATAAACTTTCAAAATGTAGATG
    TTACTATTTTTTCCTAAAACAATATTATCACTATTTTTTCATTTTTTTTCTTTTGAAAAAAAAAG
    AAAATAAAGATAAATATATGAAGTGTCTTTCTTTCAACTGGTCTTATGTAAGAACAAATTACACT
    CTATGCTCAGGACTTATTATACTTATACTTCCTACGTTAAAATGTATTTTTTTTATCTCTTCTAA
    AGTAAATTATCATCGTTTAACTTTTGAGAAAAATGTCAAAAAAAAATCCATACACTTAACTCTCA
    CAATCTGATTCTTCTCCATCTTTATTGGCCTCTTCTTTGTCATCCACCCTCCCGGTCAGCTAATT
    TTTTTGTTATAATATTATTAATATGAAATATTCATCAACTTTATCGATAAATAATTTTTATTAAA
    ATACTTAATTAAATATTTTTATGATGATATTTTTTTCTTTTAATTATATTTTTATTTTTCTTCAC
    AAGATTAAAATTTAATATCTTTCTTAACGAGATTAAATAAATATTTCATCAACATATTTTATTTT
    TATATATATATATTTTTTAACTCATCATATCACTTATCATATCTATATTTATTTTTATGTATCTT
    AATACATCATTTTAGATGGGCAAATTAAATATATTTATCCAAAAGTAATGTCATGAGAATGAGAA
    GAAAAGTTACATCACGCCTCCTTCTGGCCTTCTCCTAAATTATCGAGATTAATACCTTGTGCCTG
    TAAATTTGGTAACCAGAAAAAAGAAAAATCATGTGAGGTAGAGGATTTTTTCGAATGTGTAAAAA
    TAGATTTCTTGAGTGCCTAAGGTGTTTGCATTCAGCAATGGCACAACACGTGTCAAGTCCCAATC
    TTACAAGAACCTTCCTTCCTACCGAAAGTCCCGTCACGACACGTGAGCAGTCACATCCGTCACGT
    GTCACCTTTTCATCGACCATGGGAAGATCTTTCGGCACCGCACTTTCTGGTATCTTCACGCGCAA
    TCCCCATCCCACCGTCCATTCTCTCACACGCTCGAGCCATCGTAGCCGTCGCCCCCTCACCCGTC
    CCCAACTCCGCCACGCATCCAAATGACACGTGGCGCTAAAGTAACGGTCAAATCCACAATATTAC
    TTATTGTAACCTTATCCTCTCCTCACCCCTCACCCCCCCCCTTCCCCCTATAAATCCCCCTTTCC
    CTCCCTCCAATTTCAACCTCACTCTGCATTCGCTAAACCCAAAACACTATTTTATTATCTTCTTC
    GTCTGTTCTTTGCATTGAAGAAAATTTCTTTGAATTGAAGAAAACTTGAAATCGAATTGTGAAAC
  AGAAAATAAACCAAAGGAAATTTTTACTGATTGAATTGTAGAGATTGGAAAAATGGCGTTGAGTA
    TGACTCAACAGATCGGGACCCTAGCTGGTGCGACGGTGCCGGATTCCTCGGCCGGAGAATCGACC
    GCGGCGGTGAGTGCTGCCGCGGTGTGGAAGTCACCGACGGCGAGTCTGAAGTGCAAGGTAATGAG
    GACGGATGGCTGCGCGGAGGGGCTTTCGCCGCCGCTGAGTCCGTGCAGGTCGCCGGTGCTGCGGG
    CGGATCTGTCGGCGGCGTGTCAGGCATTCACGGCGGAGGTGGCGGAGGAGGAGTACGTTGCCGGA
    GGGAAGGAGGAGAAGGGGAAGGGGAAGGAGGGAGTGCCGGTGTTTGTGATGATGCCTTTGGACAG
    CGTGACGGCGGGGAACGCGGTGAACCGGAAAAAGGCGATGAACGCGGCGATGGCTGCGCTGAAGA
    GCGCGGGGGTGGAGGGGGTGATGATGGACGTGTGGTGGGGTTTGGTGGAGAGAGAGAAGCCTGGG
    GAGTATAATTGGGGAGGGTACGTGGAACTCATGGAAATGGCGAAGAAGCATGGCCTCAAGGTGCA
    GGCTGTTATGTCATTTCACCAATGTGGCGGTAACGTCGGAGACTCTTGCACGTGAGTCTTATGCA
    ATCCCTTCTTCTTCCTTCTTTTTTTCTTTTTATTTGTCATTTGTGATTTTTATTTTTACTGGCGA
    AATCTTATTAGATTCTAGATTAATTGGTTTTAACAATTAGAATTGTTACTAGTATTTTTTTTTAA
    GTTTAATTTCTGCGAATTGGTTTTGAAATCTGAAAACTAATTGAGTGACACCATGAAAAGATTTT
    ACGTTTTTGATACATTCTTGTTGGTTTTTTTTAACGTTAAGTTTTTGCTTTTAATTCAATTTACC
    ATGAAATTCACATCTTTATCTTTATTGGTAAATATGTGGTGTTATTATTATATGGTGTTTTCGTT
    GATTATGATTGAAAATGAGAGGCGTGCCCAGCACGGTGCAGCTCGTTTGTGAAAAATAAAATAAA
    CGTTTTAAAAGGGGTTTTGTGATGGGAAATGAAGCCATGCCATGTGATGTTGGACTTGTATCACT
    TTGATTCGAAGTATAGTATTTTTCTTTTCTATTGAATATTCAACTACGAACCTGGAATAATTGAA
    TCTTGAGAATTGTGTATATGATATTGATAATTATTTAGCCATTTCTCTTTAACTGAAATTTTAAT
    GTTTCATTTTTATTAGTACTTGAAGATTCTGAATTTAATTAAATTTTAATCCTTTTTTTACAGAA
    ATTAATTTTTAATCTTTGTACTATACAGAATGAGTTAACATTCTTTTATAATTAGGGATAATGAC
    AATTTTAATTTAGTATTTTAAACATGATGATTATATTTATTTTTATCATAATAACAACAATTTTC
    CTGAAAAAAAAATAAAAATAATTTCATAAATCTTTATATTATGATTTAAAGAGGCGTAATGAGCA
    CGGTGATGCTAGTCTTATTTTCTTTCATTTTTTGTGGTCCTTATGTAAAAAGTAAATACAAAATA
    CATGAGAAAAGAGTGTGCTTTCGTGATGGGAAGTGCCAAAGTGGGACCACGTGAGGATGGACTTC
    TAGTTCTACTGATTCACGTCGGCATCGCCACATACAGTAGACTAACTTTTAAGGACACCTTAAAT
    TTAGTGGACCCGATATCTTAATTTATTTTTCGGTCCATTTTTTGAAAAAGTATTCCTCAAATTCT
    CTCCATTTTTCTTAAAACATGTTATTCGAAACAAATAATCCAGGCATAGTTTCTGTTTATATATT
    TTATGTAAATTATTTTTGACAGTTATAAGATTATCTAATGGTTTCGAATTCGAATCATGGACATG
    TGGTAATGTTGATACTAAACAGTTGGAGGAGAGTTTAGCATCCATAATGATTCTATTCGGTTTCG
    AGTAGAATTATCTCTTATTAGAGATACATCTGATCTACTAAAAAATATAAATAGTTAGTGTAATT
    TTAGATATTACTGCCATTAATTTTGCTATAAGTTAGCACTGTGTTGGAATACCAGTTGTCTTATT
    GGTGGGCTTATCAGATAGTTTGTCCTGTGTTCAGTATTCCTTTGCCCAAATGGGTTGTGGAGGAG
    ATTGATAATGACCACGATCTTGCATATACTGATCAATGGGGAAGAAGAAACTATGAATATATATC
    ACTTGGATGTGATACTTTGCCGGTGCTCAAGGGACGATCCCCAGTTCAATGTTATGCTGATTTCA
    TGCGTGCTTTCAGAGACACTTTCAAGCACCTCCTTGGTGATACCATTGTGGTAAATATCATTCTC
    AGTGCACTTTTACATCATGCTGTGATTTGTTGTGCTATTTAAATATAACTTCTCATCTGAACTTC
    TTTTACTGGCAATATTTCAGGAAATCCAAGTTGGGATGGGACCAGCAGGTGAGTTGCGTTACCCT
    TCGTACCCAGAGCAAAATGGGACATGGAAATTCCCAGGAATTGGTGCTTTCCAATGCTATGACAA
    GGTATATATATTTATGTTTTTTTTTTCCTTCTCCTTGTTGTAGTCCTTTATATATAATTGTCTTA
    GGATTTGTTTGGATAAATAAATTTCTTCATGAACAAAGAGGAGAAAACAAGGTAAAATGTGTTCT
    AAACCTCTAATACTTAATTATGCTATGGTGCAGTATATGTTGAGTAGCTTAAAAGCTGCTGCTGA
    AGCTCACGGTAAGCCTGAATGGGGAAGCACAGGCCCTACTGATGCTGGCCACTATAACAACTGGC
    CAGAAGACACTCAATTTTTCCGCAAAGAAGGTGGTGGATGGGATGGTCCATATGGTGAGTTTTTC
    CTCACTTGGTACTCTCAGATGCTGTTGGAACATGGTGACAGGATTCTCTCATCAGCCACGTCGAT
    CTTTGACAACACTGGAGTTAAGATCTCAGTGAAGGTTGCCGGCATTCACTGGCACTATGGTACAA
    GGTCTCACGCCCCAGAACTCACTGCAGGGTATTACAACACCCGATTCCGTGATGGCTACCTCCCC
    ATTGCTCAAATGCTGGCGCGCCACGGTGCCATCTTTAACTTCACCTGTATCGAGATGCGCGATCA
    CGAGCAGCCACAAGAGGCCCTTTGTGCACCTGAGAAGCTGGTGAAGCAAGTGGCTCTGGCAACGC
    AGAAGGCACAGGTTCCACTTGCCGGCGAAAACGCGCTGCCACGGTACGACGAGTATGCACATGAG
    CAGATCATAAGGGCATCACAATTGGATGTTGATGGTGAGTCTGGTGATAGAGAGATGTGTGCCTT
    CACATACCTGAGGATGAATCCGCATTTGTTTGAACCAAATAACTGGAGGAAGTTTGTGGGGTTTG
    TGAAGAAGATGAAAGAAGGGAAGAGTGCACACAAGTGTTGGGAAGAGGTGGAGAGGGAAGCTGAG
  CATTTTGTGCATGTTACACAGCCTCTTGTGCAAGAGGCTGCAGTGCTGATGCACTGAGAATTGTT
    GAACATCCTTGTGGTAATAGGGCTTAGGAATAAGTCACAAGGAGGCTGTGTGAAAGTTTTAGTGA
    ACCAACAGCCCAGGTTTGTGGCTTTGAAGATGTAAAATTTTGTATTATATTGTTTTGTATTGTAT
    GCACCTAAAACTTCTATTTGTGACCCTTTTACATTGTGTACGTAATCATAGACTTTGGGGTACTG
    TTTCCTTAAAAGTTACTCTACTTTGTACAAGTAGTTACTTAATCTGGTTTAAAAAAATGTCATCC
    CTTAATCTG
 
  Glyma09g29840ATGGCGTTGAGTATGACTCAACAGATCGGGACCCTAGCTGGTGCGACGGTGCCGGATTCCTCGGC
  cDNA SEQ ID   CGGAGAATCGACCGCGGCGGTGAGTGCTGCCGCGGTGTGGAAGTCACCGACGGCGAGTCTGAAGT
  NO: 24); Soybean   GCAAGGTAATGAGGACGGATGGCTGCGCGGAGGGGCTTTCGCCGCCGCTGAGTCCGTGCAGGTCG
  BAM1-1ike   CCGGTGCTGCGGGCGGATCTGTCGGCGGCGTGTCAGGCATTCACGGCGGAGGTGGCGGAGGAGGA
  gene; ATG   GTACGTTGCCGGAGGGAAGGAGGAGAAGGGGAAGGGGAAGGAGGGAGTGCCGGTGTTTGTGATGA
  start codon   TGCCTTTGGACAGCGTGACGGCGGGGAACGCGGTGAACCGGAAAAAGGCGATGAACGCGGCGATG
  and TGA stop   GCTGCGCTGAAGAGCGCGGGGGTGGAGGGGGTGATGATGGACGTGTGGTGGGGTTTGGTGGAGAG
  codon   AGAGAAGCCTGGGGAGTATAATTGGGGAGGGTACGTGGAACTCATGGAAATGGCGAAGAAGCATG
  underlined   GCCTCAAGGTGCAGGCTGTTATGTCATTTCACCAATGTGGCGGTAACGTCGGAGACTCTTGCACT
    ATTCCTTTGCCCAAATGGGTTGTGGAGGAGATTGATAATGACCACGATCTTGCATATACTGATCA
    ATGGGGAAGAAGAAACTATGAATATATATCACTTGGATGTGATACTTTGCCGGTGCTCAAGGGAC
    GATCCCCAGTTCAATGTTATGCTGATTTCATGCGTGCTTTCAGAGACACTTTCAAGCACCTCCTT
    GGTGATACCATTGTGGAAATCCAAGTTGGGATGGGACCAGCAGGTGAGTTGCGTTACCCTTCGTA
    CCCAGAGCAAAATGGGACATGGAAATTCCCAGGAATTGGTGCTTTCCAATGCTATGACAAGTATA
    TGTTGAGTAGCTTAAAAGCTGCTGCTGAAGCTCACGGTAAGCCTGAATGGGGAAGCACAGGCCCT
    ACTGATGCTGGCCACTATAACAACTGGCCAGAAGACACTCAATTTTTCCGCAAAGAAGGTGGTGG
    ATGGGATGGTCCATATGGTGAGTTTTTCCTCACTTGGTACTCTCAGATGCTGTTGGAACATGGTG
    ACAGGATTCTCTCATCAGCCACGTCGATCTTTGACAACACTGGAGTTAAGATCTCAGTGAAGGTT
    GCCGGCATTCACTGGCACTATGGTACAAGGTCTCACGCCCCAGAACTCACTGCAGGGTATTACAA
    CACCCGATTCCGTGATGGCTACCTCCCCATTGCTCAAATGCTGGCGCGCCACGGTGCCATCTTTA
    ACTTCACCTGTATCGAGATGCGCGATCACGAGCAGCCACAAGAGGCCCTTTGTGCACCTGAGAAG
    CTGGTGAAGCAAGTGGCTCTGGCAACGCAGAAGGCACAGGTTCCACTTGCCGGCGAAAACGCGCT
    GCCACGGTACGACGAGTATGCACATGAGCAGATCATAAGGGCATCACAATTGGATGTTGATGGTG
    AGTCTGGTGATAGAGAGATGTGTGCCTTCACATACCTGAGGATGAATCCGCATTTGTTTGAACCA
    AATAACTGGAGGAAGTTTGTGGGGTTTGTGAAGAAGATGAAAGAAGGGAAGAGTGCACACAAGTG
    TTGGGAAGAGGTGGAGAGGGAAGCTGAGCATTTTGTGCATGTTACACAGCCTCTTGTGCAAGAGG
  CTGCAGTGCTGATGCACTGA
 
  Glyma09g29840p   MALSMTQQIGTLAGATVPDSSAGESTAAVSAAAVWKSPTASLKCKVMRTDGCAEGLSPPLSPCRS
  protein SEQ   PVLRADLSAACQAFTAEVAEEEYVAGGKEEKGKGKEGVPVFVMMPLDSVTAGNAVNRKKAMNAAM
  ID   AALKSAGVEGVMMDVWWGLVEREKPGEYNWGGYVELMEMAKKHGLKVQAVMSFHQCGGNVGDSCT
  NO: 25); Soybean   IPLPKWVVEEIDNDHDLAYTDQWGRRNYEYISLGCDTLPVLKGRSPVQCYADFMRAFRDTFKHLL
  BAM1-like   GDTIVEIQVGMGPAGELRYPSYPEQNGTWKFPGIGAFQCYDKYMLSSLKAAAEAHGKPEWGSTGP
  gene;   TDAGHYNNWPEDTQFFRKEGGGWDGPYGEFFLTWYSQMLLEHGDRILSSATSIFDNTGVKISVKV
    AGIHWHYGTRSHAPELTAGYYNTRFRDGYLPIAQMLARHGAIFNFTCIEMRDHEQPQEALCAPEK
    LVKQVALATQKAQVPLAGENALPRYDEYAHEQIIRASQLDVDGESGDREMCAFTYLRMNPHLFEP
    NNWRKFVGFVKKMKEGKSAHKCWEEVEREAEHFVHVTQPLVQEAAVLMH
 
  Glyma16g34360   TATTAGCTAAACTTTGTCATAGGTTGTACGATTATAAAATATCTTTGATAGTTTCACTTATTTCC
  gDNA + about   ATGTACAAATGTTCCTTCTAAAAGGCATGTATTAAGCGTCAAGAACTTAATTAAAAAATTGAGAA
  2.7 kb promoter   TTGGATAACTCGCCAGAAGCAGCCATGAATTTTAACATGAATCAGATGAGCAAGTTCCATTTCTT
  and 5′ UT   ACTTCCCCTACATAATTGGTCCAACAAAATACATAAGAACAATAAACATAGAACTATTGTTGAGG
  sequence(SEQ   AATCAGGAAGACAAACAATGACCATCTAATATCCTTTTAGAGTAGTAGTTGAAGATGCCAATGGC
  ID   AGTTGACAACTAGAAGAACATGTTGAAAAGCAAACGAATAGTTCTTAATTGAGAACAAGCATCAA
  NO: 26) Soybean   AGCACCCTCACATGATTTTTAGAGAAAATGTACCCGATATTTATTCTTCTTTTAAAGGAAGGGTT
  BAM1-like   AAAATTTAAATTATATGAGCAAAATATAACTGTTGTTTTTTTAATAAGAGTAGGCAGAAATATTA
  gene; ATG   AACAATAAAAGGGAGCATAAAGAAAAAAAAAATTGAGATTGCAAAGGTTTATTTTAAAAGCAGAG
  start codon   AAAAGATAGTAACTGCTAACAAAAAGATAACATCACTCACTAACAAATCATGCCTAGAGAATAGG
  and TGA stop   ATCAAAACTGTTTTATCCTATCAGTCAAATGACTTTTATTTTTCCTAAAAAAATAGCATAAAAGT
  codon   CTTATCTACTGTAGTTTCAACAGTCAAATCTTAACAATAACCTTAAATTTAAGGTGGATGATGAC
  underlined   ATTCATCCTTTGAGCTCGCAGTATAATATACCTCAACACAAGTTATTATAGACTCATTCTATGCC
    TTCGGAGTTCGCACTCCTAATAATTATACGCTAACGGATTCATTTATCCATCATATTTTTAAATT
    TCAATTTTCTAATGAAAAAATACTATAACTACTCACTTTTTATTTACACTGTGATTTAATAATAA
    ATTAAAAAAATATTTTTTAGATCATCATCCAATTATAATTTTTTAATGTATAATAAATTTGTTGA
    CTTTCATGATACTTATTTTAAAAAAATTATTAATATTGAATTCTGATTAGATGATATTAAACTCA
    AATAAATTATCATTTATGTTTAATTTATTGATTTTTATAATAATTATATTTAAAATTACATAAAA
    TATAAATTTTTATTGATTAAAAAGTGTAAAAGCTTTTTACAGAAATGGTGGATATCTATTAAACT
    CTTTTATAATAGAATCAAACTAATATTTTAGTACGTGAATTGAATAGAGTAAATGTTTATCTTAT
    AAAACTATCCTTTATAATAATAATAATAAGGCATGCCCGATATTATTATTACTATTATTGAAGGA
    ATATATAAGCATACGCATTTAAAAAAAATACCAAATATACTAGTTTAATTTGTAATCACAATTTT
    TAATCTCTAATCATCTTCAATCTAGGAATAAGTCTCTAGCTATCATATTTAAACTGAGTTTAAAA
    TATTTCACATATTTTGTTAATGTCAAATGACAATGTTTATTTGTTATGAAGTAATCAAAACCACG
    AAACAACAAAACCAAATCTAGCTCTATATTAATCACAAAATAAGTATTATATTAAAAATATCTCA
    AAATAAATATTATATTAATTTTTCAATGTAATATTAATTTTTCTATATTAACATCTTTGATAAGT
    ATCACTTTAAATTTCAATGTAATACTAAAAGTTAGATTTATAAAATTATTATTCTCTTTTATTTG
    TTTATTAACTTTTGTAAATAATTTATGTCAACATTTTTTAAACAAAAAAGAGTAGCTATTATATT
    ATACTATTTTTAAAACATCTACTTTAAAAAAGTATATCATCTATTTATTACTGGTTTAATCATGA
    TTGAATCACAATTGAATCATTAAAATTTAAATAAGTATCATCACTTTTTTTGTCCTACCTATTAT
    AGTCTGCAACTCATATTAAGTTGAATAGCTAATTTTGGGATGTGAAAAAATAGATTTCATGACCA
    TTGGCCGATGACATGACACTTGCCGTGTTCCCAATCTCACAAGATCCTTCTCCTCCCATATTTTC
    TCTTGGCTCCTACATCGACACGTGACCACACATCTCTCACGTGTCACCTTTCCATGGACCATCAC
    CTTCACGCGCAATCCCCATCCCACCGTCCATTCTCCCAAATGACACGTGGCGCAAAACTAACGGT
    CATACCCAAAATATTAATATTACTTATTGTAACCTTATCCTCACCACCCCCTTCCCCCTATAAAT
    ATCCTTCCCCCTCACTGCATTCGCTAAACCCAATAAATTGTTATTTTCTGTTCTTTGCATTTGAA
    TCAAAGCAAATTTTGATTGATTGATTAGAAAATGGCGTTGAATATGACTCACCAGATCGGGACCC
    TGGCTGCTGCGACGGTGCCGGTGCCGAATTCGTCTGCCGGAGAATCAACCGCGGCGATGAGTGCC
    GCCACTCTGTGGAAGCCGCCGGCGGTGAGTCTGAAGTGCAAGGTCACGAGGACGGAGGGCGGCGC
    TGAGGGGCTGTCGCCGCCGCTGAGCCCGTGCAGGTCGCCGGTGCTACGGGCGGATCTGTCGGCAG
    CGTGTCAGGCGTTCACGGCGGAGGTGGCGGCGGAGGAGTACATTGCCGGAGGGAAGGAGAAAGGA
  GAGGGGAAGGAGGGAGTGCCGCTGTTTGTGATGATGCCGTTGGACAGCGTAAAGACGGGAAACGC
    GGTGAACCGGAAGAAGGCGATGAACGCGGCGATGGCGGCGCTGAAGAGTGCGGGGGTGGAGGGGG
    TAATGATGGACGTGTGGTGGGGTTTGGTGGAGAGAGAGAAGCCTGGGGAGTATAATTGGGGAGGG
    TACGTTGAACTCATGGAGATGGCGAAGAAGCATGGCCTGAAGGTGCAGGCCGTTATGTCATTTCA
    CCAATGTGGCGGTAACGTCGGAGACTCTTGCACGTGAGTATTATTATGCAATCTCTCTCATTCTT
    TTTTGTCATTGCTGATTGAATGTTATTAGATTCTGGATCAATTGGTTTTAACAATTAGAATTGTT
    ACTATTAGATTCTGGAGTACTTTAAAGGTTTCTTTTAGGTTTAATTTCTGTGAATTCGTATTGAA
    ATCTGAAAATCAATTGAGTGACACCATGAAATTTTTTTACGTTTTGGAAACATTCTTATTTAAAA
    AAATTTTAACGTCGTGTTTTTGCTTTTAATTATATTTGTAGTTTTTTAAAATAAGCAATTATATT
    TTATTAGTATTAAAATTGCTGGACACGTGAAACAAAACGGCTGGATACATTCTTATTAAAAAAAT
    TTAACGTCAAGTTTAGATACCTAAATATTGTTATACGATATATATCTATAATGTTTGGATAATGA
    AATTGGTCGGACAAGCAATTTGGATGAAAATTCATGCAGTGTGAAAATGTTAATTTTTTGTGAAA
    GTAATTCGTTTAATTTATATTTTAATTTTTATAGTTTAAAATTAATATTTTTAGTTCTTATAATT
    TACATTTTAAATATTAACATATATTTTAATTAATTTCATATATTTATCTTTATAGGAAAATATGT
    GGTTATTAATTATATGGAGTTTTCGATGATTATGATTGAAAATGGGAGGCGTGCCCAGCACGATG
    CAGCCTGTTTGTGAAAAATAAAATAAACGGATAAAAGGGGTTTTGTGATGGGAAATGAAGCCAAT
    ACTGCCATGTGAATGATGTGATATTGGACTTGTATCACTTTGCTTCTAAGTGTAGTATTAGTTTT
    CTCTATTGAATGAACTAGGAACCTGGAATAATTGAATCTTGAGAATTGTGTATATTCATAATTAT
    TTAGCCATTTCCCTTTTACTGAAATTTTAGTGTTTCATTTTTATTACTACTATTTTGATCGAAGA
    TTATGAAGTTAATTAAATTTTAATCCTTGTGCTATTACGAATGAGCTGGCATTCTCTTAAAATTA
    GGGATAACAACAATATTAATTTAGTATTTTTAAGCATGATTATTATGCTTATTAAAAAAACATAA
    TTATTATATCTATTTTAACATAATAACAATGATTAAAAATAATTTCATAAATGTTTATATTTTGA
    TATGATTTAAAGAGGCGTAATGAGCACGGTGCAGAGTCTTATTTTCTTTCATCTTTCGTGGTCCT
    TGTGTGTAGTAAATACAAAATACGTGAGAAAAGAGTGTGCTTTCGTGATGGAAAGTGCCAAAGTG
    GGACCACGTGAGGTAGCACTTGTAGTTCTACTGATTCACGTCGGTATCGCCACAAACAGTAGACT
    AACTTTTTAAGGATCTACTACCTTTAATCAAGTGGACCCGAGATCTTAATTTGTTTTTCAGTCTA
    TTTTTTGAAAATGTATTTGTAAAATATTTTCATTTGTTTAAAATGTTATTTGAAACAAATAATCC
    AGATATATTTTCTGTTTATATATTTCATGTAAATTATTTCAACGGCTATCAATTATAGTAAACTA
    GTTTTCATTTATCAGTGATCGCATAAATCAACTATTGATTTCGAATTTGAGTCTTGGACATGCGG
    TAGTTAAATAGTTGGAGGAGGGTTTAAAATTCACAGTGATTCTATCTGGTTCCAGTAAGAGATAA
    TCCAGTAGAATTATCTCTTACAGGAGATAGCTGTGGTTTATTAAAAAAAAAAAAACTAGTTCATA
    TTTTTATGATTTTAGATATTATTGCCATCAGTTTTGCTGTAAGTTAGCATAGTGTTGGAATACCA
    GTTGTCTTATTGGTTGGCTTATCAGATTGTTTGTCTTGTGTGCAGTATTCCTTTACCCAAATGGG
    TTGTGGAGGAGATTGATAATGACCCCGATCTTGCATATACTGATCAATGGGGAAGAAGAAACTAT
    GAATATATATCACTTGGATGTGATACTTCGCCAGTGCTCAAGGGCCGAACCCCAGTTCAATGTTA
    TGCTGATTTCATGCGTGCTTTCAGAGACACTTTCAAGCACCTCCTTGGTGACACCATTGTGGTAA
    ATATCTTTCTCAGTGCACTTTTACATCATGGTGTGATTTTTGTTGCTATATAACTTCTCATCTAA
    ACTCCTTTTACTGGCATATTTCAGGAAATTCAAGTTGGGATGGGACCGGCAGGTGAGCTGCGTTA
    CCCTTCTTACCCAGAGCAAAATGGGACATGGAATTTCCCAGGAATTGGTGGTTTCCAATGCTATG
    ACAAGGTATATATATTTACGTTTTTTTTTCCTTCTCCTTCTTGTACTCTTTTATATATAATTGTT
    TTAGGATTTGTTTGGATAAATTTCTTGATGAACGAAGAGGAGAAAATTAGGTAAAATGTGTTCTA
    ATACTTAAATTATGCTACGGTGCAGTATATGTTGAGTAGCTTAAAAGCTGCTGCTGAAGCTGAGG
    GTAAGCCTGAATGGGGAAGCACAGGCCCTACTGATGCTGGACACTATAACAACTGGCCAGAAGAC
    ACTCAATTTTTCCGCAAAGAAGGTGGAGGCTGGGATGGTCCATATGGTGAGTTTTTCCTCACCTG
    GTACTCTCAGATGCTGTTGGACCACGGTGACAGGATTCTCTCATCAGCCACGTCAATCTTTGACA
    ACACTGGAGTGAAGATCTCAGTGAAGGTTGCTGGCATTCACTGGCACTATGGCTCAAGGTCTCAC
    GCCCCAGAACTCACAGCAGGGTATTACAACACCCGGTTCCGTGATGGCTACATCCCCATTGCTCA
    AATGTTGGCACGCCACGGTGCCATCTTCAACTTCACCTGTATTGAGATGCGCGATCACGAGCAGC
    CACAAGATGCCCTTTGTGCACCCGAGAAGCTTGTGAAGCAAGTGGCTCTGGCAACGCAGAAGGCA
    CAGGTTCCACTTGCTGGTGAAAATGCGCTGCCACGGTACGATGAGTATGCTCATGAGCAGATCAT
    AAGGGCATCACAGTTGGATGTTGATGGTGACTCTGGTGGAAGAGAGATGTGTGCATTCACTTACC
    TGAGAATGAACCCGCATTTGTTTGAACCAAATAACTGGAGGAAGTTTGTGGGGTTTGTGAAGAAA
    ATGAAAGAAGGGAAGAGTGCACACAAGTGTTGGGAAGAGGTGGAGAGGGAAGCTGAGCATTTTGT
  GCATGTTACACAGCCTCTTGTGCAAGAAGCTGCAGTGCTGATGCACTGAGAATTGTTGAACAATC
    TTGTGCTGATAGATGGCTTAGAAAAGGTCACAAGTAGGCTGTGTGAAAGTTTTAGTGAACCAGCA
    GCCCAGGTTTGTGGCTTTGAAGATGTAAAATTTTGTATTATATTGTTGTTTTATATTCTATGCAC
    CTAAAACTTCTATTTGTTACCCTTTTATATTGTGTACGTAATCATTGACTTTGGGGTACTATTTT
    CTTAAAAGTTACTCTACTTTGTACAAGTAGTTACTTATTTCTGCATCATGAAACTGTTACATGGC
    GTAACAGCAACAAGAGATGCTATTTTCTTCTATAGGGAAAAATGAATTTAAAATCAATGATTTTC
    GTTGTGTTT
 
  Glyma16g34360ATGATGCCGTTGGACAGCGTAAAGACGGGAAACGCGGTGAACCGGAAGAAGGCGATGAACGCGGC
  cDNA (SEQ ID   GATGGCGGCGCTGAAGAGTGCGGGGGTGGAGGGGGTAATGATGGACGTGTGGTGGGGTTTGGTGG
  NO: 27) Soybean   AGAGAGAGAAGCCTGGGGAGTATAATTGGGGAGGGTACGTTGAACTCATGGAGATGGCGAAGAAG
  BAM1-like   CATGGCCTGAAGGTGCAGGCCGTTATGTCATTTCACCAATGTGGCGGTAACGTCGGAGACTCTTG
  gene; ATG   CACTATTCCTTTACCCAAATGGGTTGTGGAGGAGATTGATAATGACCCCGATCTTGCATATACTG
  start codon   ATCAATGGGGAAGAAGAAACTATGAATATATATCACTTGGATGTGATACTTCGCCAGTGCTCAAG
  and TGA stop   GGCCGAACCCCAGTTCAATGTTATGCTGATTTCATGCGTGCTTTCAGAGACACTTTCAAGCACCT
  codon   CCTTGGTGACACCATTGTGGAAATTCAAGTTGGGATGGGACCGGCAGGTGAGCTGCGTTACCCTT
  underlined   CTTACCCAGAGCAAAATGGGACATGGAATTTCCCAGGAATTGGTGGTTTCCAATGCTATGACAAG
    TATATGTTGAGTAGCTTAAAAGCTGCTGCTGAAGCTGAGGGTAAGCCTGAATGGGGAAGCACAGG
    CCCTACTGATGCTGGACACTATAACAACTGGCCAGAAGACACTCAATTTTTCCGCAAAGAAGGTG
    GAGGCTGGGATGGTCCATATGGTGAGTTTTTCCTCACCTGGTACTCTCAGATGCTGTTGGACCAC
    GGTGACAGGATTCTCTCATCAGCCACGTCAATCTTTGACAACACTGGAGTGAAGATCTCAGTGAA
    GGTTGCTGGCATTCACTGGCACTATGGCTCAAGGTCTCACGCCCCAGAACTCACAGCAGGGTATT
    ACAACACCCGGTTCCGTGATGGCTACATCCCCATTGCTCAAATGTTGGCACGCCACGGTGCCATC
    TTCAACTTCACCTGTATTGAGATGCGCGATCACGAGCAGCCACAAGATGCCCTTTGTGCACCCGA
    GAAGCTTGTGAAGCAAGTGGCTCTGGCAACGCAGAAGGCACAGGTTCCACTTGCTGGTGAAAATG
    CGCTGCCACGGTACGATGAGTATGCTCATGAGCAGATCATAAGGGCATCACAGTTGGATGTTGAT
    GGTGACTCTGGTGGAAGAGAGATGTGTGCATTCACTTACCTGAGAATGAACCCGCATTTGTTTGA
    ACCAAATAACTGGAGGAAGTTTGTGGGGTTTGTGAAGAAAATGAAAGAAGGGAAGAGTGCACACA
    AGTGTTGGGAAGAGGTGGAGAGGGAAGCTGAGCATTTTGTGCATGTTACACAGCCTCTTGTGCAA
  GAAGCTGCAGTGCTGATGCACTGA
 
  Glyma16g34360   MMPLDSVKTGNAVNRKKAMNAAMAALKSAGVEGVMMDVWWGLVEREKPGEYNWGGYVELMEMAKK
  protein(SEQ ID   HGLKVQAVMSFHQCGGNVGDSCTIPLPKWVVEEIDNDPDLAYTDQWGRRNYEYISLGCDTSPVLK
  NO: 28) Soybean   GRTPVQCYADFMRAFRDTFKHLLGDTIVEIQVGMGPAGELRYPSYPEQNGTWNFPGIGGFQCYDK
  BAM1-like gene   YMLSSLKAAAEAEGKPEWGSTGPTDAGHYNNWPEDTQFFRKEGGGWDGPYGEFFLTWYSQMLLDH
    GDRILSSATSIFDNTGVKISVKVAGIHWHYGSRSHAPELTAGYYNTRFRDGYIPIAQMLARHGAI
    FNFTCIEMRDHEQPQDALCAPEKLVKQVALATQKAQVPLAGENALPRYDEYAHEQIIRASQLDVD
    GDSGGREMCAFTYLRMNPHLFEPNNWRKFVGFVKKMKEGKSAHKCWEEVEREAEHFVHVTQPLVQ
    EAAVLMH
 
  Glyma01g40590   TTGAGAACTTAACCTACTAAAATTATTCTTTGATGTAATGTTAATGATTTTTTTATTTATAATTA
  gDNA + about   TTCTAATTTAAATATGCATCTACTAGTATATTCTAATTTTACTCCCCAACATAAAAAAGTCTAAT
  5 kb upstream   TTATCTATTTTCTCTCTCAAATCCCTTTACAAAACTAAAATAGTAAATTGCATTAAAAATATAGA
  promoter and   TGTATAACATGCTAAAGAAAAATTAATGTTTCCCCATGTTACCCCTAAAACTTATCATGCAAATG
  5′UT sequence   GATGATCAAGTCATAAGAAATGTAATATTCATAAATAGATAAGAAGATAAATTACATCAAAAGTA
  (start and   GTTGACGGTCAAATTTTCAACAAAAAAGGTTTAGCCTCTTATTGTCATGGAGATTTTATAATTGC
  stop codons   AAGAGTAAAATATTTAGTAAAGGGGAGAAAATAAAAAAGGGAATAAAGGAAATGAATGACTCTCA
  underlined;   ATATTTATTTCTCCTTCTTCTAGTCTTTGCCTTCTATAATGAAGTGTATTCTCTCTTAAAAATTT
  SEQ ID NO: 29);   TCCTTTGTTTTTTCTTATTCTCTCCTTTTCTTTTATAGATGCATATTAGTGGGCTTCTTGCATTA
  Soybean BAM2-   AGTCTAAGTCTGTCTTTATTTTTCTTAATTAGTCATATTTTTCTTAATTAGTTCGCTTTCCTTAA
  like gene   TTATTCCTCTCTTCTTGAATTATCCTACTTTTTTTTTACTCACTAAGCATAATAAATTCATCATT
    TTTAATATTTGTTGCACAAAAAATAAAATAATGTTAATTTAACAATTATTTGCTTAAAAAAAATT
    AGAAGAAAAAAATTACAAATTCTTATATATTTTAACCCTCAAAATATACTTATAATTAGTTGTTA
    TTGATTTTAAAGTTAACCTATTTTTTCAAGATATCCATGGTAGGTATTTTCAAATTACACACTTC
    ACATGTAAACTTTGAGGTTGCAAGGGTGAAAACAGGTAAAAAGAATAACAGCTAGCAAAGACATT
    TAAAATAATTCTAGCAATATAAGTCCAATCTAAAGCGGATACGTCCAGCAATATTCATCCCTCAC
    CAACTCCAACTTCACTCTCAATAAACTGGAAAATTATAACCAAACATGCTGAATCGTGAAGGCAT
    CCCTACAATTCCTTCCTAGCCAACCAGCCCAACAATTTTCTTAGCTTTTAGAAATATTATCGTGT
    GCAATGTGATACACTGCAGTAAGCATCAACAAGAATAGTAACCTGACCTTTCATGCCATATATGA
    TCGAAGTGGTCAAGAATGGCAAGTAGAAGTGAGGTTCATGCTCTTTAATGATTAATCTAATGGGA
    TAAAAAGGACAAAGACAAACAGAACTCTTAATAGAAAAGAAAAAAACTAAGTGGGTCAACAATGC
    ATATTTTGGATTCAAAACCACCACTGTCCAATCGACAACATTGTTCTACAAAACCGGAATGATTG
    TGATTCATCCGGAGGGTATTTGCTCATTCATGTTCCTTATTGTCGATATGGGCATGCCTAACTAG
    CTAAGTACAATTTCCTTAATTTCTATTTTGGCACTTACAATCGTAATTAAAACTGAAATCAGGTT
    TATATATATATATATATATATATATATATATATATATATATATATATATATATATAAATTAGCAT
    GCATTATATATTTAAGGGGTACGGGTAACGTGTGTACAATATACTCCTTACAAAAGGTTTATATC
    TCTGCTCGGCTTCTTATCCCAAAATTAGCAAGCATTAAATGAAGGGTAACGTGTGTTTTGTTCTT
    ATTAAAAAAAAAACATAGTACAATTTTTTAAGTGGAAACATGGAAATATTTTTCACTCTTTTTAA
    TGATTTTTTTATAACATAAAATTAAAATATTAATTCTAAAGTAGCCAATAATTATAAATTTTTCA
    CTAACTATGTATTGTAATGAAAAAAAATATTTTTATATTTTACTTTTGGAAAATTTTAAATTTAT
    TTGTTAGCAAATGATCTGTTCATGATATATTTTTATTAATTTTAAATATTATAATTTAAAACATA
    TATATTTAAATTTAATTTTCTGATACAACATTGGAGGATTATATATATAACTGCTCAGATAGACC
    CCTCCAATAGTCCAATTGTAATAAGAGTTTGAGAACATAAGAAAAAAAATCTTGTAATTACTAAT
    CTATATTGTGGCTTTCCTCATACAATTGATCCATGGAGAGAAGGAGTAATTCACAATAATAATAG
    TAATTATTAGTATTATAATAAATGTTAATGTTGGTGACTGCTTGGTCATTTTCTCTTCCAGAAAA
    ACAGATAAGCTGTGACCTGTTAGTAAGGCCATGGTGGGAGGGACCACTGCATGGCATCTTTCTCA
    GTGCTACTAGTGCTTCACTTATTACATGATTTTGAAGTTGTCAGTGAGCGGGTAGAAGATGGAGG
    CCATGGTCCACACTTTGTTGCCGCATTGCAAGAAAATGGTAAAAATGATATTGAATCTGCAACCC
    CCCAATGTAAGGGCCTCTTGTAATAATGGAAGCAGCACAGGGGCGAAGTCACACATTGATAATAG
    GGTTTATCGAAAACACCACATCACACCATACCACTTCACTTACCACGCCCCCTCTCTTTTCGTGT
    CAACAATCTTTGACCACCTTTATCCAACCTAACAAAATCATTACTGTTTATTAATTTTATACTCT
    TGTTTTACTAGTAATTTTCTATATTGATTTCGTTCATTTGTTATGCAGGTGTGAAAATGAACACG
    ATCAATAAAAGAAAGGAAGAAAAATCTAGCCTTTAGTGATGATATCGGACTTCTTTTTTGTTTTT
    TCAAAAGGAGGGCTTGCAATTCGACAATAACTAAGCAAAATTAACAAAAATTAAAGAAACAATAA
    TCCATTTTCTGTCATAATTTCGTGCTTTGATAAATTTAATACTGCAATATTATTGTAGAACCCGT
    GATTATGAAGTATAAGAACATAAACTTCATGTGATAAATTTTCACTGCAAATAGAATGTCTATAT
    GTTTTTCATTTAAGACACACTATTACAAAAAACAATCTTTGAACGACGATTCATTGACACATTTA
    ATAATTGTTTTTAACCGTTATTGAAGTGAATGTAATGAGAAATATTATATTTTTTACGATAATTT
    CTTAATCATCTTAGAAGATCTCATCTTTTAAGATAACTTTTATGTTAAAACCGTTGTAGAAGACC
    CACCATCCTAAAAGAACACTAACTAGAAAAAGAATGGTGGAGAGGGTGAAATAGCTACGGGTTCC
    TTGGCTTAGTGTACAGTTTGGCGGGACCTTACTCCTTGGGAAGGCTAGACAGTAGAAGGATACTC
    CGAGATCACTTCAAAGAGAATACGACACCCATGATTATCAAAAGGTTAGACAAGTTGATGCGCAA
    TTTCCTTGTTTTCAGTCATAATTTTGGACTAATTAAACTCCACACAGAACGACAAGCATGCTTAT
    TTTCTAGGCTTTTGCTTTGCTGAATACTAGAAGATAAATCTCATAGCTTTAGCCCATTGCCAAAC
    GCTGGATTTTACTCTCTTCCTCACAAGATGGTAACAAGTTAGATAATCTAAGATTTGTGACCTTA
    TTCGTCTTATGTTTGGGTTAATATTCATGTTGTACCGAGTATCATGTGCTCTAAAACATGCAGTT
    TTGGCTTGGCAATGAATTAGAAGTATTCCATCAAAGTAATTCATACCATACCCCATTTTTAAAGC
    TCAAAATGAGCAAGATAAAAACTTTAAACGTATCTTAGGGCATTCATTATTATCAAAAGCCTTTA
    TATTCATTAGAACTCTTTGCATGTATAGACCATTTTCTCTTTTTTAAATAAAAAACATATTAACA
    TATGTATCTCAGGGAATTTATTAAACAATTAAAAATGAAAATATTTATATAAAAATATTATCGAC
    ATAATATTATTATTATTATTATTATTATTATTATTATTATTATTATATATCGTGAGTTTTAATTA
    AAAAAAATTCATTGATACTCTTTAAAGTAGAAACGCTTGTTAGTAAAAATGATATTTTTGAATTT
    AAAAGGTTATACATTTTTATATTATTGTTAAAATTTAAAACTTAATAATGAAAATTAAAAATATT
    TATTTTTATCCTCAAATGACTAGACACTACAACAAAATAAATAATAAATAAGACAAGGAAAACTA
    ACAAAAGAACTAACCGTTGTCCTTGACCTTCCTTGGAAAATAAGGCAATAGCATAGGACCTACTT
    CAAAAAAGACATTCGACTACAAAAACATGCAAAATGGACAAAGATGAACAGAAAAACTAAGAAGA
    CGTTGCATTTATTTTTTCAATTTCACGTATTTTCATTGAAAATTATATTTTAACATTATTCATTT
    ATTTGTTAACAGGCCTATTTTAAAATTCGAAACCTCGGTATTTTATTAAACTCATTAAAATATCT
    ACACCATTTTTTATTAAAAATATAATAATAATAATTTTAATATAGTTTCTTAATAATAAAATCTC
    TAATAACTGCGAAAAAAGTATTTTTCTAAAAATACCATAATTAAATACGTACAACAACGAAGTAT
    TAAACATATAAAACTAAAGAACCACGACACATTTATGTCTTTCCTATCACAATCATAAGTAATGC
    TTGATTTGTGAGCACACTCTCCATAACCAACAACACACACATAACATTCTTTTATTAAAATCATT
    TTAAATTATGTCACATAATAACTACTGTAACAACACACATTAGCATGAAACTGGTATTAGTAGCA
    CATACAATAAATAAATATTGATTATTATCTGATGTAATTATGTAAGTATTATGAGTGGTTGATTA
    AAAAAACAAAATAGAGTTGGTAAGGGGGTGGATCCACATCCACCGCTTCTGCACCAAACTCAGCA
    TAGCAGTGGGTCAATGATTGATTGGTAATTGTAATTCTATTCAAAAAGTGAAAAGAGTTGAATGA
    GAATTCGTATATTCAGAAAATCCCCCCTCCTTTAAGATAAGAGAATAGGCCTCACTCTTTCTTTC
  TCTTCCATTCCCAAAATGCGTGTCCTCTTTCTTTTTCTGTTTTTCCAGTTTCTCCATTTTCATTT
    CCCCAAAACCCTTTCAGCCCCAATCTCAGAGTACCGTGCCCTTCTCTCTCTCCGTTCAGCCATTA
    CCGACGCCACCCCACCTCTTCTCACTTCGTGGAACTCCTCCACCCCTTACTGTTCCTGGCTCGGC
    GTCACCTGCGACAACCGCCGCCACGTCACCTCCCTAGACCTCACCGGCCTCGACCTCTCCGGCCC
    CCTCTCCGCCGACGTCGCCCACCTCCCATTCCTCTCCAACCTCTCCCTCGCCTCGAATAAGTTCT
    CCGGCCCCATTCCTCCCTCACTCTCCGCTCTCTCCGGCCTCCGCTTCCTCAACCTCTCCAACAAT
    GTCTTCAACGAAACCTTCCCCTCGGAGCTCTCGCGCCTCCAGAACCTCGAGGTCCTCGACCTCTA
    CAACAACAACATGACCGGCGTGCTTCCCCTCGCCGTCGCGCAGATGCAGAATCTTCGTCATTTGC
    ATCTCGGCGGCAACTTCTTCTCCGGCCAGATCCCGCCGGAGTATGGACGCTGGCAGCGCCTCCAG
    TACCTCGCCGTCTCCGGCAACGAGCTCGAGGGGACTATCCCTCCGGAGATCGGAAACTTGTCCAG
    CCTCCGGGAGCTCTACATCGGCTACTACAACACCTACACCGGGGGCATTCCGCCGGAGATCGGAA
    ATTTGTCGGAGCTGGTGAGGCTCGACGCCGCCTACTGTGGGTTGTCCGGCGAGATTCCGGCGGCG
    CTGGGAAAGCTTCAGAAGCTGGACACGCTGTTCCTTCAGGTGAATGCATTGTCAGGGTCTTTGAC
    TCCCGAGCTGGGGAACCTGAAGAGCCTGAAATCCATGGATTTGTCTAACAACATGCTCTCCGGTG
    AGATTCCGGCGAGATTCGGCGAGCTGAAGAATATTACTCTTCTGAATCTGTTCAGGAACAAGCTT
    CACGGAGCTATACCAGAGTTTATAGGGGAGCTTCCAGCGTTGGAAGTTGTGCAACTGTGGGAGAA
    TAACTTCACAGGTAGCATTCCAGAGGGTTTGGGCAAAAACGGGAGACTCAACCTTGTTGATCTTT
    CTTCTAACAAGTTAACTGGGACTTTGCCTACTTATCTCTGTTCTGGGAATACTCTTCAGACTCTG
    ATAACTCTTGGGAATTTTCTTTTTGGTCCAATTCCTGAGTCGCTTGGTAGTTGTGAATCCCTTAC
    ACGGATTAGAATGGGAGAGAACTTTTTGAATGGTTCCATTCCGAGAGGGCTTTTTGGACTTCCCA
    AACTAACACAGGTTGAGCTTCAGGATAATTATCTCTCTGGAGAGTTTCCTGAGGTGGGTTCTGTT
    GCTGTTAATCTTGGTCAGATTACTCTCTCTAACAACCAGCTTTCTGGGGTTCTACCTCCCTCCAT
    TGGTAACTTCTCCAGCGTGCAGAAGCTCCTTCTTGATGGCAACATGTTCACGGGTCGGATACCTC
    CCCAGATTGGGAGGTTGCAACAGCTTTCTAAGATTGATTTTAGTGGCAACAAGTTCTCGGGTCCT
    ATTGTGCCTGAGATCAGTCAGTGTAAGCTGTTAACTTTCCTTGACCTTAGCCGCAATGAGCTATC
    TGGAGACATCCCAAATGAGATAACTGGCATGAGGATATTGAATTACTTGAATCTTTCTAGGAATC
    ATTTAGTGGGTGGCATTCCCTCTTCGATATCATCTATGCAAAGCTTGACTTCTGTTGATTTTTCA
    TACAACAACCTGTCTGGTTTGGTGCCTGGTACCGGTCAATTCAGCTACTTCAATTACACGTCTTT
    CTTGGGAAACCCTGACCTCTGTGGCCCCTATTTGGGTGCTTGCAAGGATGGGGTTGCCAATGGCG
    CACACCAACCTCATGTTAAAGGTCTCTCCTCTTCTTTTAAGCTGCTACTTGTTGTTGGGTTGCTA
    CTATGTTCCATTGCTTTTGCTGTGGCTGCAATATTCAAGGCCCGGTCACTGAAGAAGGCCAGTGG
    GGCTCGTGCATGGAAGTTGACTGCGTTCCAACGTTTGGACTTCACTGTCGATGATGTTTTGCATT
    GCTTGAAGGAGGATAATATTATAGGGAAAGGAGGTGCTGGCATTGTCTACAAAGGGGCTATGCCT
    AATGGGGATCATGTTGCTGTGAAAAGGCTTCCGGCTATGAGTAGAGGCTCTTCACATGATCATGG
    CTTCAATGCTGAGATTCAAACATTGGGGCGAATCCGACACAGGCACATTGTTAGGTTGTTGGGCT
    TCTGTTCAAATCATGAGACAAACCTTTTGGTCTATGAGTACATGCCCAATGGAAGTTTAGGCGAG
    GTTCTTCATGGAAAGAAAGGGGGTCATTTGCATTGGGATACAAGGTATAAAATTGCGGTGGAGGC
    TGCCAAGGGGCTTTGCTATCTGCACCATGATTGTTCGCCACTCATTGTCCATCGTGATGTGAAGT
    CAAACAACATCCTTCTTGATTCTAATCATGAAGCCCATGTTGCTGATTTTGGGCTTGCTAAGTTC
    CTGCAAGATTCTGGGACATCTGAATGCATGTCTGCTATTGCTGGTTCATATGGATACATAGCTCC
    AGGTACCGTCCAATTTCGACATAATTAATGCATTATTTACATGGTTGTGGAAAATTTTCTTTTAC
    CCGCCTGTTCATAATTGTACGTTTAATCATTGTTCAGAATTTGACTCTTTGACTTATCATCATGT
    TTTAGGTGTAGACTGTTGATATTGAGGTGATGTCCCTAAATTAATTAACATTGCTATGTGGTTTT
    TCTTGACTTTGGTTTTCTATCATACCCAAATGATCTCTTGATTTCGACCCCTTATTTAGTCTATT
    TCAAGCCAAGTACTGAAAGTAAATGGTAGATAGCTCTGCAACGTTAGAGTCATTCACGACCGGAA
    ACTGATGATTATGGGCAAAATATCGGATAAAAAGACCTATTATGTTACTTTACACTTATTGCCTT
    TGTTTAACTTATAGTTTCAAATTCAAGTGTCTTGCTTTATTTTAGTTTATGATACATGTTCGATG
    TTTGATTGCAGAGTATGCCTACACATTGAAAGTTGATGAGAAAAGCGATGTGTACAGTTTTGGTG
    TGGTTCTCTTAGAACTTATAACAGGCAGGAAACCAGTTGGAGAATTTGGTGATGGCGTGGACATA
    GTGCAATGGGTGAGGAAAATGACGGATTCTAACAAGGAAGGAGTTCTTAAAGTTCTTGATCCTAG
    ACTTCCCTCAGTTCCCCTTCACGAAGTGATGCATGTTTTCTATGTAGCCATGCTGTGCGTTGAAG
    AACAGGCTGTAGAGAGACCAACTATGCGTGAAGTTGTTCAAATACTGACAGAGCTTCCAAAGCCA
    CCTGACTCTAAAGAGGGGAACTTAACAATAACGGAATCATCTTTGTCATCATCAAACGCTTTAGA
    ATCTCCATCCTCAGCCTCTAAGGAAGATCAAAATCCTCCTCAATCCCCACCACCCGATCTTCTTA
  GCATTTAAAGTGCTCTGTTGGGTGTTTCATCTTAGTTCCCTTGGGTTGTGATCGCTTATCCATTT
    ACTTTCTTTTTCTGTCTCTCTTCTGGGATTGGTTTTTTTTTTTTTCCCTAACTGAAGGTGTTAAT
    GTTTGGATTTTTTAATGGTTTTGTACAGTAGGATTGATGGGGGTATTTTCTTATAAAGTCACTGT
    CTTCATCATGTAGTACTGCTTTTTAATTTTTATTTGCGACCGTTGTTGGGGAGGATTCAAGGGAT
    ACAATTAAATTACTCGTTTGTTTCCTGAAATTTCATTATTCATACTTTTTTAGTTTATG
 
  Glyma01g40590ATGCGTGTCCTCTTTCTTTTTCTGTTTTTCCAGTTTCTCCATTTTCATTTCCCCAAAACCCTTTC
  cDNA (start and   AGCCCCAATCTCAGAGTACCGTGCCCTTCTCTCTCTCCGTTCAGCCATTACCGACGCCACCCCAC
  stop codons   CTCTTCTCACTTCGTGGAACTCCTCCACCCCTTACTGTTCCTGGCTCGGCGTCACCTGCGACAAC
  underlined;   CGCCGCCACGTCACCTCCCTAGACCTCACCGGCCTCGACCTCTCCGGCCCCCTCTCCGCCGACGT
  SEQ ID NO: 30);   CGCCCACCTCCCATTCCTCTCCAACCTCTCCCTCGCCTCGAATAAGTTCTCCGGCCCCATTCCTC
  Soybean BAM2-   CCTCACTCTCCGCTCTCTCCGGCCTCCGCTTCCTCAACCTCTCCAACAATGTCTTCAACGAAACC
  like gene   TTCCCCTCGGAGCTCTCGCGCCTCCAGAACCTCGAGGTCCTCGACCTCTACAACAACAACATGAC
    CGGCGTGCTTCCCCTCGCCGTCGCGCAGATGCAGAATCTTCGTCATTTGCATCTCGGCGGCAACT
    TCTTCTCCGGCCAGATCCCGCCGGAGTATGGACGCTGGCAGCGCCTCCAGTACCTCGCCGTCTCC
    GGCAACGAGCTCGAGGGGACTATCCCTCCGGAGATCGGAAACTTGTCCAGCCTCCGGGAGCTCTA
    CATCGGCTACTACAACACCTACACCGGGGGCATTCCGCCGGAGATCGGAAATTTGTCGGAGCTGG
    TGAGGCTCGACGCCGCCTACTGTGGGTTGTCCGGCGAGATTCCGGCGGCGCTGGGAAAGCTTCAG
    AAGCTGGACACGCTGTTCCTTCAGGTGAATGCATTGTCAGGGTCTTTGACTCCCGAGCTGGGGAA
    CCTGAAGAGCCTGAAATCCATGGATTTGTCTAACAACATGCTCTCCGGTGAGATTCCGGCGAGAT
    TCGGCGAGCTGAAGAATATTACTCTTCTGAATCTGTTCAGGAACAAGCTTCACGGAGCTATACCA
    GAGTTTATAGGGGAGCTTCCAGCGTTGGAAGTTGTGCAACTGTGGGAGAATAACTTCACAGGTAG
    CATTCCAGAGGGTTTGGGCAAAAACGGGAGACTCAACCTTGTTGATCTTTCTTCTAACAAGTTAA
    CTGGGACTTTGCCTACTTATCTCTGTTCTGGGAATACTCTTCAGACTCTGATAACTCTTGGGAAT
    TTTCTTTTTGGTCCAATTCCTGAGTCGCTTGGTAGTTGTGAATCCCTTACACGGATTAGAATGGG
    AGAGAACTTTTTGAATGGTTCCATTCCGAGAGGGCTTTTTGGACTTCCCAAACTAACACAGGTTG
    AGCTTCAGGATAATTATCTCTCTGGAGAGTTTCCTGAGGTGGGTTCTGTTGCTGTTAATCTTGGT
    CAGATTACTCTCTCTAACAACCAGCTTTCTGGGGTTCTACCTCCCTCCATTGGTAACTTCTCCAG
    CGTGCAGAAGCTCCTTCTTGATGGCAACATGTTCACGGGTCGGATACCTCCCCAGATTGGGAGGT
    TGCAACAGCTTTCTAAGATTGATTTTAGTGGCAACAAGTTCTCGGGTCCTATTGTGCCTGAGATC
    AGTCAGTGTAAGCTGTTAACTTTCCTTGACCTTAGCCGCAATGAGCTATCTGGAGACATCCCAAA
    TGAGATAACTGGCATGAGGATATTGAATTACTTGAATCTTTCTAGGAATCATTTAGTGGGTGGCA
    TTCCCTCTTCGATATCATCTATGCAAAGCTTGACTTCTGTTGATTTTTCATACAACAACCTGTCT
    GGTTTGGTGCCTGGTACCGGTCAATTCAGCTACTTCAATTACACGTCTTTCTTGGGAAACCCTGA
    CCTCTGTGGCCCCTATTTGGGTGCTTGCAAGGATGGGGTTGCCAATGGCGCACACCAACCTCATG
    TTAAAGGTCTCTCCTCTTCTTTTAAGCTGCTACTTGTTGTTGGGTTGCTACTATGTTCCATTGCT
    TTTGCTGTGGCTGCAATATTCAAGGCCCGGTCACTGAAGAAGGCCAGTGGGGCTCGTGCATGGAA
    GTTGACTGCGTTCCAACGTTTGGACTTCACTGTCGATGATGTTTTGCATTGCTTGAAGGAGGATA
    ATATTATAGGGAAAGGAGGTGCTGGCATTGTCTACAAAGGGGCTATGCCTAATGGGGATCATGTT
    GCTGTGAAAAGGCTTCCGGCTATGAGTAGAGGCTCTTCACATGATCATGGCTTCAATGCTGAGAT
    TCAAACATTGGGGCGAATCCGACACAGGCACATTGTTAGGTTGTTGGGCTTCTGTTCAAATCATG
    AGACAAACCTTTTGGTCTATGAGTACATGCCCAATGGAAGTTTAGGCGAGGTTCTTCATGGAAAG
    AAAGGGGGTCATTTGCATTGGGATACAAGGTATAAAATTGCGGTGGAGGCTGCCAAGGGGCTTTG
    CTATCTGCACCATGATTGTTCGCCACTCATTGTCCATCGTGATGTGAAGTCAAACAACATCCTTC
    TTGATTCTAATCATGAAGCCCATGTTGCTGATTTTGGGCTTGCTAAGTTCCTGCAAGATTCTGGG
    ACATCTGAATGCATGTCTGCTATTGCTGGTTCATATGGATACATAGCTCCAGAGTATGCCTACAC
    ATTGAAAGTTGATGAGAAAAGCGATGTGTACAGTTTTGGTGTGGTTCTCTTAGAACTTATAACAG
    GCAGGAAACCAGTTGGAGAATTTGGTGATGGCGTGGACATAGTGCAATGGGTGAGGAAAATGACG
    GATTCTAACAAGGAAGGAGTTCTTAAAGTTCTTGATCCTAGACTTCCCTCAGTTCCCCTTCACGA
    AGTGATGCATGTTTTCTATGTAGCCATGCTGTGCGTTGAAGAACAGGCTGTAGAGAGACCAACTA
    TGCGTGAAGTTGTTCAAATACTGACAGAGCTTCCAAAGCCACCTGACTCTAAAGAGGGGAACTTA
    ACAATAACGGAATCATCTTTGTCATCATCAAACGCTTTAGAATCTCCATCCTCAGCCTCTAAGGA
  AGATCAAAATCCTCCTCAATCCCCACCACCCGATCTTCTTAGCATTTAA
 
  Glyma01g40590   MRVLFLFLFFQFLHFHFPKTLSAPISEYRALLSLRSAITDATPPLLTSWNSSTPYCSWLGVTCDN
  protein (SEQ ID   RRHVTSLDLTGLDLSGPLSADVAHLPFLSNLSLASNKFSGPIPPSLSALSGLRFLNLSNNVFNET
  NO: 31);   FPSELSRLQNLEVLDLYNNNMTGVLPLAVAQMQNLRHLHLGGNFFSGQIPPEYGRWQRLQYLAVS
  Soybean BAM2-   GNELEGTIPPEIGNLSSLRELYIGYYNTYTGGIPPEIGNLSELVRLDAAYCGLSGEIPAALGKLQ
  like gene   KLDTLFLQVNALSGSLTPELGNLKSLKSMDLSNNMLSGEIPARFGELKNITLLNLFRNKLHGAIP
    EFIGELPALEVVQLWENNFTGSIPEGLGKNGRLNLVDLSSNKLTGTLPTYLCSGNTLQTLITLGN
    FLFGPIPESLGSCESLTRIRMGENFLNGSIPRGLFGLPKLTQVELQDNYLSGEFPEVGSVAVNLG
    QITLSNNQLSGVLPPSIGNFSSVQKLLLDGNMFTGRIPPQIGRLQQLSKIDFSGNKFSGPIVPEI
    SQCKLLTFLDLSRNELSGDIPNEITGMRILNYLNLSRNHLVGGIPSSISSMQSLTSVDFSYNNLS
    GLVPGTGQFSYFNYTSFLGNPDLCGPYLGACKDGVANGAHQPHVKGLSSSFKLLLVVGLLLCSIA
    FAVAAIFKARSLKKASGARAWKLTAFQRLDFTVDDVLHCLKEDNIIGKGGAGIVYKGAMPNGDHV
    AVKRLPAMSRGSSHDHGFNAEIQTLGRIRHRHIVRLLGFCSNHETNLLVYEYMPNGSLGEVLHGK
    KGGHLHWDTRYKIAVEAAKGLCYLHHDCSPLIVHRDVKSNNILLDSNHEAHVADFGLAKFLQDSG
    TSECMSAIAGSYGYIAPEYAYTLKVDEKSDVYSFGVVLLELITGRKPVGEFGDGVDIVQWVRKMT
    DSNKEGVLKVLDPRLPSVPLHEVMHVFYVAMLCVEEQAVERPTMREVVQILTELPKPPDSKEGNL
    TITESSLSSSNALESPSSASKEDQNPPQSPPPDLLSI
 
  Glyma11g04700   GTTGGAGTAAATCCAATAACATCAAATCCTTAATATATATTTATTAAATTTTATTGATAAAACTG
  gDNA + about   ACTTACTAGTACATATTTTAGTTTGTAATAATATCATTTGTTTGGATCCAATATATAAGCCAATT
  5 kb promoter   TTTTTTATGGACAAAATATATGGAGCCAAAGCCGCAGCTCAAAAACCTATGTAACAAGAGACACT
  and 5′UT   GAAGAGTGAAGAATCAGCAACATGATCAAAGCCTAAAATTGGGGCAAAAATTCAAACACTTGGCT
  sequence (SEQ   ATAAATACACCAGATAGTCCATACTTAGCCGCTATTATGTCAAAATATAATAGTATTAATATTAC
  ID   ATGGCAAAGTATAGGCTATATAATTTAATGTAATTTATTAAATTTTACAAGGTACTGATTCAACT
  NO: 32) Soybean   TTAAACATGTATGCTAATTGGAGTTTAAAATTTGTGAACAAAAAGCAAGTGCATTTTGTTGCGTG
  BAM2-like gene   ATCAAAATTGCTCAACCTTATCATGTAGGAAAACGGATAACCAGAATTTGTGTGGTCCCAAACGA
    CAACAAGACGCATTTATAAGCTTGACTAGTTCTCTTCGTCGTCAACTGACATTCTCATTTCTCAA
    TGATAGTTGCTACTTGATAATATTTTATTCGAATAATCTGTCGTTAACCTACCTATAATATATAG
    CTGGTGCTATTAATCGAATGTTTAATCTCATTTTAAGATTTACAGTGTGTGGATTGATGGTGAAG
    ATCCAAAAATCATAGTATCTGATTATGATTTAGTTTCCACCGCATCAGAGAGTATAGCTAGCTAG
    TTTTAAAGTTAGCATGATTTTTTCAAGATAACCCACCGTAGATTTTTTCAACATAATATAATATA
    ATTTTCACTTGTAAACTTTGAGGTTGCAAGGAAGAAAAGCAGGTAAAAAGAATAACAGGTAGCAA
    AGACATTTAAAAATTAAAATAGTTCTAACAATATAAGTCCAATCTAAAGGGGATACGTCCAGCAA
    TACTCATCCCTCACCAACTCCAACTTCACTCTCAATAAACTGGAATCGTGAAAGCATCATTACAA
    TTATCTCCTAGCTAACCAAACCCAACATTTTTTTTAGCTTTTAGAAATATTATCGCGTGCAATGT
    GATGCACTGCTGCAGTTAGCATCAACAAGAATAGTAACCTGACCCTTCATGCCATTATGATCGAG
    GTGGTAAAAAATGGCAAGTAGAAGTGAGGTTCATGCTCTTTAATGATTAATCTAATGGGATAACA
    AGAACCAGAACAAACAGAACTCTTGGTAGAAAAGAAAAAAAAAAGTGGGTCAATAATGCATATTT
    TGGATTCAAAACCACCACTGTCCAATTGACATCATTGTTCTACAAAACCGGAATGATTGTGATTC
    ATCCGGAGGGTATTTGCTCATTCATGTTCCTTATTATCGATATGGGCATACCTGACTAGCCAAGT
    ACAATTTCCTTAATTTCAATTTTGGCACTTACAATCGTGATTAAAACTGAGATCAGGTTTATATA
    TATGCTTGTCTTTTTATCCAAAAATTAGCATGCATTCTATATTTATGGGGTACGGGTCACGTGTG
    TACAATATACTCCTTACAAAAGGTTTATATATCTGCTTGGCTTTTAATCCCAAAATTAGCATGCA
    TTAAATGAAGGGTAACGTGTGTTTTATTCTTATTTAAATAAATAACATATAGTACAATTTTTAAG
    TAGCCAATAATTTTAAAATTTTCACTAACTCTGTATCTGTATTGTAATGAAAATATTTTTATATT
    TTACTTTTGGATCAATTTAAATTTATTTGTAAACAAATGGTTTTACATTTTATTAATTTCTTTTA
    TTAAATCTGTCCATAATATCTTTTTTTTTTATAAGTTTTAAATTTTATAATTTTAATTTAAATTT
    CTAATACAACGTAAGAGGATTAATATACTTAGCTAGTTAAAGATTATAATAATTATTTTCAACTG
    CGTTGGAGTTAGCTGGGATGACCACGGATCTTCCCCCCCATAAATTACCACAAAGCACCCCATTT
    GTTACACAGAAAGGGACTCTTGCAACAAGAGAATAAGGGACATTAAGTAATTTGCCTATTAATAA
    TGTTATAAGCTAATATAAAATTAGTTTGGCGGTTAAAATGAAAATTTAAAGATTGAAGGGAGAAA
    GAAGAAGAAAAGAGAGTTTTAAATTCAAATCTTCCACTGATCTTGGTTGATAAAAAAATGAAACC
    GCACACAAAAACGCTCTCCATCAATGCAATTGTACTAGTAATACTTAACTTGTGTCTTATATACA
    GCGTGGAAATATAAAATAAATAACATAATTATCATTTTTTGATAATATTATATATATATATATAT
    ATATATATAACTATTTTTTATATACGTTTGAGTACATAAGGAAACAATCTTGCTATTACCAATCT
    ATATTAGTTGTGGCTTTCCTCATAGAATTGATCCATGAAACGAAGGAGTAACACTGAATAATAAT
    AGTGCTAATGAAAAACCCATTATAATAGTAATTACTAATATTATTATGAAATATGAAATGTTAAT
    ATTCGGTGACTGCTTGGTCATTTTCTCTTCCAGAAAAACAGAGCTGTGACCTGTTAGTAAGGCCA
    TGGTGGGAGGGACCACTGCATGGCATCTTTCTCAGTGCTTCCCTTATTACATGATTTTGATGGCT
    TCAGTTGTCAGAGACCGGGTGGGTGGGTAGAAGATGGAGTATTGTATAGGAAGAAAATGGTAAAA
    TCATATTGAATCTTCTGCAATCCCCAATGTACTCTAGTTAGTAACTGTAATGTAAGGGCCTATTG
    TAATAATTGAAGCAGCACAGGGGCGAAGTCTCACATTCATAATAGGGTTTATCGAAAACACCACA
    CCATACCACTTGCCACGCCCCCTCTCTTTTCGTGACGGTCAACATTCTTTGACCACCTTTATCCA
    ACCTAACTAAATCATTACTACTGTTTATTAATTTATACTCTTGTTTTAATTTTCTATATTGAATT
    TCATTCATTTGTAATATTAATATAGGTGTGAAAATGACCATGATCAATAAAAAGAAAGGAAGAGC
    AATATCTAGCTTTTAGTGATAACATTGGACTTCTTTTTTGTTTTAACAAAAATTAAAGAAACAAC
    AGTCATTTTTCTGTCATAACTTGATGCCTTGACAAATTAATTTAATACTGTAAGATTATTGTAGA
    ACCCGTGATTATGCAGTAGAAGAACATAAATTTGTATGTTTCTCATCTAAGAAAGGAAAAGTAGC
    TAGAAAAAGAATGGTAGAGAGGGTGAAATAGCGAAATGCATGCTATGGCCAACGGGTTCCTTATT
    CCTTGCGGAGGCTATACAGTAGAATGGTTGTCCTAGATCACTTCAAATAGAATACGACACCCATG
    ATTGTCAAAAGGCTAAACAAGTTGATGCGCGCAATTTCCTTGTTTTCAGTCATAATTTTGGACTA
    AACTCCACACAGAACGACATGTTCTTTTCTAGGCTTTTGCTTTGTTGAATACTAGCGTTGGATTT
    TACTCTCTTCCTCACAAGATGGTAACAAGTTAGATAATCTATATAAGATTTCTGACCTTATTCGT
    CTTAATATAATAAACATGTTATAGCGAGTATATATCATGTGCTCCAATACATGCAGTTTTGGCAA
    TGGATTAGAAGTGTTAACGTTCCAGCAGAGTAATTCATACCATCCCCCATTTTTAATGCTCAAAA
    TGAGCAAGATGAAAATTTTTTAAACGTATCTTAATTCTTAGGGCATTCATTATTATCAAAAAGCC
    TTTATATTCATTAGAACTCTTTGCATGTATAGATCATTTTCTCTTTTTTTTTATTAAAAAAATTA
    ACATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATA
    TATATATATCAGGCCATTCATTAAGCAATTAAACATGAAAATATTTTATACAAAATATTATTATA
    TCTATTGTAAGTTTTAATTAAAAAATTCATTGATATTCTTAAAACGTTTGTTAGTAAAAAATATA
    TTTTAAATTTAAAAGGTTATATATAGTTATACATTATTATATTATTCTTAAAATTTAAAACTTAA
    TAATAATAAAATAAAAAATAGTATTCTTAATAACTAGACACGACAACAAAATAAATAAATAATTA
    AGACAAGGAAAACTAACAGAAGAATTAGCCGTTTTCCTCGACCTTCCTTGGAAAATAAGGCAATA
    GCATAGGACCTACTTAAAAAAAGTTAAAACATTCGACTACAAAAACATACAAAATGGACAAAGAT
    AAACACGTAAGAAAAACTAAGAAAAACGTTACATTTTTTTCTTTTCAATTTCACGTATTGTTATT
    GAAAATTTTATTTCAAACATTGTTCATTTATTTGTTTTTTTAAGAGAGTTCATTCATTTGTTATT
    AATTTAACAAATTATTTGTTAACGATCTATTTTAAAATTCAAAACCTATTTTTATTAAACTCATT
    AAATTATGTGCACCATTTTTTTTATTATAAATATAATAATAACTGTTATATAAATTTGATGAATG
    ACATGATAAAAGACCGTATTATTTGCATAATTAAAGAAGCACGCCATATTTATGTCTTTCCTATC
    ACAATCATAAGTAAAACTTGAGTTTACCACCATCCTCCGCTCAATAACCCAGCAACACACATAAC
    ATTCTTTTATTAATGTCATTTTTAAGTGGCATAATAACTATATAACAACACACATGAGTGCCGCA
    TCATAAATTACACATACGATAAATAAATCTTCATTATTATCTTATGCAATTATATATGTATTATG
    AGTGGTTCATTAAAAAATAGTGCAGCAAAGTCACCATAGCCGTGGGTGAATGATTGATAGGTAAA
    ATTGTATTTTTCTTTTTTTCCCGGGTATTTCAAAAAGTAAAAAGAGTTGAAGGGACGAATTCATA
    TATTCAGAAAATTCCCTCTCCTTTAAGTATCGGTTTGTGTTTGGGGGCATCACTCGTTGTTTCTC
  TCTTCCATGCCCAAAATGCGTGTCCTCTTTGTTTTTCTGTTTTTCCATTTTCATTTCCCTGAAAC
    CCTTTCTGCCCCAATCTCAGAGTACCGCGCCCTTCTCTCTCTCCGTTCAGTCATTACCGACGCCA
    CACCACCCGTTCTCTCTTCTTGGAACGCCTCCATCCCTTACTGTTCCTGGCTCGGCGTCACCTGC
    GACAACCGCCGCCACGTCACCGCCCTCAACCTCACCGGCCTCGACCTCTCCGGCACGCTCTCTGC
    CGACGTCGCCCACCTCCCTTTCCTCTCCAACCTCTCCCTCGCCGCAAACAAATTCTCCGGCCCCA
    TTCCTCCCTCTCTCTCCGCCCTCTCCGGCCTCCGCTACCTCAACCTCTCCAACAATGTCTTCAAC
    GAAACCTTCCCCTCGGAGCTTTGGCGCCTCCAGAGCCTCGAGGTCCTCGACCTCTACAACAACAA
    CATGACCGGCGTGCTCCCTCTTGCCGTCGCGCAGATGCAGAATCTTCGTCATTTGCATCTCGGCG
    GCAACTTCTTCTCCGGCCAGATCCCGCCGGAGTACGGACGCTGGCAGCGCCTCCAGTACCTCGCC
    GTCTCCGGCAACGAACTCGACGGGACTATCCCGCCGGAGATCGGAAACTTGACCAGCCTCCGGGA
    GCTCTACATCGGCTACTACAACACCTACACCGGCGGCATTCCGCCGGAGATCGGAAACTTGTCGG
    AGCTGGTGAGGCTTGACGTAGCGTACTGTGCGTTGTCCGGGGAGATTCCGGCGGCGCTTGGGAAG
    CTTCAGAAGCTGGACACGCTGTTCCTTCAGGTGAATGCATTGTCAGGATCACTGACGCCGGAGCT
    GGGGAACCTGAAGAGCCTGAAATCCATGGATTTGTCTAACAACATGCTCTCCGGTGAGATTCCGG
    CGAGTTTCGGCGAGCTGAAGAATATTACGCTTCTGAATCTGTTCAGGAACAAGCTTCATGGAGCT
    ATACCGGAGTTTATAGGAGAGCTTCCAGCGTTGGAAGTTGTGCAACTGTGGGAAAATAACTTAAC
    AGGTAGCATTCCTGAGGGTTTGGGCAAAAATGGGAGACTCAACCTTGTTGATCTTTCTTCTAACA
    AGTTAACCGGGACTTTGCCTCCTTATCTCTGTTCTGGGAATACTCTTCAGACTCTGATAACTCTT
    GGGAATTTTCTTTTCGGTCCAATTCCTGAGTCGCTCGGGACTTGTGAATCTCTTACACGGATTAG
    AATGGGAGAAAACTTTTTGAATGGTTCCATTCCTAAAGGGCTTTTTGGACTTCCCAAACTCACCC
    AGGTTGAACTTCAGGATAATTATCTCTCTGGAGAGTTTCCTGAGGTTGGTTCTGTTGCGGTTAAT
    CTTGGTCAGATTACTCTCTCTAACAACCAGCTTTCTGGGGCTCTGTCTCCCTCCATTGGTAACTT
    CTCCAGCGTGCAGAAGCTCCTTCTTGATGGCAACATGTTCACCGGTCGGATACCTACACAGATTG
    GGAGGTTGCAACAGCTTTCTAAGATTGATTTTAGTGGCAACAAGTTCTCGGGTCCTATTGCGCCT
    GAGATCAGTCAGTGTAAGCTGTTAACTTTCCTGGACCTTAGCCGCAATGAGCTATCTGGAGACAT
    CCCTAATGAGATAACTGGCATGAGGATATTGAATTACTTGAATCTTTCTAAGAATCATTTAGTGG
    GTAGCATTCCCTCTTCGATATCATCTATGCAAAGCTTGACTTCTGTTGATTTTTCATACAACAAC
    CTGTCTGGTTTGGTGCCTGGTACCGGTCAATTCAGCTACTTCAACTACACGTCTTTCTTGGGAAA
    CCCTGACCTGTGTGGCCCCTATTTGGGTGCTTGCAAGGGTGGGGTTGCCAATGGTGCACACCAAC
    CTCATGTTAAAGGACTCTCCTCTTCTTTGAAGCTGCTACTTGTTGTTGGGTTGCTATTATGTTCC
    ATTGCTTTTGCTGTGGCTGCAATATTCAAGGCCCGGTCATTAAAGAAGGCCAGTGAGGCTCGTGC
    ATGGAAGTTGACTGCGTTCCAGCGTTTGGACTTCACTGTTGATGATGTTTTGCATTGCTTGAAAG
    AGGATAATATTATTGGGAAAGGAGGTGCTGGAATTGTCTACAAAGGGGCTATGCCTAATGGGGAT
    CATGTTGCTGTGAAAAGGCTTCCAGCTATGAGTAGAGGCTCTTCCCATGATCACGGATTCAATGC
    TGAGATTCAGACATTGGGGCGAATCCGACACAGGCACATTGTTAGGTTGTTGGGTTTCTGTTCAA
    ATCATGAGACAAACCTTTTGGTCTATGAGTACATGCCCAATGGAAGTTTAGGTGAGGTTCTTCAT
    GGAAAAAAGGGGGGTCATTTGCATTGGGACACCAGGTATAAAATTGCGGTGGAGGCTGCCAAGGG
    GCTTTGCTATCTGCACCATGATTGTTCGCCACTCATTGTCCATCGTGATGTGAAGTCAAACAACA
    TCCTTCTTGATTCAAATCATGAAGCCCATGTTGCTGATTTTGGGCTTGCTAAGTTCCTGCAAGAT
    TCTGGGACATCTGAATGCATGTCTGCTATTGCTGGTTCATATGGATACATAGCTCCAGGTACCGT
    TGAATTTTGACATAATTAATGCATCATATGCATGGTTGTGGCAAATTTCCTTTTTCTCGCCTAAT
    CATAATTGTACGTTTAAGCATTTTGTTCAGAATTTGACTCTTTGACTTATGCATGATATTGAGGT
    GATGCCCCTAAATTTATTAACATTGCTATGTGGTTTTTCTTGACTTTGGTTTTCTATCATACCCA
    ATTGATTCGCCCCCTTATTTTGTTTTTTTTTCTAAGCCAAGTACTGAAAGTAAATGGTAGGTATC
    TCTGCACCGTTTGATTTTTTACCCTAACCCCCTCTCCCCACCTATGAAGTAGATAATGCTGTAGT
    CGTAGGTTAAGAGTCATTCACAATCGGAAACTGATGGTTATGGGCAAAAACATCAGATAAAAAGA
    CCTATTATGTTACTTTATACGTATTGCCTTTGTTTAACTTATTGTTTCAAATTAAAGTGTCTTGC
    TTTATTATAGTGTATGATACCTGTTGGATGTTTGATTGCAGAGTATGCCTACACATTGAAAGTTG
    ATGAGAAAAGCGATGTGTACAGTTTTGGTGTGGTTCTTTTAGAACTTATAACAGGCAGGAAACCA
    GTTGGTGAATTTGGTGATGGCGTGGACATCGTGCAATGGGTGAGGAAAATGACGGACTCTAACAA
    GGAAGGAGTTCTTAAAGTTCTTGATCCTAGGCTTCCCTCAGTTCCCCTTCACGAAGTGATGCATG
    TTTTCTATGTGGCCATGCTGTGTGTTGAAGAACAGGCTGTAGAGAGACCAACAATGCGTGAAGTT
    GTTCAAATACTGACCGAGCTTCCAAAGCCACCTGGCTCTAAAGAGGGAGACTTAACAATAACAGA
    ATCCTCTTTGTCATCATCAAACGCTTTAGAATCTCCATCCTCAGCCTCCAAGGAAGATCAAAATC
  CTCCTCAATCCCCACCACCCGACCTTCTTAGTATTTAAAGTGCTCTGTTGGGTGTTTCATCTTAT
    TAGTTCCCTTGGTTGTGATAGCTTATCCATTTACTTTCTTTTTCTGTCTCTCTTCTGGGGTTGGG
    GCTTTTCTTCTTCTTCTAACTGAAGGTATTAATGCTCTGATTTTTTAATGGTTTTGTACAGTAGG
    ATTGGTGGGGGGGGTTATTTTCTTATGAAGTCACTTTCTTCATCATGTAGTACTGCTTTTTAATT
    TTTATGTTACGGCCGTTGTTGTGCTTCGCCTAAGCTGGGGAGTGGGGAGGGTTCAAGGGAATGGA
    TACTCTTTTTTTATGCGATCACTGACAGGTAGACACAAAATGACGCAAACGGGTTGGGTATTAAA
    CAGTGGGTATATTGTATGGTTTAGAATATTATTGATGAATCCTGAGTGGATTGGCACAGTGTGAA
    CTGTGAGCCTGAGCTGTGACTGAGTCTATGAGTCAGGTTTGGATAAAAGCTTATTTGAAGAAGTT
    AACCTGTTTCGAGAAAATCAGAGTGAATCAGGATTCAGGCGTGTTTTAGCTTT
 
  Glyma11g04700ATGCCCAAAATGCGTGTCCTCTTTGTTTTTCTGTTTTTCCATTTTCATTTCCCTGAAACCCTTTC
  cDNA (SEQ ID   TGCCCCAATCTCAGAGTACCGCGCCCTTCTCTCTCTCCGTTCAGTCATTACCGACGCCACACCAC
  NO: 33) Soybean   CCGTTCTCTCTTCTTGGAACGCCTCCATCCCTTACTGTTCCTGGCTCGGCGTCACCTGCGACAAC
  BAM2-like gene   CGCCGCCACGTCACCGCCCTCAACCTCACCGGCCTCGACCTCTCCGGCACGCTCTCTGCCGACGT
    CGCCCACCTCCCTTTCCTCTCCAACCTCTCCCTCGCCGCAAACAAATTCTCCGGCCCCATTCCTC
    CCTCTCTCTCCGCCCTCTCCGGCCTCCGCTACCTCAACCTCTCCAACAATGTCTTCAACGAAACC
    TTCCCCTCGGAGCTTTGGCGCCTCCAGAGCCTCGAGGTCCTCGACCTCTACAACAACAACATGAC
    CGGCGTGCTCCCTCTTGCCGTCGCGCAGATGCAGAATCTTCGTCATTTGCATCTCGGCGGCAACT
    TCTTCTCCGGCCAGATCCCGCCGGAGTACGGACGCTGGCAGCGCCTCCAGTACCTCGCCGTCTCC
    GGCAACGAACTCGACGGGACTATCCCGCCGGAGATCGGAAACTTGACCAGCCTCCGGGAGCTCTA
    CATCGGCTACTACAACACCTACACCGGCGGCATTCCGCCGGAGATCGGAAACTTGTCGGAGCTGG
    TGAGGCTTGACGTAGCGTACTGTGCGTTGTCCGGGGAGATTCCGGCGGCGCTTGGGAAGCTTCAG
    AAGCTGGACACGCTGTTCCTTCAGGTGAATGCATTGTCAGGATCACTGACGCCGGAGCTGGGGAA
    CCTGAAGAGCCTGAAATCCATGGATTTGTCTAACAACATGCTCTCCGGTGAGATTCCGGCGAGTT
    TCGGCGAGCTGAAGAATATTACGCTTCTGAATCTGTTCAGGAACAAGCTTCATGGAGCTATACCG
    GAGTTTATAGGAGAGCTTCCAGCGTTGGAAGTTGTGCAACTGTGGGAAAATAACTTAACAGGTAG
    CATTCCTGAGGGTTTGGGCAAAAATGGGAGACTCAACCTTGTTGATCTTTCTTCTAACAAGTTAA
    CCGGGACTTTGCCTCCTTATCTCTGTTCTGGGAATACTCTTCAGACTCTGATAACTCTTGGGAAT
    TTTCTTTTCGGTCCAATTCCTGAGTCGCTCGGGACTTGTGAATCTCTTACACGGATTAGAATGGG
    AGAAAACTTTTTGAATGGTTCCATTCCTAAAGGGCTTTTTGGACTTCCCAAACTCACCCAGGTTG
    AACTTCAGGATAATTATCTCTCTGGAGAGTTTCCTGAGGTTGGTTCTGTTGCGGTTAATCTTGGT
    CAGATTACTCTCTCTAACAACCAGCTTTCTGGGGCTCTGTCTCCCTCCATTGGTAACTTCTCCAG
    CGTGCAGAAGCTCCTTCTTGATGGCAACATGTTCACCGGTCGGATACCTACACAGATTGGGAGGT
    TGCAACAGCTTTCTAAGATTGATTTTAGTGGCAACAAGTTCTCGGGTCCTATTGCGCCTGAGATC
    AGTCAGTGTAAGCTGTTAACTTTCCTGGACCTTAGCCGCAATGAGCTATCTGGAGACATCCCTAA
    TGAGATAACTGGCATGAGGATATTGAATTACTTGAATCTTTCTAAGAATCATTTAGTGGGTAGCA
    TTCCCTCTTCGATATCATCTATGCAAAGCTTGACTTCTGTTGATTTTTCATACAACAACCTGTCT
    GGTTTGGTGCCTGGTACCGGTCAATTCAGCTACTTCAACTACACGTCTTTCTTGGGAAACCCTGA
    CCTGTGTGGCCCCTATTTGGGTGCTTGCAAGGGTGGGGTTGCCAATGGTGCACACCAACCTCATG
    TTAAAGGACTCTCCTCTTCTTTGAAGCTGCTACTTGTTGTTGGGTTGCTATTATGTTCCATTGCT
    TTTGCTGTGGCTGCAATATTCAAGGCCCGGTCATTAAAGAAGGCCAGTGAGGCTCGTGCATGGAA
    GTTGACTGCGTTCCAGCGTTTGGACTTCACTGTTGATGATGTTTTGCATTGCTTGAAAGAGGATA
    ATATTATTGGGAAAGGAGGTGCTGGAATTGTCTACAAAGGGGCTATGCCTAATGGGGATCATGTT
    GCTGTGAAAAGGCTTCCAGCTATGAGTAGAGGCTCTTCCCATGATCACGGATTCAATGCTGAGAT
    TCAGACATTGGGGCGAATCCGACACAGGCACATTGTTAGGTTGTTGGGTTTCTGTTCAAATCATG
    AGACAAACCTTTTGGTCTATGAGTACATGCCCAATGGAAGTTTAGGTGAGGTTCTTCATGGAAAA
    AAGGGGGGTCATTTGCATTGGGACACCAGGTATAAAATTGCGGTGGAGGCTGCCAAGGGGCTTTG
    CTATCTGCACCATGATTGTTCGCCACTCATTGTCCATCGTGATGTGAAGTCAAACAACATCCTTC
    TTGATTCAAATCATGAAGCCCATGTTGCTGATTTTGGGCTTGCTAAGTTCCTGCAAGATTCTGGG
    ACATCTGAATGCATGTCTGCTATTGCTGGTTCATATGGATACATAGCTCCAGAGTATGCCTACAC
    ATTGAAAGTTGATGAGAAAAGCGATGTGTACAGTTTTGGTGTGGTTCTTTTAGAACTTATAACAG
    GCAGGAAACCAGTTGGTGAATTTGGTGATGGCGTGGACATCGTGCAATGGGTGAGGAAAATGACG
    GACTCTAACAAGGAAGGAGTTCTTAAAGTTCTTGATCCTAGGCTTCCCTCAGTTCCCCTTCACGA
    AGTGATGCATGTTTTCTATGTGGCCATGCTGTGTGTTGAAGAACAGGCTGTAGAGAGACCAACAA
    TGCGTGAAGTTGTTCAAATACTGACCGAGCTTCCAAAGCCACCTGGCTCTAAAGAGGGAGACTTA
    ACAATAACAGAATCCTCTTTGTCATCATCAAACGCTTTAGAATCTCCATCCTCAGCCTCCAAGGA
  AGATCAAAATCCTCCTCAATCCCCACCACCCGACCTTCTTAGTATTTAA
 
  Glyma11g04700   MPKMRVLFVFLFFHFHFPETLSAPISEYRALLSLRSVITDATPPVLSSWNASIPYCSWLGVTCDN
  protein (SEQ   RRHVTALNLTGLDLSGTLSADVAHLPFLSNLSLAANKFSGPIPPSLSALSGLRYLNLSNNVFNET
  ID   FPSELWRLQSLEVLDLYNNNMTGVLPLAVAQMQNLRHLHLGGNFFSGQIPPEYGRWQRLQYLAVS
  NO: 34) Soybean   GNELDGTIPPEIGNLTSLRELYIGYYNTYTGGIPPEIGNLSELVRLDVAYCALSGEIPAALGKLQ
  BAM2-like gene   KLDTLFLQVNALSGSLTPELGNLKSLKSMDLSNNMLSGEIPASFGELKNITLLNLFRNKLHGAIP
    EFIGELPALEVVQLWENNLTGSIPEGLGKNGRLNLVDLSSNKLTGTLPPYLCSGNTLQTLITLGN
    FLFGPIPESLGTCESLTRIRMGENFLNGSIPKGLFGLPKLTQVELQDNYLSGEFPEVGSVAVNLG
    QITLSNNQLSGALSPSIGNFSSVQKLLLDGNMFTGRIPTQIGRLQQLSKIDFSGNKFSGPTAPEI
    SQCKLLTFLDLSRNELSGDIPNEITGMRILNYLNLSKNHLVGSIPSSISSMQSLTSVDFSYNNLS
    GLVPGTGQFSYFNYTSFLGNPDLCGPYLGACKGGVANGAHQPHVKGLSSSLKLLLVVGLLLCSIA
    FAVAAIFKARSLKKASEARAWKLTAFQRLDFTVDDVLHCLKEDNIIGKGGAGIVYKGAMPNGDHV
    AVKRLPAMSRGSSHDHGFNAEIQTLGRIRHRHIVRLLGFCSNHETNLLVYEYMPNGSLGEVLHGK
    KGGHLHWDTRYKIAVEAAKGLCYLHHDCSPLIVHRDVKSNNILLDSNHEAHVADFGLAKFLQDSG
    TSECMSATAGSYGYIAPEYAYTLKVDEKSDVYSFGVVLLELITGRKPVGEFGDGVDIVQWVRKMT
    DSNKEGVLKVLDPRLPSVPLHEVMHVFYVAMLCVEEQAVERPTMREVVQILTELPKPPGSKEGDL
    TITESSLSSSNALESPSSASKEDQNPPQSPPPDLLSI
 
  Glyma09g38720   CACGTGGTACACGAACACCGACGCCATCAGAATCCAAAAGGGTATCAGGAATCACAATCAAAAAC
  gDNA + about   GAATTTTGTTCTAGTTTTTATATCCTTAAAAAATTCGAAACCAGAGAGAGAAAAAAAATGGTTGG
  1 kb of   GTTTTTTTACTCTTGTCGGGTGAGAGCTATAAGAGGGTGTGGAGGAAGATGAGGAGAAGATCGAG
  promoter and   GGCGGTGATGGGATGGCGGTGGAGGATCACAGCAGAGAAATAGTTTGCCATTGCCATGGAGGGAG
  5′UT sequence   AGCGAAGAGGTTGAGGCCCATTCAATTGAATTGGATCAGAGAGAGTTAACTGAAGAATCGGTCAC
  (SEQ ID   TGAGAAAAGGGCGCGTAGCTTAGCATTTGATATGTGGCGATTTGGTTTGGGTACGTCCTTTCGGG
  NO: 35) Soybean   GACAGAAGAAGATGGATCAAAGACGCTTAATGCGGTTGGGACCTGAGAATGAATGAGAGAGACAC
  CLV2-1ike gene   TCACTACACTCACAAAAGGAGGTTCAATTTATCAAATAAAAAAGAGAGACACAGGGGATGGATGT
    GTCATGTGTGTGTCCATGTGTGGTGAGCTCCATCATATAGAGAATCTTTTCACCTTAATTATTTT
    TTAAGGCTATTCTTAATCAGTAATCTTAGACATTGATTAAAAAATTAAAAAGAAAATATAAAATA
    AGTTGTAGAGCACTATAATTTAATATTTTAATATAAAAAGTATTTAGAAGAATGATAAATATATC
    TAGCTTTCTTAATATATAAAATTAATATAAATTAGTATAATATCACAAATATTTTATTAAACCAA
    ACAATTAACATTTTAAAAATTTTATATTTGATTTTTACTGTGTCTAAAATTTTTTGGGTCGCTGA
    TAACCACAAATTACAAACAAAATTAATCTCCCATTGAATTAAAAAATAACATAATCTATAACCTA
    TCAAAAAGAAAAAGAAAAAAGAATCTGGACCTATTTCTACCCCGATGCACATGAGAAACTTAAAA
    AGGGGGTGAAGTGTTATGTAGTATAGAGAGAAAGCGAGGGAAGGCAAAGCAAGCACAACAGAACA
    AAGCCACTTTATTTTTTTGATCTAACCTAAACCATCCTTTCCCCCTGTTGCACTCTCACTTTATC
    AACGTGACACAAGCAACTTATGACCAATGTGTAAGATGTTGTTCCTCTTTCCCTTCTCTTCTGTC
    CATTTCATCAAGTTTCCATTCTAATCTCCAAATCTTTGCCACCCCAGTTCCTCTTTTGCTTCAAA
  CTTCTCTTCCCCTCCCTAAAAATTGCACCTTTACTCTCATGGTGATGGGACACACCACACCCCTC
    ACACTCCTCTGTATGATTCTTCTTTTTGCAACCCCTTCTCTCTCAATTGATGTTCACCCACAAGA
    CAGAATCTCACTCTCACTGTTCAGGTCATCTCTGCCAAACCCCAACCAGAGTTTGCCCAGCTGGG
    TAGGCTCCAACTGCACTTCATGGAGTGGAATCACCTGCGACAGCAGAACTGGGAGAGTGCTTTCC
    ATCAACCTAACTAGCATGAACCTTTCAGGCAAAATCCACCCCAGTTTGTGCCACCTTTCATACCT
    CAACAAGTTGGGGTTGTCACACAACAACTTCACAGCCCCACTTCCTGAGTGTTTTGGAAACTTGC
    TTAACCTAAGAGCCATTGATCTCAGCCACAACAGGTTTCATGGTGGAATACCAGACTCTTTCATG
    AGGCTCAGGCACCTCACTGAGCTTGTTTTCAGTGGGAACCCTGGTTTGGGGGGTCCACTTCCTGC
    TTGGATTGGTAACTTCTCTGCAAATCTGGAAAAGTTACATCTTGGTTTCTGTTCATTCAGTGGTG
    GCATACCTGAGAGCTTGCTTTACATGAAGTCCCTCAAGTATTTGGACCTTGAGAACAATCTCTTG
    TTTGGTAATTTGGTTGATTTTCAACAGCCTTTGGTTTTGCTCAATCTTGCTTCCAATCAGTTTGC
    TGGTACTTTGCCTTGCTTTGCAGCTTCAGTTCAGTCTCTAACTGTGTTGAATTTGTCCAACAATT
    CTATTGCGGGGGGATTGCCTGCTTGTATTGCTTCTTTTCAAGCTTTGACTCATTTGAACCTTTCA
    GGGAACCATTTGAAGTATAGAATATATCCTAGGCTTGTGTTCTCAGAGAAACTTCTTGTTTTGGA
    CTTGAGTAATAATGCTTTATCTGGTCCTATTCCCAGTAAAATTGCTGAGACTACTGACAAACTTG
    GCCTTGTTCTTCTTGACCTTTCTCACAATCAGTTCTCTGGTGAAATACCTGTGAAAATTACTGAG
    TTGAAAAGCTTGCAGGCCTTGTTTCTCTCTCACAATCTTCTCTCAGGAGAAATTCCTGCTAGAAT
    TGGAAATTTGACTTATCTGCAGGTCATTGATCTCTCACACAACTCTTTGTCTGGAACCATTCCAT
    TCAGTATTGTTGGGTGCTTTCAGCTGTATGCTCTGATACTTAACAACAACAATCTTTCTGGTGTA
    ATTCAACCGGAGTTTGATGCGTTGGATATCTTGAGGATACTGGATATAAGCAACAACAGGTTTTC
    CGGGGCTATCCCACTCACTTTGGCTGGATGCAAATCTTTGGAGATTGTAGACTTTAGTTCCAATG
    AGCTTTCTGGATCGTTGAATGATGCAATAACCAAATGGACAAACCTCAGGTATTTGTCTCTTGCT
    CAGAACAAGTTCAGTGAAAATCTGCCTAGTTGGTTGTTCACATTTAACGCAATAGAAATGATGGA
    TTTCTCGCATAACAAGTTTACTGGCTTCATACCGGATATTAATTTTAAGGGTAGCTTAATATTTA
    ACACTAGGAATGTCACTGTTAAAGAGCCATTGGTTGCAGCAAGAAAGGTTCAACTCAGAGTTTCG
    GCGGTTGTTTCTGATAGCAATCAACTCAGTTTCACTTATGATCTTTCCTCAATGGTTGGAATTGA
    TCTATCCAGCAACTCGCTTCATGGGGAAATTCCAAGGGGCTTATTTGGTCTATCTGGCCTAGAAT
    ATCTGAATTTGTCATGCAACTTTCTTTACGGACAGCTTCCGGGGTTGCAGAAAATGCAGAGTTTG
    AAAGCCTTGGATTTGTCACATAATTCCTTGTCAGGACATATCCCAGGAAACATCTCTATCCTTCA
    AGATCTGTCTATTTTGAATCTTTCCTACAACTGCTTTTCTGGATGTGTTCCCCAGAAGCAAGGGT
    ATGGGAGATTTCCTGGTGCATTTGCTGGAAATCCAGATCTGTGCATGGAATCTTCCAGTGGATTA
    TGTGATGATGGAAGGACTCAATCTGCGCAAGGAAGTACTTTTAGGGAAGATAGGATGGATGACCC
    AATTTCTGTGGGGATTTTCTTTATCAGTGCATTTGTTAGTTTTGATTTTGGTGTTGTGGTTCTCT
  TCTGTTCCGCACGGGCAAGAAATTACATTCTCCAAACAAAAGTTTGATTTGATGCTTGTGACACA
    TACAAATCTCCTGTAAATTCCATTTTGTAATGTGGTACCTGTCTTCTCAGTTTCAAGTAAACATA
    CACTTACGTGACTGGGAATACTATCTGGCCATCAGCTTCACAAGTGTTTTCTCGTGATTACTGAA
    CAAGTGTCTCGGAATTGCAGGATCAAAATGCCATGATATGAGTAACACAAGGTTTAAAGAACACT
    CATAACGCTGGCTTTAACTATCTGAGTGAAGACTAGTCCTGCATCATTCAGCCAAGAAAAAAATG
    GATGGTTATGATGAAAATTTGATCCAAGTAAAGACGAGTCCCTCATCATTCTGATGGTTGTTCTC
    TTTTGCTGGAACTTGGTTGCATCAAGTTTATTATGCATCATCACATGCATTATTCATAATCAGGT
    GGGTGAAGGGTCAGCAAGGAACATGCCTGATTGATATCTGGTCTAGTTATGGTGAAATTTTGATC
    TTGGGACATCAAATTGCAGATTTGCAAGCATGTTTACGTGAAGAGAACTTGTATCATTCTAGATT
    AACCCAGCTCTTTCTTGAGGTGGGGAACCAAGTTTTCCCTGTAAGTGTTTTACCTTAAGAATGTG
    AGTTGATGAGTAGTGGGGAGTGGTAAGTGCAGACAAAATAAATGGAGTAGTTCTCATAAATCTAA
    GATTTGTATTTGTATTACTGTCTTCATGCCTTCATCTTAGTGCTGTGATTTTAAATGAAATTCTC
    ACGAAATCTTTTCATTGAGAACAGAAAAGAGGTAATTGAGCACCTTAGCTTTGTTATCAAATGCC
    AAGCATGCTCAACAAAAATTAGAAAAATTATCTAGTTTACCAA
 
  Glyma09g38720ATGGTGATGGGACACACCACACCCCTCACACTCCTCTGTATGATTCTTCTTTTTGCAACCCCTTC
  cDNA (SEQ ID   TCTCTCAATTGATGTTCACCCACAAGACAGAATCTCACTCTCACTGTTCAGGTCATCTCTGCCAA
  NO: 36) Soybean   ACCCCAACCAGAGTTTGCCCAGCTGGGTAGGCTCCAACTGCACTTCATGGAGTGGAATCACCTGC
  CLV2-like gene   GACAGCAGAACTGGGAGAGTGCTTTCCATCAACCTAACTAGCATGAACCTTTCAGGCAAAATCCA
    CCCCAGTTTGTGCCACCTTTCATACCTCAACAAGTTGGGGTTGTCACACAACAACTTCACAGCCC
    CACTTCCTGAGTGTTTTGGAAACTTGCTTAACCTAAGAGCCATTGATCTCAGCCACAACAGGTTT
    CATGGTGGAATACCAGACTCTTTCATGAGGCTCAGGCACCTCACTGAGCTTGTTTTCAGTGGGAA
    CCCTGGTTTGGGGGGTCCACTTCCTGCTTGGATTGGTAACTTCTCTGCAAATCTGGAAAAGTTAC
    ATCTTGGTTTCTGTTCATTCAGTGGTGGCATACCTGAGAGCTTGCTTTACATGAAGTCCCTCAAG
    TATTTGGACCTTGAGAACAATCTCTTGTTTGGTAATTTGGTTGATTTTCAACAGCCTTTGGTTTT
    GCTCAATCTTGCTTCCAATCAGTTTGCTGGTACTTTGCCTTGCTTTGCAGCTTCAGTTCAGTCTC
    TAACTGTGTTGAATTTGTCCAACAATTCTATTGCGGGGGGATTGCCTGCTTGTATTGCTTCTTTT
    CAAGCTTTGACTCATTTGAACCTTTCAGGGAACCATTTGAAGTATAGAATATATCCTAGGCTTGT
    GTTCTCAGAGAAACTTCTTGTTTTGGACTTGAGTAATAATGCTTTATCTGGTCCTATTCCCAGTA
    AAATTGCTGAGACTACTGACAAACTTGGCCTTGTTCTTCTTGACCTTTCTCACAATCAGTTCTCT
    GGTGAAATACCTGTGAAAATTACTGAGTTGAAAAGCTTGCAGGCCTTGTTTCTCTCTCACAATCT
    TCTCTCAGGAGAAATTCCTGCTAGAATTGGAAATTTGACTTATCTGCAGGTCATTGATCTCTCAC
    ACAACTCTTTGTCTGGAACCATTCCATTCAGTATTGTTGGGTGCTTTCAGCTGTATGCTCTGATA
    CTTAACAACAACAATCTTTCTGGTGTAATTCAACCGGAGTTTGATGCGTTGGATATCTTGAGGAT
    ACTGGATATAAGCAACAACAGGTTTTCCGGGGCTATCCCACTCACTTTGGCTGGATGCAAATCTT
    TGGAGATTGTAGACTTTAGTTCCAATGAGCTTTCTGGATCGTTGAATGATGCAATAACCAAATGG
    ACAAACCTCAGGTATTTGTCTCTTGCTCAGAACAAGTTCAGTGAAAATCTGCCTAGTTGGTTGTT
    CACATTTAACGCAATAGAAATGATGGATTTCTCGCATAACAAGTTTACTGGCTTCATACCGGATA
    TTAATTTTAAGGGTAGCTTAATATTTAACACTAGGAATGTCACTGTTAAAGAGCCATTGGTTGCA
    GCAAGAAAGGTTCAACTCAGAGTTTCGGCGGTTGTTTCTGATAGCAATCAACTCAGTTTCACTTA
    TGATCTTTCCTCAATGGTTGGAATTGATCTATCCAGCAACTCGCTTCATGGGGAAATTCCAAGGG
    GCTTATTTGGTCTATCTGGCCTAGAATATCTGAATTTGTCATGCAACTTTCTTTACGGACAGCTT
    CCGGGGTTGCAGAAAATGCAGAGTTTGAAAGCCTTGGATTTGTCACATAATTCCTTGTCAGGACA
    TATCCCAGGAAACATCTCTATCCTTCAAGATCTGTCTATTTTGAATCTTTCCTACAACTGCTTTT
    CTGGATGTGTTCCCCAGAAGCAAGGGTATGGGAGATTTCCTGGTGCATTTGCTGGAAATCCAGAT
    CTGTGCATGGAATCTTCCAGTGGATTATGTGATGATGGAAGGACTCAATCTGCGCAAGGAAGTAC
    TTTTAGGGAAGATAGGATGGATGACCCAATTTCTGTGGGGATTTTCTTTATCAGTGCATTTGTTA
    GTTTTGATTTTGGTGTTGTGGTTCTCTTCTGTTCCGCACGGGCAAGAAATTACATTCTCCAAACA
  AAAGTTTGA
 
  Glyma09g38720   MVMGHTTPLTLLCMILLFATPSLSIDVHPQDRISLSLFRSSLPNPNQSLPSWVGSNCTSWSGITC
  protein (SEQ   DSRTGRVLSINLTSMNLSGKIHPSLCHLSYLNKLGLSHNNFTAPLPECFGNLLNLRAIDLSHNRF
  ID   HGGIPDSFMRLRHLTELVFSGNPGLGGPLPAWIGNFSANLEKLHLGFCSFSGGIPESLLYMKSLK
  NO: 37) Soybean   YLDLENNLLFGNLVDFQQPLVLLNLASNQFAGTLPCFAASVQSLTVLNLSNNSIAGGLPACIASF
  CLV2-like gene   QALTHLNLSGNHLKYRIYPRLVFSEKLLVLDLSNNALSGPIPSKIAETTDKLGLVLLDLSHNQFS
    GEIPVKITELKSLQALFLSHNLLSGEIPARIGNLTYLQVIDLSHNSLSGTIPFSIVGCFQLYALI
    LNNNNLSGVIQPEFDALDILRILDISNNRFSGAIPLTLAGCKSLEIVDFSSNELSGSLNDAITKW
    TNLRYLSLAQNKFSENLPSWLFTFNAIEMMDFSHNKFTGFIPDINFKGSLIFNTRNVTVKEPLVA
    ARKVQLRVSAVVSDSNQLSFTYDLSSMVGIDLSSNSLHGEIPRGLFGLSGLEYLNLSCNFLYGQL
    PGLQKMQSLKALDLSHNSLSGHIPGNISILQDLSILNLSYNCFSGCVPQKQGYGRFPGAFAGNPD
    LCMESSSGLCDDGRTQSAQGSTFREDRMDDPISVGIFFISAFVSFDFGVVVLFCSARARNYILQT
    KV
 
  GmNARK:   GCACCCACTGGGTAAGTTGGTAACTACTATGTATCTATATATCGTCAGGTCATTGTCTGTTTCAT
  Glyma12g04390   TCTCTTCTCACAAGAACAAAATGGTAATTTACATTTAACTTAGAAATGTTTGGGACAGAACCTCT
  gDNA + 5 kb   AGCTTGCGATGATTCTCTTCTCACAAGAACAAAATGGTAATTTACATTTAACTTTAGAAATGTTT
  promoter and   GGGACCGAACCTCTAGCTTGCGATGATTCTCTTCTCACAAGAACAAAATGGTAATTTACATTTAA
  5′UT (SEQ ID   CTTTAGAAATGTTTGGGACCGAACCACTAGCTTGCGATGATTCCCTTCTCACAAGAACAAAATGG
  NO: 38) Soybean   TAATTTACATTTAACTTAGAAATGTTTGGGACAGAACCACTGGCTTGCGATGATTCTCTTCTCAC
  CLV1-like gene   AAGAACAAAATGGTAATTTGCATTTAACTTAGAAATGATTGGGACAGAACCACTAGCTTCGATGA
    ATAATTTGCTTTAATTTTTATTAATGCATAATACCCTTTTATTGTCACACATAGAATCCGATTCT
    GCAATAACTAGTGCTTGATCCTAATTGACAGAACAAATTAAAACAGAGAATTGATGCTTTGGCTT
    TTCCATGGGCAATAATTATCCCAATGATATACTAAAGCATAGTAACTAGGAAGACTTCCATGTAA
    AGAAACTTTCTTTTATTCTCCTTTTAAAATTTGGTGAATCACTTAAAACCACTTTTGTTTCATTC
    CAAGGTTAGGCTCATGGAAAGCTTAAACCTACTTAACTGGTCACGAAGAGATTGCATCTTTGTTT
    TCACAAAAGTCTAACTCCAAGTTCGTGTAGCTAGTATTGCATGCTACCATGGTGCAAGTGATGTA
    CATGCATATATGATATTCAATTTAATTTGCTACTATAATATAAAGGTGTATATATAAATAGAGAG
    TGCATGAGGTGTGTGGTGTCAACATATAAGGACGCAGCAAAGGTATAATAGCGACTACTGCGAAG
    CAAGATCAGAGACTAGAGAGACATGATAAGAAGTTGTTAATTTGTTTTCTTCATATGGCTGCGCG
    TGGCAACGTGCTCTTCGTTCACTGACATGGATGCGCTGCTGAAGCTGAAGGACTCCATGACTGGA
    AGTTTTCCACGTCGCTTTCTGCACACTGTTTCTTTTCAGGCGTAAACTGCGACCAAGAACTTCGA
    GTCGTTGCTATCAATGTCTCGTTTGTTCCTCTTTTCGGCTACCTTCCGCCGGAGATCGGACAATT
    GGACAAACTCGAGAACCTCACTTCCCTCAAGCTCCTCGACATCTCTCACAACGTCTTCTCCGGCC
    AAATTATTCTTCCGATGACGAAACTGGAGGTCCTCGACGTCTACGACAACAACTTCCGGCAGCAT
    ACCGGAGATTTACTCGGAGTTTAAGAGCTTGGAGTTTTTAAGCTTAAGCACCAATAACTTATCGG
    GGAAGATTCCGAAGAGTTTGTCTAAGTTGAAGACGCTGAGGTATCTCAAACTCGGATACAACAAC
    GCTTACGAAGGTGAAATTCCACCGGAGTTTGGCAGCATAAAATCTGAGATACCTTGACCTCATCG
    GCGAGATTCCACCTACTCTAAACAATAATAAGAAAAACTTATCACATTTCTTGAAACTTTAAAAG
    ACCGATAAAAATAAAAGGAGGAAATGCCACTACAATATTTTTAATTTATTTTTTTTACTTATTTT
    ATTTGAATCTTTAATACATATGCTATTTTAGCATTATAAAAATACCTGGGCTATACAAAATATAC
    TTGCTAGTAGTATTATGTGTGTGTGAAAGTTAAATGAGTCTTTAAGTATTTGTAAATGTTTAATA
    AGTTTCGAGGTTTATCTTGATTCCAACAATGAATTCCTGAAATCTAATTTATCTAACTTTTTTTT
    AACCAAAATGTTAAATGGTCTAGTTAAGAGAACAAATCCTTATGTGTTCATTTTTTCACAAGACC
    TAAAATCTAAAATTTCACTTTAAAAGAAACAAAATACTTGCTACTTGAACTAACAATCATTAGTA
    CATTTTTTTAGTAATGATATACAAACATCTAAAACTCCTATACAACACAACACATAGAAGACAAT
    AAAAAATATCAATATGATAAATAAAAATGAGAAATAGATGAATTATTTAAAATAATGAAATGTTT
    ATTTATCATTACTTTTTTTTACTTTAACAGTTCATACATCTCCTACAAGGTAAGATGTGTAATGC
    AAGTAAGTTGCAACATGGTTTTAAATTTTGACAATAAGAACCATGCATGTTAATTAGTCTAATCA
    CAGAGCGTTCGGGATACGCCATTAGTGGTCTATAGTAGTCAACTGCCGGGATAAATCACGATCCA
    CATTTCATAGGTGTTTCCACCATGTCAACATCGAACTAAAAGGAAAAATATGTGAATGGGTAAAA
    ATGATTAAAAATATTTGTAAAAAATTATTTGAATTTATTTAAAACAATATGCAAGTTGTTTATAG
    GTTGAGTATATTTCAATGGTTTTTGAAAAATCTATGTAAATAAAAAAAATACAATTATTTATATA
    AAATAAAATAATCTTTTTTTTATTATTATGACATTGATGAGAGTATCTAATAATTTGACCCATAA
    CTAATTTGGATAAAAAAAAATCTGATTGACCACTTTTAATTTAATGTATCACTAAACTAAATACC
    CTTTTTTAAAATAGTCTAAACATGAATTAAATATTCAAAAGAAATATTTTACTTGAGATTATTAC
    CTAATATTAATGATAATTTCATTCAACTCCAATAAAATTAATTTTCATGTAAGATATATCTAAAA
    GAAAAGATATATATAAATTTTATTTTCACTAGTAAAAAAAAGTTGATCTAGTTAGTGAAAAACCA
    ACTCATATCCTATAAGAATATGAATTTGATTTTTTTTGTTAAGGTGAGAATTTTATTGATCAATA
    ATTTATAAATATCTATATAAATAATCTTTAGCCTTATGAGTCCTTAGGTCAATTCAACTCACCTA
    AATTTTTTATTATGAAAAAAAAAATTGTATCTTCACAAGATAAATGTGTTGGATTCAATCACTCC
    TTATTAGCTTAATTAGATTATAATTGTAGTCCCCTATATATATATATGTATCATCTTGTCAAATA
    ATAATGAAATATAGAATTTATTTAGACTTAGAGAATAAAATTAAAAACTGTCTGCCATGAAAAAA
    GACGAAGTTAAGAAAAGGGCCAATCATAGAAGATTTTTATGGGCACTTCACGGACACTAACTCAC
    TGTCACAATCATCACTGGGGTTGACAAAAGGACAATATGAAACACTTTTGAGAAGCATGTACCAC
    TCATCCATTTATCAGTGGCTCCCAATTCCCAGAGGCCAGAACTATATATGAAAGAATTGTTGAAC
    GCACGGGCATGAACCCATTCTTGAAGCATCATTGTGTGAGAATATCTTGACCTTGTAAGATGCAA
    CACCTTTTTAAGCCTTAAATTTAAAAAAGGAAAAAAGAAAAATCTTGTCTCTACTTTCTTTTAGC
    ACAAGTGTATAGAAATTCTTAAATATATACACTCTCCTTTATATTGTAGTATCAGTGGCGCAAAT
    CATTATATTTCATTTTTAATAATAAAATTAAGAGCATTAATTTTATAGTTAAAATTGAAAATAAA
    GATAATTTACAGAACTCATTTGACTTAAACTGACAAAATATATATATATATATATATATATATAT
    ATATATATATTGTGAGATGAACATGTTACTTTTTTAACATGCAAAAAGGAGAATATATTTTACAT
    GCATGCACCCATGATAACTTCTATGTATATATCCATACAATACATCGTTCGTATATCGTCTCGTT
    TGTCTTTATTCTCCTCTCAAAATACGACAATAGCAATTTACATTTTTTTTTATAAGCAAATAGTA
    ATTTACATTTAACTTAGTAATGTAGGGATCGAACATAACCACTTGCGATGAATAATTTGCTTTAA
    ATTTTTGTTGATGCGTACCCTTTAACTGTCACTCATGGAATACGATTCTTCAATATCTAGTGCTT
    GATCGTTGACAGAACAACTTAAAACAGAGAATTGATGTTTTGGCTTTTCCATGGATAATAATTAT
    CCCAGTGACATACCAAAGCATAGTAGCTAAGAAGACTTTCACGTAAAAAAAAGTTTCTTTTATTC
    CCTTTTTTAATTTGGTGAATCACAAAAAACCACTTTTGTTTGGTTCCAAAGTTAGGCTCATGGAA
    AGTTTAAACCTCCATAGAATGGTCACGAAGAGATTGCATCTTTGTCTTCACAAAAGCTAACTCCA
    CGTTGAGTAGACTTAACAGCCAGTGGCGAATAGCAAGGATATTTCATTAATTATACGCCACCGGC
    CAAATGTTAACCAATCGTATTATAATTAAGTTCCATCATCATCAAACAATTTAGTAAAGTGCATG
    ACCCAAATTTCTACGATACATATTTATTTATTAAAAATGTAAGAATATTTCAGTCATATTTAAAA
    ATATATATATCAAGAATAATTAACTTTGTACACACGCACTGAATAAAAGATTTGTGACAGACAAG
    GCTTGCATAAAAATTTCTCCTCTAAACTAATTGCTTGTAGGACCTCTCCCACCACTATAGAATCA
    ATATAATTAATCCGCATTAGAAAGTTATATTGTATACAATTTTCTTGAAACATAATTATACTTCA
    TGTTTCACAGACTTATAGTGGATCTTGTGTGGCTAGCTACTGATGAATATTGTTTTTTTTTTTTC
    CTAAGCATCCACTTTGAACAACTTTTCCCATTTCATACAAACAGAATTAATTAGTATTGCGTGCC
    ACCATATGGTACAGTGTTGTACATGCATATAAGCTATTTAATTTAATAATATACAAACATAACGG
    TGTATATAAATAGAGGCAGCATGTGGTGTGTGGTGTAAAAATAAGGACGCAGGCAAATGTATGCA
  TTTGGCATAAGTATATAAGAGAGAGGGAGTAGTACTACTGCAAAGCAAAATCAGAGAGACATGAG
    AAGCTGTGTGTGCTACACGCTATTATTGTTTATTTTCTTCATATGGCTGCGCGTGGCAACGTGCT
    CTTCGTTCACTGACATGGAATCGCTTCTGAAGCTGAAGGACTCCATGAAAGGAGATAAAGCCAAA
    GACGACGCTCTCCATGACTGGAAGTTTTTCCCCTCGCTTTCTGCACACTGTTTCTTTTCAGGCGT
    AAAATGCGACCGAGAACTTCGAGTCGTTGCTATCAACGTCTCGTTTGTTCCTCTCTTCGGTCACC
    TTCCGCCGGAGATCGGACAATTGGACAAACTCGAGAACCTCACCGTCTCGCAGAACAACCTCACC
    GGCGTACTTCCCAAGGAGCTCGCCGCCCTCACTTCCCTCAAGCACCTCAACATCTCTCACAACGT
    CTTCTCCGGCCATTTCCCCGGCCAAATTATCCTTCCGATGACGAAACTGGAGGTCCTCGACGTCT
    ACGACAACAACTTCACCGGACCGCTTCCCGTAGAGTTGGTGAAACTGGAGAAATTAAAATACCTG
    AAGCTCGACGGAAACTATTTCTCCGGCAGCATACCGGAGAGTTACTCGGAGTTTAAGAGCTTGGA
    GTTTTTAAGCTTAAGCACCAATAGCTTATCGGGGAAGATTCCCAAGAGTTTGTCGAAGTTGAAGA
    CGCTGAGGTACCTAAAACTCGGATACAACAACGCTTACGAAGGTGGAATTCCACCGGAGTTTGGC
    AGCATGAAATCTCTGAGATACCTTGACCTCTCTAGCTGCAACCTCAGCGGCGAGATTCCACCGAG
    CCTTGCAAATCTGACAAACCTTGACACGTTGTTCCTGCAAATTAACAACCTCACCGGAACCATTC
    CGTCGGAGCTCTCCGCTATGGTGAGCCTCATGTCACTTGATCTCTCCATCAACGACCTCACCGGT
    GAGATACCGATGAGCTTCTCACAGCTTAGAAACCTCACTCTCATGAACTTCTTCCAAAACAATCT
    TCGCGGCTCAGTTCCGTCCTTCGTCGGCGAGCTTCCGAATCTGGAAACGCTGCAGCTCTGGGATA
    ACAACTTCTCCTTCGTGCTACCTCCGAACCTTGGGCAAAACGGCAAGTTAAAGTTCTTCGACGTC
    ATCAAGAATCACTTCACCGGGTTGATCCCTCGAGATTTGTGTAAGAGTGGGAGGTTACAAACGAT
    CATGATCACAGATAACTTCTTCCGCGGTCCAATCCCTAACGAGATTGGTAACTGCAAGTCTCTCA
    CCAAGATCCGAGCCTCCAATAACTACCTTAACGGCGTGGTTCCGTCAGGGATTTTCAAACTACCT
    TCTGTCACGATAATCGAGCTGGCCAATAACCGTTTTAACGGCGAACTGCCTCCTGAGATTTCCGG
    CGAATCCCTGGGGATTCTCACTCTTTCCAACAACTTATTCAGTGGGAAAATTCCCCCAGCGTTGA
    AGAACTTGAGGGCACTGCAGACTCTCTCACTTGACGCAAACGAGTTCGTTGGAGAAATACCGGGA
    GAGGTTTTTGACCTACCGATGCTGACTGTGGTCAACATAAGCGGCAACAATCTAACCGGACCAAT
    CCCAACGACGTTGACTCGCTGCGTTTCACTCACCGCCGTGGACCTCAGCCGGAACATGCTTGAAG
    GGAAGATTCCGAAGGGAATCAAAAACCTCACGGACTTGAGCATTTTCAATGTGTCGATAAACCAA
    ATTTCAGGGCCAGTCCCTGAGGAGATTCGCTTCATGTTGAGTCTCACCACATTGGATCTATCCAA
    CAACAATTTCATCGGCAAGGTCCCAACCGGGGGTCAGTTCGCGGTCTTCAGCGAGAAATCCTTTG
    CAGGGAACCCCAACCTCTGTACCTCCCACTCTTGCCCGAATTCCTCGTTGTACCCTGACGACGCC
    TTGAAGAAGAGGCGCGGCCCTTGGAGTTTGAAATCCACGAGGGTGATAGTCATCGTGATTGCACT
    GGGCACAGCCGCGCTGCTGGTGGCGGTGACGGTGTACATGATGAGGAGGAGGAAGATGAACCTTG
    CGAAGACGTGGAAGCTGACGGCGTTCCAGCGGCTGAACTTCAAAGCCGAGGACGTGGTGGAGTGT
    CTGAAGGAGGAGAACATAATAGGAAAAGGAGGGGCAGGGATCGTGTACCGCGGGTCCATGCCAAA
    CGGAACAGACGTGGCGATAAAGCGGTTGGTTGGGGCGGGGAGTGGAAGGAACGATTACGGATTCA
    AAGCGGAGATAGAAACGCTGGGGAAGATAAGGCACAGGAACATAATGAGGCTTTTAGGTTACGTG
    TCGAACAAGGAGACGAACTTGCTGCTGTATGAGTACATGCCAAATGGGAGCTTAGGGGAATGGCT
    GCATGGTGCCAAAGGAGGGCACTTGAAGTGGGAAATGAGGTACAAGATTGCGGTGGAAGCTGCTA
    AGGGACTGTGCTATTTGCACCATGATTGTTCCCCTCTTATCATTCACAGGGATGTCAAGTCTAAT
    AATATATTGCTGGATGGGGACTTGGAGGCCCATGTTGCTGATTTTGGCCTTGCCAAGTTCTTGTA
    CGACCCTGGCGCCTCTCAGTCCATGTCCTCCATTGCTGGCTCCTACGGCTACATTGCTCCAGGTT
    CCATTCATTATTATTTTCTCTTTTCCTTCTTCATAATCTTAATATACCATGCAGATAACGTACAA
    CATGCATACTTATACATATAATTTTATCCTTTCAACATATAATCAAATATTTCATATCTAATAAT
    ACCAACTTCATATTATAAACATCACCTAATATAATCAACATGACTTGATAAATAAGACATATAAG
    TTCAATATTTAAACTCATGTGTCTGAAAAAACATTAATTGGAAAAGTCACTCTTAAAAATATTTG
    ATAATATATCAATATGACCATATGATTCCAATTACGATCACAAACTCTGTTAAAAATTCTTGCTG
    AAGATATTAGTCCTTGAATACTAATATAAGAATATCTTGGGTTAGAAAAGTTACTATTTTACTGT
    TAATTCCCGTTTACTTTAGATGGGTTGGAAGTTGAAAAGTTGAGTGATTTAATTTGTTTCTGGTG
    GTTGCGCAGAGTATGCATACACTTTGAAAGTGGACGAGAAAAGTGATGTGTACAGCTTTGGCGTT
    GTGCTGCTGGAGCTGATAATAGGGAGGAAGCCAGTGGGAGAGTTTGGAGACGGGGTGGACATCGT
    TGGATGGGTCAACAAAACGAGATTGGAGCTCGCTCAGCCGTCGGATGCAGCGTTGGTGTTGGCAG
    TGGTGGACCCAAGGTTGAGTGGGTATCCATTGACAAGTGTCATTTACATGTTCAACATAGCTATG
    ATGTGTGTTAAAGAAATGGGGCCCGCTAGGCCTACCATGAGGGAAGTCGTTCATATGCTCTCAGA
  GCCTCCTCACTCTGCTACTCACACTCACAACCTAATTAATCTCTAGTTAATTAAGTTATTTGCTC
    ATCGATCCAGAATCACTTCTTTTCAAAATAAATTAACACAGACGAAAACTGTAGGAATAACTTTC
    ATCTGTTGTTTGTCGGAAGTGAAACAACGAATCAAATGTGAACTATGTATCAAATGTAAGATAGG
    TTTTAATTAATTTTGTAATATTGGTGTCAACTGTCAAGTAATTCGAAGGATTTTCCCCATTGTGC
    ATGTATCAAGA
 
  GmNARK:ATGAGAAGCTGTGTGTGCTACACGCTATTATTGTTTATTTTCTTCATATGGCTGCGCGTGGCAAC
  Glyma12g04390   GTGCTCTTCGTTCACTGACATGGAATCGCTTCTGAAGCTGAAGGACTCCATGAAAGGAGATAAAG
  cDNA (SEQ ID   CCAAAGACGACGCTCTCCATGACTGGAAGTTTTTCCCCTCGCTTTCTGCACACTGTTTCTTTTCA
  NO: 39) Soybean   GGCGTAAAATGCGACCGAGAACTTCGAGTCGTTGCTATCAACGTCTCGTTTGTTCCTCTCTTCGG
  CLV1-like gene   TCACCTTCCGCCGGAGATCGGACAATTGGACAAACTCGAGAACCTCACCGTCTCGCAGAACAACC
    TCACCGGCGTACTTCCCAAGGAGCTCGCCGCCCTCACTTCCCTCAAGCACCTCAACATCTCTCAC
    AACGTCTTCTCCGGCCATTTCCCCGGCCAAATTATCCTTCCGATGACGAAACTGGAGGTCCTCGA
    CGTCTACGACAACAACTTCACCGGACCGCTTCCCGTAGAGTTGGTGAAACTGGAGAAATTAAAAT
    ACCTGAAGCTCGACGGAAACTATTTCTCCGGCAGCATACCGGAGAGTTACTCGGAGTTTAAGAGC
    TTGGAGTTTTTAAGCTTAAGCACCAATAGCTTATCGGGGAAGATTCCCAAGAGTTTGTCGAAGTT
    GAAGACGCTGAGGTACCTAAAACTCGGATACAACAACGCTTACGAAGGTGGAATTCCACCGGAGT
    TTGGCAGCATGAAATCTCTGAGATACCTTGACCTCTCTAGCTGCAACCTCAGCGGCGAGATTCCA
    CCGAGCCTTGCAAATCTGACAAACCTTGACACGTTGTTCCTGCAAATTAACAACCTCACCGGAAC
    CATTCCGTCGGAGCTCTCCGCTATGGTGAGCCTCATGTCACTTGATCTCTCCATCAACGACCTCA
    CCGGTGAGATACCGATGAGCTTCTCACAGCTTAGAAACCTCACTCTCATGAACTTCTTCCAAAAC
    AATCTTCGCGGCTCAGTTCCGTCCTTCGTCGGCGAGCTTCCGAATCTGGAAACGCTGCAGCTCTG
    GGATAACAACTTCTCCTTCGTGCTACCTCCGAACCTTGGGCAAAACGGCAAGTTAAAGTTCTTCG
    ACGTCATCAAGAATCACTTCACCGGGTTGATCCCTCGAGATTTGTGTAAGAGTGGGAGGTTACAA
    ACGATCATGATCACAGATAACTTCTTCCGCGGTCCAATCCCTAACGAGATTGGTAACTGCAAGTC
    TCTCACCAAGATCCGAGCCTCCAATAACTACCTTAACGGCGTGGTTCCGTCAGGGATTTTCAAAC
    TACCTTCTGTCACGATAATCGAGCTGGCCAATAACCGTTTTAACGGCGAACTGCCTCCTGAGATT
    TCCGGCGAATCCCTGGGGATTCTCACTCTTTCCAACAACTTATTCAGTGGGAAAATTCCCCCAGC
    GTTGAAGAACTTGAGGGCACTGCAGACTCTCTCACTTGACGCAAACGAGTTCGTTGGAGAAATAC
    CGGGAGAGGTTTTTGACCTACCGATGCTGACTGTGGTCAACATAAGCGGCAACAATCTAACCGGA
    CCAATCCCAACGACGTTGACTCGCTGCGTTTCACTCACCGCCGTGGACCTCAGCCGGAACATGCT
    TGAAGGGAAGATTCCGAAGGGAATCAAAAACCTCACGGACTTGAGCATTTTCAATGTGTCGATAA
    ACCAAATTTCAGGGCCAGTCCCTGAGGAGATTCGCTTCATGTTGAGTCTCACCACATTGGATCTA
    TCCAACAACAATTTCATCGGCAAGGTCCCAACCGGGGGTCAGTTCGCGGTCTTCAGCGAGAAATC
    CTTTGCAGGGAACCCCAACCTCTGTACCTCCCACTCTTGCCCGAATTCCTCGTTGTACCCTGACG
    ACGCCTTGAAGAAGAGGCGCGGCCCTTGGAGTTTGAAATCCACGAGGGTGATAGTCATCGTGATT
    GCACTGGGCACAGCCGCGCTGCTGGTGGCGGTGACGGTGTACATGATGAGGAGGAGGAAGATGAA
    CCTTGCGAAGACGTGGAAGCTGACGGCGTTCCAGCGGCTGAACTTCAAAGCCGAGGACGTGGTGG
    AGTGTCTGAAGGAGGAGAACATAATAGGAAAAGGAGGGGCAGGGATCGTGTACCGCGGGTCCATG
    CCAAACGGAACAGACGTGGCGATAAAGCGGTTGGTTGGGGCGGGGAGTGGAAGGAACGATTACGG
    ATTCAAAGCGGAGATAGAAACGCTGGGGAAGATAAGGCACAGGAACATAATGAGGCTTTTAGGTT
    ACGTGTCGAACAAGGAGACGAACTTGCTGCTGTATGAGTACATGCCAAATGGGAGCTTAGGGGAA
    TGGCTGCATGGTGCCAAAGGAGGGCACTTGAAGTGGGAAATGAGGTACAAGATTGCGGTGGAAGC
    TGCTAAGGGACTGTGCTATTTGCACCATGATTGTTCCCCTCTTATCATTCACAGGGATGTCAAGT
    CTAATAATATATTGCTGGATGGGGACTTGGAGGCCCATGTTGCTGATTTTGGCCTTGCCAAGTTC
    TTGTACGACCCTGGCGCCTCTCAGTCCATGTCCTCCATTGCTGGCTCCTACGGCTACATTGCTCC
    AGAGTATGCATACACTTTGAAAGTGGACGAGAAAAGTGATGTGTACAGCTTTGGCGTTGTGCTGC
    TGGAGCTGATAATAGGGAGGAAGCCAGTGGGAGAGTTTGGAGACGGGGTGGACATCGTTGGATGG
    GTCAACAAAACGAGATTGGAGCTCGCTCAGCCGTCGGATGCAGCGTTGGTGTTGGCAGTGGTGGA
    CCCAAGGTTGAGTGGGTATCCATTGACAAGTGTCATTTACATGTTCAACATAGCTATGATGTGTG
    TTAAAGAAATGGGGCCCGCTAGGCCTACCATGAGGGAAGTCGTTCATATGCTCTCAGAGCCTCCT
  CACTCTGCTACTCACACTCACAACCTAATTAATCTCTAG
 
  GmNARK:   MRSCVCYTLLLFIFFIWLRVATCSSFTDMESLLKLKDSMKGDKAKDDALHDWKFFPSLSAHCFFS
  Glyma12g04390   GVKCDRELRVVAINVSFVPLFGHLPPEIGQLDKLENLTVSQNNLTGVLPKELAALTSLKHLNISH
  protein (SEQ   NVFSGHFPGQIILPMTKLEVLDVYDNNFTGPLPVELVKLEKLKYLKLDGNYFSGSIPESYSEFKS
  ID   LEFLSLSTNSLSGKIPKSLSKLKTLRYLKLGYNNAYEGGIPPEFGSMKSLRYLDLSSCNLSGEIP
  NO: 40) Soybean   PSLANLTNLDTLFLQINNLTGTIPSELSAMVSLMSLDLSINDLTGEIPMSFSQLRNLTLMNFFQN
  CLV1-like gene   NLRGSVPSFVGELPNLETLQLWDNNFSFVLPPNLGQNGKLKFFDVIKNHFTGLIPRDLCKSGRLQ
    TIMITDNFFRGPIPNEIGNCKSLTKIRASNNYLNGVVPSGIFKLPSVTIIELANNRFNGELPPEI
    SGESLGILTLSNNLFSGKIPPALKNLRALQTLSLDANEFVGEIPGEVFDLPMLTVVNISGNNLTG
    PIPTTLTRCVSLTAVDLSRNMLEGKIPKGIKNLTDLSIFNVSINQISGPVPEEIRFMLSLTTLDL
    SNNNFIGKVPTGGQFAVFSEKSFAGNPNLCTSHSCPNSSLYPDDALKKRRGPWSLKSTRVIVIVI
    ALGTAALLVAVTVYMMRRRKMNLAKTWKLTAFQRLNFKAEDVVECLKEENIIGKGGAGIVYRGSM
    PNGTDVAIKRLVGAGSGRNDYGFKAEIETLGKIRHRNIMRLLGYVSNKETNLLLYEYMPNGSLGE
    WLHGAKGGHLKWEMRYKIAVEAAKGLCYLHHDCSPLIIHRDVKSNNILLDGDLEAHVADFGLAKF
    LYDPGASQSMSSIAGSYGYIAPEYAYTLKVDEKSDVYSFGVVLLELIIGRKPVGEFGDGVDIVGW
    VNKTRLELAQPSDAALVLAVVDPRLSGYPLTSVIYMFNIAMMCVKEMGPARPTMREVVHMLSEPP
    HSATHTHNLINL
 
  GmCLV1A:   AGCTTCGCATAAGTAACGTGAGTTTAGTTAAGTCGAGCTAGTCGCCTTTTTCTATGGTTGGTTAT
  Glyma11g12190   GTGCAGTAGTGAATGTTGTGTAGTATCTTGCGAGGCCATGTTTGGTGTGACAAGCCCGAAAGTGA
  gDNA + about   CTTGAGGGGAACAAAATAGCTTTTGTCCAAACATGCTAACTTGTCATCATGACATCTACTTCTCT
  1.6 kb promoter   GGTCATGGCAGCTCTGATTAATAATTTAAGTGATCATAATATTAGAAGTTAAAAAATTATAACAT
  and 5′UT   CTTTAATTATTTTTATTATTTTATATAATCTTAAAAATTATTTCAAACTTCTTTAAACAATGTTG
  sequence (SEQ   AATAAGATCATGTATTTTTTTTTTTTCCTTACGTAGTAGTATCCTGGCAGTCACCCAGGAGCAAA
  ID   TGATGTAGATAAATCCTTTTTACTAAAATAGTCTTGGAGCAATATTTAAGAGGGGACCATTTTAT
  NO: 41) Soybean   GATCTTTTCTATCTTAATAGTGGCGTTAGAATAACACTTTTTTAAGCTTTAAATAAAAAATAAAA
  CLV1-like gene   AAATATTATCTTTACTTTCTTTTAGCAATTATTCCTACGTGTAGAGAAACTGTTAAATACACTCT
    CCTTTGTATTGTATAATGTTGCATTGTATCAGTTGTCCAAATTAATCACAGTATATTAGTAATAA
    AATTATGAACATTAATTTTATTCTTAAAATTTAGTTAAATATTGATAATTCACATAACTCGTGAC
    TTAATCTAATTATATATAGAAGATCATGTTAGTATGTTACCTTTTTAAAATGCAAAATGAAGAAT
    CTGTTACATGCACCCACTGGGTAAGTTGATAACTATTATGTATCTATATATCGTCTGGATATTGT
    CTGTTTCATTCTCTTCTCAAAAGAACAAAATGGTAATTTACATTTAACTTAGAAATGTTTGGGAC
    AGAATCACTAGCTTGCAGATGAATAATTTGTTTTAAATTCTTATTGATGCATAATACCCTCTACT
    TGTCACTCATAGAATACGATTCTGCAATAACTAGTGCTTGATCCTTGACAGAACAAATTAAAACA
    GAGAATTGATGCATTGGCTTTTCCATGGACAATAATTATCCCATTGATGTACTAAAGCACAGTAA
    CTAGGTAGGAAGACCTCCACCTAAAGAAACTTTCTTTTATTCTCCTTTAATTTTAAATTTGGTGA
    ATCACTTAAAACAACTTTTGTTTCATTCCAAAGTTAGGCTCATGGAAAGCTTAAACCTAGTTAAA
    TAGCCACGAAAGAGATTGCATCTTTGTTTTCACAAAAGCTAACTGCGCGTTTGTGAAGCTAGTGA
    TGCATAGTATATATATATTTTTTTCTCGGCATCCACTTTGAGAACTACTTTTTTTTTCATTTTCA
    TAGAAACAGAATTGAAGTAGTATAACATGCCACCATGAACAGTACAGTGATGTACATGAATAAAT
    GCATGCTATTCAATATAATGTATAATATAACGGTGTATATATAAATAGAGACTGCATGAGGTGTG
    TGGTGTCAACATATAATAAGGACGCAGCGTAGGTATAATAGTGAGTACCGCGAAGAAAGATAAGA
    GCCAGAGCCATGAGAAGCTGTGTGCTTTACACGCTATTATTGTTTGTTTTCTGCATATGGGTTCC
  CATGGCAACGTGCTCTTCGTTCAGTGACATGGATGCGTTACTAAAGCTGAAGGAGTCCATGAAAG
    GAGACGAAGCCAAAGACGACGCACTCCATGACTGGAAGTTTTCCACATCGCATTCTGCACACTGT
    TTCTTTTCAGGCGTAACATGTGACCAAGACCTTCGAGTCGTTGCTATCAACGTCTCCTTTGTTCC
    TCTCTTCGGTCACATTCCGCCGGAGATCGGAAACTTGGACAAGCTGGAAAATCTCACAATCGTGA
    ACAACAATCTAACCGGTGTACTCCCCATGGAGCTTGCCGCCCTCACTTCCCTCAAGCACCTCAAC
    ATATCTCACAACCTCTTCACCGGCGATTTCCCCGGCCAAGCCACTCTTCCGATGACGGAACTTCA
    AGTCCTCGACGTCTACGACAACAACTTCACCGGACCGCTTCCGGAAGAATTCGTGAAACTGGAGA
    AACTAAAATACCTGAAACTCGACGGAAACTATTTTACCGGCAGCATACCGGAGAGTTACTCGGAG
    TTTAAGAGCTTGGAGTTTTTGAGCTTAAACACCAACAGCTTATCGGGGAGGATTCCGAAGAGTTT
    GTCCAAGTTGAAGACTCTGAGGATTCTCAAACTCGGATACAGCAACGCTTACGAAGGTGGAATTC
    CTCCGGAGTTCGGCACCATGGAATCTCTGAGATTCCTCGACCTCTCAAGCTGCAACCTCAGCGGC
    GAGATTCCACCGAGTCTTGCAAATCTGACAAACCTAGACACGTTGTTCTTGCAAATGAACTTCCT
    CACCGGAAGCATTCCGTCTGAACTCTCTTCTTTGGTGAGGCTCATGGCACTGGATCTCTCCTGCA
    ACAGCCTCACCGGGGAGATTCCAGAGAGCTTTTCTCAGCTGAGAAACCTCACTCTCATGAACTTG
    TTCCGCAACAATCTTCACGGCCCTATTCCGTCCTTGCTGAGCGAGCTTCCCAATCTGAATACGCT
    GCAGCTCTGGGAGAATAACTTCTCCTCTGAGCTCCCGCAGAACCTGGGGCAAAACGGGAGGCTGA
    AGTTCTTCGACGTCACGAAGAATCACTTCAGCGGGTTGATCCCTCGGGATTTGTGCAAGAGTGGG
    AGGTTACAAATCTTCATTATCACAGATAACTTCTTTCATGGCCCAATCCCTAACGAGATTGCTAA
    CTGCAAGTCTCTAACCAAGATCCGAGCCTCCAATAACTACCTTAACGGCGCAGTTCCGTCGGGGA
    TTTTCAAGCTACCTTCCGTCACGATAATCGAGTTGGCCAATAACCGTTTTAACGGAGAACTGCCT
    CCCGAAATTTCCGGCGATTCACTCGGGATTCTCACTCTTTCCAACAACTTATTCACTGGGAAAAT
    TCCCCCAGCGTTGAAGAACTTAAGGGCACTGCAGACTCTGTCACTTGACACGAACGAGTTCCTTG
    GAGAAATCCCGGGGGAGGTTTTTGACCTACCAATGCTGACTGTGGTCAACATAAGCGGCAACAAT
    CTCACCGGACCAATCCCAACGACGTTTACTCGCTGCGTTTCACTCGCCGCCGTTGATCTCAGCCG
    GAACATGCTAGTTGAGGATATTCCTAAGGGGATTAAGAACCTCACGGTCTTGAGCTTTTTCAATG
    TCTCGAGAAACCATTTAACAGGGCCAGTCCCTGACGAGATAAAATTCATGACGAGCCTCACCACG
    CTGGATCTCTCCTACAACAATTTCACAGGCAAGGTCCCCAACGAGGGTCAGTTTTTGGTCTTCAA
    CGACAACTCGTTTGCAGGGAACCCTAACCTCTGTTCCATTCACGGATGCACTTTAAGCATTGTGG
    GGGCAGCTGCCCCTATCAACATTTTAACATTTGTAAATATAGTATGTACAATTATAGTAATTTAT
  AAATTGCTTGTATAA
 
  GmCLV1A:ATGGCAACGTGCTCTTCGTTCAGTGACATGGATGCGTTACTAAAGCTGAAGGAGTCCATGAAAGG
  Glyma11g12190   AGACGAAGCCAAAGACGACGCACTCCATGACTGGAAGTTTTCCACATCGCATTCTGCACACTGTT
  cDNA (SEQ ID   TCTTTTCAGGCGTAACATGTGACCAAGACCTTCGAGTCGTTGCTATCAACGTCTCCTTTGTTCCT
  NO: 42) Soybean   CTCTTCGGTCACATTCCGCCGGAGATCGGAAACTTGGACAAGCTGGAAAATCTCACAATCGTGAA
  CLV1-like gene   CAACAATCTAACCGGTGTACTCCCCATGGAGCTTGCCGCCCTCACTTCCCTCAAGCACCTCAACA
    TATCTCACAACCTCTTCACCGGCGATTTCCCCGGCCAAGCCACTCTTCCGATGACGGAACTTCAA
    GTCCTCGACGTCTACGACAACAACTTCACCGGACCGCTTCCGGAAGAATTCGTGAAACTGGAGAA
    ACTAAAATACCTGAAACTCGACGGAAACTATTTTACCGGCAGCATACCGGAGAGTTACTCGGAGT
    TTAAGAGCTTGGAGTTTTTGAGCTTAAACACCAACAGCTTATCGGGGAGGATTCCGAAGAGTTTG
    TCCAAGTTGAAGACTCTGAGGATTCTCAAACTCGGATACAGCAACGCTTACGAAGGTGGAATTCC
    TCCGGAGTTCGGCACCATGGAATCTCTGAGATTCCTCGACCTCTCAAGCTGCAACCTCAGCGGCG
    AGATTCCACCGAGTCTTGCAAATCTGACAAACCTAGACACGTTGTTCTTGCAAATGAACTTCCTC
    ACCGGAAGCATTCCGTCTGAACTCTCTTCTTTGGTGAGGCTCATGGCACTGGATCTCTCCTGCAA
    CAGCCTCACCGGGGAGATTCCAGAGAGCTTTTCTCAGCTGAGAAACCTCACTCTCATGAACTTGT
    TCCGCAACAATCTTCACGGCCCTATTCCGTCCTTGCTGAGCGAGCTTCCCAATCTGAATACGCTG
    CAGCTCTGGGAGAATAACTTCTCCTCTGAGCTCCCGCAGAACCTGGGGCAAAACGGGAGGCTGAA
    GTTCTTCGACGTCACGAAGAATCACTTCAGCGGGTTGATCCCTCGGGATTTGTGCAAGAGTGGGA
    GGTTACAAATCTTCATTATCACAGATAACTTCTTTCATGGCCCAATCCCTAACGAGATTGCTAAC
    TGCAAGTCTCTAACCAAGATCCGAGCCTCCAATAACTACCTTAACGGCGCAGTTCCGTCGGGGAT
    TTTCAAGCTACCTTCCGTCACGATAATCGAGTTGGCCAATAACCGTTTTAACGGAGAACTGCCTC
    CCGAAATTTCCGGCGATTCACTCGGGATTCTCACTCTTTCCAACAACTTATTCACTGGGAAAATT
    CCCCCAGCGTTGAAGAACTTAAGGGCACTGCAGACTCTGTCACTTGACACGAACGAGTTCCTTGG
    AGAAATCCCGGGGGAGGTTTTTGACCTACCAATGCTGACTGTGGTCAACATAAGCGGCAACAATC
    TCACCGGACCAATCCCAACGACGTTTACTCGCTGCGTTTCACTCGCCGCCGTTGATCTCAGCCGG
    AACATGCTAGTTGAGGATATTCCTAAGGGGATTAAGAACCTCACGGTCTTGAGCTTTTTCAATGT
    CTCGAGAAACCATTTAACAGGGCCAGTCCCTGACGAGATAAAATTCATGACGAGCCTCACCACGC
    TGGATCTCTCCTACAACAATTTCACAGGCAAGGTCCCCAACGAGGGTCAGTTTTTGGTCTTCAAC
    GACAACTCGTTTGCAGGGAACCCTAACCTCTGTTCCATTCACGGATGCACTTTAAGCATTGTGGG
    GGCAGCTGCCCCTATCAACATTTTAACATTTGTAAATATAGTATGTACAATTATAGTAATTTATA
  AATTGCTTGTATAA
 
  GmCLV1A:   MATCSSFSDMDALLKLKESMKGDEAKDDALHDWKFSTSHSAHCFFSGVTCDQDLRVVAINVSFVP
  Glyma11g12190   LFGHIPPEIGNLDKLENLTIVNNNLTGVLPMELAALTSLKHLNISHNLFTGDFPGQATLPMTELQ
  protein (SEQ   VLDVYDNNFTGPLPEEFVKLEKLKYLKLDGNYFTGSIPESYSEFKSLEFLSLNTNSLSGRIPKSL
  ID   SKLKTLRILKLGYSNAYEGGIPPEFGTMESLRFLDLSSCNLSGEIPPSLANLTNLDTLFLQMNFL
  NO: 43) Soybean   TGSIPSELSSLVRLMALDLSCNSLTGEIPESFSQLRNLTLMNLFRNNLHGPIPSLLSELPNLNTL
  CLV1-like gene   QLWENNFSSELPQNLGQNGRLKFFDVTKNHFSGLIPRDLCKSGRLQIFIITDNFFHGPIPNEIAN
    CKSLTKIRASNNYLNGAVPSGIFKLPSVTIIELANNRFNGELPPEISGDSLGILTLSNNLFTGKI
    PPALKNLRALQTLSLDTNEFLGEVPGEVFDLPMLTVVNISGNNLTGPIPTTFTRCVSLAAVDLSR
    NMLVEDIPKGIKNLTVLSFFNVSRNHLTGPVPDEIKFMTSLTTLDLSYNNFTGKVPNEGQFLVFN
    DNSFAGNPNLCSIHGCTLSIVGAAAPINILTFVNIVCTIIVIYKLLV
 
  Glyma18g51820   GCCTGCCCCTTAGTCATGTGCAAAATAGTGCTAAGATCTGTATTGTAAAATGGCCACATTGGTCT
  gDNA + about   TAGTAAAAGAGTTATGCATATGCTGCACTGGTAGCACCCAGCCTGCACTTCGTAATATGATGATT
  3.7 kb promoter   GTGTATTTTTGTTTACTTTTGAGGTGAAGCTGCGATGCATTAGGCTAGGGATTTGTGTATGTTGT
  and 5′UT (SEQ   GTACATTGGTTTTTGTGAAGGTGTTGTTGTGGCTGTAATTTACATTTTTGTATTTTTGGGATTAC
  ID   TTGGTGGGACATGTGCTGAGGATGCCATGTCCCTAGTTCTCTAATGTTCTGATGTATTATTTATT
  NO: 44) Soybean   TATATTGATAAAAAAAATTATATACTTTCAAAGGCAAAAAGATAAAGAAAACTATCAATCACCTG
  CRN-like gene   CTATTTTAGAAATACCCCCCTCCCAAAAGAAAAACCCAAATTATTGTAATCATATAAAGTTTCGG
    TGTTGAAAAGACGGCGTGGGGCACCATGTTGAAGGCTTGAGAATTTTTTGGTCAATTGAATCAAA
    AAGTGAAGTGGTCCATTTGACCCCCAGTTTGCAATGGTAAATTCAAGAATTGGGTGGAAGTGTCC
    ATTGTATTTTTCGTATCCAACAATAAAGAATCACAGTTGTTGCACAGATACAACAATCAAAGGTC
    TAGATATTTTGTAGTCTTATAATAGGAATTTTCACTGTTTTACACAAACATTTTTTTATCTACAA
    AACAAACCGTGAGGAATCTTGTAGGTTATAGTGGCCAACACTCATGTTGCGTTAACACAGCTATC
    AACTAAAACTCAACTTTTGTCACGGGTGACCTCAACATAATTATTGATATTACTGACAGAGTAAC
    AACACCTGAAGTGGGCCCTGTTGAACTGGGTTATGACTAATGACGAGACCACAACTTAGAGGATA
    GATACATTTCTAATCTTTCAAATAAATACAAGTGATATTAACTTGGTCTTTGAAAAATATGAACA
    TCAATTCTGTTTTTTAATTATAAAAACAATAGTAATTTGATTTGATTTACTGAAAAAATTAGCGT
    CAATTTAAAATTTCAGTATTAAAAAATGATACGATTTACCATCTTAAGTATTGCACGCAAAAGAT
    TATTTTAATATCATTTTTCAATTATTAAGAGAAAAAAAGTGACGTTAATATCTTAGGACAAAATT
    AATATCTCTGGCATTTTAAGAAAATAAAGAGAATACTTATAAAATAAGACCACAATTCACGAAAT
    CTTATATTAAATATGGTCCTGATAATTCCAATTTGTATAAACTTATTAAAATAATACTTATAGGG
    AAAAAATAGAGAGGCAAATAAATTAAAATCAAATTATGTATTTTTACTTTTGGAGAATTTAAATA
    AGAGAATTTCTTAAAACTTGAGTTAGATAAGTTGATTTTAATTTGTGGGAGATTCTTTTTTATTA
    TATGTCTTTATTTTTTTCTCAGTATTTTTTTTTTTTGGAAAATTTTACCTAAACTGAAATTAAGC
    ATTGTGGAGAATACTTTCAGGGAAAATGACTCAATGATTTAGCGTGTGATTTAAGCATAAATTTT
    GGTACAAGAGTTTGATTAACTATTAATTAAATTAATTTAGAAAGGTCAAGGTCATTTTCACACAA
    TTCTATTCCCTTGCTCGAGACCACTTTTCAAGTATAAATTTATGACTAATGGGTCAAAACATACA
    ATGCCTTGTGTAAATAGTTATGAACGATATTAATATTTTTATGAAAATGATAGTTGCACCAAATA
    TGTGAAATTCGCAATCTGAATTATCTGTTGCATTTGGCTTGGTTTCATTTTGTTAGGTTATTATT
    ATTATTTTTTTTTAAAAAGGAACTGACTGTATCCAATTATATGTCTGTTTTTAAAATTTGAAAGA
    AATAGTTTTAAACCATTTAATATAGCTATAATATATATTTAAGTTAATCTTAGCTATATATTTTG
    TATTAAAATGTATATTTGCTATAATAATTAACTCTAGTAATTTACCAAATGGATATTATTTGTAA
    AGGCTTGATTTGGGTTATACTAGTAATTTAAAATCTACGTACTTACTATTTCTGATTTCAAAATG
    TCTCATGCCACAAATGAACAAAACAATCATGATAATTTATTCATACTATTATTGCTTGCTCATTC
    ACTCACCCCACAGTGCTAGATCCTCGGACTCGAATAAATCATTTATTATGCTTAGATAATTCGAT
    TTATTTTTATTCAATGCAACACTCATTCAATTGCACTACCCTCCTATTCCTATATCACATTAATA
    TGAAGAGTTAATCTTATCCTCTCGATTCATTTTCTTTTTAAATTTAAGGGGTATAATGAGAAATT
    AATTTTGACTATTAAATTTTAAAAACAATCCAAAAATGTCATAAAGAATTTTTCCTATTCCACGA
    GAGAACTTGAAAGTTAAAATTTGATTAAAATCTTATTAAAGGCGTTCCTAATCCTAGCAACTTCC
    ACCTATCACAGAGAAAAAAAAAGGAAAAGAAAAGGTAAGATAGAAAGAAAGAAGGAAAAGTAAAA
    GCATGCAAATATAGAATTATAAATACTAAAAAATATTGTTAAGATATTAGTTAAAAAATTATTAA
    GATACACAAAATTACATTATACACAATTTTTTATAATCTTTAAAATAAATATTTTTTATTTTATT
    AATATCCTAAAGATATTAGTTAATTAACATTCATGTATTATTATTTGAAATTGAAACGTAAGTAG
    TAATTAAAAGCAAATTATTCTATCGAAAAAGAGATAACTTTATTAATGACACACACCAAACATAC
    CAATCGCTAGAGTTGTTAACCACTCACTCATATAGCATATCACAAATTCCCATGCAACCTTAATT
    CAACGGTCCAGATGCAGTCTGATGAGATCAGACGGTCGAGACGAACTGTACATTCTCCCTCTCAC
    GGATTTCGATGTTTCTCTTTCGGACCAAATGTGGGGCCCACATAGTACTGTGTCCTGAGTGCTGG
    CTACTCACAAAGGCGGGAACCAGTTTTTGTCGCAGAAGAGGTATGGCTCTTTGTTTGTTGTCATC
    AGATGAGAGAGAAACAAAACAAAGAGACAATCACTGAATCACTCTCACTCACTCTGCATGCTGTG
    TGCGTGACTCTGTCATTGTGTTTTGTGTTTTAAGCACTTTGCAGTTTAGTTTCTGAGGAGCGTTT
    TTTTTTTTTTCTTTCTTATGAGTGTGTGTCTGTTCTTAGTTGCTGTTATTGTTGTTCAAGTTTCG
    GTTACTACTACTACTACCACATGTCCATGCCCCTTCAATTTCTGTTCAACTTTGTGACTTTTTGT
    TTGGTTTCTAAGGAAAAAGATTGCAACTTGTTTCTGGGTCTAGTTTGCTTTTGGTTGGGTTTGTT
    AGTCACCGCTGGCAACTCGGAATAGTGGGTTTTTTTTTGGAGGGTGTTTTTTTTTTCTTCTTTTG
    GAGGTTCAAATTCTTGTTCTGATTCGTGTGAAGGTGGAAAATTTATGGGTGCTGAGAGGAGGAAA
    AAGATGGGATTTGGTGGAATAAATGTAAAACTATTCGGCGACAACATGTCTGCTTGCTTTTTTGG
    GACGGCTTTCTTGTGAAGATTTTGGGTTTAAAAGGTTGAGGAAGATGCTTATGCCTTATGCTTAT
    GCTTGCAACTTTTTTTTTAAAACCCATTTTAGCATCAAGTATAAAAGTTTCTTCTTGGTCTTGTT
    TCCAAGTGTTTGAGGTGATGGGGGTTTTGAGCATGTGAGTGATTCATGCCTCATTTTGGAGCTTC
  TGAGATTGGTTTCTGGTTGTGGCTTTGTTTGTTTTGTGTTGTGCTTTCATGTTTAGGAAAAGGCA
    CACCCTTTCTTCTCTTGCAAGGGAATTGTTGGCATTTCAGCCACTTTTTCTTCTCTTCTTGTTCA
    GCTTGCACCACAACACTATGCAGTGTCAAGGAAGGTTGAGTAAACATGTTTCTTCTGAGCCTCCC
    TCACCTTCTAGGTCAACACCATCACCACCATCTTCATCAGGATACAAGGATGACCCTAGGAAGAT
    AATTTTGAGCATGGTTTTAGGAGCAGTCACTGGACTAGTTTCTTCTGCTCTCTTTGCACTTGTGG
    TTCGTTGTGTTGTTCAGTATCTGAACCGCACACCAATCCTCAAGGGACCTGTCATATTCTCCCCC
    AAAATTGCCCCCATGACACTCCAATCAGCTTTGGCAAAGGAAAACCACTTGCTCGGTTCGAGTCC
    TAATGGGAAGTACTACAAAACTGTGCTTGACAATGGACTCACTATTGCAGTCAAAAGGCTAACAC
    CCTTTGAGAGTAATTCCCCGGAGGCTAAGAGAAAATCAGTGAAGAGGCAGATACAAACTGAGCTT
    GAGCTTCTTGCAAGCCTTAGGCATAGGAACTTGATGAGTTTAAGGGCCTATGTTCGTGAGCCTGA
    TGGGTTCTCATTGGTTTATGATTATGTTTCCACTGGGAGTCTTGCTGATGTGTTGAGTAAAGTGA
    GGGAGAATGAGTTGCCCTTTGGTTGGGAAGTTAGGCTCAGGATTGCTGTTGGTGTGGTGAAGGGT
    CTTCAGTATCTTCATTTCACTTGTGTGCCTCAGATTCTGCACTACAACTTGAAGCCCACAAATGT
    GATGTTGGATGCTGAGTTTGAACCTAGATTGGCAGATTATGGGTTGGCTAAACTTCTACCCAATT
    TGGATGGAGGAAGTTCTCTCTACACTCCTCCTGAATGTTTCCATAATTGCAGGTAAGACAAATTT
    CAATCATACTCATTCACTAGTGTTTTGAACTTGGTCTGTTTCTGTTCTTTCACTTTTTTACACCA
    ATAGGGTAATTAGGTGGTTGATATTGGGAATTTGTTTGATTCGTTACCTTTTCAAAAGCTCCACA
    CCTCATTGGTTTTTTGCCCCCTTTGTAGTACCCTAATGAAAGACTCTTGTTTTGAAACGAAATTA
    CTATTCTGTAATCTGTATTGTCATTGTATCATTTGCTGATTGAATTTGGTATTATTTAATAAAGA
    CTTTGCTATTTGTTTTTGTAACTACCCATTACTTCCTGATGTCAAGTTTTAGACCTTAGGCAGTT
    GGCACTAAGTCTGGTCCAAATGAATAATATAGTTTATAGTTCACATGCTGCAAACTACTAAACCT
    AGATTGGTGAGTGAGACCACAACTAAATTATAATAATAATTGACAAAGGTTTTTTTTCCTAATTT
    AACTTGGAATACTTCTAGTTTTTCAGTGGTGTATATTTGGATGCATCAATATCAATAGCAATAAG
    TAATAACAATAAAAGATTGCTTGATTGATGGCATTGCATATATGGGTATGGTATTGCCAATAAGA
    TGTTTATTTTAACTTCATTCCATTCTTGTATATGTGGAGCTTCATGGTATTCAGATTGAATGGTG
    TTTTTTGGCAATTTCAGCAGGTACACTGACAAAAGTGACATCTTTAGTTTTGGCATGATACTAGG
    TGTTTTGTTAACTGGTAAGGATCCTACAGATCCATTCTTTGGAGAAGCAGCCAGTGGGGGAAGTT
    TGGGATGTTGGCTGAGACACTTGCAGCAAGCGGGCGAGGCGCACGAAGCTCTAGATAAGAGCATG
    TTAGGGGAAGAAGGTGAGGAAGATGAGATGCTAATGGCGGTTAGGATTGCTGCTGCATGCCTCTC
    TGATATGCCTGCAGATAGGCCTTCTAGTGATGAGCTTGTTCACATGCTAACGCAACTGCACAGTT
  TTTGA
 
  Glyma18g51820ATGTTTAGGAAAAGGCACACCCTTTCTTCTCTTGCAAGGGAATTGTTGGCATTTCAGCCACTTTT
  cDNA (SEQ ID   TCTTCTCTTCTTGTTCAGCTTGCACCACAACACTATGCAGTGTCAAGGAAGGTTGAGTAAACATG
  NO: 45) Soybean   TTTCTTCTGAGCCTCCCTCACCTTCTAGGTCAACACCATCACCACCATCTTCATCAGGATACAAG
  CRN-like gene   GATGACCCTAGGAAGATAATTTTGAGCATGGTTTTAGGAGCAGTCACTGGACTAGTTTCTTCTGC
    TCTCTTTGCACTTGTGGTTCGTTGTGTTGTTCAGTATCTGAACCGCACACCAATCCTCAAGGGAC
    CTGTCATATTCTCCCCCAAAATTGCCCCCATGACACTCCAATCAGCTTTGGCAAAGGAAAACCAC
    TTGCTCGGTTCGAGTCCTAATGGGAAGTACTACAAAACTGTGCTTGACAATGGACTCACTATTGC
    AGTCAAAAGGCTAACACCCTTTGAGAGTAATTCCCCGGAGGCTAAGAGAAAATCAGTGAAGAGGC
    AGATACAAACTGAGCTTGAGCTTCTTGCAAGCCTTAGGCATAGGAACTTGATGAGTTTAAGGGCC
    TATGTTCGTGAGCCTGATGGGTTCTCATTGGTTTATGATTATGTTTCCACTGGGAGTCTTGCTGA
    TGTGTTGAGTAAAGTGAGGGAGAATGAGTTGCCCTTTGGTTGGGAAGTTAGGCTCAGGATTGCTG
    TTGGTGTGGTGAAGGGTCTTCAGTATCTTCATTTCACTTGTGTGCCTCAGATTCTGCACTACAAC
    TTGAAGCCCACAAATGTGATGTTGGATGCTGAGTTTGAACCTAGATTGGCAGATTATGGGTTGGC
    TAAACTTCTACCCAATTTGGATGGAGGAAGTTCTCTCTACACTCCTCCTGAATGTTTCCATAATT
    GCAGCAGGTACACTGACAAAAGTGACATCTTTAGTTTTGGCATGATACTAGGTGTTTTGTTAACT
    GGTAAGGATCCTACAGATCCATTCTTTGGAGAAGCAGCCAGTGGGGGAAGTTTGGGATGTTGGCT
    GAGACACTTGCAGCAAGCGGGCGAGGCGCACGAAGCTCTAGATAAGAGCATGTTAGGGGAAGAAG
    GTGAGGAAGATGAGATGCTAATGGCGGTTAGGATTGCTGCTGCATGCCTCTCTGATATGCCTGCA
  GATAGGCCTTCTAGTGATGAGCTTGTTCACATGCTAACGCAACTGCACAGTTTTTGA
 
  Glyma18g51820   MFRKRHTLSSLARELLAFQPLFLLFLFSLHHNTMQCQGRLSKHVSSEPPSPSRSTPSPPSSSGYK
  protein (SEQ   DDPRKIILSMVLGAVTGLVSSALFALVVRCVVQYLNRTPILKGPVIFSPKIAPMTLQSALAKENH
  ID   LLGSSPNGKYYKTVLDNGLTIAVKRLTPFESNSPEAKRKSVKRQIQTELELLASLRHRNLMSLRA
  NO: 46) Soybean   YVREPDGFSLVYDYVSTGSLADVLSKVRENELPFGWEVRLRIAVGVVKGLQYLHFTCVPQILHYN
  CRN-like gene   LKPTNVMLDAEFEPRLADYGLAKLLPNLDGGSSLYTPPECFHNCSRYTDKSDIFSFGMILGVLLT
    GKDPTDPFFGEAASGGSLGCWLRHLQQAGEAHEALDKSMLGEEGEEDEMLMAVRIAAACLSDMPA
    DRPSSDELVHMLTQLHSF
 
  Glyma08g28900   ATTATAAGAAAATAATGGTAATTTGATTTACTGAAAAAATTAGCGTCAATTCATCATTTCAATAT
  gDNA + about   TACAAAATGATACTACGATTTAGTATCTTAAGTATTGCACGCAAAAGATTATTTTCATATCATTT
  2.8 kb promoter   TTCAATTATTTAGAGAAAAAAGTGACGTTAATATCTTAGGAAAAAATTAATATCTCTGGCATTTT
  and 5′UT   AAGAAAATAAAGAGTTACTTATAAAATATGACCACGATTCATGAAATCTTATATTAAATATAGTC
  sequence (SEQ   CCGATAATTCCAATTTGTATAAACTAAAAGAATACTTATAGGAAAAAAATAGTGAGGCAAATAAA
  ID   TTAAACTTCTTTCATAAATAAAAATCAAATTATGTATTTTTACTTTTGGAAAAGTTAAATAAGAG
  NO: 47) Soybean   AATTTCTTAAAATTGATTAGATAAGTTAATTTTAACTTGTGGGAGATTTTTATTTATTTATTTTT
  CRN-like gene   CATTATACCTCTATTTTTTCTGAGTATTTTTTGAAAATTTTATCTAAATTTAAATTAAAAATTGT
    GGAGAATACTTTCAAGGAAAATGGCCTAATGGTTTAGCGTGTGTTTTAAGCATAAATTTTGGTAC
    CCATGTTTGATTAACTATTAATTAAAATTAATTTTAAAAGGCCAAGGTCATTTTCACACAATTCT
    ATTCCCTTGCACTAGACCACTTTTTAAGTATAAATTTATGACTAATGGGTCAAAGCATACAATGC
    CTTGTGTAAATAGTTGACTATCAACCAAAAATTTGACATTCAATAAGACACCACTGGTCTTTGAG
    CGACATCAATATTTTTATGAAAACGATAGTTGCACCTAATATGTGAAATTCGCAATCTGAATTAT
    TTATAAAACGTTGCATTTGCGTTCCAAATAAAAAACTCAACCCAACAAGGAAAAAAAAAACTGAA
    CTTATGTCTTGGTTTTGTTTTGTTTGGTTATTAAAAAAAGTAAATGGAACTGACTGTATCCAATT
    ATATGTCTGTTTTTAGATTTTGAAAGAAATAATTTTAAACCAATAAATATAGTTATGATATATAT
    TTAAATTAATCTCAGCTATATATTAAAATGTATATCACGGTAAAAATAATTAACTCTAGTAAATT
    ATCAAATGGATATTTGCTATAATAATTATTTGTAAATGCCTGATTTAGATTATAGTAATTTAAAA
    TCTAAGTACTTGTCATTTTTCATTTCAAAATGCCTCATGCCATAAATTAACCAAACAAACATGAA
    TTATATCCTTTGATAATTTATTCATACTATTATTGCTTGCACCTGTACATATATGTGTTGCTCAT
    TCACTCACCCCAGACTGAGTGCTAGATCCTCGGACTCGAATAAATCATTTATTATGCTTAAATAA
    TTCGATTTTATTTTTCCTACATCACAATAATCTAAAGAGTTAATCTCATACTCTCGATTCATCCT
    TAAAAATTTAATGGGTGTAATGAGAAATTAATTTTAACTACTATTATATTTTAAAAAATAAATAG
    TGAAAATAATGAGAAACTCTAATTAAAATTACTCTTTGAGTAACTAACTTGATTTTTCCTCGTAG
    AACAACCCAATCAATGTCACAACGAATTTTTCCTATTTCACGAGAGAACTTGAAAGTTAAAATTT
    GGTTAAAGTCGTTCCTAAACGTAGCAGAAGATAAGATAGAAAGATGGAAAAGTAAAAGCATGCAA
    ATATATAATTGAAATTGAAATGTAAGTAGTAGTAATTAAAAACAATTATTTGATGGAAAAAGAGA
    TAACTTTACTAATGACACACACCAAACATAAGATGTGTTCGCTAGAGTTGTTAACCACACTCACT
    CATATACAGCATATCACAAATTCCCATGCACCCTCAATTCAACGGTCCAGATGCGGTCTGATGAA
    ATCACACGGTCGATACGAACTGTACATTCTCCCTCTCTCTATCACGGATTTCGATGTTTCGCTTT
    CGGACCAAATGTGGGGCCCACATAGTACTGTGTCCTGAGTGCTGGCTACTCACAAAGGCGGGAAC
    CAGTTTTTGTCGCAGAGGTATGGCTCTTTGTTGTCATCGGATGAGAGAGAAAGAGTGTAGAGAGA
    GAAACAAAACTAAGAGACAATCACTGAATCACTCTCACTCACTCTACATGCTGTGTGCGTGACTC
    TGTCACTGTGTTTTGTGTTTAAGCACATTGCATTTTAGTTTCAGAGGAGTTTTTTTTTTTTTTTT
    TGCTGTTATTGTTATTCAAGTTTTGGTTACTACTACCGCCACATGTTCATGCCCCTTCAATTTTT
    GTTCAACTTTTTGACTTTCTGCTTGGTTTCCAAGGAAAAAGATTGCAACTTGTTTCTGGGTCTAG
    TTTGCTTTTGGTTGGGTTTGTTAGTCCCTGCTGGCACCTCGGAATAGTGGGTTTTTGTTTTTGTT
    TTTGTTTTTTTTCTTCTTTTGGAGGTTCAAATTCTTGTTCTGATTCGTGTGAAGGTGGAAAATTT
    ATGGGTGGTCACCGGAAGAGGAAAAAGATGGGATTCGTTGGAAAAAAGTAAGACTATTCGGTGAT
    AACATGTCTGCTTGCTTTTTTGGGACGGCTTTTTTGTTAAGATTTTGGGTTGAAAAGGTTGAGGA
    AGATGCTTATGCTTGCAACTTTTTTTTAAACCCATTTTAGCACCAAGTATAAAAAGTTGTTCTTG
    GTCTTGTTTCCAAGTGTTGAGGTAGGTGATAGGGGTTTTGAGCATGTGAGTGATTCATGCCTCTC
    ATTTTGGAGCTTCTGAGATTGGTTTCTGGTTGTGGCTTCGTTTGTTTGTTTGTTTGTTTGTTGTG
  CTTTCATGTTTAGGAAAAGGCACATCCTTTCTTCTCTTGCAAGGGAATTGTTGGCACTCCAGCCA
    CTTTTTCTTCTCTTCTTGTTCAGCTTGCACCACAACACTGTGCAGTGTCAAGGAAGGTTGAGTAA
    GCATGTTTCTTCAGAGCCTCCCTCACCTTCTAGGCCATCGTCAGCAGCACCATCTTCATCAGGAT
    ACAAGGATGACCCTAGGAAGATAATTTTGAGCATGGTTTTAGGAGCAGTCACTGGGCTAGTTTGT
    TCTGTTCTGTTTGCACTTGTGGTTCGTTGTGTTGTTCAGTATCTGAACCGCACACCAATCCTCAA
    GGGCCCTGTCATATTCTCCCCCAAAATTGCCTCCAAGACACTCCAATCAGCTTTGGCAAAGGAAA
    ACCACTTGCTTGGCTCGAGTCCTAATGGGAAGTACTATAAAACTATGCTTGACAATGGACTCACT
    ATTGCAGTCAAAAGGCTAACACCCTTTGAGAGCAATTCCCCGGAGGCCAAGAGGAAATCAGTGAA
    GAGGCAGATACAAACTGAGCTTGAACTTCTTGCAAGCCTTAGGAATAGGAACCTGATGAGTTTGA
    GAGCCTATGTTCGTGAGCCTGATGGATTCTCATTGGTTTATGATTATGCGTCCACTGGGAGTCTT
    GCTGATGTGTTGAATAGAGTGAGGGAGAATGAGTTGCCCTTTGGTTGGGAAGTTAGGCTCAGGAT
    TGCTGTTGGTGTGGTGAAGGGTCTTCAGTATCTTCACTTCACTTGTGTGCCTCAGATTCTGCACT
    ACAACTTGAAGCCCACTAATGTGATGTTGGATGCTGAGTTTGAACCTAGATTAGCAGATTATGGC
    TTGGCTAAACTTCTGCCTAACTTGGATAGAGGAAGTTCTCTCTACACCCCTCCTGAATGTTTCCA
    CAATTGCAGGTAAGACAAATCAATTGCTTTCAATCATACTCACTCACTAGTGTTTTGAACTTGGT
    TTGTTTCTGTTTTTTCACTTTTTACACCAAATGGGTAACTAGTTGGTTGATATTGGGCACTTGCT
    TGATTCGTTACCTTTTTAAAAGCTCCACTCCTCATTGGTTTTTTCTCCTTCTTTGGAGTACCTTA
    ATCAAAGACTCTTAGTGTGAAACGTGATTATTGTTCTGTATTGTCATGGTGTCATTTGCTATTGT
    TTAATAATTAAGACTTTGCAAAACTAATGTTTTTGTAACTACCCATTACTTGTATAGTTCACATG
    CTGCAAACTACTAAACCTAGATTGGTGATTGAGACCCCAATTAAAAATTATAATAATAATTTACT
    AAGGTTTTTCTTTTCCAATTTAACTTATTTCTAGTTTTTCATTGTTGTGTATATCTCTGGATACA
    TCAATCTTAATAGTAATAACTTAAAAATAAGTAATAACAATAAAAAGATTGCTTGATTGATGCAT
    TTCATATATGGGTATGGTATTGCCAATAAGATGTTAATTTTAACTTCATTCCATTCTTGTATGTG
    AAACTTCATGGTATTTAGATTGGATGGTGTTTTTTGCAATTTCAGCAGGTACACCGACAAAAGTG
    ATATCTTCAGTTTTGGCATCATACTAGGTGTTTTATTAACCAGTAAGGACCCTACAGATCCATTC
    TTTGGAGAAGCAGCCAGTGGGGGAAGTTTGGGATGTTGGTTGAGACACTTGCAGCAAGCCGGTGA
    GTCACGTGAAGCTCTAGATAAGAGCATGTTAGGAGAAGAAGGTGAGGAAGATGAGATGCTAATGG
    CTGTTAGGATTGCTGCTGCATGCCTTTCTGATATGCCTGCAGATAGGCCTTCTAGTGATGAGCTT
  GTTCACATGCTAACGCAACTGCACAGTTTTTGAAACAAACCTTGATTCTTCAGTTCCTAGATATT
    TTTTTCTTTCTCTTATCCCCTCTTTCTGTAATAAGATGATAGGGGAATTTGGTTAGTGCCCATGA
    TTCTGGTGTAATTGATTGTTTTGGTGTAATTGATTGTTTTGCATGATCTTGGTTTTCATGGTGTG
    GTTTCTAATATTCCATTTTCTCTTTCTCTATTCTATTTCCTTTTTCTTTTGGCTGATTTTGCAGG
    TTGTGGTGGGTTTAGGTCACACTATTATATTTTGTTTGTAAATGACTAGTCATGTTAACAAGAGT
    TTTCTTTTCTTGCT
 
  Glyma08g28900ATGTTTAGGAAAAGGCACATCCTTTCTTCTCTTGCAAGGGAATTGTTGGCACTCCAGCCACTTTT
  cDNA (SEQ ID   TCTTCTCTTCTTGTTCAGCTTGCACCACAACACTGTGCAGTGTCAAGGAAGGTTGAGTAAGCATG
  NO: 48) Soybean   TTTCTTCAGAGCCTCCCTCACCTTCTAGGCCATCGTCAGCAGCACCATCTTCATCAGGATACAAG
  CRN-like gene   GATGACCCTAGGAAGATAATTTTGAGCATGGTTTTAGGAGCAGTCACTGGGCTAGTTTGTTCTGT
    TCTGTTTGCACTTGTGGTTCGTTGTGTTGTTCAGTATCTGAACCGCACACCAATCCTCAAGGGCC
    CTGTCATATTCTCCCCCAAAATTGCCTCCAAGACACTCCAATCAGCTTTGGCAAAGGAAAACCAC
    TTGCTTGGCTCGAGTCCTAATGGGAAGTACTATAAAACTATGCTTGACAATGGACTCACTATTGC
    AGTCAAAAGGCTAACACCCTTTGAGAGCAATTCCCCGGAGGCCAAGAGGAAATCAGTGAAGAGGC
    AGATACAAACTGAGCTTGAACTTCTTGCAAGCCTTAGGAATAGGAACCTGATGAGTTTGAGAGCC
    TATGTTCGTGAGCCTGATGGATTCTCATTGGTTTATGATTATGCGTCCACTGGGAGTCTTGCTGA
    TGTGTTGAATAGAGTGAGGGAGAATGAGTTGCCCTTTGGTTGGGAAGTTAGGCTCAGGATTGCTG
    TTGGTGTGGTGAAGGGTCTTCAGTATCTTCACTTCACTTGTGTGCCTCAGATTCTGCACTACAAC
    TTGAAGCCCACTAATGTGATGTTGGATGCTGAGTTTGAACCTAGATTAGCAGATTATGGCTTGGC
    TAAACTTCTGCCTAACTTGGATAGAGGAAGTTCTCTCTACACCCCTCCTGAATGTTTCCACAATT
    GCAGCAGGTACACCGACAAAAGTGATATCTTCAGTTTTGGCATCATACTAGGTGTTTTATTAACC
    AGTAAGGACCCTACAGATCCATTCTTTGGAGAAGCAGCCAGTGGGGGAAGTTTGGGATGTTGGTT
    GAGACACTTGCAGCAAGCCGGTGAGTCACGTGAAGCTCTAGATAAGAGCATGTTAGGAGAAGAAG
    GTGAGGAAGATGAGATGCTAATGGCTGTTAGGATTGCTGCTGCATGCCTTTCTGATATGCCTGCA
  GATAGGCCTTCTAGTGATGAGCTTGTTCACATGCTAACGCAACTGCACAGTTTTTGA
 
  Glyma08g28900   MFRKRHILSSLARELLALQPLFLLFLFSLHHNTVQCQGRLSKHVSSEPPSPSRPSSAAPSSSGYK
  protein (SEQ   DDPRKIILSMVLGAVTGLVCSVLFALVVRCVVQYLNRTPILKGPVIFSPKIASKTLQSALAKENH
  ID   LLGSSPNGKYYKTMLDNGLTIAVKRLTPFESNSPEAKRKSVKRQIQTELELLASLRNRNLMSLRA
  NO: 49) Soybean   YVREPDGFSLVYDYASTGSLADVLNRVRENELPFGWEVRLRIAVGVVKGLQYLHFTCVPQILHYN
  CRN-like gene   LKPTNVMLDAEFEPRLADYGLAKLLPNLDRGSSLYTPPECFHNCSRYTDKSDIFSFGIILGVLLT
    SKDPTDPFFGEAASGGSLGCWLRHLQQAGESREALDKSMLGEEGEEDEMLMAVRIAAACLSDMPA
    DRPSSDELVHMLTQLHSF
 
  Glyma18g47610   CAAATGGGTATGCTCCCTTCAGGGGACTCCCCAATCGCCCTAATCGCAGACTCCACCGTCTCACT
  gDNA + about   CTCGTGAAACTCCGCCAGCTCCGGCTTCCCCACCGTCAGATCGCCCACCACGTGGTACACGAACA
  4 kb of   CCGACGCCATCGGAATCCAAAAGGGTATCCGGAACCACAATCAAAATCGATTTTTGTTCTGCTTT
  promoter and   TTGTATCCTTAAAAAAAAAACCGAAAACAGAAAGAAAAAAAAAAGTTTGCTTTTTTTGCTTTGTC
  5′UT sequence   GGGTGAGAGCTATAAGAGGGTATGGAGGAAGATGAGGAGAAGATCGAGGGCGGTGATGGGAGGGC
  (SEQ ID   GGTGGAGGATCACGGCAGAGAAAGAGTTAGCCATTGCCATGGAGGGAGAACGAAAAGGTTAAGGC
  NO: 50) Soybean   CCATTCAATTGAATCAGATCAGAGAGAGAGAGGGCGTAGCTTTTGGGGAAGATATGATATGTAGA
  CLV2-like   GATTTGGATAAGGTACGTCCTTTCGGGGACAGCAAGAGATGCAACGACAGAAGAAGATGGATCAG
  sequence   CGACGCTTGATGCGGTTGGGACCTGAGAATGAATGGGACACCAGACACACACTAAAAGGAGGTTC
    AATTTATCAAATAAAAAAGAGAAAGGCACAGGGGATGTGTCATGTGTCATGTGTCATGTGTCATG
    TGTATGGTGAGCTGCATCATATAGAGAATCTTTTCACCTTAATTAATTTGTTTAGTTTAATACGT
    TTTTCTTTTCTTGTCATACTCATCTTTGATTTCAATTCTATAGACCTATATATAAGTTAATTTAT
    TTAATAAGAGAGGATAAACAAAGAATGAAAATAGGTAAATGAGAAAAAAGGAGAAATAAATTAAA
    AACAATGCTTGTTTGAATTTAAAGAAACGGAAGAAAAATAAGAAAAATAGATTACTAATATAAAA
    TATCCTTTATATTACATAATTTTTTTCATATAACATAGTACATACGGACAAAACTTAGATACATT
    ATTTTGGGTGTTATTTTTTTATTAGAGTTAAAGTTTCATTTCAATGATATATATATAAGTTTTAA
    ATGTAAAACTTTATTATGCAAATTACTCAAATAAAACTCCAATTTTCATTAGAGAATAATACAAA
    CCGTGTAACGACTACAAGTTTATCTTAAATTTCCAATCTTTGAAATTATGTTATTTGTCTCCCTT
    TCTTAAAAATATAAAATTGATTTAGTGATAAAGAAAAAAGAGGAGAAGGGATAAGTTTTAAATAT
    AAATTCTTCAGGTTATAGTTCAATAGGTCACCTTTAATTAATGACGTTAATTAACAGATTAATAA
    TGACTTCAGAAGCAGTGTCTATGAAGTTTATGCGAGATCACCAATGATATATGTAGTTAATAGCA
    ACAAGTTGAGGAAAGAGGTTTGGATGAATGTGTGGCTGTTTAATGTTGGGTGGTGGTGTGGTGGC
    TATGACTACGAGGTTGGTGTTGGAAAATGTTGTCAATTCAATTGGGATTCGGTTTGCAAAGTTGT
    GATAACTTTGAGTTGAATGATGGAATATTGAAATTTTCTAGGCTTAGTTAGGAATGATTGCTACA
    TGTAACAGTGATACCACAACAACAGGGATGAGGGATTGTTGGGGTTTACTTTTAAAAAATGAATG
    AATTGAATTACAATGTAAAAGTATACATATAAAACACTATTCTTGCTTCTTAAAAAAAACGTGAG
    ACAGAGAGAAAGTGAAGATGATAAGATTATAGCGCACGCGTTGGAGCGTGCATGAGTTTACTAGG
    TCTTGTACCATGCAAAAAAATTTAGGACCCTTAGATATAACAACAAGACAAGAAGATCTTTAAGA
    GTGTAACATATGGATAACATACTGTATACCAACTTTTCTTTTTAATAGTATTTCTTCTCTCTGGT
    TATAACATCATTTTAACTAATCTATGTCTGTTAAAAAAATATTAATTTAATTAATTATATTAAAT
    ATATCAATTATTTATATTTTTTATTTTTCTATCCACTTAATTTTTTATTAATGTTTTAAAAAAAT
    AATTAAGAATAAAATAATTAATGTATTAAAAATTAAAAAAATCTTATAAATCAAGACAAATAAAT
    TTATGAAAAACATCATATAATTAGTATGGGATTATGGGATGGAGTAGTATTTAACTTGTGGCTTT
    TGAAAATTACACCATATTTTCTCTCTCTCTTGACAAAATGAATGCAACTTAAAAACGTGGGATCA
    TTCTTCCTCCTGAGTCCAGAATGTTCGACCCCATTCGTACTCTGATCTATGTGTGTTTGTGGTAT
    ATCTCCGTTGTCACTTCACCATTCTAGCTTCATCAGAGAAAGTAATATATATATTTGTAAACCAA
    TTATATATATATTTGAGAGGATTTTAATTCTTACTAAAATTGTAAACCAATTAGAAATCATTTTT
    CTGTAATTTTTGTGATCTGAAATTTTCTGTTCGGGTTGGAAATGACACAAAATCGTTGGGTCTTT
    AAATGGGTTGCAACCGGATGAGAATGACCCAACTCAAGGTAGGGGATGACCAAAGCATAGCCTTT
    TAATGGGTAATGTTAAACATGATATAAATTTATAACAAATTATTTTTATGGTGTAGTGGTTAACT
    CTTTCATTAATAATAATATAGCTGGTTGTTGGTTCCATCCCACAATAAGTCAGTTTAGCTTTTTA
    TCTTCTAAAGATTTCCTGTTTTCATTTATTTTGGTTTTTTTAAAAAATAAACAATTTCGCCTTGG
    AATCGAACTCACGATATAGTGATTAGTTATAAAAAAATAATTATAAATTATTTGGTAATTTTTTT
    CTTACATTCACTCTTGTTTTGAATACTCTTCTCTTTGTGAAGTTATGAACTTTGTTCTCTTACCA
    CAAATATGATACATCTTCTTATGTTTTTTAATTTTAGATTATATTTGATAAAACTAACCAAAAAG
    ATGAAAAATATAGTCTGTTTAAAATATTTAAGATCTAAGCTTAACTCGTTACATGTGATAGACTT
    TATTTGTAGATTATACTTGATTTATTTGAAAGTTTAGCTTAACCTATTAGTTTATTTAAAGACCT
    ATTTCATATGAAAGTTTTTATATAAGTCTATTTTTTTATATTGGACAATAAATTTATAAATCGTT
    GAGAAAATTCCATGTAAACAAACTATAATCTATAAAAAAAAAAAATTTCTTTATTCAAAGCACAA
    GATAGGTGAAAATAGATGAACTAAGTTTTATAAGTGAAATTTAACATGTCATTATGATGTAAGTT
    TATCAACTTCAAGATAACTTAGTTAAAAATATAATTTTGTAATAAGTCCTCTAATTAAAACATAA
    ATTTCGCACTCAATAATTTTTTTTTAATCGTGGATCAACACTCATAATATTTTAAAAAAGTAAAT
    AATGTATTATTTTGATACATTACAATAATTTTAATATTACAAAATATTATAATTTATATTTATTT
    AAATAGGTTGATCTATTAGGTTTAAAACACTTTTTAAATAACTTAAAACCTAATTTTTTAATCAA
    ATAGACTTTTATTAAAACTTAGATATGATTTATTTTTATTTTTTTAAAAAAAACTAACCTGACTT
    GAGTTTGATATAAATTAGGTGTCAGTTTGTTTAAATTTATTTATTAAAATAAATGTTTATTTTAA
    TAAAATAAGTAATTTTATATTTGTTTAGTATATTTGTGTAAATTCTTTTTCCTTAAAAAATATTT
    TTTTCTTTTTAAAAAAAATACTTATTTTAAAATTATTTTTTTTAAAAAGAGAAACTTGAAAAAGG
    ATAAAGTGTAATGCAGTATAGAGAGAAAGAGGAGGAAGCAAAGCAAACCAAGCACAACACAACAA
    AGCCACTTTATTTTTTTGATCTAACCTAAACCCTCTTTTTCCCCTGTTGCTCTCTCACTTTATCA
    GCGTGATACAACCAACCCAAGACCAATGTGGAAGATCTTGTTCCTCTTTCCCTTCTCTTATGTCC
  ATTTCATCATGTTTTCATTCTAATCTCCAAAATCCATGCCCACCCAGTTCCTCTTTTGCTTCAAA
    CTCCTCTCCCCCTTCCTAAAAATTGCACCTTTACTCTCATGGTGATGGGACACACCACACCCCTC
    ACACTTCTCTGTGTGATTCTTCTTTTTGCAACTCCTTCTCACTCAATTGATGTTCACCCACAAGA
    CAGAATCTCACTTTCAATGTTCAGGTCATCTCTGCCAAACCCCAACCAGAGTTTGCCCAGCTGGG
    TGGGCTCCAACTGCACTTCATGGAGTGGAATCACCTGTGACAACAGAACTGGGAGGGTGCTTTCC
    ATCAACCTAACCAGTATGAACCTTTCAGGCAAAATCCACCCCAGTTTGTGCTACCTTTCATATCT
    GAACAAGTTGGGGTTGTCCCACAACAACTTCACATCCCCTCTTCCTGAATGTTTTGGCAACTTGC
    TTAACCTAAGAGCCATTGATCTCAGCCACAACAGGCTTCATGGGGGAATACCAGACTCTTTCATG
    AGGCTTAGGCACCTCACTGAGCTTGTTTTGAGTGGGAACCCTGATTTGGGGGGTCCACTGCCTGC
    TTGGATTGGTAACTTCTCTGCAAATCTGGAAAGGTTACATCTTGGTTTCTGTTCATTCAGTGGTG
    GCATACCGGAGAGCTTGCTTTACCTGAAGTCCCTCAAGTATTTGGACCTTGAGAACAACCTCTTG
    TCTGGTAACTTGGTCAATTTTCAACAGCCTTTGGTTTTGCTCAATCTTGCTTCCAATCAGTTTGC
    TGGTACTTTGCCTTGCTTTGCAGCTTCAGTTCAGTCTCTAACTGTGTTGAATTTATCTAACAATT
    CTATTGTGGGGGGACTACCTGCTTGTATTGCTTCTTTTCAAGCTTTGACTCATTTGAACCTGTCA
    GGGAACCACTTGAAGTATAGAATATATCCTAGGCTTGTGTTCTCGGAGAAACTTCTTGTTTTGGA
    CTTGAGTAATAATGCTTTGTCTGGTCCTATTCCTTGTAAAATTGCTGAGACAACTGAGAAACTTG
    GCCTTGTTCTTCTTGACCTTTCTCACAATCAGTTCTCTGGTGAAATTCCTGTGAAAATCACTGAG
    TTGAAAAGCTTGCAGGCCTTGTTTCTCTCTCACAATCTTCTCTCTGGAGAAATTCCTGCTAGAAT
    TGGAAATTTGACTTATCTGCAGGTCATTGATCTCTCACACAACTCTTTGTCTGGAACCATTCCAT
    TCAGTATTGTTGGGTGCTTTCAGCTGTATGCTCTAATACTTACTAACAACAATCTTTCTGGTGTA
    ATTCAACCGGAGTTTGATGCGTTGGATATCTTGAGGATTCTGGATATAAGCAACAACAGGTTTTC
    CGGGGCTATCCCACTCACTCTGGCTGGATGCAAATCTCTGGAGATTGTAGATTTTAGTTCCAATG
    AGCTTTCTGGATCCTTGAATGATGCAATAACCAAATGGACAAACCTCAGGTATTTGTCTCTTGCT
    CAGAACAAGTTCAGTGGAAATCTGCCTAGTTGGTTGTTCACATTTAACGCAATAGAAATGATGGA
    TTTCTCGCATAACAAGTTTACTGGCTTCATACCTGATATTAATTTTAAGGGTAGCTTAATATTTA
    ACACCAGGAATGTCACTGTTAAAGAGCCATTGGTTGCAGCAAGAAAGGTTCAACTGAGAGTTTCG
    GCGGTTGTTTCTGATAGCAATCAGCTCAGTTTCACTTATGATCTTTCCTCAATGGTTGGAATTGA
    TCTATCCAGCAATTCGCTTCATGGGGAAATTCCAAGGGGCTTATTTGGTCTAGCTGGCCTAGAAT
    ATCTGAACTTGTCATGCAACTTTCTTTACGGACAGCTTCCGGGGTTGCAGAAAATGCATAGTTTG
    AAAGCCTTGGATTTGTCACATAATTCCTTGTCTGGACATATCCCAGGAAACATTTCTAGCCTTCA
    AGATCTGTCCATTTTGAATCTTTCCTACAACTGTTTTTCTGGATATGTTCCCCAGAAGCAAGGGT
    ATGGGAGATTTCCCGGTGCATTTGCTGGAAATCCAGATCTGTGCATGGAAACTTCCAGTGGAGTA
    TGTGATGATGGAAGGACTCAATCTGCGCAAGGAAGTTCTTTCAGTGAAGATAGGATGGATGGCCC
    AATTTCTGTGGGGATTTTCTTTATCAGTGCCTTTGTTAGTTTTGATTTTGGTGTTGTGGTTCTCT
  TCTGTTCTGCCCGGGCAAGAAATTACATTCTCCAAACAAAAGTTTGATTTGATGCTTGTGACAGT
    TACAAATCTCCTGTAAATTCCATTTTGTAATTTGGTACCTGTGTTCTCAGTTTCAAGTAAAACAT
    ACACTTATGTGACTAGGAATACTATCCGGCCATCAACTTCACAAGTGTTTTCTTGTGATTCCTGA
    TCAAGTGTCTCAGATTTACAGGATCAAAATGCCATGACATGAGTAACACAAGGTTTAAAGAACAC
    TCAACACTGGCTTTATCTATCTGAGTGAAGACTAGCCTGGCATCATTCAGCCAAGAAAAGAATGG
    ATGATTATGATGAAAATTTGATCCGAGTAAAGACGAGTCCCTCATCATTCTGATGGTTGTTCTCT
    TTTGCTGGAATTTGGTTGCATCAAGTTTAGAATGCATCATCACATGTATTATTCATAATCAGTGG
    TGGGCGAAGGGTCAGTAGGGAACATGTCTGATATCTGGTCTAGTTATGGTGAAATTTTGATCTTG
    GGCATCAAATTGCAGATTTGCAAGCATGTTTACGTGAAGAGAACTTGTATAATTCTTGATTAACC
    TAGTTCTTTCTTGAGGTGGGGAACCAAGTTTTCCCTGTAAGTGGGGAGTAGGTTCTCATAAGTCT
    AAGATTTGTATTTGTATTACTATCTTCACACCTTCATCATAGTGCTGTGATTTTAAATGATATTC
    TCACGAAACCTTTTCATTGACAACAGAAAAGAGGTTAATTGA
 
  Glyma18g47610ATGCCCACCCAGTTCCTCTTTTGCTTCAAACTCCTCTCCCCCTTCCTAAAAATTGCACCTTTACT
  cDNA (SEQ ID   CTCATGGTCATCTCTGCCAAACCCCAACCAGAGTTTGCCCAGCTGGGTGGGCTCCAACTGCACTT
  NO: 51) Soybean   CATGGAGTGGAATCACCTGTGACAACAGAACTGGGAGGGTGCTTTCCATCAACCTAACCAGTATG
  CLV2-like   AACCTTTCAGGCAAAATCCACCCCAGTTTGTGCTACCTTTCATATCTGAACAAGTTGGGGTTGTC
  sequence   CCACAACAACTTCACATCCCCTCTTCCTGAATGTTTTGGCAACTTGCTTAACCTAAGAGCCATTG
    ATCTCAGCCACAACAGGCTTCATGGGGGAATACCAGACTCTTTCATGAGGCTTAGGCACCTCACT
    GAGCTTGTTTTGAGTGGGAACCCTGATTTGGGGGGTCCACTGCCTGCTTGGATTGGTAACTTCTC
    TGCAAATCTGGAAAGGTTACATCTTGGTTTCTGTTCATTCAGTGGTGGCATACCGGAGAGCTTGC
    TTTACCTGAAGTCCCTCAAGTATTTGGACCTTGAGAACAACCTCTTGTCTGGTAACTTGGTCAAT
    TTTCAACAGCCTTTGGTTTTGCTCAATCTTGCTTCCAATCAGTTTGCTGGTACTTTGCCTTGCTT
    TGCAGCTTCAGTTCAGTCTCTAACTGTGTTGAATTTATCTAACAATTCTATTGTGGGGGGACTAC
    CTGCTTGTATTGCTTCTTTTCAAGCTTTGACTCATTTGAACCTGTCAGGGAACCACTTGAAGTAT
    AGAATATATCCTAGGCTTGTGTTCTCGGAGAAACTTCTTGTTTTGGACTTGAGTAATAATGCTTT
    GTCTGGTCCTATTCCTTGTAAAATTGCTGAGACAACTGAGAAACTTGGCCTTGTTCTTCTTGACC
    TTTCTCACAATCAGTTCTCTGGTGAAATTCCTGTGAAAATCACTGAGTTGAAAAGCTTGCAGGCC
    TTGTTTCTCTCTCACAATCTTCTCTCTGGAGAAATTCCTGCTAGAATTGGAAATTTGACTTATCT
    GCAGGTCATTGATCTCTCACACAACTCTTTGTCTGGAACCATTCCATTCAGTATTGTTGGGTGCT
    TTCAGCTGTATGCTCTAATACTTACTAACAACAATCTTTCTGGTGTAATTCAACCGGAGTTTGAT
    GCGTTGGATATCTTGAGGATTCTGGATATAAGCAACAACAGGTTTTCCGGGGCTATCCCACTCAC
    TCTGGCTGGATGCAAATCTCTGGAGATTGTAGATTTTAGTTCCAATGAGCTTTCTGGATCCTTGA
    ATGATGCAATAACCAAATGGACAAACCTCAGGTATTTGTCTCTTGCTCAGAACAAGTTCAGTGGA
    AATCTGCCTAGTTGGTTGTTCACATTTAACGCAATAGAAATGATGGATTTCTCGCATAACAAGTT
    TACTGGCTTCATACCTGATATTAATTTTAAGGGTAGCTTAATATTTAACACCAGGAATGTCACTG
    TTAAAGAGCCATTGGTTGCAGCAAGAAAGGTTCAACTGAGAGTTTCGGCGGTTGTTTCTGATAGC
    AATCAGCTCAGTTTCACTTATGATCTTTCCTCAATGGTTGGAATTGATCTATCCAGCAATTCGCT
    TCATGGGGAAATTCCAAGGGGCTTATTTGGTCTAGCTGGCCTAGAATATCTGAACTTGTCATGCA
    ACTTTCTTTACGGACAGCTTCCGGGGTTGCAGAAAATGCATAGTTTGAAAGCCTTGGATTTGTCA
    CATAATTCCTTGTCTGGACATATCCCAGGAAACATTTCTAGCCTTCAAGATCTGTCCATTTTGAA
    TCTTTCCTACAACTGTTTTTCTGGATATGTTCCCCAGAAGCAAGGGTATGGGAGATTTCCCGGTG
    CATTTGCTGGAAATCCAGATCTGTGCATGGAAACTTCCAGTGGAGTATGTGATGATGGAAGGACT
    CAATCTGCGCAAGGAAGTTCTTTCAGTGAAGATAGGATGGATGGCCCAATTTCTGTGGGGATTTT
    CTTTATCAGTGCCTTTGTTAGTTTTGATTTTGGTGTTGTGGTTCTCTTCTGTTCTGCCCGGGCAA
  GAAATTACATTCTCCAAACAAAAGTTTGA
 
  Glyma18g47610   MPTQFLFCFKLLSPFLKIAPLLSWSSLPNPNQSLPSWVGSNCTSWSGITCDNRTGRVLSINLTSM
  protein (SEQ   NLSGKIHPSLCYLSYLNKLGLSHNNFTSPLPECFGNLLNLRAIDLSHNRLHGGIPDSFMRLRHLT
  ID   ELVLSGNPDLGGPLPAWIGNFSANLERLHLGFCSFSGGIPESLLYLKSLKYLDLENNLLSGNLVN
  NO: 52) Soybean   FQQPLVLLNLASNQFAGTLPCFAASVQSLTVLNLSNNSIVGGLPACIASFQALTHLNLSGNHLKY
  CLV2-like   RIYPRLVFSEKLLVLDLSNNALSGPIPCKIAETTEKLGLVLLDLSHNQFSGEIPVKITELKSLQA
  sequence   LFLSHNLLSGEIPARIGNLTYLQVIDLSHNSLSGTIPFSIVGCFQLYALILTNNNLSGVIQPEFD
    ALDILRILDISNNRFSGAIPLTLAGCKSLEIVDFSSNELSGSLNDAITKWTNLRYLSLAQNKFSG
    NLPSWLFTFNAIEMMDFSHNKFTGFIPDINFKGSLIFNTRNVTVKEPLVAARKVQLRVSAVVSDS
    NQLSFTYDLSSMVGIDLSSNSLHGEIPRGLFGLAGLEYLNLSCNFLYGQLPGLQKMHSLKALDLS
    HNSLSGHIPGNISSLQDLSILNLSYNCFSGYVPQKQGYGRFPGAFAGNPDLCMETSSGVCDDGRT
    QSAQGSSFSEDRMDGPISVGIFFISAFVSFDFGVVVLFCSARARNYILQTKV
 
[0160] Having illustrated and described the principles of the present invention, it should be apparent to persons skilled in the art that the invention can be modified in arrangement and detail without departing from such principles. Although the materials and methods of this invention have been described in terms of various embodiments and illustrative examples, it will be apparent to those of skill in the art that variations can be applied to the materials and methods described herein without departing from the concept, spirit and scope of the invention. All such similar substitutes and modifications apparent to those skilled in the art are deemed to be within the spirit, scope and concept of the invention as defined by the appended claims.
(57)

Claims

1. A method for inhibiting plant parasitic nematode damage to a potato plant, said method comprising:
(1) reducing the expression of an endogenous CLAVATA2-like (CLV2-like) gene by introducing into said potato plant one or more non-natural mutations in said CLV2-like gene, or by expressing in said potato plant RNAi to silence said CLV2-like gene, wherein said CLV2-like gene is set forth in SEQ ID NO: 11, and
(2) growing said potato plant comprising said one or more non-natural mutations or expressing said RNAi, thereby inhibiting plant parasitic nematode damage to said potato plant.
2. The method of claim 1, further comprising the step of harvesting a product of said potato plant.
3. The method of claim 2, wherein said product is a leaf, stem, flower, seed, root, or tuber.
4. The method of claim 2, wherein the yield and/or quality of said product is increased relative to a control plant not comprising said one or more non-natural mutations or expressing said RNAi.
5. The method of claim 1, wherein said plant parasitic nematode is a cyst nematode.
6. The method of claim 5, wherein said cyst nematode is a Globodera spp.
7. The method of claim 1, wherein said plant parasitic nematode is G.rostochiensis or G.pallida.
8. A genetically modified nematode resistant potato plant produced by the method of claim 1, wherein said potato plant comprises said one or more non-natural mutations or expresses said RNAi.
9. A method for inhibiting plant parasitic nematode damage to a soybean plant, said method comprising:
(1) reducing the expression of an endogenous CLAVATA2-like (CLV2-like) gene by introducing into said soybean plant one or more non-natural mutations in said CLV2-like gene, or by expressing in said soybean plant RNAi to silence said CLV2-like gene, wherein said CLV2-like gene is set forth in SEQ ID NO: 35 or SEQ ID NO: 50; and
(2) growing said soybean plant comprising said one or more non-natural mutations or expressing said RNAi, thereby inhibiting plant parasitic nematode damage to said soybean plant.
10. The method of claim 9, further comprising the step of harvesting a product of said soybean plant.
11. The method of claim 10, wherein said product is a leaf, stem, flower, seed, root, or tuber.
12. The method of claim 10, wherein the yield and/or quality of said product is increased relative to a control plant not comprising said one or more non-natural mutations or expressing said RNAi.
13. The method of claim 9, wherein said plant parasitic nematode is a cyst nematode.
14. The method of claim 13, wherein said cyst nematode is a Heterodera spp.
15. The method of claim 9, wherein said plant parasitic nematode is H.glycines.
16. A genetically modified nematode resistant soybean plant produced by the method of claim 9, wherein said soybean plant comprises said one or more non-natural mutations or expresses said RNAi.
*****

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